A novel approach of direct end labelling of telomeres is presented. Chromosome-sized, agarose-embedded DNA was treated with T4 DNA polymerase to remove protruding 3' end of telomeres and to generate single-stranded 5' ends. The DNA was then labelled by the same enzyme in the presence of [alpha-32P]dGTP and cold dATP and dTTP. Labelled yeast chromosomes separated by pulsed field gel electrophoresis maintained their integrity. Digestion of yeast chromosomes separated in pulsed field gels with a restriction nuclease (HinfI), followed by conventional electrophoresis in the second dimension, resulted in a fingerprint-like pattern of labelled telomeres. This was very similar to the hybridization pattern of a similar two-dimensional gel probed with cloned yeast telomeric sequence. The same approach enabled us to label telomeres in soybean, determine their size, and to reveal polymorphisms in the length of telomeres between the closely related subspecies Glycine max (soybean) and Glycine soja.

10.1139/g93-031

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Citations (5)

Map order and linkage distances of molecular markers close to the supernodulation (nts-1) locus of soybean

MGG Molecular & General Genetics (1997)

Alexander Kolchinsky D. Landau‐Ellis Peter M. Gresshoff

Molecular marker

Bulked segregant analysis

Genetic linkage

Glycine soja

10.1007/s004380050387

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Citations (28)

"Portraying" of plant genomes using polymerase chain reaction amplification of ribosomal 5S genes

Genome (1991)

Alexander Kolchinsky M D Kolesnikova Evgeny Ananiev

Nontranscribed spacers of plant genes coding for ribosomal 5S RNA were amplified using the polymerase chain reaction. Primers were synthesized that were complementary to 3′ (direct) and 5′ (reverse) ends of the coding region and that are universal for higher plants. The patterns of polymerase chain reaction products are species and, sometimes, variety specific. The use of this approach for identification of barley 5S genes in chromosome-addition lines of wheat is discussed. This principle can be applied for the "portraying" of other tandem repetitive genes containing divergent regions.Key words: polymerase chain reaction, 5S genes, plants, DNA polymorphism.

10.1139/g91-158

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Citations (31)

Organization of Lac Repressor, RNA Polymerase and Histones of DNA

Springer eBooks (1979)

A.D. Mirzabekov R. Beabealashvilli Alexander Kolchinsky A. F. Melnikova V. Schick

Histone-modifying enzymes

Transcription

10.1007/978-1-4684-3465-1_4

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Conditionally tumorigenic clone of C6 cells is induced by gamma-irradiation.

PubMed (1998)

Alexander Kolchinsky

Established cell lines are often heterogeneous in their response to ionizing radiation. The tumorigenicity of some cells can be rapidly induced by gamma-radiation.The tumorigenicity of individual clones of C6 rat glioblastoma cells and the effect of gamma-radiation on their growth in vivo was studied by intracranial implantation into Wistar rats.A clone was found, C6#11, that was conditionally tumorigenic. It either did not form a tumor, or grew into a small encapsulated tumor, and did not cause death or neurological deficits in the animals. Gamma-irradiation of the animals (head only) with implanted C6#11 induced a fully developed invasive tumor that killed them within 25 days. Three grays of radiation of C6#11 cells two hours prior to implantation induced their tumorigenicity as well. The in vitro characteristics of the C6#11 cells as compared to a highly tumorigenic clone did not correlate with their behavior in vivo.The C6#11 clone is the first model of an experimental tumor that acquires tumorigenicity in an immuno-competent host immediately after low dose irradiation of the cells.

clone (Java method)

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Molecular and Genetic Insights into Shoot Control of Nodulation in Soybean

Springer eBooks (1997)

Peter M. Gresshoff Keishi Senoo Jaime Padilla Debbie Landau-Ellis Anatoli Filatov

Lateral root

10.1007/978-3-642-59112-9_56

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