Marta Svetlikova

University of Tennessee at Knoxville

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Antonín Bukovský

Creative Research Enterprises (United States)

Michael R. Caudle

Cherokee Nation

Roberto Domı́nguez

Universidad Nacional Autónoma de México

Gupta Sk

All India Institute of Medical Sciences

Nirmala B. Upadhyaya

University of Tennessee Medical Center

María E. Ayala

Universidad Nacional Autónoma de México

Irma Virant‐Klun

Ljubljana University Medical Centre

Rachel Selleck-White

University of Tennessee System

Stuart E. Van Meter

University of Tennessee at Knoxville

Pleas Copas

University of Tennessee at Knoxville

Cooperative Institutions

University of Tennessee at Knoxville

Alcoa (United States)

University of Tennessee Medical Center

Charles University

National Institute of Immunology

Universidad Nacional Autónoma de México

Czech Academy of Sciences

Czech Academy of Sciences, Institute of Biotechnology

General University Hospital in Prague

Prysmian Group (United States)

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Differential transferrin expression in placentae from normal and abnormal pregnancies: a pilot study

Reproductive Biology and Endocrinology (2008)

Alena Králová Marta Svetlikova J Madár Z Ulčová-Gallová Antonín Bukovský

The placenta is an important site for iron metabolism in humans. It transfers iron from the mother to the fetus. One of the major iron transport proteins is transferrin, which is a blood plasma protein crucial for iron uptake. Its localization and expression may be one of the markers to distinguish placental dysfunction.In the experimental study we used antibody preparation, mass spectrometric analysis, biochemical and immunocytochemical methods for characterization of transferrin expression on the human choriocarcinoma cell line JAR (JAR cells), placental lysates, and cryostat sections. Newly designed monoclonal antibody TRO-tf-01 to human transferrin was applied on human placentae from normal (n = 3) and abnormal (n = 9) pregnancies.Variations of transferrin expression were detected in villous syncytiotrophoblast, which is in direct contact with maternal blood. In placentae from normal pregnancies, the expression of transferrin in the syncytium was significantly lower (p < 0.001) when compared to placentae from abnormal ones (gestational diabetes, pregnancy induced hypertension, drug abuse).These observations suggest that in the case of abnormal pregnancies, the fetus may require higher levels of transferrin in order to prevent iron depletion due to the stress from the placental dysfunction.

Syncytiotrophoblasts

Syncytium

Transferrin receptor

Trophoblast

10.1186/1477-7827-6-27

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Citations (13)

Oogenesis in Adult Mammals, Including Humans: A Review

Endocrine (2005)

Antonín Bukovský Michael R. Caudle Marta Svetlikova Jay Wimalasena María E. Ayala

Ovarian Cortex

Follicular atresia

Tunica albuginea (penis)

10.1385/endo:26:3:301

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Citations (94)

Production of monoclonal antibodies against recombinant human zona pellucida glycoproteins: utility in immunolocalization of respective zona proteins in ovarian follicles

Journal of Reproductive Immunology (2008)

Antonín Bukovský Gupta Sk Pankaj Bansal S. C. Chakravarty Manjari Chaudhary

Antral follicle

Zona pellucida glycoprotein

10.1016/j.jri.2007.10.004

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Citations (27)

Study Origin of Germ Cells and Formation of New Primary Follicles in Adult Human and Rat Ovaries

Methods in molecular biology (2008)

Antonín Bukovský Gupta Sk Irma Virant‐Klun Nirmala B. Upadhyaya Pleas Copas

10.1007/978-1-60327-214-8_16

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Citations (32)

Ovarian Germ Cells

Methods in enzymology on CD-ROM/Methods in enzymology (2006)

Antonín Bukovský Irma Virant‐Klun Marta Svetlikova Isabelle Willson

Totipotent

Amniotic epithelial cells

10.1016/s0076-6879(06)19010-2

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Citations (16)

Bone Marrow Derived Cells and Alternative Pathways of Oogenesis in Adult Rodents

Cell Cycle (2007)

Antonín Bukovský María E. Ayala Roberto Domı́nguez Marta Svetlikova Rachel Selleck-White

Not yet available.

10.4161/cc.6.18.4707

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Citations (24)

Steroid-mediated differentiation of neural/neuronal cells from epithelial ovarian precursors in vitro

Cell Cycle (2008)

Antonín Bukovský Michael R. Caudle Marta Svetlikova

We reported earlier that occasional neurons evolve in human cultures of pluripotent ovarian epithelial stem cells. In subsequent experiments, frequent transdifferentiation into neural stem cells (NSC) and differentiating neurons was observed in human ovarian epithelial stem cells and porcine granulosa cells after exposure to certain combinations of sex steroids. Testosterone (TS), progesterone (PG) or estradiol (E2) alone do not increase the emergence of neurons. However, a mixture of TS+PG after E2 pretreatment converted a majority of ovarian epithelial stem cells or porcine granulosa cells into NSC and differentiating neuronal cells within one to three hours. Cultured neurons manifested an interconnectivity resembling primitive neuronal pathways in culture. These converted cells expressed the cell markers SSEA-1, SSEA-4, NCAM, and Thy-1 glycoconjugates of NSC and neurons, and differentiating cells showed characteristic neuronal morphology. Emergence of NSC and neuronal cells was associated with significant cellular depletion of L-glutamic acid (glutamate), which serves as the major excitatory neurotransmitter in the vertebrate CNS and its fast removal is essential for preventing glutamate excitotoxicity. These observations suggest that certain sequential systemic treatment with common sex steroids and their mixture might be effective in the treatment or prevention of degenerative CNS disorders. The ovarian stem cell cultures readily obtainable from human ovaries regardless of the woman's age have the potential to produce NSC for autologous regenerative treatment of neurologic diseases in aging women. Finally, the proper combination of sex steroids could possibly be employed for transdifferentiation of adult bone marrow stem cells or mobilized peripheral blood cells into autologous NSC and stimulate their neuronal differentiation after homing in the CNS.

10.4161/cc.7.22.7101

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Citations (29)

Oogenesis in cultures derived from adult human ovaries

Reproductive Biology and Endocrinology (2005)

Antonín Bukovský Marta Svetlikova Michael R. Caudle

Abstract Ten years ago, we reported that in adult human females the ovarian surface epithelium (OSE) is a source of germ cells. Recently, we also demonstrated that new primary follicles are formed by assembly of oocytes with nests of primitive granulosa cells in the ovarian cortex. The components of the new primary follicles, primitive granulosa and germ cells, differentiated sequentially from the OSE, which arises from cytokeratin positive mesenchymal progenitor cells residing in the ovarian tunica albuginea. In the present study, we investigated the possibility that the oocytes and granulosa cells may differentiate in cultures derived from adult human ovaries. Cells were scrapped from the surface of ovaries and cultured for 5 to 6 days, in the presence or absence of estrogenic stimuli [phenol red (PhR)]. The OSE cells cultured in the medium without PhR differentiated into small (15 micron) cells of granulosa phenotype, and epithelial, neural, and mesenchymal type cells. In contrast, OSE cells cultured in the presence of PhR differentiated directly into large (180 micron) cells of the oocyte phenotype. Such cells exhibited germinal vesicle breakdown, expulsion of the polar body, and surface expression of zona pellucida proteins, i.e. characteristics of secondary oocytes. These in vitro studies confirm our in vivo observations that in adult human ovaries, the OSE is a bipotent source of oocytes and granulosa cells. Development of numerous mature oocytes from adult ovarian stem cells in vitro offers new strategies for the egg preservation, IVF utilization, and treatment of female infertility. In addition, other clinical applications aiming to utilize stem cells, and basic stem cell research as well, may employ totipotent embryonic stem cells developing from fertilized oocytes.

Ovarian Cortex

Germinal vesicle

10.1186/1477-7827-3-17

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Citations (192)

[Extended embryo culture in IVF does not improve pregnancy rate].

PubMed (2011)

J Pavelková K Řežábek Martina Moosová Marta Svetlikova

Compare the effectiveness of the IVF extended embryo culture day 4 versus day 2.Retrospective analysis.Assisted Reproduction centre Apolinar, Department of Obstetrics and Gynecology, 1st Faculty of Medicine, Charles University and General Faculty Hospital in Prague.We compared pregnancy rate (PR) and implantation rate (IR) of all IVF and IVF/ICSI embryo transfers (ET) performed between August 2004 and July 2008, in which 2 embryos were transfered. All transfers were done by the same physician. Maternal age was < or = 35 years (from 22 to 35 years, mean 30.4 years, median 31.1 years). The group of embryos evaluated and transfered on the day 2 was compared with the embryos evaluated and transfered on the day 4. Based on the evaluation, each embryo was assigned to one of the following categories: Grade A (top quality embryo) or grade B (medium quality embryo).On the day 2, ET of A+A embryos led to PR 69.0%; A+B 53.3% and B+B 36.4% (IR 50.0%, 40.0% and 22.7%), where number of transfers was 42, 30 and 11 in every group. On the day 4, ET of A+A embryos led to PR 54.3%, A+B 48.7%, B+B 26.6% (IR 45.6%, 34.6%, 13.3%), where number of transfers was 46.39 and 15 in every group.In our group, we found no statistical significant difference (p < 0.05) in PR between day 2 and day 4 embryotransfers. Results of the day 2 transfers have a trend to be better than results of day 4 transfers. Regardless of the transfer day, the prognosis of grade A embryos was significantly better (p < 0.05) than for grade B embryos.

Group B

Embryo quality

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Citations (1)

Mammalian neo-oogenesis and expression of meiosis-specific protein SCP3 in adult human and monkey ovaries

Cell Cycle (2008)

Antonín Bukovský Michael R. Caudle Gupta Sk Marta Svetlikova Rachel Selleck-White

Not yet available.

10.4161/cc.7.5.5453

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Citations (35)