The generation of the tolerogenic dendritic cells (DC) is not fully understood yet. Forkhead box protein-3 (Foxp3) is an important molecule in the immune tolerance. This study tests a hypothesis that DCs express Foxp3, which can be upregulated by Staphylococcal enterotoxin B (SEB).The expression of Foxp3 by DCs was evaluated by real-time RT-PCR, Western blotting, flow cytometry, and chromatin immunoprecipitation assay.We observed that mice treated with SEB at 0.25-0.5 μg/mouse showed high frequencies of transforming growth factor (TGF)-β-producing CD4+ T cells and TGF-β-producing DCs in the intestine, while the IL-4+ CD4+ T cells and TIM4+ DCs were dominated in the intestine in mice treated with SEB at 1-10 μg/mouse. Treating DCs with SEB in the culture induced high levels of Foxp3 at the TGF-β promoter locus. The function of Foxp3 was blocked by STAT6 (signal transducer and activator transcription-6); the latter was induced by exposing DCs to SEB in the culture at doses of 100-400 ng/ml. Treating allergic mice with specific immunotherapy (SIT) together with SEB significantly promoted the therapeutic effects on the allergic responses than treating with SIT alone.Dendritic cells have the capacity to express Foxp3, which can be upregulated by exposure to SEB.
[Objective] To investigate effect of RHIZOMA CHUANXIONG on the myocardial contractility and heart rate of toad.[Method] The toads were given 0.01,0.0 and 0.05 g/ml RHIZOMA CHUANXIONG solution respectively;in addition,the toads were given 0.04 g/ml of RHIZOMA CHUANXIONG solution after infusion of atropine,investigating effect of RHIZOMA CHUANXIONG on the myocardial contractility and heart rate of toad.[Result] Effect of RHIZOMA CHUANXIONG solution of 0.02 and 0.05 g/ml on myocardial contractility and heart rate of toad was significant(P0.01),which was different from that of 0.01 g/ml.When given atropine firstly,toad given 0.04 g/ml of RHIZOMA CHUANXIONG solution was not decreased in myocardial contractility.[Conclusion] RHIZOMA CHUANXIONG solution could reduce the myocardium of toad heart,the mechanism of which might be concerned with M receptor.
Abstract Hyperglycemia and its induced oxidative stress microenvironment present formidable challenges to the repair of diabetic bone defects. Herein, a novel TZGP (α‐TCP/ZnO/GM@P2) composite scaffold for diabetic bone defect repair is developed. Characterization results demonstrate that the α‐TCP cement scaffold, ZnO nanoparticles, and gelatin microspheres loaded with and protecting P2 (a novel parathyroid hormone‐related peptide) achieve complementary advantages. The TZGP scaffold, while meeting the mechanical strength requirements of cancellous bone, compensates for the deficiency of bioactive peptides in inorganic scaffolds. Its biocompatibility is enhanced, and the scaffold is endowed with antibacterial and antioxidant properties. The in vitro and in vivo results reveal that the TZGP scaffold releases active factors P2 and Zn 2+ , promotes cell proliferation and recruitment in a high‐glucose microenvironment, reduces cellular reactive oxygen species accumulation, improves DNA injury and mitochondrial homeostasis, and induces osteogenic‐angiogenic differentiation. Compared with the conventional α‐TCP scaffold, the TZGP scaffold exhibits more excellent biodegradability, accelerates local tissue filling, and promotes bone accumulation and vascular reconstruction in diabetic bone defects. Consequently, the novel design strategy of the TZGP scaffold offers a promising approach to repairing diabetic bone defects.
According to the readjustment of national industrial structure, the demand for talents on mainframe and strong profession in mainframe applications, Dalian Jiaotong University, taking advantage of our own Cultivation, combines mainframe technology with our school compound dual professional software talents training model. Meanwhile, with an integrated curriculum teaching methods and integration process and associating with relevant courses, we build a mode of curriculum chain so that students can master the knowledge about relationships among all courses, and solve synthesis problems. We focus on applying structures "Platform + Module" in the course structure, designing rational course modules, and strengthening capacity-building. Meanwhile, we have long-term cooperation with IBM and others' mainframe industry's well-known enterprises at home and abroad, and invite teachers with background of engineering practice into the classroom, and build a practice base with company. With the purpose of enhancing students engineering training and designing capacity-building, we focus on building teaching and training system of "learning by doing" that requires engineering practice background in engineering compound mainframe software talents. It has been proved that this new compound mainframe training mode can meet the requirement of social development, and continuously improve the school's quality and effectively enhance students' practical applications ability.
To characterize an MDR blaIMP-4-harbouring plasmid from Enterobacter cloacae EC62 of swine origin in China. Plasmid pIMP-4-EC62 from E. cloacae EC62 was transferred by conjugation via filter mating into Escherichia coli J53. Plasmid DNA was extracted from an E. coli J53 transconjugant and sequenced using single-molecule real-time (SMRT) technology. MIC values for both the isolate EC62 and the transconjugant were determined using the broth microdilution and agar dilution methods. Plasmid stability in both the isolate EC62 and the transconjugant was assessed through a series of passages on antibiotic-free media. Plasmid pIMP-4-EC62 is 314351 bp in length, encodes 369 predicted proteins and harbours a novel class 1 integron carrying blaIMP-4 and a group II intron. The blaIMP-4-bearing plasmid belongs to the IncHI2/ST1 incompatibility group. Sequence analysis showed that pIMP-4-EC62 carries four MDR regions and several gene clusters encoding heavy metal resistance. Plasmid pIMP-4-EC62 was stably maintained in both the E. cloacae EC62 isolate and the transconjugant E. coli J53-pIMP-4-EC62 in the absence of selective pressure. Analysis of the evolutionary relatedness of selected IncHI2 plasmids indicates that ST1-type plasmids are key carriers of carbapenemase genes among IncHI2 plasmids. pIMP-4-EC62 represents the first fully sequenced IncHI2-type blaIMP-4-harbouring plasmid from E. cloacae in China. Co-location of blaIMP-4 with other resistance genes on an MDR plasmid is likely to further accelerate the dissemination of blaIMP-4 by co-selection among bacteria from humans, animals and the environment under the selective pressure of other antimicrobial agents, heavy metals and disinfectants.
To study the effect of variant administration and cumulative dose of glucocorticoid on the skeleton, thirty male rabbits were divided into 5 groups, serum calcium, phosphirus, alkaline phosphatase and N terminalfragment of parathyroid hormone (PTH) and bone mineral content (BMC) were determined, The results showed no significant differ ences were observed between supplerment calcium group and control group at the level of BMC and PTH (P0 05) , but there were significantly decreased BMC and elevated PTH in other groups (P0 01) , especially in the high dose group. The results indicated that glucocorticoid induced osteoporosis is associated with the steroid dose, and the pathogenesis is concerned with the development of secondary hyperparathyroidism. Alternate day therapy cant prevent bone loss. Supplementation of calcium and vitamin D is an effective method for the prevention and treatment.
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