Yujin Kang

Guangxi University

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Ja Yil Lee

Sungkyunkwan University

AeRi Kim

Pusan National University

Jin Gu Kang

Korea Institute of Science and Technology

Yea Woon Kim

Pusan National University

Jae Hyung Park

Sungkyunkwan University

Seok‐In Na

Jeonbuk National University

Dong-Suk Han

Hanyang University

Jong‐Wan Park

Seoul National University

Juyun Park

Pukyong National University

Hyun‐Jung Lee

Yonsei University

Cooperative Institutions

Synthetic Biologics (United States)

Ulsan National Institute of Science and Technology

Yonsei University

Institute for Basic Science

Hanyang University

Seoul National University

Korea Advanced Institute of Science and Technology

Pusan National University

Sungkyunkwan University

Jeonbuk National University

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JoVE Video Dataset

Yujin Kang Subin Bae Soyeong An Ja Yil Lee

Chromatin is a higher-order structure that packages eukaryotic DNA. Chromatin undergoes dynamic alterations according to the cell cycle phase and in response to environmental stimuli. These changes are essential for genomic integrity, epigenetic regulation, and DNA metabolic reactions such as replication, transcription, and repair. Chromatin assembly is crucial for chromatin dynamics and is catalyzed by histone chaperones. Despite extensive studies, the mechanisms by which histone chaperones enable chromatin assembly remains elusive. Moreover, the global features of nucleosomes organized by histone chaperones are poorly understood. To address these problems, this work describes a unique single-molecule imaging technique named DNA curtain, which facilitates the investigation of the molecular details of nucleosome assembly by histone chaperones. DNA curtain is a hybrid technique that combines lipid fluidity, microfluidics, and total internal reflection fluorescence microscopy (TIRFM) to provide a universal platform for real-time imaging of diverse protein-DNA interactions.Using DNA curtain, the histone chaperone function of Abo1, the Schizosaccharomyces pombe bromodomain-containing AAA+ ATPase, is investigated, and the molecular mechanism underlying histone assembly of Abo1 is revealed. DNA curtain provides a unique approach for studying chromatin dynamics.

Histone code

Epigenomics

Histone Methylation

10.3791/63501-v

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A Study of High-Pressure Gasoline Spray Added Biodiesel 5% in a Constant Volume Combustion Chamber

Energy Procedia (2019)

Sakda Thongchai Yujin Kang Ocktaeck Lim

This paper focuses on the gasoline spray characteristics added biodiesel 5% to apply in gasoline compression ignition engines. Spray macroscopic visualization was performed by planar laser-induced fluorescence (PLIF) technique. The spray characteristics were, therefore, conducted by varying high injection pressures with the common rail diesel system of a diesel engine and controlling ambient pressure (back pressure) by filling nitrogen gas in the constant volume combustion chamber (CVCC). The test fuel, gasoline blended with biodiesel 5% (GB05), and conventional diesel as the reference were performed to compare its behavior. Chemical and physical properties of the GB05, gasoline, and biodiesel including viscosity, density, lubricity, cloud and pour point were measured. A high-speed video camera was employed to record the spray pattern with frame speed 10,000 f/s. The spray penetration length and cone angle were measured and analyzed by image processing. Structure and density profiles were analyzed. As expected, 5% concentration of biodiesel significantly affected to enhance lubricity property of the GB. With small biodiesel concentration, the lubricity improves. When injection pressure was increased, the spray-penetration length was increased whereas the spray cone angle was slightly decreased. Under higher injection pressure, the turbulent structures are remarkable.

Lubricity

Spray characteristics

Common rail

10.1016/j.egypro.2019.01.162

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Single-molecule fluorescence imaging techniques reveal molecular mechanisms underlying deoxyribonucleic acid damage repair

Frontiers in Bioengineering and Biotechnology (2022)

Yujin Kang Soyeong An Duyoung Min Ja Yil Lee

Advances in single-molecule techniques have uncovered numerous biological secrets that cannot be disclosed by traditional methods. Among a variety of single-molecule methods, single-molecule fluorescence imaging techniques enable real-time visualization of biomolecular interactions and have allowed the accumulation of convincing evidence. These techniques have been broadly utilized for studying DNA metabolic events such as replication, transcription, and DNA repair, which are fundamental biological reactions. In particular, DNA repair has received much attention because it maintains genomic integrity and is associated with diverse human diseases. In this review, we introduce representative single-molecule fluorescence imaging techniques and survey how each technique has been employed for investigating the detailed mechanisms underlying DNA repair pathways. In addition, we briefly show how live-cell imaging at the single-molecule level contributes to understanding DNA repair processes inside cells.

Fluorescence-lifetime imaging microscopy

Single-molecule experiment

10.3389/fbioe.2022.973314

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Efficient Slot-Die-Processed Perovskite Solar Cells With 3-Allyloxy-1, 2-Propanediol Additive

IEEE Journal of Photovoltaics (2022)

Hyun‐Jung Lee Yujin Kang Sung‐Nam Kwon Seok‐In Na

A scalable and spin-coating-free perovskite production method to guarantee highly efficient photovoltaic devices is still challenging. In this article, we studied a functional additive-assisted slot-die-coating process for better perovskite fabrication. We newly utilize 3-allyloxy-1, 2-propanediol (AP) as an additive in perovskite precursor solutions for slot-die-coating-based perovskite solar cells (S-PeSCs). The hydroxyl groups of the AP interacted with the perovskite, leading to the formation of a high-quality slot-die coated film, the suppression of perovskite surface defects, and thus improving S-PeSCs performance and stability. As a result, AP-treated slot-die-coating PeSCs showed 15.63%. In addition, AP-treated PeSC provided excellent stability, maintaining 70% of the initial efficiency during 151 d.

Spin Coating

10.1109/jphotov.2022.3143463

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Effects of zirconium doping on the characteristics of tin oxide thin film transistors

Microelectronics Reliability (2013)

Dong-Suk Han Jae Hyung Park Yujin Kang Jong‐Wan Park

Tin oxide

10.1016/j.microrel.2013.07.001

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Citations (23)

Erythroid specific activator GATA-1-dependent interactions between CTCF sites around the β-globin locus

Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms (2017)

Yujin Kang Yea Woon Kim Jin Gu Kang Won Ju Yun AeRi Kim

CTCF

Locus control region

10.1016/j.bbagrm.2017.01.013

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Hybrid Electrolytes Using Covalent Organic Frameworks (COFs) as a Filler for All-Solid-State Batteries

Journal of the Korean Battery Society (2024)

Da-eun Han Yujin Kang Heon‐Jin Choi Mihye Wu

Filler (materials)

10.53619/kobs.2024.6.4.1.47

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Alteration of replication protein A binding mode on single-stranded DNA by NSMF potentiates RPA phosphorylation by ATR kinase

Nucleic Acids Research (2023)

Yujin Kang Ye Gi Han Keon Woo Khim Woo Gyun Choi Min Kyung Ju

Replication protein A (RPA), a eukaryotic single-stranded DNA (ssDNA) binding protein, dynamically interacts with ssDNA in different binding modes and plays essential roles in DNA metabolism such as replication, repair, and recombination. RPA accumulation on ssDNA due to replication stress triggers the DNA damage response (DDR) by activating the ataxia telangiectasia and RAD3-related (ATR) kinase, which phosphorylates itself and downstream DDR factors, including RPA. We recently reported that the N-methyl-D-aspartate receptor synaptonuclear signaling and neuronal migration factor (NSMF), a neuronal protein associated with Kallmann syndrome, promotes RPA32 phosphorylation via ATR upon replication stress. However, how NSMF enhances ATR-mediated RPA32 phosphorylation remains elusive. Here, we demonstrate that NSMF colocalizes and physically interacts with RPA at DNA damage sites in vivo and in vitro. Using purified RPA and NSMF in biochemical and single-molecule assays, we find that NSMF selectively displaces RPA in the more weakly bound 8- and 20-nucleotide binding modes from ssDNA, allowing the retention of more stable RPA molecules in the 30-nt binding mode. The 30-nt binding mode of RPA enhances RPA32 phosphorylation by ATR, and phosphorylated RPA becomes stabilized on ssDNA. Our findings provide new mechanistic insight into how NSMF facilitates the role of RPA in the ATR pathway.

Replication protein A

10.1093/nar/gkad543

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Citations (10)

Review of Carbon Materials Used in Fuel cell Components

Journal of the Korean Chemical Society (2021)

Min-Hyeok Jang Yujin Kang Hyung-Kun Jo Cho-I Park Hye-Soo Sim

Carbon fibers

10.15207/jkcs.2021.12.2.193

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Vector Quantization for Deep-Learning-Based CSI Feedback in Massive MIMO Systems