Carla Lima

Instituto Butantan

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Mônica Lopes‐Ferreira

Instituto Butantan

Geonildo Rodrigo Disner

Instituto Butantan

Maria Alice Pimentel Falcão

Universidade Federal de Sergipe

Lidiane Zito Grund

Instituto Butantan

Kátia Conceição

Universidade Federal de São Paulo

Evilin Naname Komegae

Fundação de Amparo à Pesquisa do Estado de São Paulo

Adolfo Luís Almeida Maleski

Instituto Butantan

Fernanda Miriane Bruni

Instituto Butantan

Vidal Haddad

Universidade Estadual Paulista (Unesp)

João Gabriel Santos Rosa

Fundação de Amparo à Pesquisa do Estado de São Paulo

Cooperative Institutions

John Wiley & Sons (United States)

327

Ecological Society of America

326

IFC Research (United Kingdom)

310

Universidade de São Paulo

127

Instituto Butantan

124

Wyoming Game and Fish Department

110

Universidade Estadual Paulista (Unesp)

Fundação de Amparo à Pesquisa do Estado de São Paulo

Institute of Biomedical Science

Universidade Federal de São Paulo

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Acute-phase proteins during inflammatory reaction by bacterial infection: Fish-model

Scientific Reports (2019)

Ives Charlie‐Silva André Klein Juliana Moreira Mendonça-Gomes Ed Johnny da Rosa Prado Alessandra Cristina de Moraes

Acute-phase protein (APPs) serum levels have been studied in many human diseases, and their components contribute to host defense during the evolution of infectious diseases by acting as part of the innate immune system. Based on the importance of establishing new experimental models, the present investigation evaluated the modulation of APPs following inflammatory stimulus by the inoculation of Aeromonas hydrophila in tilapias. Fish were sampled 6 and 24 hours post-infection. Tilapias presented increase of positive APPs such as ceruloplasmin, haptoglobin, alpha-2-macroglobulin and complement C3, as well as decrease of negative APPs such as albumin and transferrin. The protein response of tilapias during the course of bacterial infection showed correlation with the kinetics of cellular accumulation in the inflamed focus with significant increase of granulocytes, thrombocytes, lymphocytes and macrophages. However, granulocytes were the predominant cells, associated with increment in the reactive oxygen species (ROS) production. Showing responses similar to those observed in humans, the modulation of APPs and the kinetics of cellular accumulation in the exudate demonstrate the feasibility of this alternative experimental model for advances and studies to understand changes in pathophysiological mechanisms of acute inflammatory reaction due to bacterial infection.

10.1038/s41598-019-41312-z

Cite

Citations (80)

Studies on pharmacological properties of mucus and sting venom of Potamotrygon cf. henlei

International Immunopharmacology (2011)

Juliane Monteiro-dos-Santos Kátia Conceição Carla Simone Seibert Elineide Eugênio Marques Pedro Ismael da Silva

Stingrays from the Potamotrygon cf. henlei species are widely distributed in high numbers throughout the rivers of central-west Brazil, being the source of numerous envenomations occurring in the dry season, posing a serious public health problem even if not properly reported. The accidents usually involve fishermen and bathers, and to date there is no effective treatment for the injured. Considering these facts and limitations of studies aiming at understanding the effects induced by P. cf. henlei envenoming, this study aimed to describe the principal pharmacological and certain biochemical properties of the mucus and sting venom. We found that mucus and sting venom is toxic to mice having nociceptive, edematogenic and proteolysis activities. Our results also indicate that the inflammatory cellular influx observed could be triggered by the venom and mucus. Furthermore the venom and mucus were partially purified by solid-phase extraction tested for antimicrobial activity in which only the mucus presented activity. It could be inferred from the present study that P. cf. henlei venom possesses a diverse mixture of peptides, enzymes and pharmacologically active components.

Sting

Proteolysis

10.1016/j.intimp.2011.03.019

Cite

Citations (41)

Venomous Freshwater Fish: Catfish and Freshwater Stingrays

Springer eBooks (2016)

Vidal Haddad Carla Lima Mônica Lopes‐Ferreira

Fresh water fish

10.1007/978-94-007-6419-4_11

Cite

Citations (1)

Interference of methysergide, a specific 5‐hydroxytryptamine receptor antagonist, with airway chronic allergic inflammation and remodelling in a murine model of asthma

Clinical & Experimental Allergy (2007)

Carla Lima Valdênia Maria Oliveira de Souza Alexandre Learth Soares Mahasti Sahihi de Macedo Wothan Tavares-de-Lima

Summary Background Airway remodelling encompasses the structural changes observed in asthmatic airways. Mast cells, through the release of histamine and 5‐hydroxytryptamine (serotonin), are implicated in early asthmatic reactions, bronchoconstriction and mucosal oedema, and in the development of bronchial hyperresponsiveness. However, the association between serotonin and remodelling processes in murine model of airways inflammation remains to be elucidated. Objective As serotonin is released by murine mast cells upon antigen challenge, we tested the hypothesis of its involvement in the development of inflammatory and remodelling processes in a murine model of chronic airway inflammation following prolonged allergen challenge. Methods BALB/c mice were exposed to aerosolized ovalbumin for 20 min 2 days a week, for 4 consecutive weeks. Two hours before each challenge, they were treated with methysergide (intranasally, 40 μg/kg). Forty‐eight hours after the last aerosol challenge, bronchoalveolar lavage (BAL) and lung tissue were collected for analysis. Results Methysergide inhibited the allergen‐induced increase in airway eosinophilia, reduced T helper type 2 (Th2) cytokines in lung, spleen or thoracic lymph nodes, and specific IgE levels. The extravasation of plasma and fibronectin production in the lung, and collagen deposition in the lung were also inhibited after methysergide treatment. Although methysergide treatment induced an increase in IFN‐γ levels, experiments with neutralizing antibody suggest that this is not responsible for inhibition. In addition, instillation of serotonin to immunized mice induced eosinophil recruitment to BAL, Th2 cytokine production and fibronectin release in lung as well as collagen deposition. Conclusion Serotonin may contribute to the development and maintenance of remodelling through the release of cytokines and of fibrogenic mediators. Serotonin should therefore be considered as relevant for the development and maintenance of airway remodelling.

Methysergide

10.1111/j.1365-2222.2007.02700.x

Cite

Citations (30)

JUSTIÇA CLIMÁTICA E O PROTAGONISMO DAS MULHERES EXTRATIVISTAS: UM ESTUDO BIBLIOMÉTRICO

Editora Científica Digital eBooks (2024)

Carla Lima Ires das Dores Borges Costa Maria de Nazaré Lopes de Macedo Suzana Mendonça de Sales Luiz Cláudio Moreira Melo Júnior

A interseção entre racismo ambiental e protagonismo feminino é um tema relevante e emergente, especialmente no contexto das mudanças climáticas e da justiça ambiental. Esta pesquisa objetivou realizar um estudo sobre o papel das mulheres extrativistas na preservação dos ecossistemas e na promoção de práticas sustentáveis, destacando a importância de reconhecer e valorizar suas contribuições. A metodologia envolveu pesquisa qualitativa e medição bibliométrica, com análise de artigos científicos, teses e dissertações nas bases de dados SciELO, Google Acadêmico e Periódicos CAPES. Foram selecionados 23 artigos que abordavam as temáticas de justiça climática, justiça ambiental e protagonismo feminino na Amazônia. Os resultados revelaram um aumento significativo de produções, a partir de 2020, ressaltando a importância de incluir a presença feminina nos diálogos sobre questões ambientais e de justiça climática. A análise apontou para a necessidade de ampliar o reconhecimento e a valorização do papel das mulheres extrativistas como líderes e defensoras do meio ambiente, contribuindo para economias locais, segurança alimentar e resiliência diante das ameaças climáticas e ambientais. No entanto, também evidenciou a escassez de literatura que aborde o protagonismo das mulheres, destacando a persistência de processos de dominação, exploração e violência, que historicamente silenciaram e continuam a dificultar o acesso das mulheres a seus direitos sociais de acesso às políticas públicas

10.37885/241017913

Cite

Citations (0)

TLR2, TLR4 and the MyD88 Signaling Are Crucial For the In Vivo Generation and the Longevity of Long-Lived Antibody-Secreting Cells

PLoS ONE (2013)

Evilin Naname Komegae Lidiane Zito Grund Mônica Lopes‐Ferreira Carla Lima

This study was undertaken to gain better insights into the role of TLRs and MyD88 in the development and differentiation of memory B cells, especially of ASC, during the Th2 polarized memory response induced by Natterins. Our in vivo findings demonstrated that the anaphylactic IgG1 production is dependent on TLR2 and MyD88 signaling, and that TLR4 acts as adjuvant accelerating the synthesis of high affinity-IgE. Also, TLR4 (MyD88-independent) modulated the migration of innate-like B cells (B1a and B2) out of the peritoneal cavity, and the emigration from the spleen of B1b and B2 cells. TLR4 (MyD88-independent) modulated the emigration from the spleen of Bmem as well as ASC B220(pos). TLR2 triggered to the egress from the peritoneum of Bmem (MyD88-dependent) and ASC B220(pos) (MyD88-independent). We showed that TLR4 regulates the degree of expansion of Bmem in the peritoneum (MyD88-dependent) and in BM (MyD88-independent) as well as of ASC B220(neg) in the spleen (MyD88-independent). TLR2 regulated the intensity of the expansion of Bmem (MyD88-independent) and ASC B220(pos) (MyD88-dependent) in BM. Finally, TLR4 signals sustained the longevity of ASC B220(pos) (MyD88-independent) and ASC B220(neg) into the peritoneum (MyD88-dependent) and TLR2 MyD88-dependent signaling supported the persistence of B2 cells in BM, Bmem in the spleen and ASC B220(neg) in peritoneum and BM. Terminally differentiated ASC B220(neg) required the cooperation of both signals through TLR2/TLR4 via MyD88 for longevity in peritoneum, whereas Bmem required only TLR2/MyD88 to stay in spleen, and ASC B220(pos) rested in peritoneum dependent on TLR4 signaling. Our data sustain that earlier events on memory B cells differentiation induced in secondary immune response against Natterins, after secondary lymph organs influx and egress, may be the key to determining peripheral localization of innate-like B cells and memory B cells as ASC B220(pos) and ASC B220(neg).

10.1371/journal.pone.0071185

Cite

Citations (24)

Venomous Marine Fish: Osteichthyes (Bony Fish)

Springer eBooks (2016)

Vidal Haddad Carla Lima Mônica Lopes‐Ferreira

Toadfish

Marine fish

Scorpaenidae

10.1007/978-94-007-6419-4_10

Cite

Citations (1)

Discovery of a Potent, Selective, and Blood-Brain Barrier Permeable Non-nitrocatechol Inhibitor of Catechol-O-methyltransferase

Journal of Medicinal Chemistry (2024)

Sofia Benfeito Bárbara Albuquerque Lisa Sequeira Carla Lima Daniel Chavarria

A new library of non-nitrocatechol compounds (HetCAMs) was developed and their efficacy was compared to tolcapone, a standard COMT inhibitor for PD. Compound

Catechol

10.1021/acs.jmedchem.4c01682

Cite

Citations (0)

Natterin-Induced Neutrophilia Is Dependent on cGAS/STING Activation via Type I IFN Signaling Pathway

International Journal of Molecular Sciences (2022)

Carla Lima Aline Ingrid Andrade‐Barros Jefferson Thiago Gonçalves Bernardo Enikő Balogh Valérie Quesniaux

Natterin is a potent pro-inflammatory fish molecule, inducing local and systemic IL-1β/IL-1R1-dependent neutrophilia mediated by non-canonical NLRP6 and NLRC4 inflammasome activation in mice, independent of NLRP3. In this work, we investigated whether Natterin activates mitochondrial damage, resulting in self-DNA leaks into the cytosol, and whether the DNA sensor cGAS and STING pathway participate in triggering the innate immune response. Employing a peritonitis mouse model, we found that the deficiency of the tlr2/tlr4, myd88 and trif results in decreased neutrophil influx to peritoneal cavities of mice, indicative that in addition to MyD88, TRIF contributes to neutrophilia triggered by TLR4 engagement by Natterin. Next, we demonstrated that gpcr91 deficiency in mice abolished the neutrophil recruitment after Natterin injection, but mice pre-treated with 2-deoxy-d-glucose that blocks glycolysis presented similar infiltration than WT Natterin-injected mice. In addition, we observed that, compared with the WT Natterin-injected mice, DPI and cyclosporin A treated mice had a lower number of neutrophils in the peritoneal exudate. The levels of dsDNA in the supernatant of the peritoneal exudate and processed IL-33 in the supernatant of the peritoneal exudate or cytoplasmic supernatant of the peritoneal cell lysate of WT Natterin-injected mice were several folds higher than those of the control mice. The recruitment of neutrophils to peritoneal cavity 2 h post-Natterin injection was intensely impaired in ifnar KO mice and partially in il-28r KO mice, but not in ifnγr KO mice. Finally, using cgas KO, sting KO, or irf3 KO mice we found that recruitment of neutrophils to peritoneal cavities was virtually abolished in response to Natterin. These findings reveal cytosolic DNA sensors as critical regulators for Natterin-induced neutrophilia.

Neutrophilia

TRIF

Peritoneal cavity

Infiltration (HVAC)

10.3390/ijms23073600

Cite

Citations (6)

Revisiting Retinal Degeneration Hallmarks: Insights from Molecular Markers and Therapy Perspectives

International Journal of Molecular Sciences (2023)

João Gabriel Santos Rosa Geonildo Rodrigo Disner Felipe Justiniano Pinto Carla Lima Mônica Lopes‐Ferreira

Visual impairment and blindness are a growing public health problem as they reduce the life quality of millions of people. The management and treatment of these diseases represent scientific and therapeutic challenges because different cellular and molecular actors involved in the pathophysiology are still being identified. Visual system components, particularly retinal cells, are extremely sensitive to genetic or metabolic alterations, and immune responses activated by local insults contribute to biological events, culminating in vision loss and irreversible blindness. Several ocular diseases are linked to retinal cell loss, and some of them, such as retinitis pigmentosa, age-related macular degeneration, glaucoma, and diabetic retinopathy, are characterized by pathophysiological hallmarks that represent possibilities to study and develop novel treatments for retinal cell degeneration. Here, we present a compilation of revisited information on retinal degeneration, including pathophysiological and molecular features and biochemical hallmarks, and possible research directions for novel treatments to assist as a guide for innovative research. The knowledge expansion upon the mechanistic bases of the pathobiology of eye diseases, including information on complex interactions of genetic predisposition, chronic inflammation, and environmental and aging-related factors, will prompt the identification of new therapeutic strategies.

Degeneration (medical)

10.3390/ijms241713079

Cite

Citations (7)