Alexandra Cieslak

Institut de Biologia Evolutiva

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Ignacio Ribera

Institut de Biologia Evolutiva

Javier Fresneda

Universitat de Lleida

David Sánchez‐Fernández

Universidad de Murcia

Amparo Hidalgo‐Galiana

Institut de Biologia Evolutiva

Valeria Rizzo

Institut de Biologia Evolutiva

Wolf‐Georg Forssmann

Pharis Biotec (Germany)

Arnaud Faille

Staatliches Museum für Naturkunde Stuttgart

Hans‐Jürgen Mägert

Anhalt University of Applied Sciences

Alfried P. Vogler

Natural History Museum

Carles Hernando

Natural Science Museum of Barcelona

Cooperative Institutions

Medizinische Hochschule Hannover

Institut de Biologia Evolutiva

Natural Science Museum of Barcelona

University of Castilla-La Mancha

Universidad de Murcia

Pompeu Fabra University

Universidad de Sevilla

Universitat Politècnica de Catalunya

Barcelona Supercomputing Center

Centre National de la Recherche Scientifique

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A new human guanylate cyclase-activating peptide (GCAP-II, uroguanylin): precursor cDNA and colonic expression

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics (1995)

Oliver Hill Yalcin Cetin Alexandra Cieslak Hans‐Jürgen Mägert Wolf‐Georg Forssmann

Northern blot

10.1016/0167-4838(95)00204-4

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Citations (47)

Aplicaciones de proteómica en ecología y evolución

Ecosistemas: Revista científica y técnica de ecología y medio ambiente (2009)

Alexandra Cieslak Ignacio Ribera

Cieslak, A., Ribera, I. (2009). Applications of proteomics in ecology and evolution. Ecosistemas 18(1):34-43. The evolution of ecological traits has been subject to intense research efforts for a long time. The complexity of ecological traits usually limits these studies to indirect evidence on the actual adaptive variations. More recently, molecular methods, such as micro-arrays and large-scale genomic sequencing, considerably increased the knowledge on genomes and metabolic pathways. With this, it became possible for the first time to study in detail the interaction of organisms and their environment at the molecular level. However, the investigation of biochemical processes associated to ecological and evolutionary questions relating organisms to their habitats are typically restricted to so-called model organisms. The latest methodological advances in proteomics led to a strongly increase in their use in ecology and evolutionary biology. These approaches are in many respects suitable for ecological studies, and offer the possibility to work on a wide range of organisms. Here we briefly review the methodology and describe how proteomics has been used to date in different fields of evolutionary biology and ecology in non-model organisms.

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Filogeografía del grupo Agabus brunneus (Dytiscidae: Coleoptera): evaluando/resolviendo una especiación reciente

Amparo Hidalgo‐Galiana Alexandra Cieslak Ignacio Ribera

Trabajo presentado en el I Congreso Iberico de Sistematica Animal (CISA2012), celebrado en Madrid del 17 al 19 de enero de 2012.

Dytiscidae

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Gene expression of aphrodisin in female hamster genital tract segments

Cell and Tissue Research (1996)

Mogens Kruhøffer Achim Bub Alexandra Cieslak Knut Adermann I. Kunstýř

Golden hamster

10.1007/s004410050741

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Investigation of hormone activity in butterfly imaginal wing discs by protein expression pattern changes

Electrophoresis (2007)

Alexandra Cieslak Alfried P. Vogler Nathalie J. Lafranchi-Tristem Michael J. Dünn Jules A. Westbrook

In a proteomic approach using 2-DE, the changes in protein expression patterns in wing imaginal discs induced by hormone treatment have been studied. Here we show the response of butterfly imaginal wing disc tissue taken from late fifth instar larvae of the African-Mocker swallowtail Papilio dardanus (Lepidoptera) to the insect hormones 20-hydroxyecdysone (20-HE) and juvenile hormone (JH). The tissues were cultured in the presence of one hormone or a combination of both and their protein expression was compared to the pattern obtained from untreated wing discs. All the treatments resulted in changes in the expression pattern distinct from the uninduced control, indicating a distinct protein regulation induced by the hormones. The treatment with both of the hormones, which are known to have antagonistic physiological effects, did show a unique pattern, presumably the result, in part, of synergistic effects on protein expression mediated by the combined effects of both the hormones. The extent of the interaction between JH and 20-HE indicates a complex molecular regulation, far beyond a simple antagonistic effect.

Expression (computer science)

10.1002/elps.200600620

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Multi-stage analysis of differential gene expression in BALB/C mouse liver development by high-density microarrays

Differentiation (2003)

Andrea Jochheim Alexandra Cieslak Tina Hillemann Tobias Cantz Jennifer Scharf

10.1046/j.1432-0436.2003.700606.x

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Citations (45)

Thermal niche evolution and geographical range expansion in a species complex of western Mediterranean diving beetles

BMC Evolutionary Biology (2014)

Amparo Hidalgo‐Galiana David Sánchez‐Fernández David T. Bilton Alexandra Cieslak Ignacio Ribera

Species thermal requirements are one of the principal determinants of their ecology and biogeography, although our understanding of the interplay between these factors is limited by the paucity of integrative empirical studies. Here we use empirically collected thermal tolerance data in combination with molecular phylogenetics/phylogeography and ecological niche modelling to study the evolution of a clade of three western Mediterranean diving beetles, the Agabus brunneus complex. The preferred mitochondrial DNA topology recovered A. ramblae (North Africa, east Iberia and Balearic islands) as paraphyletic, with A. brunneus (widespread in the southwestern Mediterranean) and A. rufulus (Corsica and Sardinia) nested within it, with an estimated origin between 0.60-0.25 Ma. All three species were, however, recovered as monophyletic using nuclear DNA markers. A Bayesian skyline plot suggested demographic expansion in the clade at the onset of the last glacial cycle. The species thermal tolerances differ significantly, with A. brunneus able to tolerate lower temperatures than the other taxa. The climatic niche of the three species also differs, with A. ramblae occupying more arid and seasonal areas, with a higher minimum temperature in the coldest month. The estimated potential distribution for both A. brunneus and A. ramblae was most restricted in the last interglacial, becoming increasingly wider through the last glacial and the Holocene. The A. brunneus complex diversified in the late Pleistocene, most likely in south Iberia after colonization from Morocco. Insular forms did not differentiate substantially in morphology or ecology, but A. brunneus evolved a wider tolerance to cold, which appeared to have facilitated its geographic expansion. Both A. brunneus and A. ramblae expanded their ranges during the last glacial, although they have not occupied areas beyond their LGM potential distribution except for isolated populations of A. brunneus in France and England. On the islands and possibly Tunisia secondary contact between A. brunneus and A. ramblae or A. rufulus has resulted in introgression. Our work highlights the complex dynamics of speciation and range expansions within southern areas during the last glacial cycle, and points to the often neglected role of North Africa as a source of European biodiversity.

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Paraphyly

10.1186/s12862-014-0187-y

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Survey on Usage of 5G Campus Networks in Intralogistics

Lecture notes in networks and systems (2022)

Lara Nehrke Simone Neumann Alexandra Cieslak Alice Kirchheim

Questionnaire

10.1007/978-3-031-16281-7_45

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Ancient origin of a Western Mediterranean radiation of subterranean beetles

BMC Evolutionary Biology (2010)

Ignacio Ribera Javier Fresneda Ruxandra Năstase-Bucur Ana Navas Alfried P. Vogler

Cave organisms have been used as models for evolution and biogeography, as their reduced above-ground dispersal produces phylogenetic patterns of area distribution that largely match the geological history of mountain ranges and cave habitats. Most current hypotheses assume that subterranean lineages arose recently from surface dwelling, dispersive close relatives, but for terrestrial organisms there is scant phylogenetic evidence to support this view. We study here with molecular methods the evolutionary history of a highly diverse assemblage of subterranean beetles in the tribe Leptodirini (Coleoptera, Leiodidae, Cholevinae) in the mountain systems of the Western Mediterranean. Ca. 3.5 KB of sequence information from five mitochondrial and two nuclear gene fragments was obtained for 57 species of Leptodirini and eight outgroups. Phylogenetic analysis was robust to changes in alignment and reconstruction method and revealed strongly supported clades, each of them restricted to a major mountain system in the Iberian peninsula. A molecular clock calibration of the tree using the separation of the Sardinian microplate (at 33 MY) established a rate of 2.0% divergence per MY for five mitochondrial genes (4% for cox1 alone) and dated the nodes separating the main subterranean lineages before the Early Oligocene. The colonisation of the Pyrenean chain, by a lineage not closely related to those found elsewhere in the Iberian peninsula, began soon after the subterranean habitat became available in the Early Oligocene, and progressed from the periphery to the centre. Our results suggest that by the Early-Mid Oligocene the main lineages of Western Mediterranean Leptodirini had developed all modifications to the subterranean life and were already present in the main geographical areas in which they are found today. The origin of the currently recognised genera can be dated to the Late Oligocene-Miocene, and their diversification can thus be traced to Miocene ancestors fully adapted to subterranean life, with no evidence of extinct epigean, less modified lineages. The close correspondence of organismal evolution and geological record confirms them as an important study system for historical biogeography and molecular evolution.

Entomology

10.1186/1471-2148-10-29

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Citations (166)

Reproducibility and Consistency of Proteomic Experiments on Natural Populations of a Non-Model Aquatic Insect

PLoS ONE (2014)

Amparo Hidalgo‐Galiana Marta Monge David G. Biron Françesc Canals Ignacio Ribera

Population proteomics has a great potential to address evolutionary and ecological questions, but its use in wild populations of non-model organisms is hampered by uncontrolled sources of variation. Here we compare the response to temperature extremes of two geographically distant populations of a diving beetle species (Agabus ramblae) using 2-D DIGE. After one week of acclimation in the laboratory under standard conditions, a third of the specimens of each population were placed at either 4 or 27°C for 12 h, with another third left as a control. We then compared the protein expression level of three replicated samples of 2-3 specimens for each treatment. Within each population, variation between replicated samples of the same treatment was always lower than variation between treatments, except for some control samples that retained a wider range of expression levels. The two populations had a similar response, without significant differences in the number of protein spots over- or under-expressed in the pairwise comparisons between treatments. We identified exemplary proteins among those differently expressed between treatments, which proved to be proteins known to be related to thermal response or stress. Overall, our results indicate that specimens collected in the wild are suitable for proteomic analyses, as the additional sources of variation were not enough to mask the consistency and reproducibility of the response to the temperature treatments.

10.1371/journal.pone.0104734

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Citations (10)