Hironori Ishida

Saitama Medical University

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Hirozo Sakaguchi

Saitama Medical University

Hiroyuki Nitanda

Saitama Medical University

Ryo Taguchi

Kyushu University Hospital

Akitoshi Yanagihara

Saitama Medical University

Tetsuya Umesaki

Saitama Medical University

Hiroshi Kagamu

Saitama Medical University

Kyoichi Kaira

Saitama Medical University

Kunihiko Kobayashi

Saitama Medical University

Hisao Imai

Saitama Medical University

Katsuhisa Horimoto

National Institute of Advanced Industrial Science and Technology

Cooperative Institutions

Saitama Medical University

Kyoto University

The University of Tokyo

Osaka University

Asahikawa Medical College Hospital

Kitasato University

Tohoku University

Saitama International Medical Center

Kyushu University

Tokyo Women's Medical University

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Supplementary Figure S4 from A Th1-like CD4+ T-cell Cluster that Predicts Disease-free Survival in Early-Stage Lung Cancer

Akitoshi Yanagihara Satoshi Yamasaki Kosuke Hashimoto Ryo Taguchi Tetsuya Umesaki

Fig. S4. The percentages of CD8+ T-cell clusters in the peripheral blood were compared before and after surgical resection in patients with no recurrence and patients with recurrence. Differences were tested by paired Welch's t-test

10.1158/2767-9764.23600233

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Table S1, Table S2 from A Th1-like CD4+ T-cell Cluster that Predicts Disease-free Survival in Early-Stage Lung Cancer

Akitoshi Yanagihara Satoshi Yamasaki Kosuke Hashimoto Ryo Taguchi Tetsuya Umesaki

Table S1. List of antibodies used for CyTOF. Table S2. Differentially expressed genes between Th7R cluster and Th1 cluster in tumor-infiltrating lymphocytes.

Table (database)

10.1158/2767-9764.23600230

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Table S1, Table S2 from A Th1-like CD4+ T-cell Cluster That Predicts Disease-free Survival in Early-stage Lung Cancer

Akitoshi Yanagihara Satoshi Yamasaki Kosuke Hashimoto Ryo Taguchi Tetsuya Umesaki

Table S1. List of antibodies used for CyTOF.Table S2. Differentially expressed genes between Th7R cluster and Th1 cluster in tumor-infiltrating lymphocytes.

Table (database)

10.1158/2767-9764.23709851.v1

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The prognostic significance of p53 andbcl-2 expression in lung adenocarcinoma and its correlation with ki-67 growth fraction

Cancer (1997)

Hironori Ishida Koji Irie Tsuyoshi Itoh T Furukawa Osamu Tokunaga

The p53 and bcl-2 gene deregulations are frequently involved in several types of epithelial malignancy. The aims of this study were to determine the relationships between the Ki-67 labeling index (LI) and the expression of p53 and bcl-2 protein in lung adenocarcinoma and to investigate the clinicopathologic features of the tumors and the prognostic significance of p53 and bcl-2.Immunohistochemical staining for p53, bcl-2, and Ki-67 was performed on paraffin embedded sections from 114 lung adenocarcinomas from patients who had been treated with complete and potentially curative surgery. To define a cutoff value for p53 immunopositivity, 37 of 114 tumors were examined for p53 gene mutations using cDNA sequencing. The results were compared with clinical outcomes.Expression of p53 protein was defined as nuclear staining in more than 20% of tumor cells, and the concordance rate between p53 expression and gene mutation was 73%. Expression of p53 (39%) was significantly associated with higher Ki-67 LI (P < 0.001) and with several poor prognostic factors, such as poor histologic grade of differentiation (P < 0.05), lymphatic permeation (P < 0.001), and vascular permeation (P < 0.001). Expression of bcl-2 (38%) was significantly associated with favorable T classification (P < 0.001) and smaller tumor size (<2 cm) (P < 0.05) and tended to be associated with lower Ki-67 LI (P = 0.054). In univariate analysis, p53 expression was significantly correlated with poor prognosis (53% vs. 90% at 5 years, P < 0.001) and bcl-2 expression with favorable prognosis (90% vs. 68% at 5 years, P < 0.05). Proliferative activity was highest when only p53 was expressed; by contrast, proliferative activity was lowest when only bcl-2 was expressed. Among four groups of patients, the group that was positive only for p53 had the shortest survival; this difference was statistically significant. Multivariate analysis showed that T classification (P = 0.002), vascular permeation (P = 0.011), and p53 expression (P = 0.03) were independent prognostic factors.The results of this study indicated an inverse relationship between the proliferative activity of p53 and bcl-2. Expression of p53 was an independent factor associated with poor prognosis, whereas bcl-2 expression was associated with favorable prognosis.

Ki-67

Clinical Significance

Univariate analysis

10.1002/(sici)1097-0142(19970915)80:6<1034::aid-cncr5>3.0.co;2-9

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Citations (100)

[Pulmonary resection for tuberculosis--report of 1134 cases].

PubMed (1968)

Ryota Sato Hironori Ishida Shigeo Soda Y Matsumoto

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Kidney Disease Quality of Life of Japanese Dialysis Patients Who Desire Administration of Sildenafil and the Treatment of Erectile Dysfunction Using Sildenafil

Therapeutic Apheresis and Dialysis (2004)

Toru Hyodo Hironori Ishida Noriaki Masui Takayasu Taira Sumiko Yamamoto

Abstract: Erectile dysfunction (ED) is common among patients on dialysis therapy. In the present study, we attempted administration of sildenafil to Japanese patients undergoing dialysis. In order to diagnose ED before prescribing sildenafil, we assessed the hemodialysis patients who desired sildenafil by using the five items version of the International Index of Erectile Function (IIEF‐5). In addition, the characteristics of the quality of life in Japanese hemodialysis patients who desired sildenafil were assessed using the kidney disease quality of life (KDQOL). To all 37 male subjects (mean age of 53.8 ± 10.4 years) attending the Outpatient Hemodialysis Unit at Atsugi Clinic (Atsugi City, Japan), it was explained by their primary doctor that the treatment of ED with sildenafil was possible. As a result, 10 patients (27.0%) desired the treatment. For eight patients, ED was diagnosed by IIEF‐5 prior to prescription of sildenafil. The mean IIEF‐5 scores were 6.13 ± 4.67 points. Sildenafil was prescribed to five patients (three diabetic, two non‐diabetic) and sexual function was improved in three cases. The main adverse effect was found to be ventricular arrhythmia in one case. As for KDQOL, the group desiring sildenafil showed significantly high values in Dialysis Staff Encouragement and Patient Satisfaction. Among the other nine dialysis patients (five diabetic, four non‐diabetic; mean age of 58.1 ± 8.9 years) who visited the ED department of Ishida Hospital (Asahikawa City, Japan), sildenafil was effective for all non‐diabetic patients (100%) and for only one diabetic patient (20%). Among all 14 patients at Atsugi Clinic and Ishida Hospital, sildenafil efficacy rates were 83.3% for non‐diabetic patients and 37.5% for diabetic patients. Non‐diabetic patients without the side‐effects were all responders for the sildenafil treatment. The patients who relied on the dialysis staff and were more satisfied with the general treatment in the dialysis institute desired the administration of sildenafil under the present circumstances wherein the dialysis population had few experiences of sildenafil treatment. Diabetic status is thought to be a negative factor for the response of sildenafil treatment in dialysis patients.

Outpatient clinic

10.1111/j.1526-0968.2004.00163.x

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Multidisciplinary treatment of pancreatic cancer with major arterial involvement in the US

HPB (2024)

Oskar Franklin Thomas F. Stoop Toshitaka Sugawara Salvador Rodriguez Franco Hironori Ishida

10.1016/j.hpb.2024.04.358

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IMMUNOHISTOCHEMICAL STUDY WITH MONOCLONAL ANTIBODY AGAINST ^|^gamma;-SEMINOPROTEIN IN THE PROSTATIC TISSUES

The Japanese Journal of Urology (1993)

Akito Fujino Hironori Ishida M Ohhori Shigehiro Soh K Nishimura

The tissues consisted of adenocarcinoma of the prostate were examined by immunohistochemical study with monoclonal antibody (43-21-1-1) against γ-seminoprotein (γSm). The degree of staining regarding any correlation with the histological grade was evaluated. The prostatic tissues were obtained by transurethral resection or by fine needle biopsy from untreated 38 patients. The avidinbiotin peroxidase complex technique was used to stain on 3μm-sections of 10% formalin fixed, paraffin embedded tissue. In addition, immunohistochemical staining with the commercialized antibodies to prostate-specific antigen (PSA; polyclonal, DAKO) and to prostatic acid phosphatase (PAP; polyclonal, DAKO) was done simultaneously for a comparative study. The degree of immunoperoxidase stain was classified into two categories, namely location and pattern, and was graded from 0 to 3, respectively. The product of the degree of location and the degree of pattern was noted as the total score. The mean of score was calculated in each histological grade. Then the means of total scores were compared and evaluated as having any statistical difference by Student's t test among 3 histological grades as well as among 3 primary antibodies used in this study.When the monoclonal antibody to γ-Sm was used for immunoperoxidase staining, the means of total scores and the rates of negative reactions (% Negative) in 3 histological grades were 6.8±1.8 (M±SD) and 0% in well (N=9), 4.4±2.4 and 14% in moderately (N=22), and 1.8±2.3 and 54% in poorly differentiated lesions (N=11), respectively. There were statistically significant differences (P<0.05) among 3 histological grades. The means of total scores and % Negative by PAP staining were 7.1±1.5 and 0% in well (N=8), 6.1±2.5 and 9% in moderately (N=22), and 4.3±3.2 and 30% in poorly differentiated lesions (N=10) respectively. However, no statistical differences were noted among 3 histological grades. The PSA staining demonstrated almost the same findings as did PAP staining. The means of total scores and % Negative by PSA staining were 7.1±1.5 and 0% in well (N=8), 5.9±2.5 and 9% in moderately (N=21), and 3.8±2.5 and 10% in poorly differentiated lesions (N=10), respectively. There was no statistical difference between the moderate and the poor, but between well and moderately differentiated lesions (p<0.05).In conclusion, the degree of immunoperoxidase staining with the monoclonal antibody to γ-Sm correlated well with histological grade. The monoclonal antibody to γ-Sm may have an important role to distinguish between moderately and poorly differentiated adenocarcinoma of the prostate.

Immunoperoxidase

Stain

Antigen retrieval

Polyclonal antibodies

10.5980/jpnjurol1989.84.720

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Report of macroamylasemia in a hemodialysis patient suffering from chronic renal failure due to Sjoegren syndrome.

Nihon Toseki Igakkai Zasshi (1996)

S Yachiku Yasuhiro Nakamura Tsutomu Anzai Takeshi Kobayashi Hironori Ishida

患者は66歳の女性で, Sjögren症候群による腎不全に対して, 1992年1月から血液透析中であった. 1994年7月, 両側耳下腺部の腫脹, 頸部リンパ節腫脹, 発熱, 羞明などの症状とともに, 血清アミラーゼ値が1, 195IU/lと異常高値を示した. 電気泳動法によりアミラーゼのアイソザイム分析を行ったところ, 分画不明な異常パターンを呈した. そこで, Superose 12ゲル濾過HPLC, 免疫混合法, 免疫電気向流法で精査したところ, このアミラーゼは, Ig-Aλ型との結合によるマクロアミラーゼであり, これによるアミラーゼの高値であることが判明した.本症例は頻回の消化管出血があり, 繰り返し精査を行ったが, その原因を確定することはできなかった. 試験開腹の際に行われたリンパ節生検の組織診により非ホジキン型の悪性リンパ腫の合併が判明した. しかし術後DICを併発し, 不幸な転機をとった.血液透析患者では血清アミラーゼ値は正常人よりもその平均が高値である. また膵炎などにより, さらに高値になることは経験されるが, 透析患者のマクロアミラーゼ血症合併の報告は極めて少なく, またSjögren症候群との合併の報告も見当たらず, 稀な症例と考えられる.なおこの症例におけるマクロアミラーゼ血症の発生に関して, 文献的に考察を加えた.

Chronic renal failure

10.4009/jsdt.29.69

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Original Articles p53 MUTATIONS AND PROGNOSIS IN BLADDER TUMORS

Τοyoaki Uchida Chieki Wada Hironori Ishida Handong Wang Shinichi Egawa

The incidence of loss of heterozygosity on chromosome 17p and p53 gene mutations was assessed in 43 bladder tumor patients. Histological findings, cigarette smoking and prognosis were examined for possible correlation with the presence or absence of loss of heterozygosity on 17p and p53 mutations. Of 20 informative cases 10 (50.0%) showed loss of heterozygosity of 17~13, including 9 (90.0%) with disease beyond stage pT2. The p53 mutations were detected in 20 of 43 patients (46.5%), including 9 (95.0%) with disease beyond grade 2 and 17 (85.0%) with cancer beyond stage pT2. The incidence of p53 gene mutations was not significantly influenced by habitual smoking but G:C to T:A substitutions, often observed in lung cancers, were detected only in mutations from smokers (5 of 10 or 50%, p <0.05). Groups with and without loss of heterozygosity showed essentially the same results, while significant differences were found for groups with grades 1 and 2 to 3 (p <0.05) cancer, stages pT1 and pT2 to 4 (p ~0.01) disease, and with and without p53 gene mutations (p <0.01, Cox-Mantel test). Genetic alternation in chromosome 17p and p53 mutations would, thus, appear to occur more frequently in high grade and invasive bladder tumors. Cigarette smoking may possibly be a determining factor of mutations of the p53 gene in bladder tumors. Our results indicate that an unfavorable prognostic factor may possibly be linked not only to histopathological findings but the presence of a p53 mutation in bladder tumors as well. Accordingly, mutations of the p53 gene may be deeply involved in late events of tumorigenesis and possibly useful as ideal molecular markers for prognosis in bladder tumors. Bladder cancer is the fifth and fourteenth most common chromosome 17p, as indicated by analysis with restriction tumor in the United States and Japan, respectively, with an fragment length polymorphism, frequently has been obannual incidence of approximately 18 and 4.8 cases per served in grades 3 and 4 tumor^.^.^ me frequency of p53 100,000 population.'s2 Approximately 80% of bladder cancer mutations in primary bladder cancers is determined directly patients present with superficial tumors that often recur, in only a limited number of cases and these mutations fieand require careful observation and clinical fOllOWUP. InVa- quently have been observed in primary invasive bladder tusive tumors are more likely to progress and patients are at mors.10-12 N~ information presently is on the proghigh risk of dying despite radical surgery, radiation and nosis of bladder tumors in relation to cbomosome 17~ delic ~hemotherapy.~ loss and p53 gene mutation. defects in tumors remain Recently, the mutation patterns of the p53 gene have been unclear but results obtained using advanced techniques in shown to refled the specific carcinogen(s) involved.l3 For examthe analysis of other cancers indicate that cumulative genetic ple, lung closely associated with cigarette smoking often alterations occur during tumor development. These alterations involve the activation of oncogenes and inactivation of 'Onbin a GC to Of mutation 's pre

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