Wan-Li Chen

National Cheng Kung University Hospital

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Siqian Zhang

National University of Defense Technology

Wei Wu

Air Force Medical University

Chung‐Liang Ho

National Cheng Kung University Hospital

Nan‐Haw Chow

National Cheng Kung University

Xinchi Zhang

Dalian University of Technology

Cun Zhang

Air Force Medical University

Yilin Chen

Jiangnan University

Yinggang Wang

China University of Mining and Technology

Wu‐Chou Su

Dana-Farber Cancer Institute

Haibin Xu

Juntendo University

Cooperative Institutions

National Cheng Kung University Hospital

National Cheng Kung University

Air Force Medical University

Fudan University

Chinese Academy of Sciences

China Medical University

South China Agricultural University

China Medical University

China Medical University Hospital

Center for Excellence in Molecular Cell Science

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Identification and characterization of two linear epitope motifs in hepatitis E virus ORF2 protein

PLoS ONE (2017)

Heng Wang Weidong Zhang Honglang Gu Wan-Li Chen Meng Zeng

Hepatitis E virus (HEV) is responsible for hepatitis E, which represents a global public health problem. HEV genotypes 3 and 4 are reported to be zoonotic, and animals are monitored for HEV infection in the interests of public hygiene and food safety. The development of novel diagnostic methods and vaccines for HEV in humans is thus important topics of research. Opening reading frame (ORF) 2 of HEV includes both linear and conformational epitopes and is regarded as the primary candidate for vaccines and diagnostic tests. We investigated the precise location of the HEV epitopes in the ORF2 protein. We prepared four monoclonal antibodies (mAbs) against genotype 4 ORF2 protein and identified two linear epitopes, G438IVIPHD444 and Y457DNQH461, corresponding to two of these mAbs using phage display biopanning technology. Both these epitopes were speculated to be universal to genotypes 1, 2, 3, 4, and avian HEVs. We also used two 12-mer fragments of ORF2 protein including these two epitopes to develop a peptide-based enzyme-linked immunosorbent assay (ELISA) to detect HEV in serum. This assay demonstrated good specificity but low sensitivity compared with the commercial method, indicating that these two epitopes could serve as potential candidate targets for diagnosis. Overall, these results further our understanding of the epitope distribution of HEV ORF2, and provide important information for the development of peptide-based immunodiagnostic tests to detect HEV in serum.

Hepatitis E Virus

Biopanning

Hepatitis E

Linear epitope

Epitope mapping

10.1371/journal.pone.0184947

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Citations (16)

4,4-Dichlorotricyclo[5.4.0.0^3,5]undeca-7,9,11-triene

Acta Crystallographica Section E Structure Reports Online (2008)

Wan-Li Chen Guosheng Chen Yinghong Zhu Weimin Mo

The title compound, C11H10Cl2, is a useful intermediate for the synthesis of 1H-cyclopropa[b]naphthalene. Strain in the molecule is evidenced by the fact that the cyclohexane ring is essentially planar and nearly coplanar with the benzene ring [dihedral angle 1.87 (18)°], and the cyclopropyl ring is almost perpendicular to the cyclohexane ring [dihedral angle 70.99 (12)°]. The molecules are loosely connected into one-dimensional chains by intermolecular Cl⋯Cl interactions with a distance of 3.571 (1) Å. The centroid-to-centroid distance between stacked benzene rings is ca 5.89 Å, indicating that no π–π stacking exists in the crystal structure.

Dihedral angle

10.1107/s160053680801831x

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Neuroprotective effect of dimethyl fumarate on cognitive impairment induced by ischemic stroke

Annals of Translational Medicine (2020)

Xiaowen Hou Haibin Xu Wan-Li Chen Nannan Zhang Zi‐Ai Zhao

Background: Oxidative damage may contribute to post-stroke cognitive impairment (PSCI), but the underlying mechanisms are not fully elucidated. Dimethyl fumarate (DMF) has been used as an antioxidant in multiple sclerosis and psoriasis patients. We hypothesized that redox state was associated with PSCI, and DMF might exert neuroprotective effect against PSCI via anti-oxidative actions. Methods: To confirm this hypothesis, we first conducted a clinical study (NCT03519828) that enrolled patients diagnosed with acute ischemic stroke within 48 hours. Data were analyzed based on demographic characteristics, disease history, clinical data and redox state. Logistic regression was used to identify the factors associated with PSCI. Next, a middle cerebral artery occlusion (MCAO) rat model was used to explore the antioxidant capacity and neuroprotective effect of DMF. Furthermore, behavioural experiments, histology and immunostaining, and transmission electron microscopy were also performed. Results: Higher baseline NIHSS score, lower GSH/GSSG and T-AOC levels were found in the PSCI patients. Better performance in Morris water maze and shuttle box testing, more regular arranged neurons and Nissl bodies, less TUNEL-positive cells and autophagosomes, lower expression of 4-HNE, and higher expression of GCLM and NQO1 were found in the (DMF + MCAO) rats compared with the MCAO rats. Conclusions: These findings suggest that DMF may alleviate PSCI via neuroprotective actions, providing a new therapeutic strategy for PSCI.

Dimethyl fumarate

Stroke

10.21037/atm.2020.02.10

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Citations (22)

Et3N-promoted sequential reactions for the synthesis of 6H-benzo[c]chromenes

ARKIVOC (2011)

Wan-Li Chen Jiao Li Yinghong Zhu Wei Hu Weimin Mo

An interesting Et 3 N-promoted sequential reaction consisting of propargyl-allenyl isomerizations, intramolecular [4+2] cycloaddition and aromatization has been developed, providing a facile method for synthesis of 6H-benzo[c]chromenes under mild conditions in moderate yields.

10.3998/ark.5550190.0013.602

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Citations (2)

AgOTf-catalyzed cyclization of enynals or enynones with amines: an efficient synthesis of 1,2,4-trisubstituted pyrroles and 2,3,5-trisubstituted furans

ARKIVOC (2011)

Wan-Li Chen Jiao Li Yinghong Zhu Lu-Ting Ye Wei Hu

AgOTf-catalyzed amino-cyclization of 2-(1-alkynyl)-2-alken-1-als or 2-(1-alkynyl)-2-alken-1ones is presented, providing an efficient method for synthesis of 1,2,4-trisubstituted pyrroles or 2,3,5-trisubstituted furans with an arylamino-group function respectively under mild conditions in moderate yields.

10.3998/ark.5550190.0012.928

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Citations (23)

Recombinant DTβ4-inspired porous 3D vascular graft enhanced antithrombogenicity and recruited circulating CD93 ⁺ /CD34 ⁺ cells for endothelialization

Science Advances (2022)

Weiwei Xiao Wan-Li Chen Yinggang Wang Cun Zhang Xinchi Zhang

Matching material degradation with host remodeling, including endothelialization and muscular remodeling, is important to vascular regeneration. We fabricated 3D PGS-PCL vascular grafts, which presented tunable polymer components, porosity, mechanical strength, and degrading rate. Furthermore, highly porous structures enabled 3D patterning of conjugated heparin-binding peptide, dimeric thymosin β4 (DTβ4), which played key roles in antiplatelets, fibrinogenesis inhibition, and recruiting circulating progenitor cells, thereafter contributed to high patency rate, and unprecedentedly acquired carotid arterial regeneration in rabbit model. Through single-cell RNA sequencing analysis and cell tracing studies, a subset of endothelial progenitor cells, myeloid-derived CD93 + /CD34 + cells, was identified as the main contributor to final endothelium regeneration. To conclude, DTβ4-inspired porous 3DVGs present adjustable physical properties, superior anticoagulating, and re-endothelializing potentials, which leads to the regeneration of small-caliber artery, thus offering a promising tool for vessel replacement in clinical applications.

10.1126/sciadv.abn1958

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Citations (15)

The rate of early neurological deterioration occurring after thrombolytic therapy: A meta‐analysis

Brain and Behavior (2019)

Xiaowen Hou Wan-Li Chen Haibin Xu Zhigang Zhu Yuanyuan Xu

Abstract Objectives The rate of early neurological deterioration (END) occurring after thrombolytic therapy is controversial. To explore a more precise estimation of the rate, a meta‐analysis was conducted in the present study. Methods The relevant studies were identified by searching PubMed, EMBASE, and Cochrane Collaboration Database up to June 2018. The definition of END was prespecified according to the most commonly used definition: ≥4‐point increase in National Institutes of Health Stroke Scale between admission and 24 hr. The meta‐analysis was performed by using the STATA 12. Results Eleven studies with a total of 3,539 subjects, including 373 patients with END and 3,166 patients without END, were collected. The pooled analysis showed that the rate of END occurring after thrombolytic therapy was about 11.0% (95% CI: 7.8%–14.3%). Subgroup analysis by continent showed that the rate of END occurring after thrombolytic therapy of patients in Asia (15.9%, 95% CI: 7.4%–24.5%) was higher than in Europe (7.6%, 95% CI: 4.9%–10.3%) and in North America (11.8%, 95% CI: 8.5%–15.0%). Subgroup analysis by onset to treatment time (OTT) displayed that the rate of END occurring after thrombolytic therapy was 5.4% (95% CI: 1.2%–9.5%), 15.6% (95% CI: 9.6%–21.5%), and 18.5% (95% CI: 11.2%–25.8%) for the patients whose OTT ≤120.0 min, from 120.1 to 179.9 min, from 180.0 to 270.0 min, respectively. Conclusion The rate of END occurring after thrombolytic therapy is about 11.0%. This finding may provide a scientific reference for researchers to evaluate the efficacy and safety of thrombolytic therapy.

Subgroup analysis

Stroke

10.1002/brb3.1210

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Citations (13)

Epidemiology of pandemic influenza A/H1N1 virus during 2009–2010 in Taiwan

Virus Research (2013)

Yu‐Ching Lan Mei-Chi Su Chao‐Hsien Chen Su-Hua Huang Wan-Li Chen

Outbreak of swine-origin influenza A/H1N1 virus (pdmH1N1) occurred in 2009. Taiwanese authorities implemented nationwide vaccinations with pdmH1N1-specific inactivated vaccine as of November 2009. This study evaluates prevalence, HA phylogenetic relationship, and transmission dynamic of influenza A and B viruses in Taiwan in 2009-2010. Respiratory tract specimens were analyzed for influenza A and B viruses. The pdmH1N1 peaked in November 2009, was predominant from August 2009 to January 2010, then sharply dropped in February 2010. Significant prevalence peaks of influenza B in April-June of 2010 and H3N2 virus in July and August were observed. Highest percentage of pdmH1N1- and H3N2-positive cases appeared among 11-15-year-olds; influenza B-positive cases were dominant among those 6-10 years old. Maximum likelihood phylogenetic trees showed 11 unique clusters of pdmH1N1, seasonal H3N2 influenza A and B viruses, as well as transmission clusters and mixed infections of influenza strains in Taiwan. The 2009 pdmH1N1 virus was predominant in Taiwan from August 2009 to January 2010; seasonal H3N2 influenza A and B viruses exhibited small prevalence peaks after nationwide vaccinations. Phylogenetic evidence indicated transmission clusters and multiple independent clades of co-circulating influenza A and B strains in Taiwan.

Pandemic

H1n1 pandemic

Pandemic influenza

10.1016/j.virusres.2013.07.007

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Citations (16)

Fast degrading elastomer stented fascia remodels into tough and vascularized construct for tracheal regeneration

Materials Science and Engineering C (2019)

Wei Wu Sansan Jia Wan-Li Chen Xuzheng Liu Siqian Zhang

Electrospinning

10.1016/j.msec.2019.02.108

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Citations (16)

The impact of social capital on physical activity and nutrition in China: the mediating effect of health literacy

BMC Public Health (2019)

Wan-Li Chen Chenggang Zhang Zi-Yi Cui Jingya Wang Jie Zhao

Physical activity and good nutrition are important behavioral factors in promoting health and preventing disease. It is important to understand the factors affecting physical activity and nutrition. The purpose of this study was to explore whether social capital has an effect on physical activity and nutrition, and whether health literacy plays a mediating role between social capital and physical activity as well as nutrition.This cross-sectional study was performed in a certain district of Shanghai in March and April 2017. Data was collected using a self-reported questionnaire, which included questions on sociodemographic characteristics, social capital, health literacy and health-promoting lifestyle profile-II. Health-promoting lifestyle profile-II measures the behaviours or habits of physical activity and healthy nutrition. An explore factor analysis of the principal components with varimax rotation was carried out on the social capital scale. Descriptive statistics was used to summarize the sociodemographic of participants. Mediation analysis was performed using the bootstrapping tests to examine whether health literacy mediate the relationship between social capital and physical activity as well as nutrition.The explore factor analysis results showed that social capital has five dimensions, namely social participation, social support, social network, control over life and feelings about the community. There is a positive correlation between social capital, health literacy, physical activity and nutrition. The correlation coefficient varied from 0.135 to 0.594. Mediation analysis demonstrated health literacy played a partial mediating effect between social capital and physical activity as well as nutrition. In the relationship between physical activity and social capital, the indirect effect of health literacy accounted for 8.20 to 12.65% of the total effect. In the relationship between nutrition and social capital, the mediation effect of health literacy accounted for 4.93 to 12.71% of the total effect.Social capital can promote physical activity and nutrition by disseminating health information. Enhancing the social capital of residents will help increase physical activity and develop healthy eating habits. Attention should also be paid to the improvement of residents' health literacy.

Health Literacy

Social Engagement

10.1186/s12889-019-8037-x

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Citations (50)