Humberto Debat

National Agricultural Technology Institute

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Nicolás Bejerman

National Agricultural Technology Institute

Mauro Grabiele

National University of Misiones

Daniel A. Ducasse

National Agricultural Technology Institute

Patricia M. Aguilera

National University of Misiones

Sebastián Asurmendi

Consejo Nacional de Investigaciones Científicas y Técnicas

Ralf G. Dietzgen

University of Queensland

Diego Zavallo

National Agricultural Technology Institute

Sebastián Gómez‐Talquenca

National Agricultural Technology Institute

Benjamin Schwessinger

Australian National University

Carolina Torres

University of Buenos Aires

Cooperative Institutions

Consejo Nacional de Investigaciones Científicas y Técnicas

153

National Agricultural Technology Institute

University of Buenos Aires

Hospital General de Niños Ricardo Gutierrez

Ministerio de Salud

Charité - Universitätsmedizin Berlin

Centro Científico Tecnológico - San Juan

Instituto de Fisiología Vegetal

Instituto de Biotecnología y Biología Molecular

Universidad Nacional de Córdoba

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A barnavirus sequence mined from a transcriptome of the Antarctic pearlwort Colobanthus quitensis

Archives of Virology (2018)

Max L. Nibert Austin R. Manny Humberto Debat Andrew E. Firth Laura Bertini

Because so few viruses in the family Barnaviridae have been reported, we searched for more of them in public sequence databases. Here, we report the complete coding sequence of Colobanthus quitensis associated barnavirus 1, mined from a transcriptome of the Antarctic pearlwort Colobanthus quitensis. The 4.2-kb plus-strand sequence of this virus encompasses four main open reading frames (ORFs), as expected for barnaviruses, including ORFs for a protease-containing polyprotein, an RNA-dependent RNA polymerase whose translation appears to rely on − 1 ribosomal frameshifting, and a capsid protein that is likely to be translated from a subgenomic RNA. The possible derivation of this virus from a fungus associated with C. quitensis is discussed.

ORFS

Subgenomic mRNA

10.1007/s00705-018-3794-x

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Citations (17)

First report of grapevine Roditis leaf discoloration-associated virus infecting grapevine in Argentina

Journal of Plant Pathology (2025)

Roberto Cuello Humberto Debat Diego Zavallo Marianna Volpe Javier Matías Prieto

10.1007/s42161-025-01851-z

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A Survey of Mycoviral Infection in Fusarium spp. Isolated from Maize and Sorghum in Argentina Identifies the First Mycovirus from Fusarium verticillioides

Viruses (2020)

Andrés Gustavo Jacquat Martín G. Theumer M. Carmen Cañizares Humberto Debat Juliana Iglesias

Mycoviruses appear to be widespread in Fusarium species worldwide. The aim of this work was to identify mycoviral infections in Fusarium spp., isolated from maize and sorghum grown in Argentina, and to estimate their potential effects on the pathogenicity and toxigenesis of the host fungus towards maize. Mycoviruses were identified in 2 out of 105 isolates analyzed; Fusarium verticillioides strain Sec505 and Fusarium andiyazi strain 162. They were characterized as members of the genus Mitovirus by high-throughput sequencing and sequence analysis. The F. verticillioides mitovirus was a novel mycovirus whereas the F. andiyazi mitovirus was found to be a new strain of a previously identified mitovirus. We have named these mitoviruses, Fusarium verticillioides mitovirus 1 (FvMV1) and Fusarium andiyazi mitovirus 1 strain 162 (FaMV1-162). To our knowledge, FvMV1 is the first mycovirus reported as naturally infecting F. verticillioides, the major causal agent of ear rot and fumonisin producer in corn. Both mitoviruses exhibited 100% vertical transmission rate to microconidia. The Fa162 strain infected with FaMV1-162 did not show phenotypic alterations. In contract, F. verticillioides Sec505 infected with FvMV1 showed increased virulence as well as microconidia and fumonisin-B1 production, compared with two uninfected strains. These results suggest that FvMV1 could have a role in modulating F. verticillioides pathogenicity and toxin production worth further exploring.

Mycovirus

Fumonisin

Strain (injury)

10.3390/v12101161

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Citations (16)

Firefly genomes illuminate parallel origins of bioluminescence in beetles

bioRxiv (Cold Spring Harbor Laboratory) (2017)

Timothy Fallon Sarah E. Lower Ching-Ho Chang Manabu Bessho‐Uehara Gavin J. Martin

Abstract Fireflies and their fascinating luminous courtships have inspired centuries of scientific study. Today firefly luciferase is widely used in biotechnology, but the evolutionary origin of their bioluminescence remains unclear. To shed light on this long-standing question, we sequenced the genomes of two firefly species that diverged over 100 million-years-ago: the North American Photinus pyralis and Japanese Aquatica lateralis. We also sequenced the genome of a related click-beetle, the Caribbean Ignelater luminosus, with bioluminescent biochemistry near-identical to fireflies, but anatomically unique light organs, suggesting the intriguing but contentious hypothesis of parallel gains of bioluminescence. Our analyses support two independent gains of bioluminescence between fireflies and click-beetles, and provide new insights into the genes, chemical defenses, and symbionts that evolved alongside their luminous lifestyle. One Sentence Summary: Comparative analyses of the first linkage-group-resolution genomes of fireflies and related bioluminescent beetles address long-standing questions of the origin and evolution of bioluminescence and its associated traits.

Lampyridae

Luciferases

Luciferin

10.1101/237586

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Citations (7)

Identification of novel RNA viruses associated to bird’s-foot trefoil (Lotus corniculatus)

bioRxiv (Cold Spring Harbor Laboratory) (2018)

Humberto Debat Nicolás Bejerman

Bird9s-foot trefoil ( Lotus corniculatus L.) is a highly nutritious forage crop, employed for livestock foraging around the world. Despite the agronomical importance of this resilient crop, the related literature is rather scarce. Here, we report the identification and characterization of two novel viruses associated with bird9s-foot trefoil. Virus sequences with affinity to enamoviruses (ssRNA (+); Luteoviridae ; Enamovirus ) and nucleorhabdoviruses (ssRNA (-); Rhabdoviridae ; Nucleorhabdovirus ) were detected in L. corniculatus transcriptome data. The proposed bird9s-foot trefoil enamovirus 1 (BFTEV-1) 5,736 nt virus sequence presents a typical 59-PO-P1-2-IGS-P3-P5-39; enamovirus genome structure. The tentatively named bird9s-foot trefoil nucleorhabdovirus (BFTNRV) genome organization is characterized by 13,626 nt long negative-sense single-stranded RNA. BFTNRV presents in its antigenome orientation six predicted gene products in the canonical order 39-N-P-P3-M-G-L-59. Phylogenetic analysis suggests that BFTNRV is closely related to Datura yellow vein nucleorhabdovirus , and that BFTEV-1 clusters into a monophyletic clade of legumes-associated enamoviruses. The bioinformatic reanalysis of SRA libraries deposited in the NCBI database constitutes an emerging approach to the discovery of novel plant viruses. The RNA viruses reported here provide a first glimpse of the virus landscape of this important crop. Future studies should assess the prevalence of BFTEV-1 and BFTNRV, and unravel whether the infection of these novel viruses is associated to specific symptoms.

Lotus corniculatus

Trefoil

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Correction to: 2021 Taxonomic update of phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales

Archives of Virology (2021)

Jens H. Kuhn Scott Adkins Bernard Agwanda Rim Al Kubrusli Sergey V. Alkhovsky

10.1007/s00705-021-05266-w

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Molecular Identification and Characterization of Two Rubber Dandelion Amalgaviruses

bioRxiv (Cold Spring Harbor Laboratory) (2017)

Humberto Debat Zinan Luo Brian J. Iaffaldano Xiaofeng Zhuang Katrina Cornish

Abstract The Amalgaviridae family comprise persistent viruses that share the genome architecture of Totiviridae and gene evolutionary resemblance to Partitiviridae . Two genera have been assigned to this family, including genus Amalgavirus consisting in nine recognized species, corresponding to plant infecting viruses with dsRNA monosegmented genomes of ca. 3.4 kb. Here, we present the molecular characterization of two novel viruses detected in rubber dandelion ( Taraxacum kok-saghyz ). The sequenced viruses are 3,409 and 3,413 nt long, including two partially overlapping ORFs encoding a putative coat protein and an RNA-dependent RNA polymerase (RdRP). Phylogenetic insights based on the RdRP suggest them to be members of two new species within the Amalgavirus genus. Multiple independent RNAseq data suggest that the identified viruses have a dynamic distribution and low relative RNA levels in infected plants. Virus presence was not associated with any apparent symptoms on the plant hosts. We propose the names rubber dandelion latent virus 1 & 2 to the detected amalgaviruses; the first viruses to be associated to this emergent and sustainable natural rubber crop.

ORFS

RNA Silencing

Human virome

10.1101/229443

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Complete genome sequence of a divergent strain of Tibetan frog hepatitis B virus associated to concave-eared torrent frogOdorrana tormota

bioRxiv (Cold Spring Harbor Laboratory) (2018)

Humberto Debat Terry Fei Fan Ng

Abstract The family Hepadnaviridae is characterized by partially dsDNA circular viruses of approximately 3.2 kb, which are reverse transcribed from RNA intermediates. Hepadnaviruses (HBVs) have a broad host range which includes humans (Hepatitis B virus), other mammals (genus Orthohepadnavirus ), and birds ( Avihepadnavirus ). HBVs host specificity has been expanded by reports of new viruses infecting fish, amphibians, and reptiles. The tibetan frog hepatitis B virus (TFHBV) was recently discovered in Nanorana parkeri (Family Dicroglossidae ) from Tibet. To increase understanding of hepadnavirus in amphibian host, we identified the full-length genome of a divergent strain TFHBV-Ot associated to the concave-eared torrent frog Odorrana tormota (Family Ranidae ) from China by searching deep sequencing data. TFHBV-Ot shared the genomic organization and a 76.6% overall genome nucleotide identity to the prototype TFHBV associated to N. parkeri (TFHBV-Np). TFHBV-Ot amino acid pairwise identity with TFHBV-Np predicted gene products ranged between 63.9% and 77.9%. Multiple tissue/organ specific RNAseq datasets suggest a broad tropism of TFHBV including muscles, gonads and brains. In addition, we provide for the first time evidence of virus derived small RNA from an amphibian hepadnavirus, tentatively enriched in 19-20 nt species and cytidine as first base. The results presented here expand the genetic diversity and the host range of TFHBV to Ranidae frogs, and warrant investigation on hepadnaviral infection of amphibian brains.

10.1101/506550

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Expanding the Repertoire of the Plant-Infecting Ophioviruses through Metatranscriptomics Data

Viruses (2023)

Humberto Debat María Laura García Nicolás Bejerman

Ophioviruses (genus Ophiovirus, family Aspiviridae) are plant-infecting viruses with non-enveloped, filamentous, naked nucleocapsid virions. Members of the genus Ophiovirus have a segmented single-stranded negative-sense RNA genome (ca. 11.3-12.5 kb), encompassing three or four linear segments. In total, these segments encode four to seven proteins in the sense and antisense orientation, both in the viral and complementary strands. The genus Ophiovirus includes seven species with viruses infecting both monocots and dicots, mostly trees, shrubs and some ornamentals. From a genomic perspective, as of today, there are complete genomes available for only four species. Here, by exploring large publicly available metatranscriptomics datasets, we report the identification and molecular characterization of 33 novel viruses with genetic and evolutionary cues of ophioviruses. Genetic distance and evolutionary insights suggest that all the detected viruses could correspond to members of novel species, which expand the current diversity of ophioviruses ca. 4.5-fold. The detected viruses increase the tentative host range of ophioviruses for the first time to mosses, liverwort and ferns. In addition, the viruses were linked to several Asteraceae, Orchidaceae and Poaceae crops/ornamental plants. Phylogenetic analyses showed a novel clade of mosses, liverworts and fern ophioviruses, characterized by long branches, suggesting that there is still plenty of unsampled hidden diversity within the genus. This study represents a significant expansion of the genomics of ophioviruses, opening the door to future works on the molecular and evolutionary peculiarity of this virus genus.

Orchidaceae

10.3390/v15040840

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Citations (9)

A glimpse into the DNA virome of the unique “living fossil” Welwitschia mirabilis

Gene (2022)

Humberto Debat Nicolás Bejerman

Human virome

Lineage (genetic)

genomic DNA

Geminiviridae

10.1016/j.gene.2022.146806

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Citations (6)