Marie Březinová

Czech Academy of Sciences, Institute of Physiology

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Ondřej Kuda

Czech Academy of Sciences, Institute of Physiology

Ján Kopecký

Czech Academy of Sciences

Laurence Balas

Université de Montpellier

Thierry Durand

Institut des Biomolécules Max Mousseron

Martin Rossmeisl

Czech Academy of Sciences

Kristýna Brejchová

Czech Academy of Sciences, Institute of Physiology

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Tomáš Čajka

Czech Academy of Sciences, Institute of Physiology

Kristina Bardová

Czech Academy of Sciences

Veronika Paluchová

Czech Academy of Sciences, Institute of Physiology

Eva Kudová

Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry

Cooperative Institutions

Charles University

Czech Academy of Sciences

Czech Academy of Sciences, Institute of Physiology

Czech Academy of Sciences, Institute of Molecular Genetics

Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry

General University Hospital in Prague

Institute of Clinical and Experimental Medicine

Sapienza University of Rome

Masaryk University

Czech University of Life Sciences Prague

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Reduced Number of Adipose Lineage and Endothelial Cells in Epididymal fat in Response to Omega-3 PUFA in Mice Fed High-Fat Diet

Marine Drugs (2018)

Kateřina Adamcová Olga Horakova Kristina Bardová Petra Janovská Marie Březinová

We found previously that white adipose tissue (WAT) hyperplasia in obese mice was limited by dietary omega-3 polyunsaturated fatty acids (omega-3 PUFA). Here we aimed to characterize the underlying mechanism. C57BL/6N mice were fed a high-fat diet supplemented or not with omega-3 PUFA for one week or eight weeks; mice fed a standard chow diet were also used. In epididymal WAT (eWAT), DNA content was quantified, immunohistochemical analysis was used to reveal the size of adipocytes and macrophage content, and lipidomic analysis and a gene expression screen were performed to assess inflammatory status. The stromal-vascular fraction of eWAT, which contained most of the eWAT cells, except for adipocytes, was characterized using flow cytometry. Omega-3 PUFA supplementation limited the high-fat diet-induced increase in eWAT weight, cell number (DNA content), inflammation, and adipocyte growth. eWAT hyperplasia was compromised due to the limited increase in the number of preadipocytes and a decrease in the number of endothelial cells. The number of leukocytes and macrophages was unaffected, but a shift in macrophage polarization towards a less inflammatory phenotype was observed. Our results document that the counteraction of eWAT hyperplasia by omega-3 PUFA in dietary-obese mice reflects an effect on the number of adipose lineage and endothelial cells.

Stromal vascular fraction

10.3390/md16120515

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Citations (17)

Metabolický syndrom - hrozba civilizačního onemocnění

Marie Březinová

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Levels of palmitic acid ester of hydroxystearic acid (PAHSA) are reduced in the breast milk of obese mothers

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids (2017)

Marie Březinová Ondřej Kuda Jana Hansíková Martina Rombaldová Laurence Balas

10.1016/j.bbalip.2017.11.004

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Lipokine 5-PAHSA Is Regulated by Adipose Triglyceride Lipase and Primes Adipocytes for De Novo Lipogenesis in Mice

Diabetes (2019)

Veronika Paluchová Marina Oseeva Marie Březinová Tomáš Čajka Kristina Bardová

Branched esters of palmitic acid and hydroxystearic acid (PAHSA) are anti-inflammatory and antidiabetic lipokines that connect glucose and lipid metabolism. We aimed to characterize involvement of the 5-PAHSA regioisomer in the adaptive metabolic response of white adipose tissue (WAT) to cold exposure (CE) in mice, exploring the cross talk between glucose utilization and lipid metabolism. CE promoted local production of 5- and 9-PAHSAs in WAT. Metabolic labeling of de novo lipogenesis (DNL) using 2H2O revealed that 5-PAHSA potentiated the effects of CE and stimulated triacylglycerol (TAG)/fatty acid (FA) cycling in WAT through impacting lipogenesis and lipolysis. Adipocyte lipolytic products were altered by 5-PAHSA through selective FA re-esterification. The impaired lipolysis in global adipose triglyceride lipase (ATGL) knockout mice reduced free PAHSA levels and uncovered a metabolite reservoir of TAG-bound PAHSAs (TAG estolides) in WAT. Utilization of 13C isotope tracers and dynamic metabolomics documented that 5-PAHSA primes adipocytes for glucose metabolism in a different way from insulin, promoting DNL and impeding TAG synthesis. In summary, our data reveal new cellular and physiological mechanisms underlying the beneficial effects of 5-PAHSA and its relation to insulin action in adipocytes and independently confirm a PAHSA metabolite reservoir linked to ATGL-mediated lipolysis.

Lipogenesis

Adipose triglyceride lipase

Lipid droplet

10.2337/db19-0494

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Hospodářský výsledek (zisk nebo ztráta), formy, způsob řešení dle vybrané právní formy

Marie Březinová

Cilem teto bakalařske prace je zjistěni výsledku hospodařeni a jeho způsobu řeseni ve spolecnosti Fortex ? AGS, a. s. v letech 2006 a 2008. V prvni casti jsou zahrnuty obecně definice pojmů, způsoby rozděleni zisku nebo ztraty podle pravnich forem a poměrove ukazatele. Prakticka cast obsahuje profil a charakteristiku spolecnosti. Dale se věnuje hospodařskemu výsledku, tj. jak jej vypocitame, co jej tvoři a co z něj lze vycist. Ke konci prace je uvedeno rozděleni výsledku hospodařeni spolecnosti ve sledovaných letech.

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Triacylglycerol‐Rich Oils of Marine Origin are Optimal Nutrients for Induction of Polyunsaturated Docosahexaenoic Acid Ester of Hydroxy Linoleic Acid (13‐DHAHLA) with Anti‐Inflammatory Properties in Mice

Molecular Nutrition & Food Research (2020)

Veronika Paluchová Anders Vik Tomáš Čajka Marie Březinová Kristýna Brejchová

The docosahexaenoic acid ester of hydroxy linoleic acid (13-DHAHLA) is a bioactive lipid with anti-inflammatory properties from the family of fatty acid esters of hydroxy fatty acids (FAHFA).To explore the biosynthesis of 13-DHAHLA from dietary oils, C57BL/6N mice are gavaged for 8 days with various corn oil/marine oil mixtures containing the same amount of DHA. Plasma levels of omega-3 FAHFAs are influenced by the lipid composition of the mixtures but do not reflect the changes in bioavailability of polyunsaturated fatty acids in plasma. Triacylglycerol-bound DHA and linoleic acid serve as more effective precursors for 13-DHAHLA synthesis than DHA bound in phospholipids or wax esters. Both 13(S)- and 13(R)-DHAHLA inhibit antigen and PGE2 -induced chemotaxis and degranulation of mast cells to a comparable extent and 13(S)-DHAHLA is identified as the predominant isomer in mouse adipose tissue.Here, the optimal nutritional source of DHA is identified, which supports production of anti-inflammatory FAHFAs, as triacylglycerol-based marine oil and also reveals a possible role of triacylglycerols in the synthesis of FAHFA lipokines.

10.1002/mnfr.201901238

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Understanding FAHFAs: From structure to metabolic regulation

Progress in Lipid Research (2020)

Kristýna Brejchová Laurence Balas Veronika Paluchová Marie Březinová Thierry Durand

10.1016/j.plipres.2020.101053

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Citations (95)

Triacylglycerols containing branched palmitic acid ester of hydroxystearic acid (PAHSA) are present in the breast milk and hydrolyzed by carboxyl ester lipase

Food Chemistry (2022)

Kristýna Brejchová Veronika Paluchová Marie Březinová Tomáš Čajka Laurence Balas

10.1016/j.foodchem.2022.132983

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Citations (12)

Erratum. Docosahexaenoic Acid–Derived Fatty Acid Esters of Hydroxy Fatty Acids (FAHFAs) With Anti-inflammatory Properties. Diabetes 2016;65:2580–2590

Diabetes (2016)

Ondřej Kuda Marie Březinová Martina Rombaldová Barbora Slavı́ková Martin Pošta

In the article cited listed above, the grant number from the Czech Science Foundation was listed incorrectly and …

10.2337/db16-er11b

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Nrf2-Mediated Antioxidant Defense and Peroxiredoxin 6 Are Linked to Biosynthesis of Palmitic Acid Ester of 9-Hydroxystearic Acid

Diabetes (2018)

Ondřej Kuda Marie Březinová Jan Šilhavý Vladimír Landa Václav Zı́dek

Fatty acid esters of hydroxy fatty acids (FAHFAs) are lipid mediators with promising antidiabetic and anti-inflammatory properties that are formed in white adipose tissue (WAT) via de novo lipogenesis, but their biosynthetic enzymes are unknown. Using a combination of lipidomics in WAT, quantitative trait locus mapping, and correlation analyses in rat BXH/HXB recombinant inbred strains, as well as response to oxidative stress in murine models, we elucidated the potential pathway of biosynthesis of several FAHFAs. Comprehensive analysis of WAT samples identified ∼160 regioisomers, documenting the complexity of this lipid class. The linkage analysis highlighted several members of the nuclear factor, erythroid 2 like 2 (Nrf2)-mediated antioxidant defense system (Prdx6, Mgst1, Mgst3), lipid-handling proteins (Cd36, Scd6, Acnat1, Acnat2, Baat), and the family of flavin containing monooxygenases (Fmo) as the positional candidate genes. Transgenic expression of Nrf2 and deletion of Prdx6 genes resulted in reduction of palmitic acid ester of 9-hydroxystearic acid (9-PAHSA) and 11-PAHSA levels, while oxidative stress induced by an inhibitor of glutathione synthesis increased PAHSA levels nonspecifically. Our results indicate that the synthesis of FAHFAs via carbohydrate-responsive element-binding protein–driven de novo lipogenesis depends on the adaptive antioxidant system and suggest that FAHFAs may link activity of this system with insulin sensitivity in peripheral tissues.

10.2337/db17-1087

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Citations (64)