Tiziana Parasassi

Istituto di Farmacologia Traslazionale

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Enrico Gratton

University of California, Irvine

Roberto Brunelli

Policlinico Umberto I

Marco De Spirito

Università Cattolica del Sacro Cuore

Fulvio Ursini

University of Padua

Ewa K. Krasnowska

National Research Council

Giampietro Ravagnan

Istituto di Farmacologia Traslazionale

Massimiliano Papi

Agostino Gemelli University Polyclinic

O. Sapora

Istituto Superiore di Sanità

Giulia Greco

University of Bologna

Giuseppe Maulucci

Università Cattolica del Sacro Cuore

Cooperative Institutions

Sapienza University of Rome

National Research Council

Policlinico Umberto I

University of Rome Tor Vergata

Università Cattolica del Sacro Cuore

Istituti di Ricovero e Cura a Carattere Scientifico

University of Padua

Agostino Gemelli University Polyclinic

Istituto Superiore di Sanità

National Academies of Sciences, Engineering, and Medicine

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Phase fluctuation in phospholipid membranes revealed by Laurdan fluorescence

Biophysical Journal (1990)

Tiziana Parasassi Gelsomina De Stasió A D'Ubaldo Enrico Gratton

Laurdan

10.1016/s0006-3495(90)82637-0

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Citations (756)

Influence of sphingomyelin on lipid dynamics as detected by Laurdan fluorescence.

Biophysical Journal (1997)

Kumar Paul D. Wilson Tiziana Parasassi Enrico Gratton Moshe Levi

Laurdan

Lipid microdomain

Lipid raft

Source

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Citations (0)

Hoechst 33258 Staining for Detecting Mycoplasma Contamination in Cell Cultures: a Method for Reducing Fluorescence Photobleaching

Biotechnic & Histochemistry (1994)

Massimo Battaglia Deleana Pozzi Settimio Crimaldi Tiziana Parasassi

DNA fluorochrome staining with Hoechst 33258 bisbenzimide is commonly used for detection of mycoplasma contamination in cell cultures. Photobleaching of Hoechst 33258 is pronounced under the conditions of intense illumination, high magnification and resolution required for detection of mycoplasmas. To reduce photobleaching we investigated the effects of some antioxidant molecules, p-phenylenediamine (PPD), n-propyl gallate (NPG) and 1,4-diazabicyclo(2,2,2)octane (DABCO), which are known to reduce the fading rate of fluorescein. Mycoplasma-contaminated cell monolayers were stained with Hoechst 33258 and mounted in glycerol containing different amounts of antioxidant additives. The cells were examined in an epifluorescence microscope, and the emitted light intensity was recorded. Results showed that PPD and, to a lower degree, NPG, retarded the photobleaching of Hoechst 33258-stained cells, whereas DABCO was not effective. However, fluorescence half-life was increased about three-fold by NPG and almost 20-fold by PPD. The rate of fluorescence fading of Hoechst 33258 can therefore be retarded by PPD, with obvious advantages for reading and photographic recording of results.

Photobleaching

DABCO

Rhodamine

10.3109/10520299409106277

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Citations (34)

N-acetyl-l-cysteine fosters inactivation and transfer to endolysosomes of c-Src

Free Radical Biology and Medicine (2008)

Ewa K. Krasnowska Eugenia Pittaluga Anna Maria Brunati Roberto Brunelli Graziella Costa

10.1016/j.freeradbiomed.2008.09.012

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A Promise in the Treatment of Endometriosis: An Observational Cohort Study on Ovarian Endometrioma Reduction by N-Acetylcysteine

Evidence-based Complementary and Alternative Medicine (2013)

Maria Grazia Porpora Roberto Brunelli Graziella Costa Ludovica Imperiale Ewa K. Krasnowska

Urged by the unmet medical needs in endometriosis treatment, often with undesirable side effects, and encouraged by N-acetylcysteine (NAC) efficacy in an animal model of endometriosis and by the virtual absence of toxicity of this natural compound, we performed an observational cohort study on ovarian endometriosis. NAC treatment or no treatment was offered to 92 consecutive Italian women referred to our university hospital with ultrasound confirmed diagnosis of ovarian endometriosis and scheduled to undergo laparoscopy 3 months later. According to patients acceptance or refusal, NAC-treated and untreated groups finally comprised 73 and 72 endometriomas, respectively. After 3 months, within NAC-treated patients cyst mean diameter was slightly reduced (-1.5 mm) versus a significant increase (+6.6 mm) in untreated patients (P=0.001). Particularly, during NAC treatment, more cysts reduced and fewer cysts increased their size. Our results are better than those reported after hormonal treatments. Twenty-four NAC-treated patients—versus 1 within controls—cancelled scheduled laparoscopy due to cysts decrease/disappearance and/or relevant pain reduction (21 cases) or pregnancy (1 case). Eight pregnancies occurred in NAC-treated patients and 6 in untreated patients. We can conclude that NAC actually represents a simple effective treatment for endometriosis, without side effects, and a suitable approach for women desiring a pregnancy.

10.1155/2013/240702

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Citations (60)

In K562 and HL60 cells membrane ageing during cell growth is associated with changes in cholesterol concentration

Mechanisms of Ageing and Development (1997)

Moshe Levi Paul Wilson Shelly Nguyen Egidio Iorio O. Sapora

Laurdan

HL60

K562 cells

Cell membrane

Membrane Lipids

10.1016/s0047-6374(97)00047-x

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Citations (28)

Phototoxic effect of fluoroquinolones on two human cell lines

Toxicology in Vitro (2002)

Daniela Trisciuoglio Ewa K. Krasnowska A. Maggi Roberta Pozzi Tiziana Parasassi

Laurdan

Ethidium bromide

Phototoxicity

Cell membrane

HL60

Mechanism of Action

10.1016/s0887-2333(02)00029-2

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Citations (22)

Plastique: A synchrotron radiation beamline for time resolved fluorescence in the frequency domain

Review of Scientific Instruments (1991)

Gelsomina De Stasió N. Zema F. Antonangeli A. Savoia Tiziana Parasassi

PLASTIQUE is the only synchrotron radiation beamline in the world that performs time resolved fluorescence experiments in frequency domain. These experiments are extremely valuable sources of information on the structure and dynamics of molecules. We describe the beamline and some initial data.

10.1063/1.1142407

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Citations (3)

Alterations in Erythrocyte Membrane Lipids Induced by Low Doses of Ionizing Radiation as Revealed by 1,6-diphenyl-1,3,5-hexatriene Fluorescence Lifetime

International Journal of Radiation Biology (1991)

Tiziana Parasassi O. Sapora Anna Maria Giusti Gelsomina De Stasió Giampietro Ravagnan

Damage in membrane lipids induced by low doses of ionizing radiation in the presence of oxygen has been detected in rabbit erythrocyte ghosts labelled with 1,6-diphenyl-1,3,5-hexatriene (DPH). Multifrequency phase and modulation fluorometry was used to measure DPH fluorescence lifetime. This technique is particularly suited for the observation of heterogeneous fluorescence decays. DPH decay in erythrocyte membranes is described by a two-component continuous distribution of lifetimes. The value of the distribution width of the long-lived component is found to be affected by radiation-induced membrane lipid damage at doses as low as 0.5 Gy, well within the dose range used to measure cell survival. The width of the DPH lifetime distribution decreases when the ghosts are irradiated in the presence of oxygen. Such a decrease is a linear function of the logarithm of the dose. After a dose of 110 Gy and above, the fractional intensity of the short-lived component of the DPH decay increases linearly, indicating severe membrane damage. Experiments performed in the absence of oxygen do not show any change in the fluorescence parameters up to a dose of 550 Gy. The molecular identification of the produced damage has not been accomplished, but the necessity of oxygen to observe the damage suggests that hydroperoxides and lipids crosslinks are produced.

Radiosensitivity

Membrane Lipids

10.1080/09553009114550061

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Citations (47)

Intervillous circulation in intra-uterine growth restriction. Correlation to fetal well being

Placenta (2010)

Roberto Brunelli Gabriele Masselli Tiziana Parasassi Marco De Spirito Massimiliano Papi

Intervillous space

Intrauterine growth restriction

Placental Circulation

Fetal circulation

Placental insufficiency

Ductus venosus

10.1016/j.placenta.2010.09.004

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Citations (39)