Javier Regidor‐Cerrillo

Universidad Complutense de Madrid

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Luís Miguel Ortega-Mora

Universidad Complutense de Madrid

119

Júlio Benavides

Universidad de León

Esther Collantes-Fernández

Universidad Complutense de Madrid

Pilar Horcajo

Universidad Complutense de Madrid

GÁ

Gema Álvarez‐García

Universidad Complutense de Madrid

Ignacio Ferre

Universidad Complutense de Madrid

Valentín Pérez Pérez

Instituto de Ganadería de Montaña

Mercedes Gómez-Bautista

Universidad Complutense de Madrid

Marta García-Sánchez

Universidad Complutense de Madrid

Laura Jiménez-Pelayo

Universidad Complutense de Madrid

Cooperative Institutions

Universidad Complutense de Madrid

Consejo Superior de Investigaciones Científicas

National Agricultural Technology Institute

Consejo Nacional de Investigaciones Científicas y Técnicas

Universidad de León

Cambridge University Press

Instituto de Salud Carlos III

Universidad de Zaragoza

Instituto Nacional de Tecnologia

University of Bern

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Abortos de fase aguda en la toxoplasmosis ovina. Caracterización lesional y posible patogenia

Pablo Castaño M. Fuertes Ignacio Ferre M. Fernández Fernández Ma del Carmen Ferreras

6 paginas, 1 tabla.--Trabajo presentado a las XXXIX Congreso Nacional y XV Internacional de la Sociedad Espanola de Ovinotecnia y Caprinotecnia (SEOC), (Ourense, 17-19 de septiembre de 2014).

Source

Cite

Citations (0)

Diagnóstico molecular de Neospora caninum en fetos abortados espontáneamente en bovinos de Uruguay.

Veterinaria (Montevideo) (2021)

Javier Regidor‐Cerrillo Cristina Easton Florencia Pieruccioni Agustín Romer Pablo Peraza

ResumenEn Uruguay el diagnóstico de rutina de Neospora caninum se realiza fundamentalmente por estudios histopatológicos de los fetos abortados, complementando esta información con resultados de serología y/o Inmunohistoquímica (IHQ).El objetivo de este estudio fue estandarizar un protocolo de amplificación de secuencias mediante PCR (polymerase chain reaction) para la detección de Neospora caninum en órganos de fetos abortados espontáneamente en bovinos para su incorporación a la rutina diagnóstica.Con este propósito se realizó un estudio retrospectivo utilizando muestras congeladas de archivo, de un periodo comprendido entre los años 2013 y 2016, con diagnóstico positivo o negativo a Neospora caninum.Los resultados mostraron que de 31 muestras positivas a Neospora caninum por las técnicas de rutina, 26 fueron positivas por PCR (84%), demostrando una alta sensibilidad de diagnóstico de esta técnica.Todas las muestras con diagnóstico negativo mediante histopatología (n=20) fueron confirmadas como negativo mediante PCR.La comparación de los resultados de PCR con los de histopatología, mediante el test de Cohen's Kappa, señalaron un acuerdo muy alto (81%, p<0.001) entre ambos diagnósticos.Dichos resultados sumados a la simplicidad y rapidez de la técnica podrían sugerir a la PCR como alternativo de la IHQ como técnica confirmatoria en el diagnóstico de rutina.También permitiría realizar análisis retrospectivos de muestras archivadas, paso fundamental para futuros estudios genéticos de la población de Neospora caninum presente en Uruguay.

10.29155/vet.57.216.3

Cite

Citations (0)

Neospora caninum infection induces an isolate virulence-dependent pro-inflammatory gene expression profile in bovine monocyte-derived macrophages

Parasites & Vectors (2020)

Marta García-Sánchez Laura Jiménez-Pelayo Pilar Horcajo Esther Collantes-Fernández Luís Miguel Ortega-Mora

Abstract Background Neospora caninum is an obligate intracellular parasite, and its ability to survive inside host immune cells may be a key mechanism for the establishment of infection in cattle. In vitro studies carried out by our group have shown that N. caninum is able to replicate in bovine macrophages (MØs), alter their microbicidal mechanisms and exploit their motility. Furthermore, host-cell control seems to be isolate virulence-dependent. Methods To investigate the molecular basis underlying the innate responses in MØs against N. caninum and the mechanisms of parasite manipulation of the host cell environment, the transcriptome profile of bovine monocyte-derived MØs infected with high-virulence (Nc-Spain7) or low-virulence (Nc-Spain1H) N. caninum isolates was studied. Results Functional enrichment revealed upregulation of genes involved in chemokine signalling, inflammation, cell survival, and inhibition of genes related with metabolism and phagolysosome formation. MØs activation was characterized by the induction of a predominantly M1 phenotype with expression of TLR2 , TLR3 and TLR9 and activation of the NF-ƙB signalling pathway. Heat-killed N. caninum tachyzoites failed to activate NF-ƙB, and to inhibit lysosomal activity and apoptosis, which indicates active modulation by the parasite. The FoxO signalling pathway, Th1-Th2 differentiation, glycosaminoglycan degradation and apoptosis were pathways enriched only for low virulent Nc-Spain1H infection. In addition, Nc-Spain1H infection upregulated the IL12A and IL8 pro-inflammatory cytokines, whereas IL23 was downregulated by high virulent Nc-Spain7. Conclusions This study revealed mechanisms implicated in the recognition of N. caninum by bovine MØs and in the development of the subsequent immune response. NF-ƙB seems to be the main signalling pathway implicated in the pro-inflammatory bovine MØs response against this pathogen. Apoptosis and phagolysosome maturation are processes repressed by N. caninum infection, which may guarantee its intracellular survival. The results also indicate that Nc-Spain7 may be able to partially circumvent the pro-inflammatory response whereas Nc-Spain1H induces a protective response to infection, which may explain the more efficient transmission of the high-virulence Nc-Spain7 isolate observed in vivo .

CCL22

10.1186/s13071-020-04239-3

Cite

Citations (14)

Microsatellite markers for the molecular characterization of Neospora caninum: Application to clinical samples

Veterinary Parasitology (2009)

Susana Pedraza‐Díaz Virginia Marugán‐Hernández Esther Collantes-Fernández Javier Regidor‐Cerrillo Silvia Rojo-Montejo

10.1016/j.vetpar.2009.07.043

Cite

Citations (44)

Additional file 4 of Neospora caninum infection induces an isolate virulence-dependent pro-inflammatory gene expression profile in bovine monocyte-derived macrophages

Figshare (2020)

Marta García-Sánchez Laura Jiménez-Pelayo Pilar Horcajo Esther Collantes-Fernández Luís Miguel Ortega-Mora

Additional file 4: Table S4.Bos taurus differentially expressed genes in bovine macrophages infected with Neospora caninum Nc-Spain1H isolate (MØ1H) versus non-infected macrophages (MØC). Table S5.Bos taurus differentially expressed genes in bovine macrophages infected with Neospora caninum Nc-Spain7 isolate (MØ7) versus non-infected macrophages (MØC). Table S6.Bos taurus differentially expressed genes in bovine macrophages inoculated with Neospora caninum heat-killed tachyzoites (MØHK) versus non-infected macrophages (MØC). Table S7.Bos taurus differentially expressed genes in bovine macrophages infected with Neospora caninum Nc-Spain7 isolate (MØ7) versus those infected with Nc-Spain1H isolate (MØ1H).

Monocyte

10.6084/m9.figshare.12720091

Cite

Citations (0)

PAR volume 140 issue 5 Cover and Back matter

Parasitology (2013)

Xiaohong Huang Yang Xu Guowei Guo Qianqian Lin Zhongfeng Ye

An abstract is not available for this content so a preview has been provided. As you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Content (measure theory)

10.1017/s0031182013000280

Cite

Citations (0)

Identificación de proteínas Neospora caninum implicadas en procesos de invasión y virulencia

Proteómica: revista de la Sociedad Española de Proteómica (2010)

Virginia Marugán‐Hernández Javier Regidor‐Cerrillo Gema Álvarez‐García Fiona M. Tomley Adriana Aguado-Martínez

Source

Cite

Citations (0)

Toxoplasma gondii experimental infection in pregnant sheep at early, mid and late gestation. Pathological response and parasite distribution

Pablo Castaño M. Fuertes Javier Regidor‐Cerrillo Camino González Lanza Javier Moreno Gonzalo

Trabajo presentado al: ApiCOWplexa 2015 3rd lnternational Meeting on Apicomplexan Parasites in Farm Animals. (Edinburgh, Escocia, Reino Unido, 30 junio-3 julio 2015).

Toxoplasmosis

Source

Cite

Citations (0)

Neospora caninum infection in breeder bulls: seroprevalence and comparison of serological methods used for diagnosis

Veterinary Parasitology (2004)

Andrea Caetano-da-Silva Ignacio Ferre Gorka Adúriz Gema Álvarez‐García I. del-Pozo

Seroprevalence

Neospora

10.1016/j.vetpar.2004.06.023

Cite

Citations (19)

Experimental ovine toxoplasmosis: influence of the gestational stage on the clinical course, lesion development and parasite distribution

Veterinary Research (2016)

Pablo Castaño M. Fuertes Javier Regidor‐Cerrillo Ignacio Ferre Miguel Fernández

The relation between gestational age and foetal death risk in ovine toxoplasmosis is already known, but the mechanisms involved are not yet clear. In order to study how the stage of gestation influences these mechanisms, pregnant sheep of the same age and genetic background were orally dosed with 50 oocysts of Toxoplasma gondii (M4 isolate) at days 40 (G1), 90 (G2) and 120 (G3) of gestation. In each group, four animals were culled on the second, third and fourth week post infection (pi) in order to evaluate parasite load and distribution, and lesions in target organs. Ewes from G1 showed a longer period of hyperthermia than the other groups. Abortions occurred in all groups. While in G2 they were more frequent during the acute phase of the disease, in G3 they mainly occurred after day 20 pi. After challenge, parasite and lesions in the placentas and foetuses were detected from day 19 pi in G3 while in G2 or G1 they were only detected at day 26 pi. However, after initial detection at day 19 pi, parasite burden, measured through RT-PCR, in placenta or foetus of G3 did not increase significantly and, at in the third week pi it was lower than that measured in foetal liver or placenta from G1 to G3 respectively. These results show that the period of gestation clearly influences the parasite multiplication and development of lesions in the placenta and foetus and, as a consequence, the clinical course in ovine toxoplasmosis.

Toxoplasmosis

10.1186/s13567-016-0327-z

Cite

Citations (35)