Approximately 20–30% of endometrial carcinomas (EC) are characterized by mismatch repair (MMR) deficiency (dMMR) or microsatellite instability (MSI), and their testing has become part of the routine diagnosis. The aim of this study was to establish and compare the MMR status using various approaches. Immunohistochemistry (IHC), PCR-based MSI, and the detection of defects in the four key MMR genes (MLH1, PMS2, MSH2, and MSH6) via methylation-specific multiplex ligation-dependent probe amplification (MLPA) and targeted next-generation sequencing (NGS) were performed. MSH3 expression was also evaluated. A set of 126 early-stage EC samples were analyzed, 53.2% of which were dMMR and 46.8% of which were proficient MMR (pMMR) as determined using IHC, whereas 69.3% were classified as microsatellite stable, while 8.8% and 21.9% were classified MSI-low (MSI-L) and MSI-high (MSI-H), respectively. In total, 44.3% of the samples showed genetic or epigenetic alterations in one or more genes; MLH1 promoter methylation was the most common event. Although acceptable concordance was observed, there were overall discrepancies between the three testing approaches, mainly associated with the dMMR group. IHC had a better correlation with MMR genomic status than the MSI status determined using PCR. Further studies are needed to establish solid conclusions regarding the best MMR assessment technique for EC.

10.3390/ijms241914468

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Citations (9)

Breast Conserving Surgery Guided with Magnetic Seeds vs Wires: la Paz University Hospital Experience

Research Square (Research Square) (2022)

E Moreno-Palacios Covadonga Martí Laura Frías Marcos Meléndez Adolfo Loayza

Abstract Background : In recent years, there has been an increase in breast surgery for nonpalpable lesions. Traditionally, wires have been used to guide the resection of nonpalpable breast tumors. Recently, several devices have been commercialized as alternatives to classical wires, such as magnetic seeds (Magseed®). The aim of this study is to describe our initial experience using magnetic seeds (Magseed®) to guide breast-conserving surgery in nonpalpable breast lesions and compare the use of magnetic seed with wires to guide breast-conserving surgery, in terms of clinical and pathological characteristics. Methods : We performed a retrospective study including all breast-conserving surgeries for nonpalpable breast lesions <16 mm, from June 2018 to May 2021. We compared breast-conserving surgeries guided with magnetic seeds to those guided with wires, analyzing: tumor, and patient characteristics, surgical time, and pathological results of the surgical specimens. Results : Data from 225 cases was collected, 149 magnetic seeds and 76 wires. The breast lesion was localized in every case. Both cohorts were similar regarding clinical and pathological characteristics. We found significant statistical differences (p <0.02) in terms of the median volume (cm3) of the excised specimen, which was lower (29.3%) in the magnetic seed group compared with the wire group (32.5 [20.5-60.0] / 46.0 [20.3-118.7]). We did not find significant differences regarding surgical time (min), and affected or close margins. Conclusion : In our experience, the use of magnetic seed (Magseed®) is a feasible option to guide breast-conserving surgery of non-palpable lesions and enable us to resect less breast tissue.

Breast-conserving surgery

Breast tumor

Breast tissue

10.21203/rs.3.rs-2233662/v1

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Release of Contracting Autacoids by Aortae of Normal and Atherosclerotic Rabbits

Journal of Cardiovascular Pharmacology (1992)

Paola Patrignani L. Daffonchio Alicia Hernández Raffaele De Caterina Gualtiero Pelosi

Summary: The aim of our study was to examine the release of various lipid and peptide contracting autacoids by aortae of normal and atherosclerotic rabbits. Leukotriene (LT) E4, an enzymatic derivative of LTC4, thromboxane (Tx) B2, and endothelin-1 (ET-1) were measured by radioimmunoassay techniques in aortic preparations of normal and cholesterol-fed rabbits. Intact aortae of normal rabbits incubated with the calcium ionophore A23187 for 1 h at 37°C released LTE4 and TxB2 (22 ± 3.5 and 14.8 ± 2 pg/mg of tissue, respectively, mean ± SEM, n = 33). Removal of aortic endothelium was associated with a significant reduction in LTE4 (44%) and TxB2 (58%) release. In aortic preparations from cholesterol-fed rabbits, the release of LTE4 was significantly enhanced (41 ± 8 pg/mg of tissue, mean ± SEM, n = 27) whereas TxB2 was not significantly altered. No detectable amounts of ET-1 were measured after 1 h of incubation. However, at 4 h, an endothelium-dependent release of ET-1 from normal aortae was demonstrated. In atherosclerotic aortae, ET-1 release was significantly higher than in controls (10 ± 1.3 vs. 5 ± 0.5 pg/cm2, mean ± SEM, n = 16). We conclude that enhanced formation of vasoconstrictor autacoids may contribute to altered vasomotion of atherosclerotic blood vessels.

Autacoid

10.1097/00005344-199204002-00059

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Citations (19)

A multivariate analysis of the prognostic impact of tumor burden, surgical timing and complexity after complete cytoreduction for advanced ovarian cancer

Gynecologic Oncology (2020)

Martina Aida Ángeles Agnieszka Rychlik Bastien Cabarrou Emanuela Spagnolo Frédéric Guyon

Debulking

10.1016/j.ygyno.2020.06.495

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Citations (15)

Nodal involvement evaluation in advanced cervical cancer: a single institutional experience.

PubMed (2013)

Cristina González-Benítez Ignacio Zapardiel P. Salas María Dolores Diestro Alicia Hernández

To assess the usefulness of different imaging techniques in the detection of nodal involvement in patients with advanced cervical carcinoma. Moreover, to analyze the correlation between the presurgical (FIGO) and postsurgical (pTNM) staging classifications.All patients diagnosed with advanced cervical cancer (FIGO Stages IIB-IV) from 2005 to 2012 were selected. The medical charts of 51 patients that underwent presurgical assessment with posterior surgical staging by means of para-aortic lymphadenectomy, were reviewed. Nodal status assessment by computed tomography scan (CT scan), magnetic resonance imaging (MRI), positron emission tomography (PET), and sonography was compared, as well as the size given in imaging techniques compared to the final pathologic report information.Presurgical analysis by CT scan, MRI, PET, and sonography showed pelvic nodal involvement in 51.3% of patients, and para-aortic involvement in 30.8% of cases. CT scan showed positive pelvic nodes in 35% of cases, but pathologic confirmation was observed in just 17.6% of cases. However, MRI resulted in higher rates of up to 48.8% of cases. Concerning para-aortic nodal involvement, CT scan showed positive nodes in 25% of cases, MRI in 3.2% of cases, and the pathologic report in 15.6% of cases. The authors found significant differences between staging groups among both classifications (FIGO vs. pTNM; p < 0.001). Eight cases (15.7%) were understaged by FIGO classification.Despite all imaging techniques available, none has demonstrated to be efficient enough to avoid the systematic study of para-aortic nodal status by means of surgical evaluation.

Lymphadenectomy

Source

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Role of single-site and mini-laparoscopy in gynecologic surgery

Minerva Obstetrics and Gynecology (2021)

Elsa Delgado-Sánchez John A. PEAY-PINACHO Alicia Hernández Julio Álvarez Bernardi Ignacio Zapardiel

INTRODUCTION: Laparoscopy is a surgical procedure that has been used widely in medicine over the last thirty years. In gynecology, laparoscopy is the "gold standard" for the majority of gynecological procedures, as its superiority over laparotomy has been widely demonstrated. In recent years, the current trend of gynecologists is to make laparoscopy surgery even less invasive by reducing the number of incisions in the skin, as it happens with laparoendoscopic single-site surgery, or by reducing the size of them as in mini-laparoscopy. The aim of this work was to perform an extensive review and update of the evolution of single-port surgery and mini-laparoscopic surgery in gynecology as well as to evaluate its current role in this field.EVIDENCE ACQUISITION: A systematic review was performed during April and May 2020. PRISMA guidelines were followed for the literature search.EVIDENCE SYNTHESIS: The main objective of performing less invasive procedures is to reduce both intraoperative complications (decreased risk of bleeding or damage to internal organs), and postoperative ones (hernias through the trocar) and improve cosmetic results. Results of studies about LESS and mini-LPS showed encouraging results, being both of them safe with a similar perioperative and postoperative outcome. They have the approval of the international surgical community as well as patients' satisfaction with cosmetic results.CONCLUSIONS: Minimally invasive surgery is the present and future in gynecological surgery. More prospective randomized trials are needed in order to obtain valid results and affirm that both LESS and Mini-LPS are superior to conventional laparoscopy.

Gold standard (test)

Gynecological surgery

Invasive surgery

10.23736/s2724-606x.20.04607-9

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Citations (8)

Interleukin-1β: A possible mediator of lung inflammation and airway hyperreactivity

Pharmacological Research (1991)

Alicia Hernández C. Omini L. Daffonchio

Proinflammatory cytokine

10.1016/1043-6618(91)90043-w

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