Yanxiu Ni

Jiangsu Academy of Agricultural Sciences

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Kongwang He

Yangzhou University

Junming Zhou

Jiangsu Academy of Agricultural Sciences

Xuehan Zhang

Peking Union Medical College Hospital

Zhengyu Yu

Sichuan University

Libin Wen

Ministry of Agriculture and Rural Affairs

Aihua Mao

Shantou University

Lixin Lv

Tongji Hospital

Rongli Guo

Jiangsu Academy of Agricultural Sciences

Bin Li

Jiangsu Academy of Agricultural Sciences

Haodan Zhu

Jiangsu Academy of Agricultural Sciences

Cooperative Institutions

Jiangsu Academy of Agricultural Sciences

Yangzhou University

Nanjing Agricultural University

Ministry of Agriculture and Rural Affairs

Jiangsu University

State Key Laboratory of Food Science and Technology

Chinese Academy of Medical Sciences & Peking Union Medical College

Ministry of Science ICT and Future Planning

Ministère de l'Agriculture et de la Souveraineté alimentaire

Central South University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

The Redox-Sensing Regulator Rex Contributes to the Virulence and Oxidative Stress Response of Streptococcus suis Serotype 2

Frontiers in Cellular and Infection Microbiology (2018)

Haodan Zhu Yong Wang Yanxiu Ni Junming Zhou Lixiao Han

Streptococcus suis serotype 2 (SS2) is an important zoonotic pathogen responsible for septicemia and meningitis. The redox-sensing regulator Rex has been reported to play critical roles in the metabolism regulation, oxidative stress response, and virulence of various pathogens. In this study, we identified and characterized a Rex ortholog in the SS2 virulent strain SS2-1 that is involved in bacterial pathogenicity and stress environment susceptibility. Our data show that the Rex-knockout mutant strain Δrex exhibited impaired growth in medium with hydrogen peroxide or a low pH compared with the wildtype strain SS2-1 and the complementary strain CΔrex. In addition, Δrex showed a decreased level of survival in whole blood and in RAW264.7 macrophages. Further analyses revealed that Rex deficiency significantly attenuated bacterial virulence in an animal model. A comparative proteome analysis found that the expression levels of several proteins involved in virulence and oxidative stress were significantly different in Δrex compared with SS2-1. Electrophoretic mobility shift assays revealed that recombinant Rex specifically bound to the promoters of target genes in a manner that was modulated by NADH and NAD+. Taken together, our data suggest that Rex plays critical roles in the virulence and oxidative stress response of SS2.

Streptococcus suis

10.3389/fcimb.2018.00317

Cite

Citations (16)

Comprehensive analysis of codon usage patterns of porcine deltacoronavirus and its host adaptability

Transboundary and Emerging Diseases (2022)

Qi Peng Xue Zhang Jizong Li Wenlong He Baochao Fan

The porcine deltacoronavirus (PDCoV) is a newly discovered pig enteric coronavirus that can infect cells from various species. In Haiti, PDCoV infections in children with acute undifferentiated febrile fever were recently reported. Considering the great potential of inter-species transmission of PDCoV, we performed a comprehensive analysis of codon usage patterns and host adaptation profiles of 54 representative PDCoV strains with the spike (S) gene. Phylogenetic analysis of the PDCoV S gene indicates that the PDCoV strains can be divided into five genogroups. We found a certain codon usage bias existed in the S gene, in which the synonymous codons are often ended with U or A. Heat map analysis revealed that all the PDCoV strains shared a similar codon usage trend. The PDCoV S gene with a dN/dS ratio lower than 1 reveals a negative selection on the PDCoV S gene. Neutrality analysis showed that natural selection is the dominant force in shaping the codon usage bias of the PDCoV S gene. Unexpectedly, host adaptation analysis reveals a higher adaptation level of PDCoV to Homo sapiens and Gallus gallus than to Sus scrofa. Compared to the USA lineage, the PDCoV strains in the Early China lineage and Thailand lineage were less adapted to their hosts, which indicates that the evolutionary process plays an important role in the adaptation ability of PDCoV. These findings of this study add to our understanding of PDCoV's evolution, adaptability, and inter-species transmission.

Lineage (genetic)

Host adaptation

Coronavirus

10.1111/tbed.14588

Cite

Citations (12)

In vitro and In vivo Antibacterial Effects of Nisin Against Streptococcus suis

Probiotics and Antimicrobial Proteins (2021)

Haodan Zhu Lixiao Han Yanxiu Ni Zhengyu Yu Dandan Wang

Streptococcus suis

Minimum bactericidal concentration

10.1007/s12602-020-09732-w

Cite

Citations (15)

Complete Genome Sequence of Streptococcus suis Serotype 2 Virulent Strain SS2-1

Genome Announcements (2018)

Haodan Zhu Yanxiu Ni Junming Zhou Zhengyu Yu Aihua Mao

Streptococcus suis is an important swine pathogen that can also cause severe diseases in humans. Herein, we describe the genome sequence of Streptococcus suis serotype 2 virulent strain SS2-1, which was isolated from a diseased dead pig amid the 1998 Streptococcus suis outbreak in Jiangsu Province in China.

Streptococcus suis

Strain (injury)

10.1128/genomea.00067-18

Cite

Citations (1)

Detection of Transmissible Gastroenteritis Virus (TGEV) by Reverse Transcription-Polymerase Chain Reaction

Zhongguo shouyi xuebao (2001)

Jun Li Lin Ji-Huang LU Cheng-ping Kongwang He Yanxiu Ni

A pair of oigonucleotide primers appropriate for PCR amplication were selected based on the two conserved regions of the 5^ end of the gene encoding the S protein of TGEV. A reverse transcription-polymerase chain reaction (RT-PCR) assay was developed for the detection of TGEV after optimization of the reaction conditions. The sandwich ELISA was also used for control. Out of 56 fecal samples, 20 were positive detected by RT-PCR, meanwhile only 14 were positive detected by sandwich ELISA. The corresponding rate of the two methods was 89%. It suggests that RT-PCR is more sensitive than sandwich ELISA and should be quite useful for detection of TGEV and the epidemiiological survey of TGE.

Source

Cite

Citations (0)

Genome Sequence of a Highly Prevalent Porcine Partetravirus in Mainland China

Journal of Virology (2012)

Bin Li Junjie Ma Shaobo Xiao Libin Wen Yanxiu Ni

ABSTRACT Partetravirus is a novel defined genus of animal parvoviruses. Here, we first report the genome sequence of porcine partetravirus strain JSNJ62, which is highly prevalent in mainland China. It will help in understanding the epidemiology and molecular characteristics of the porcine partetravirus.

Sequence (biology)

Strain (injury)

10.1128/jvi.06771-11

Cite

Citations (13)

Contribution of Eukaryotic-Type Serine/Threonine Kinase to Stress Response and Virulence of Streptococcus suis

PLoS ONE (2014)

Haodan Zhu Junming Zhou Yanxiu Ni Zhengyu Yu Aihua Mao

Streptococcus suis serotype 2 (SS2) is an important swine and human pathogen responsible for septicemia and meningitis. The bacterial homologues of eukaryotic-type serine/threonine kinases (ESTKs) have been reported to play critical roles in various cellular processes. To investigate the role of STK in SS2, an isogenic stk mutant strain (Δstk) and a complemented strain (CΔstk) were constructed. The Δstk showed a significant decrease in adherence to HEp-2 cells, compared with the wild-type strain, and a reduced survival ratio in whole blood. In addition, the Δstk exhibited a notable reduced tolerance of environmental stresses including high temperature, acidic pH, oxidative stress, and high osmolarity. More importantly, the Δstk was attenuated in both the CD1 mouse and piglet models of infection. The results of quantitative reverse transcription-PCR (qRT-PCR) analysis indicated that the expressions of a few genes involving in adherence, stress response and virulence were clearly decreased in the Δstk mutant strain. Our data suggest that SsSTK is required for virulence and stress response in SS2.

Streptococcus suis

Wild type

10.1371/journal.pone.0091971

Cite

Citations (31)

Co‐infection analysis of bacterial and viral respiratory pathogens from clinically healthy swine in Eastern China

Veterinary Medicine and Science (2021)

Haodan Zhu Xinjian Chang Jinzhu Zhou Dandan Wang Junming Zhou

Abstract Porcine respiratory disease complex (PRDC) is one of the most challenging health concerns for pig production worldwide. The aim of the present study was to determine the prevalence of pathogens associated with PRDC, including porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) and bacterial agents, such as Streptococcus suis , Haemophilus parasuis and Actinobacillus pleuropneumoniae , in clinically healthy pigs in Eastern China. Molecular detection revealed positive single‐pathogen detection rates of 59.9%, 27.2%, 52.3%, 33.2% and 0.4% for PCV2, PRRSV, S . suis , H . parasuis and A . pleuropneumoniae , respectively. Co‐infection with more than one pathogen was frequently detected in these samples, with PCV2/ S . suis , H . parasuis and PCV2/ H . parasuis mixed infection rates of 35.4%, 33.2% and 21.6%, respectively, and PCV2/ S . suis / H . parasuis and PRRSV/PCV2/ S . suis co‐infection rates of 21.6% and 6.2%, respectively. These results suggest that mixed infections are prevalent among PRDC cases in swine, which may pose a greater threat to the health of herds compared with single‐pathogen infections.

Streptococcus suis

Actinobacillus pleuropneumoniae

Circovirus

10.1002/vms3.533

Cite

Citations (24)

Development of a loop-mediated isothermal amplification assay for the detection of porcine hokovirus

Journal of Virological Methods (2013)

Bin Li Bing Sun Lu-ping Du Aihua Mao Libin Wen

Porcine parvovirus

Pseudorabies

Agarose gel electrophoresis