Ute Messelhäußer

Bayerisches Landesamt für Gesundheit und Lebensmittelsicherheit

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Ulrich Busch

Leibniz-Centre General Linguistics

Stefan Hörmansdorfer

Bayerisches Landesamt für Gesundheit und Lebensmittelsicherheit

Christiane Höller

Bayerisches Landesamt für Gesundheit und Lebensmittelsicherheit

Monika Ehling‐Schulz

University of Veterinary Medicine Vienna

Andreas Sing

Bayerisches Landesamt für Gesundheit und Lebensmittelsicherheit

Annemarie Käsbohrer

University of Veterinary Medicine Vienna

Ingrid Huber

Bayerisches Landesamt für Gesundheit und Lebensmittelsicherheit

A. Schmid

Ludwig-Maximilians-Universität München

Albert Rampp

Bundeswehr Medical Service Academy

D. Thärigen

Cooperative Institutions

Bayerisches Landesamt für Gesundheit und Lebensmittelsicherheit

Robert Koch Institute

Ludwig-Maximilians-Universität München

Federal Institute for Risk Assessment

Freie Universität Berlin

University of Veterinary Medicine Hannover, Foundation

Technical University of Munich

Institute of Bioinformatics and Systems Biology

Health and Safety Authority

University of Veterinary Medicine Vienna

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Occurrence of Zoonotic Clostridia and Yersinia in Healthy Cattle

Journal of Food Protection (2013)

A. Schmid Ute Messelhäußer Stefan Hörmansdorfer Carola Sauter‐Louis R. Mansfeld

Yersinia enterocolitica

Beef Cattle

Clostridia

Clostridium botulinum

10.4315/0362-028x.jfp-13-151

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Citations (14)

Between standardization and innovation. Monitoring of foods for pathogens.

Ute Messelhäußer Ulrich Busch

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Presence of virulence genes, adhesion and invasion ofArcobacter butzleri

Journal of Applied Microbiology (2013)

Gül Karadas Soroush Sharbati Ingrid Hänel Ute Messelhäußer Erik‐Oliver Glocker

Aims The pathogenic potential of Arcobacter butzleri isolates was investigated by detecting the presence of putative virulence genes and analysing the adhesive and invasive capabilities in cell cultures of human cell lines. Methods and Results The presence of ten putative virulence genes in 52 A. butzleri isolates was determined by PCR. The genes ciaB, mviN, pldA, tlyA, cj1349 and cadF were detected in all, whilst irgA (15%), iroE (60%), hecB (44%) and hecA (13%) were detected only in few A. butzleri isolates. On HT-29 cells, four of six isolates adhered to and three of them were able to invade, whilst all six isolates adhered to and invaded Caco-2 cells with higher degrees. The genes ciaB, cadF and cj1349 of all six isolates were sequenced, but no considerable changes of the amino acids in putative functional domains were observed. Conclusion Selected A. butzleri isolates adhere to and invade HT-29 and Caco-2 cells, which emphasize their human pathogenic potential. The efficiency of invasion depends on the eukaryotic cell line and individual bacterial strain used. We could not show any functional correlation between the amino acid sequence of CadF, CiaB or Cj1349 and the adhesive and invasive phenotype. Significance and Impact of the Study We have shown that some A. butzleri strains invade various cell lines. This underlines their pathogenic potential and hints at their relevance in human disease.

Arcobacter

Strain (injury)

10.1111/jam.12245

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Citations (77)

Hygiene and Sanitation in Biogas Plants

Advances in biochemical engineering, biotechnology (2015)

Bianca Fröschle Monika Heiermann Michael Lebuhn Ute Messelhäußer Matthias Plöchl

Digestate

Biogas

10.1007/978-3-319-21993-6_3

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Citations (29)

Detection, differentiation, and identification of botulinum neurotoxin serotypes C, CD, D, and DC by highly specific immunoassays and mass spectrometry

The Analyst (2016)

Eva-Maria Hansbauer Martin Skiba Tanja Endermann Jasmin Weisemann Daniel Stern

Botulinum neurotoxin (BoNT) serotypes C and D and their mosaic variants CD and DC cause severe cases of botulism in animal husbandry and wildlife. Epidemiological data on the exact serotype or toxin variant causing outbreaks are rarely available, mainly because of their high sequence identity and the lack of fast and specific screening tools to detect and differentiate the four similar toxins. To fill this gap, we developed four highly specific sandwich enzyme-linked immunosorbent assays (ELISAs) able to detect and differentiate botulinum neurotoxins type BoNT/C, D, CD, and DC based on four distinct combinations of specific monoclonal antibodies targeting both conserved and divergent subdomains of the four toxins. Here, highly sensitive detection with detection limits between 2 and 24 pg mL(-1) was achieved. The ELISAs were extensively validated and results were compared with data obtained by quantitative real-time PCR using a panel of Clostridium botulinum strains, real sample materials from veterinary botulism outbreaks, and non-BoNT-producing Clostridia. Additionally, in order to verify the results obtained by ELISA screening, the new monoclonal antibodies were used for BoNT enrichment and subsequent detection (i) on a functional level by endopeptidase mass spectrometry (Endopep-MS) assays and (ii) on a protein sequence level by LC-MS/MS spectrometry. Based on all technical information gathered in the validation study, the four differentiating ELISAs turned out to be highly reliable screening tools for the rapid analysis of veterinary botulism cases and should aid future field investigations of botulism outbreaks and the acquisition of epidemiological data.

Botulism

Clostridium botulinum

Botulinum neurotoxin

Endopeptidase

Multiplex

10.1039/c6an00693k

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Citations (22)

[Cultural detection of thermotolerant Campylobacter spp. in food--potentials and limitations of diagnostic tools in the context of official food control].

PubMed (2015)

Ute Messelhäußer D. Thärigen Christiane Fella Hermann Schreiner Ulrich Busch

Thermotolerant Campylobacter spp. rank among the most important foodborne pathogens in Germany. Therefore a necessity for rapid and routinely useable detection methods exists also in the area of food microbiology. A reliable, cultura qualitative, but also quantitative detection of thermotolerant Campylobacter spp. pose a challenge, at least concerning special food matrices, especially because in the context of official food control the cultural detection of thermotolerant Campylobacter spp. is needed. This was the reason, why different cultural detection methods, beside the standard procedure of ISO 10272:2006, in combination with molecular and immunological screening methods were tested at the Bavarian Health and Food Safety Authority (LGL) during the last years for the use in routine diagnostic using different food matrices of animal and plant origin. The results of the comparative studies showed clearly that no enrichment broth tested gave completely satisfactory results for an only culture-based detection the combination with a screening method is therefore recommended for a rapid and reliable detection. But in this case the user should take into account that the sensitivity of such molecular and immunological methods is normally so high that in some cases, depending on the food matrix and processing step, the isolation of the pathogen would not be possible in samples, which were positive in the screening methods.

Isolation

Foodborne pathogen

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Lebensmittelbedingte Übertragung von Tuberkulose – ein vergessener Infektionsweg?

Krankenhaushygiene up2date (2014)

Ute Messelhäußer Albert Rampp

Kernaussagen Die Tuberkulose gehörte zu den ersten Zoonosen, die durch repressive staatliche Maßnahmen, beispielsweise durch Einführung des Pasteurisierungszwangs, und konsequente Bekämpfungsprogramme in den Tierbeständen im Bereich der Lebensmittelhygiene in Deutschland als „getilgt“ galten. Dass die Tuberkulose in den Tierbeständen nicht vollständig verschwunden ist und jederzeit wieder aufflackern kann, zeigen aktuelle Vorkommen. Solche Ereignisse sowie die fortschreitende Globalisierung mit Importen von Lebensmitteln auch aus Ländern, in denen Tuberkulose bei Haus- und Wildwiederkäuern endemisch vorkommen, führen dazu, dass man sich im Lebensmittelbereich wieder verstärkt mit dieser „Re-emerging Disease“ befassen muss. Nachdem ein Eintrag in die Lebensmittelkette nicht vollständig ausgeschlossen werden kann und geänderte Verzehrgewohnheiten in Deutschland zu einem verstärkten Konsum von rohen oder wenig prozessierten Lebensmitteln führen, sollte auch im Bereich der Humanmedizin an ein mögliches Auftreten lebensmittelbedingter Tuberkulosefälle gedacht werden. Es ist zu berücksichtigen, dass bei lebensmittelbedingten Tuberkulosefällen häufig nicht die klassische Symptomatik auftritt, sondern andere Organsysteme als die Lunge betroffen sind, wie z. B. der Gastrointestinaltrakt.

10.1055/s-0034-1377585

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Prevalence of Emetic Bacillus cereus in Different Ice Creams in Bavaria

Journal of Food Protection (2010)

Ute Messelhäußer Peter Kämpf Martina Fricker Monika Ehling‐Schulz R. Zucker

Cereus

Ice cream

10.4315/0362-028x-73.2.395

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Citations (35)

Erratum to “Detection and differentiation of Vibrio spp. in seafood and fish samples with cultural and molecular methods” [Int. J. of Food Microbiol. 142 (2010) 360–364]