Guiquan Ding

Qingdao University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Yichen Yin

Qingdao University

Wei Wang

Northeast Petroleum University

Hui Wang

Zhejiang Lab

Xiaomei Zhang

Peking University International Hospital

Pengyi Hou

Ningxia Medical University

Xiaowen Tang

Soochow University

Shiwei Sun

Qingdao University

Hongwei Zhang

Huazhong University of Science and Technology

Cooperative Institutions

Qingdao University

Peking University

First Affiliated Hospital of Anhui Medical University

Anhui Medical University

Peking University International Hospital

Fudan University

Yuhuangding Hospital

Northeast Petroleum University

Jilin Agricultural University

National Engineering Research Center for Wheat

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Identification of coniferyl ferulate as the bioactive compound behind the xanthine oxidase inhibitory activity of Chuanxiong Rhizome

Journal of Functional Foods (2022)

Hui Wang Hongwei Zhang Xiaomei Zhang Yichen Yin Guiquan Ding

Xanthine oxidase (XO) has been well-recognized as a key enzyme in the pathogenesis of hyperuricemia. In this study, Chuanxiong Rhizome, a tonic food widely consumed in China, was found to inhibit XO activity. The potential XO inhibitory ingredients were screened by UPLC-ZenoTOF system and bioactivity assays. Coniferyl ferulate (CF), a main constitute of Chuanxiong Rhizome, was identified as a potent XO inhibitor with an IC50 value (1.97 ± 0.11 μM) lower than that of allopurinol (2.49 ± 0.07 μM). Enzyme-kinetic assays showed that CF reversibly inhibited XO in an uncompetitive manner. And hydrogen bonds played crucial roles on the binding interaction according to the fluorescence titration and molecular docking. Moreover, the combination of CF with allopurinol exhibited a synergistic inhibitory effect on XO. Our results revealed for the first time the XO inhibitory effect of CF from Chuanxiong Rhizome, which shed light on its potential application for developing anti-hyperuricemia agents.

Allopurinol

IC50

Uncompetitive inhibitor

10.1016/j.jff.2022.105378

Cite

Citations (12)