Xin Guan

Peking University Shenzhen Hospital

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Yongqing Lai

Peking University Shenzhen Hospital

Jinling Xu

General Hospital of Central Theater Command

Liwen Zhao

Peking University Shenzhen Hospital

Jing Quan

Peking University Shenzhen Hospital

Xiang Pan

Nanjing University

Peijie Chen

Tianjin Medical University General Hospital

Zuwei Li

Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine

Liangchao Ni

Peking University Shenzhen Hospital

Weijie Xu

Beijing Forestry University

Shangqi Yang

Peking University Shenzhen Hospital

Cooperative Institutions

Peking University Shenzhen Hospital

Peking University

Northeast Agricultural University

Chinese Academy of Sciences

Harbin Medical University

Chinese Academy of Medical Sciences & Peking Union Medical College

University of Hong Kong - Shenzhen Hospital

Shanghai Jiao Tong University

University of Chinese Academy of Sciences

Anhui Medical University

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miR-566 functions as an oncogene and a potential biomarker for prognosis in renal cell carcinoma

Biomedicine & Pharmacotherapy (2018)

Xiang Pan Jing Quan Zuwei Li Liwen Zhao Liang Zhou

Renal cell carcinoma (RCC), a heterogeneous type of cancer originating from the nephron, occupies approximately 3.9% of new carcinomas, with an increasing incidence in the past two decades. The most common subtype of renal cell carcinoma is clear cell RCC (ccRCC). Though surgery and other treatments are applied to RCC, it has the highest recurrence rate and mortality rate among the genitourinary cancers. As the study progressed, miRNAs are found to be the biomarkers for tumor diagnosis, prognosis and the targets for tumor management.In present study, RT-qPCR, wound scratch assay, cell proliferation assay, transwell assay and flow cytometry assay were performed to ascertain miR-566 expression level and its proliferation, migration and apoptosis in RCC. Moreover, we analyzed the relation between miR-566 expression and clinicopathological variables or overall survival from the 42 formalin-fixed paraffin-embedded (FFPE) renal cancer samples. We further evaluate prognostic values of miR-566 expression.miR-566 is up-regulated in RCC tissue samples and renal carcinoma cell lines. miR-566 promotes cell proliferation, mobility and inhibits cell apoptosis in 786-O and ACHN cell lines. Cox proportional hazard regression analysis indicates that low expression of miR-566 patients have a remarkable longer overall survival in the univariate and multivariate analysis. The Kaplan-Meier survival curves show that the low expression of miR-566 patients have a remarkable longer overall survival.The results of the current study demonstrate that oncogene miR-566 is a potential biomarker not only for diagnosis but also for prognosis for RCC.

Kidney cancer

10.1016/j.biopha.2018.03.072

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A genome-wide survey of homeodomain-leucine zipper genes and analysis of cold-responsive HD-Zip I members’ expression in tomato

Bioscience Biotechnology and Biochemistry (2014)

Zhenzhu Zhang Xiuling Chen Xin Guan Yang Liu Hongyu Chen

Homeodomain-leucine zipper (HD-Zip) proteins are a kind of transcriptional factors that play a vital role in plant growth and development. However, no detailed information of HD-Zip family in tomato has been reported till now. In this study, 51 HD-Zip genes (SlHZ01-51) in this family were identified and categorized into 4 classes by exon-intron and protein structure in tomato (Solanum lycopersicum) genome. The synthetical phylogenetic tree of tomato, Arabidopsis and rice HD-Zip genes were established for an insight into their evolutionary relationships and putative functions. The results showed that the contribution of segmental duplication was larger than that of tandem duplication for expansion and evolution of genes in this family of tomato. The expression profile results under abiotic stress suggested that all SlHZ I genes were responsive to cold stress. This study will provide a clue for the further investigation of functional identification and the role of tomato HD-Zip I subfamily in plant cold stress responses and developmental events.

Subfamily

Segmental duplication

10.1080/09168451.2014.923292

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Citations (48)

Actin marker lines in grapevine reveal a gatekeeper function of guard cells

Journal of Plant Physiology (2014)

Xin Guan Günther Buchholz Peter Nick

Plasmopara viticola

10.1016/j.jplph.2014.03.019

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Citations (14)

Supplementary videos for "Interplay Between Convective and Viscoelastic Forces Controls the Morphology of in vitro Paclitaxel- Stabilized Microtubules"

Zenodo (CERN European Organization for Nuclear Research) (2019)

Eftihia Barnes Xin Guan Erik M. Alberts Travis L. Thornell Christopher M. Warner

Supplementary videos for MDPI Crystals manuscript "Interplay Between Convective and Viscoelastic Forces Controls the Morphology of in vitro Paclitaxel- Stabilized Microtubules "

Morphology

10.5281/zenodo.3517065

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microRNA‑181a‑5p functions as an oncogene in renal cell carcinoma

Molecular Medicine Reports (2018)

Yulin Lai Liwen Zhao Jia Hu Jing Quan Peijie Chen

Renal cell carcinoma (RCC) is one of the most common urinary tumors. Previous studies have demonstrated that microRNA (miR)‑181a‑5p has an important role in numerous types of cancer. However, the function of miR‑181a‑5p in RCC remains unknown. In the present study, the expression levels of miR‑181a‑5p in RCC tissues and cell lines were investigated using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) analysis. The results of the RT‑qPCR analyses suggested that the expression of miR‑181a‑5p was upregulated in RCC tissues and cells lines compared with adjacent normal renal tissues and normal renal cell lines. Furthermore, the effect of miR‑181a‑5p on cell proliferation, migration, invasion and apoptosis was investigated in the present study. Overexpression of miR‑181a‑5p was revealed to suppress the apoptosis of 786‑O and ACHN cells, in addition to enhancing the proliferation, migration and invasion abilities of 786‑O and ACHN cells in vitro, thus suggesting that miR‑181a‑5p may function as an oncogene in RCC. However, further studies are required to investigate the underlying mechanism of miR‑181a‑5p and its potential role as a biomarker for early detection and prognosis, in addition to as a therapeutic target in RCC.

10.3892/mmr.2018.8899

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Comprehensive bioinformatics analysis reveals the prognostic value, predictive value, and immunological roles of ANLN in human cancers

Frontiers in Genetics (2022)

Zhiwei Cui Jiantao Mo Ping Song Lijun Wang Rongli Wang

Anillin (ANLN) is a unique scaffolding, actin-binding protein, which is essential for the integrity and ingression of the cleavage furrow. It is mainly involved in the cytokinesis process, while its role in various tumors has not been fully addressed and remains largely elusive. To provide a thorough perspective of ANLN's roles among diverse malignancies, we conducted a comprehensive, pan-cancer analysis about ANLN, including but not limited to gene expression levels, prognostic value, biological functions, interacting proteins, immune-related analysis, and predictive value. As a result, when compared to normal tissues, ANLN expression is elevated in most cancers, and its expression also differs in different immune subtypes and molecular subtypes in diverse cancers. In addition, in 17 types of cancer, ANLN expression is increased in early tumor stages, and higher ANLN expression predicts worse survival outcomes in more than ten cancers. Furthermore, ANLN shows close correlations with the infiltration levels of most immune cells, and enrichment analysis using ANLN co-expressed genes reveals that ANLN plays essential roles in cell cycle, mitosis, cellular senescence, and p53 signaling pathways. In the final, ANLN exhibits high accuracy in predicting many cancers, and subsequent multivariate analysis suggests ANLN could be an independent prognostic factor in specific cancer types. Taken together, ANLN is proved to be a novel and promising biomarker for its excellent predictive utility, promising prognostic value, and potential immunological roles in pan-cancer. Targeting ANLN might be an attractive approach to tumor treatment.

10.3389/fgene.2022.1000339

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microRNA‑572 functions as an oncogene and a potential biomarker for renal cell carcinoma prognosis

Oncology Reports (2018)

Xiang Pan Zuwei Li Liwen Zhao Jing Quan Liang Zhou

Renal cell carcinoma (RCC) is the third most common urological malignancy in the USA and represents 2‑3% of all adult malignancies. Furthermore, the incidence of RCC has been progressively increasing over recent years. Although the morbidity of treatment has decreased with the use of multidisciplinary synthetic therapy, the prognosis of terminal cancer remains poor, with a 5‑year survival rate of 5‑10%. MicroRNAs (miRs) have been correlated with the regulation of 30‑60% of the protein-coding genes and act as oncogenes or anti‑oncogenes in RCC. Considering this research, miRNAs are likely to be the biomarkers for tumor diagnosis, prognosis and the targets for RCC management. In the present study, 42 formalin‑fixed paraffin‑embedded RCC samples were used. The expression of miR‑572 and the role of miR‑572 in RCC cell proliferation, migration and apoptosis was determined by performing reverse transcription‑quantitative polymerase chain reaction analysis, wound scratch assays, cell proliferation assays, Transwell assays and flow cytometry assays, respectively. Further experiments were conducted to clarify the correlation between miR‑572 expression and clinicopathological variables or overall survival. Furthermore, the expression levels of miR‑572 were evaluated for the prognosis value of patients with RCC. Upregulation of miR‑572 was observed in RCC tissues and RCC cell lines. miR‑572 promoted 786‑O and ACHN cell proliferation and mobility and inhibited early apoptosis. In Cox proportional hazard regression analyses, results of the univariate and multivariate analysis indicated that the patients with low miR‑572 expression had a significantly longer overall survival compared with the patients with high miR‑572 expression (univariate analysis, P=0.037; multivariate analysis, P=0.034). Results of the Kaplan‑Meier survival curves revealed that the patients with downregulated miR‑572 have a significantly longer overall survival compared with the patients with highly expressed miR‑572 (P=0.019). To conclude, the results of the present study suggest that tumor oncogene miR‑572 is a potential biomarker for the diagnosis, treatment and prognosis for RCC.

10.3892/or.2018.6649

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Citations (13)

Genome-Wide Identification and Characterisation of Stress-Associated Protein Gene Family to Biotic and Abiotic Stresses of Grapevine

Pathogens (2022)

Xiaoye Sun Xue Xia Xin Guan

Grapevine is one of the earliest domesticated fruit crops and prized for its table fruits and wine worldwide. However, the concurrence of a number of biotic/abiotic stresses affects their yield. Stress-associated proteins (SAPs) play important roles in response to both biotic and abiotic stresses in plants. Despite the growing number of studies on the genomic organisation of SAP gene family in various species, little is known about this family in grapevines (Vitis vinifera L.). In this study, a total of 15 genes encoding proteins possessing A20/AN1 zinc-finger were identified based on the analysis of several genomic and proteomic grapevine databases. According to their structural and phylogenetics features, the identified SAPs were classified into three main groups. Results from sequence alignments, phylogenetics, genomics structure and conserved domains indicated that grapevine SAPs are highly and structurally conserved. In order to shed light on their regulatory roles in growth and development, as well as the responses to biotic/abiotic stresses in grapevine, the expression profiles of SAPs were examined in publicly available microarray data. Bioinformatics analysis revealed distinct temporal and spatial expression patterns of SAPs in various tissues, organs and developmental stages, as well as in response to biotic/abiotic stresses. This study provides insight into the evolution of SAP genes in grapevine and may aid in efforts for further functional identification of A20/AN1-type proteins in the signalling cross-talking induced by biotic/abiotic stresses.

Biotic stress

Biotic component

Identification

10.3390/pathogens11121426

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Citations (4)

[Corrigendum] miR‑199b‑5p serves as a tumor suppressor in renal cell carcinoma

Experimental and Therapeutic Medicine (2024)

Yulin Lai Jing Quan Canbin Lin Hang Li Jia Hu

Following the publication of the above article and a corrigendum (10.3892/etm.2018.6333) that was concerned with changes in the authorship and affiliation details on the paper, an interested reader has drawn to the authors’ attention that, in Fig. 5A on p. 441, the ‘ACHN/cell invasion/miR‑199b‑5p inhibitor’ and ‘ACHN/cell invasion/NCin’ data panels appeared to show an overlapping section of data, such that they were derived from the same original source when they were intended to show the results from differently performed experiments.
The authors have re‑examined their data, and realize that the ‘ACHN/cell invasion/NCin’ data panel was inadvertently selected incorrectly. The corrected version of Fig. 5, now containing the correct data for the ‘ACHN/cell invasion/NCin’ experiment, is shown on the next page. Note that the error made during the compilation of this figure did not affect the overall conclusions reported in the paper. All the authors agree with the publication of this corrigendum, and are grateful to the Editor of Experimental and Therapeutic Medicine for offering them the opportunity to publish this. They also apologize to the readership for any inconvenience caused. [Experimental and Therapeutic Medicine 16: 436‑444, 2018; DOI: 10.3892/etm.2018.6151]

10.3892/etm.2024.12422

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Studies on gene transfer of shoot apical meristems by Agrobacterium-mediated genetic transformation in a progeny of Chinese wild Vitis pseudoreticulata

Vitis (2013)

Xin Guan Heqing Zhao Yan Xu Yuejin Wang

Shoot tips and/or single-bud-internodes, containing shoot apical meristems (SAM) were presented, of Vitis '6-12-2', derived from a Chinese wild V. pseudoreticulata 'Baihe-35-1' × V. vinifera 'Carignane' cross, were used for Agrobacterium -mediated genetic transformation. In order to achieve Glyoxal oxidase (VpGLOX) overexpressing plants, the propagation and gene transfer system of shoot tips and/or single-bud-internodes undergoing either micropropagated (starting with microshoot- tips) or callus induced (starting with stems with single bud) in vitro plants were optimized. The results show that the most effective way to gain shoot tips and/or single-bud-internodes undergoing micropropagation procedure was to keep micro-shoot-tips in liquid C 2 D4B medium at 80 rpm constant orbital shaking with light, then placed on solidified C 2 D4B medium with 2.9 μM Gibberellic acid 3 (GA3) for elongation. In vitro stems with single buds gave best results for callus formation and adventitious buds induction on half-strength MS medium with 9.0 μM Thidiazuron (TDZ) and 2.9 μM mg·L -1 α-Naphthaleneacetic acid (NAA). The highest gene transfer frequency was obtained when explants were infected for 10 min with the concentration of Agrobacterium tumefaciens with an optical density at 600 nm of 0.4, and then co-cultivated for 3 days. Incubation of shoot tips and/or single-bud-internodes in darkness for 3 days is helpful for enhancing gene transfer efficiency. Polymerase chain reaction (PCR) and PCR-Southern blot analyses were utilized to confirm putative transgenic plants. Up to 45 clones have proven to be transformed, and one of them has been planted out. This method opens a door for the gene transfer of recalcitrant Chinese wild V. pseudoreticulata .

Plant stem

Callus

Gibberellic acid

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Citations (6)