M. Saeed Arayne

University of Karachi

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Najma Sultana

University of Karachi

138

Farhan Ahmed Siddiqui

University of Karachi

Agha Zeeshan Mirza

University of Karachi

Safila Naveed

University of Karachi

Urooj Haroon

Quaid-i-Azam University

Sana Shamim

Dow University of Health Sciences

Muhammad Nawaz

Imam Abdulrahman Bin Faisal University

M. Hashim Zuberi

Sindh Madressatul Islam University

Somia Gul

Jinnah University for Women

Faiza Qureshi

Aga Khan University

Cooperative Institutions

University of Karachi

Federal Urdu University

Dow University of Health Sciences

International Center for Chemical and Biological Sciences

University of the Punjab

Jinnah University for Women

University of Calgary

Bahauddin Zakariya University

Government College University, Faisalabad

Government College University, Lahore

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Concurrent Determination of Diltiazem, Lisinopril, Captopril, and Enalapril in Dosage Formulations and in Human Serum by Liquid Chromatographic Technique

Journal of Liquid Chromatography &amp Related Technologies (2015)

Nighat Shafi Farhan Ahmed Siddiqui Najma Sultana M. Saeed Arayne

AbstractThe aim of this study was to develop an analytical method that can quantify diltiazem, lisinopril, captropil, and enalapril in pharmaceuticals dosage forms and biological fluids, as these drugs are co-administered drugs. For the determination of diltiazem and ACE inhibitors a novel, trouble-free, quick, isocratic high performance liquid chromatography method has been established. The ACE inhibitors, which were assayed alongside diltiazem, include lisinopril, captopril, and enalapril. These drugs were tested in bulk, dosage formulations, and human serum. The analytes were separated on Pruospher Star RP-18 endcapped (250 mm × 4.6 mm id), and the mobile phase composition was acetonitrile:methanol:water (5:45:50 v/v/v). The pH of the mobile phase was adjusted with ortho-phosphoric acid at 2.5. The flow rate was maintained at 1 mLmin−1 at room temperature (25°C). The maximum wavelength for the assay was 230 nm on a UV detector. ICH validation parameters were followed while validating the method. The selected concentration range for establishing the linearity for diltiazem was 2.5–25 µgmL−1, 20–200 µgmL−1 for lisinopril enalapril, and 30–300 µgmL−1 for captopril. This linearity range was best as was demonstrated by r2 ≥ 0.999 (n = 6).Keywords: captoprildiltiazemdosage formenalaprilhuman serumlisinoprilRP-HPLC

Lisinopril

Captopril

10.1080/10826076.2015.1050503

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Citations (14)

High‐Performance Liquid Chromatography Assay for Moxifloxacin in Bulk, Pharmaceutical Formulations and Serum: Application to In‐Vitro Metal Interactions

Journal of the Chinese Chemical Society (2010)

Najma Sultana M. Saeed Arayne Mahwish Akhtar Sana Shamim Somia Gul

Abstract A simple reversed phase HPLC method have been successfully developed and validated for the quantitative determination of moxifloxacin (MOX) in bulk material, pharmaceutical formulation and serum. Purospher STAR C 18 (25 cm × 4.6 mm, 5 μm) and Discovery C 18 (25 cm × 4.6 mm, 5 μm) columns were used. The mobile phase, methanol: acetonitrile: water (85:5:10, v/v/v), pH 2.75 adjusted by phosphoric acid was delivered at a flow rate of 1 mL min −1 . The eluents was monitored using UV detector at 290 nm. The proposed method is specific, accurate (99.39‐102.67%), precise (intra‐day variation 0.04‐0.86% and inter‐day variation 0.12‐0.94%) and linearity (R 2 > 0.999) within the desired range 0.39‐25 μg mL −1 concentration. The detection and quantification limit was 0.002‐0.51 μg mL −1 and 0.01‐0.555 μg mL −1 , respectively. The analysis of variance (ANOVA) and student's t ‐test were applied to verify the results. The anticipated method is applicable to routine analysis of MOX in pharmaceutical formulations and human serum samples. It is also applied on interaction of MOX with several essential and trace metals as well as interaction with antacids.

Phosphoric acid

Pharmaceutical formulation

Coefficient of variation

10.1002/jccs.201000099

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Citations (34)

Sparfloxacin determination in bulk materials, pharmaceutical formulations and human serum by reverse phase HPLC method

African Journal of Pharmacy and Pharmacology (2013)

Somia Gul Najma Sultana M. Saeed Arayne Sana Shamim Mahwish Akhtar

A simple reverse phase high-performance liquid chromatography (HPLC) method was developed and validated for the quantitative determination of sparfloxacin (SPFX) in the bulk material, pharmaceutical formulation and human serum. Purospher Start C18 (25 cm × 4.6 mm, 5 µm) and Discovery C18 (25 cm × 4.6 mm, 5 µm) columns were used. The mobile phase, methanol, water and acetonitril (60:30:10 v/v/v pH 2.70 adjusted by phosphoric acid), was delivered at a flow rate of 1.0 mL min-1, eluent was monitored by ultra-violet (UV) detector at 290 nm. Gemifloxacin (GFX) was used as an internal standard. The proposed method is specific, accurate (98.11 to 102.83%), precise (intra and inter-day variations were 0.108 to 0.712% and 0.013 to 0.575%) and linearity was within the desired ranges of 2.5 to 100 µg mL-1 concentration having r2 > 0.998. All the results were correlated through analysis of variance (ANOVA) and Student’s t-test. The limit of detection (LOD) and limit of quantification (LOQ) were 0.0009 to 0.0064 and 0.0028-0.0196 µg mL-1, respectively. This method is not only applicable to routine analysis of SPFX in bulk and pharmaceutical formulations but as well as on human serum samples. Key words: Sparfloxacin, reverse phase high-performance liquid chromatography (RP-HPLC), serum, analysis of variance (ANOVA), student’s t-test.

Sparfloxacin

Phosphoric acid

10.5897/ajpp11.524

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Citations (1)

Simultaneous Determination of Cetirizine, Atorvastatin, Simvastatin, Rosuvastatin or Pravastatin in Formulations and Human Serum by RP-HPLC

Analytical Chemistry Letters (2015)

Hina Shamshad Najma Sultana M. Saeed Arayne

Present work was designed for the simultaneous determination of cetirizine in presence of atorvastatin, simvastatin, rosuvastatin or pravastatin using RP-HPLC method. The chromatographic separation achieved using mobile phase consisted of acetonitrile: water (75:25), monitored at 235 nm with a pH adjusted to 2.8. The linearity was assessed over the range of 2.5-100 µgmL-1 for all drugs. The applicability of the method was verified by the determination of cetirizine, atorvastatin, simvastatin, rosuvastatin and pravastatin in marketed formulations. The results of the method were validated statistically according to ICH guidelines. The recovery values for all the drugs in formulations were found to be 96-108 %. The parameters such as accuracy, precision, linearity, sensitivity (LOQ=0.01-0.07, LOD=0.003-0.02 µgmL-1) were found to be satisfactory. This method can be successfully used for the determination of these drugs in formulations and can be a useful tool for the routine analysis in laboratories.

Cetirizine

10.1080/22297928.2015.1062419

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Carboxyterfenadine antacid interaction monitoring by UV spectrophotometry and RP-HPLC techniques

Arabian Journal of Chemistry (2013)

Hina Shehnaz Amir Haider M. Saeed Arayne Najma Sultana

Carboxyterfenadine, a primary metabolite of terfenadine, a second generation antihistaminic compound was introduced in therapy as a successor of terfenadine due to its cardiac arrhythmia. There are number of drug interactions of fexofenadine with erythromycin, ketoconazole and alike reported in the literature. In this paper, fexofenadine antacid interaction has been studied in presence of sodium bicarbonate, megaldrate, calcium carbonate, magnesium carbonate, aluminum hydroxide, magnesium hydroxide, magnesium trisilicate, simethicone (dimethylpolysiloxane) and calcium hydroxide by UV–Vis spectrophotometer and high performance liquid chromatography (HPLC). These in vitro fexofenadine–antacid interactions were carried out in simulated gastric and intestinal juices and in buffer of pH 7.4 (simulating blood pH) on BP 2005 dissolution apparatus. The results show non-concordant availability of fexofenadine envisaged due to formation of unstable charge transfer complexes.

Antacid

Fexofenadine

Terfenadine

Spectrophotometry

10.1016/j.arabjc.2013.01.011

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Citations (3)

Determination of Tryptophan in Raw Materials, Rat Brain and Human Plasma by RP-HPLC Technique

Journal of Chromatographic Science (2012)

Najma Sultana M. Saeed Arayne Md Maruf Khan D. Saleem Agha Zeeshan Mirza

This paper describes tryptophan (TRP) estimation in raw human plasma and rat brain by reversed-phase high-performance liquid chromatography (RP-HPLC). Estimation was carried out on a Purospher STAR C18 column using water-acetonitrile (90:10 v/v, at pH 2.7) mixture at a rate of 1.5 mL/min as mobile phase. Eluents were monitored at 273 nm by an ultraviolet detector. The method was linear (R(2) > 0.999), precise (intra-day and inter-day precision <2%) in the range of 0.25-20 µg/mL. The detection and quantification limits were 0.0144 µg/mL and 0.0437 µg/mL, respectively. In human plasma, Day 1 and Day 2 precision were 0.054-2.29% and 1.66-3.7%; whereas precisions in rat brain were 1.23-2.3% and 0.677-4.2%, respectively. The method was applied to study TRP level in human smokers and in arthritic rat brain. An efficient RP-HPLC method was developed for TRP determination that worked for clinical and research purposes.

10.1093/chromsci/bms045

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Citations (22)

Novel derivatives of 5-amino-1-cyclopropyl-7-[(3R,5S)3,5-dimethylpiperazine-1-yl]-6,8-difluoro-4-oxo-quinoline-3-carboxylic acid: their synthesis, antimicrobial, antifungal, and urease inhibitory studies

Medicinal Chemistry Research (2013)

M. Saeed Arayne Najma Sultana Somia Gul Ajmal Khan

Quinoline

Amide

Quinolone

10.1007/s00044-013-0699-9

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Citations (9)

Simultaneous Determination of Gliquidone, Fexofenadine, Buclizine, and Levocetirizine in Dosage Formulation and Human Serum by RP-HPLC

Journal of Chromatographic Science (2010)

M. Saeed Arayne Nasrin Sultana M. Agha Zeeshan Farhan Ahmed Siddiqui

In the present paper, a simultaneous method has been developed and validated for estimation of gliquidone in the presence of H1- receptor antagonists (fexofenadine hydrochloride, buclizine hydrochloride, and levocetirizine dihydrochloride) using reversedphase high-performance liquid chromatographic technique. A good chromatographic separation between these drugs was achieved using a mobile phase containing methanol-water (80:20 v/v) at pH 3.5 with a flow rate of 1.0 mL/min; and detection was performed at 230 nm with a UV detector. Validation of the method was performed in terms of linearity, accuracy, precision, and limit of detection and quantification. The linearity of the calibration curves for gliquidone, fexofenadine hydrochloride, buclizine hydrochloride, and levocetirizine dihydrochloride were found to be 0.338-50 μg/mL (r = 0.9964), 5-50 μg/mL (r = 0.9956), 0.325-50 μg/mL (r = 0.9967), and 0.553-50 μg/mL (r = 0.9950), respectively. There was no significant difference between the amount of drug spiked in serum and the amount recovered, and serum did not interfere in simultaneous estimation. Thus, the proposed method is suitable for the simultaneous analysis of active ingredients in tablet dosage forms and human serum.

Fexofenadine

Levocetirizine

10.1093/chromsci/48.5.382

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Citations (32)

Validated method for the determination of Gemifloxacin in bulk, pharmaceutical formulations and human serum by RP-HPLC: in vitro applications

Journal of the Brazilian Chemical Society (2011)

Najma Sultana M. Saeed Arayne Sana Shamim Mahwish Akhtar Somia Gul

An isocratic reversed phase high-performance liquid chromatographic (RP-HPLC) method has been developed for the determination of gemifloxacin in bulk, dosage formulations and human serum at 270 nm. Chromatographic separation was achieved on Purospher STAR C18 (250 × 4.6 mm, 5 µm) column using mobile phase methanol:water (90:10, v/v) adjusted pH 2.8 via phosphoric acid 85% having flow rate of 1.5 mL min-1 at ambient temperature. Calibration curves were linear over range of 5-100 µg mL-1 with a correlation coefficient 0.9998. The limit of detection (LOD) and limit of quantitation (LOQ) were 0.015 and 0.045 µg mL-1, respectively. Intra and inter-run precision and accuracy results were 98.73-100.12% and then correlated through student'st-test. This method was further applied for in vitro interactions of gemifloxacin with essential and trace elements.

Gemifloxacin

Phosphoric acid

Linear correlation

Linear range

10.1590/s0103-50532011000500024

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Citations (14)

SIMULTANEOUS DETERMINATION OF CEFTRIAXONE SODIUM AND STATIN DRUGS IN PHARMACEUTICAL FORMULATIONS AND HUMAN SERUM BY RP-HPLC

Journal of the Chilean Chemical Society (2010)

Najma Sultana M. Saeed Arayne Waseem Shahzad

An accurate, sensitive and least time consuming reverse phase high performance liquid chromatographic (RP-HPLC) method for the estimation of ceftriaxone in the presence of statin drugs in formulation and human serum has been developed and validated.Chromatographic separation was conducted on prepacked Purospher Star, C18 (5 mm, 250 x 4.6 mm) column at room temperature using methanol:water:acetonitrile (70:15:20 v/v/v) as a mobile phase, pH adjusted at 2.8 with ortho-phosphoric acid and at a flow rate of 1.0 mL/minute, while UV detection was performed at 240 nm.The results obtained showed a good agreement with the declared content.The method shows good linearity in the range of 2.5-25 µg/mL with a correlation coefficient 0.9966 -0.9998 (inter-and intra-day RSD < 2 %).The limit of detection and quantification for ceftriaxone and statins in pharmaceutical formulation and serum were in the range 0.124 -1.006 µg/mL.Analytical recovery was >98.72 %.The proposed method may be used for the quantitative analysis of commonly administered statin's i.e. simvastatin, rosuvastatin atorvastatin and pravastatin alone or in combination with ceftriaxone from raw materials, dosage formulations and in serum.The established HPLC method is rapid, accurate and selective, because of its sensitivity and reproducibility.

10.4067/s0717-97072010000200010

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Citations (21)