Jaime Eduardo Muñoz Flórez

Universidad Nacional de Colombia

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Luz A Álvarez

Corporación Universitaria del Huila

Andrés Mauricio Posso Terranova

Universidad Nacional de Colombia

Herney Darío Vásquez Amariles

Universidad Nacional de Colombia

Franco Alirio Vallejo Cabrera

Universidad Nacional de Colombia

Diana López

Harvard University

Andrés Posso

Universidad Nacional de Colombia

Martha Liliana Bonilla Betancourt

Universidad Nacional de Colombia

Darwin Hernández-Herrera

Universidad Nacional de Colombia

Juan Diego Duque-Zapata

Universidad Nacional de Colombia

Ana Cruz Morillo Coronado

Pedagogical and Technological University of Colombia

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Universidad Nacional de Colombia

106

International Center for Tropical Agriculture

Colombian Corporation for Agricultural Research - AGROSAVIA

Universidad del Valle

Pontificia Universidad Javeriana

Centro de Investigación de la Caña de Azúcar de Colombia

University of Nariño

Universitat Politècnica de València

University of Córdoba

International Center for Tropical Agriculture

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Failure mechanisms of curved trapezoidal steel sheeting

Miguel Casafont Ribera Miquel Ferrer Frederic Marimon Pedro Casariego Vales Jaime Eduardo Muñoz Flórez

Cold-formed curved steel sheets were first used to protect some parts of buildings from external agents (i.e. corner roofs, parking columns). Since a few decades ego, steel sheeting manufacturers have produced a new application: cold-formed self carrying arches (Fig. 1). Generally speaking, nowadays two main types of cold-formed curved profiles can be found in the market: (i) The first type consists of ribbed sheets bent to an arch (Fig. 2). The span of these arches commonly ranges from four to eight meters. The present investigation is focused on arches of this type bent by pressforming. (ii) The second type is obtained by folding a flat steel sheet to form a large rib (usually a large omega) and, afterwards, press-forming it into a curved shape. The spans of the arches produced this way range from 20 to 30 m.

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CARACTERIZACIÓN MOLEC u LAR DEL BANCO DE GERMOPLASMA DE G u AYABA Psidium spp. DEL CENTRO DE INVESTIGACIÓN CORPOICA-PALMIRA

Antoni Rueda Juan Diego Palacio Jaime Eduardo Muñoz Flórez R Said Saavedra Enrique Díaz Bravo

RESuMEN A pesar de ser la guayaba Psidium spp . en el pais una fruta promisoria en el mercado nacional y con potencial internacional, su cultivo posee una serie de limitantes, ya que la produccion se hace de manera artesanal y son necesarias variedades seleccionadas segun criterios de mercado y con caracteris-ticas agronomicas deseables. Existe una gran oferta de germoplasma de guayaba que es necesario explorar para ofrecer alternativas a los productores. Como aporte a esta explora-cion, se desarrollo este estudio, encaminado a caracterizar molecularmente con la tecnica de RAPD (Random Amplified Polymorphic DNA ) 27 accesiones del banco de germoplasma de guayaba del Centro de Investigacion (CI) de Corpoica en Palmira. Se evaluaron seis marcadores que generaron un total de 43 loci polimorficos. El analisis de similaridad y de correspondencia multiple permitio separar las accesiones, por origen geografico, detec-tando un material replicado, un gran grupo (20 accesiones) que reune las accesiones recolectadas en el continente americano, asi como la separacion de las accesiones de guayaba colectadas en Africa y Suriname, la guayaba coronilla, la peruana y manzana formando un grupo individual cada una. Este estudio permite apreciar el relativo alto grado de diversidad genetica al interior del banco de germoplasma de guayaba de Corpoica en Palmira.

Germ plasm

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Caracterización palinológica y viabilidad polínica de Physalis peruviana L. y Physalis philadelphica Lam. Physalis peruviana L. and Physalis philadelphica Lam. palynological characterization and pollen viability

Agronomía Colombiana (2005)

Túlio César Lagos Creuci María Caetano Franco Alirio Vallejo Cabrera Jaime Eduardo Muñoz Flórez Hernando Criollo

Physalis peruviana L. and P. philadelphica Lam. represent two Solanaceae species which are economically important for Colombia and Mexico, respectively. Little is currently known regarding P. peruviana and P. philadelphica palynology and pollen viability. This work was thus aimed at characteris- ing these two species' pollen grains and determining pollen viability in contrasting cold and warm climate conditions. Acetolysis (light microscopy) and scanning electron microscopy were employed for pollen char- acterisation. The following traits were determined: polar axis, equatorial diameter, aperture number, position and character (NPC), apocolpus/apoporus side, pollen morphology, axis and equatorial contour, polar area and aperture and pollen types. This led to describing and characterising the pollen grains of the genotypes being studied. Flower fixation during anthesis, followed by acetocarmin staining, was used for establishing pollen viability; observations were made by light microscope. The two species displayed a medium-sized pollen grain zono-tricolporate in P. peruviana and a small-sized one in P. philadelphica. Mar- ginal environments reduced pollen viability but did not affect fertility.

Physalis

Anthesis

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Morphological characterization of wild accessions of guava.

Liliana Jiménez Lozano Magda I. Pinzón Jaime Eduardo Muñoz Flórez

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Detección de una mutación puntual en el gen receptor Ryanodina (Ryr 1) en cerdos criollos colombianos

DOAJ (DOAJ: Directory of Open Access Journals) (2008)

Darwin Hernández-Herrera Andrés Mauricio Posso Terranova Jaime Eduardo Muñoz Flórez

El sindrome de estres porcino (PSS) es una enfermedad hereditaria monogenica recesiva relacionada con el gen receptor ryanodina (Ryr1). Utilizando PCR-SSCP y PCR-RFLP se tipificaron geneticamente 14 individuos de cerdos comerciales con el rasgo sindactilia (Casco de Mula-CM), 21 San Pedrenos -SP y 100 Zungos- ZN. Las razas CM y SP tuvieron las mismas frecuencias alelicas (F(H) = 0.79 y F(h) = 0.21), mientras que en los cerdos ZN no se encontro el alelo recesivo (h). La heterocigosidad (He) fue de 0.28% para los cerdos CM y 0.23% para los SP. La He para la muestra poblacional fue de 0.066.

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Caracterización morfológica en árboles nativos de guayaba en el Valle del Cauca

Acta Agronómica (2005)

O Hilsy L Sanabria Mario A. García Hermosilla Díaz Jaime Eduardo Muñoz Flórez

Se caracterizaron 53 accesiones de Psidium guajava, colectadas en 9 transectos del Valle del Cauca. En los descriptores cualitativos las accesiones se integraron en tres grupos que se diferenciaron por la forma del fruto. El 75% de los descriptores cuantitativos mostraron CV mayor al 24%. El 72.41% de la variacion total de estos descriptores fue explicado en tres CP, que permitieron discriminar para variables de rendimiento del fruto, estructura del arbol y calidad del fruto. Abstract Morphological characteristics of guava in Cauca Valley, Colombia. 53 accesions were collected of Psidium guajava, in 9 transects of the Cauca Valley, these themselves were characterizates. In the descriptors qualitative the accesions were integrated in three groups that were differentiated for the form of the fruit. The 75% of the descriptors quantitative they showed greater VC to 24%. The 72.41% of the total variation of these descriptors, was explained in three PC, that permitted to discriminate for variables of performance of the fruit, structure of the tree and quality of the fruit. Keywords: Psidium guajava, accesions, descriptors quantitative, descriptors qualitative. Principal Component Analysis - PCA

Psidium

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Genetic diversity and geographical genetic diversity in Colombian accessions of Lippia alba (Mill.) N.E. Brown

Revista de Ciencias Agrícolas (2019)

José Omar Cardona Montoya Jaime Eduardo Muñoz Flórez

Bushy matgrass (Lippia alba, Verbenaceae) is a promising plant genetic resource, by their active compounds. The present document studied the molecular genetic diversity and spatial genetic structure of two contrasting populations of L. alba in Colombia. Eight RAM, evaluated total DNA of 59 accessions of non-cultivated plants collected in two Colombia regions, Chicamocha and Sumapaz. The expected average heterozygosity (or average heterozygosity genetic diversity of Nei) for the sample had low value (0.0≤He=0.2467≤0.5). The values of molecular diversity (MD) indicated values in the range of 0.1219 to 0.3425 for seven RAM. The frequency of variants is based on an effective number of alleles [Ae] and expected heterozygosity [He], genetic diversity by locus (hj=1-p2-q2) had maximum values (near 0.5) in the primers ACA, AG, CGA, and CEC. RAM suitably analyzed Lippia alba as an endemic genetic resource. A DNA bank composed of 59 Colombian accessions from Lippia alba was set up. The analysis of the spatial global structure shows that the subpopulation Sumapaz is structured, whilst the subpopulation Chicamocha, is in the structuring process. The results suggest in all cases the need for implementing: a) exchange of gene-seed, (b) gene banks with maximum genetic variability and c) induce genetic diversity.

10.22267/rcia.193602.117

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Expresión transitoria del gen GUS en caña de azúcar usando Agrobacterium tumefaciens Transient gene expression in sugarcane using Agrobacterium tumefaciens

DOAJ (DOAJ: Directory of Open Access Journals) (2008)

Martha Liliana Bonilla Betancourt Jaime Eduardo Muñoz Flórez Fernando ángel Sánchez

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Molecular and morphological characterization of Musa spp. (Zingiberales : Musaceae) cultivars.

Boletín Científico Centro de Museos Museo de Historia Natural (2020)

María Angélica Buitrago Bitar Ayda Lilia Enríquez Valencia Jorge Mario Londoño Jaime Eduardo Muñoz Flórez Bernardo Villegas Estrada

Objectives: The overall goal was to analyze genetic diversity in cultivars of Musa acuminata (Colla) and M. balbisiana (Colla), commonly grown in farms from Caldas department. Scope: Characterization of the genetic variability, at the molecular and morphological level of cultivars of M. acuminata and M. balbisiana, found in farms from Caldas farmers using morphological descriptors and fluorescent microsatellites. Methodology: Phenotyping evaluations comprised 57 morphological characters following the descriptors proposed by IPGRI for the Musa genus, and for genotyping evaluations, nine fluorescent microsatellites (Simple Sequence Repeats-SSR) were used to allow the precise identification of alleles. Additionally, cluster analyses were carried out independently for both morphological and genotypic characterizations under Principal Component Analysis (PCA) and Bootstrapping methods respectively. Main results: Positive and negative highly significant correlations were found for the morphological descriptors, where traits such as presence/ absence of male bud was the rule, as well as the diameter and perimeter of this trait, plus the diameter and perimeter of the peduncle, number of fruits, pseudostem height and fruit length contributed considerably to the variability among the cultivars allowing the discrimination of three main groups in the cluster analyzes. From the molecular perspective a total of 72 polymorphic alleles were obtained, with an average genetic diversity of 0,79, polymorphic information content (PIC) of 0,77 and heterozygosity of 0,48, showed a moderate degree of genetic differentiation (FST = 0,061) among Musa cultivars, generating three main sub-clusters based on their genetic dissimilarity. Conclusions: The identification of certain morphological traits showed to be suitable for the discrimination of Musa cultivars evaluated here. On the other hand, molecular characterization allowed to establish the genetic relationships among groups, also fluorescent SSR were highly informative and accurate, in such a way that can be considered suitable for characterizations in Musa varieties.

Musaceae

Musa acuminata

10.17151/bccm.2020.24.1.2

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Lower coronary disease in hispanic countries due to admixture of genes-in Puerto Rico 3 protective genes

Atherosclerosis (2018)

Pablo I. Altieri H.L. Banchs Jaime Eduardo Muñoz Flórez

10.1016/j.atherosclerosis.2018.06.722

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