Wei Shen

Qingdao Agricultural University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Yong Zhao

Chinese Academy of Agricultural Sciences

Wei Ge

Qingdao Agricultural University

Shun‐Feng Cheng

Qingdao Agricultural University

Junjie Wang

Qingdao Agricultural University

Xiao‐Feng Sun

Qingdao Agricultural University

Qing‐Yuan Sun

Guangdong Provincial People's Hospital

Massimo De Felici

University of Rome Tor Vergata

Xifeng Zhang

Qingdao Agricultural University

Shen Yin

Qingdao Agricultural University

Fa‐Li Zhang

Qingdao Agricultural University

Cooperative Institutions

Qingdao Agricultural University

197

Chinese Academy of Sciences

Peking University

Chinese Academy of Medical Sciences & Peking Union Medical College

Sun Yat-sen University

Shanghai Jiao Tong University

Chinese Academy of Agricultural Sciences

Northwest A&F University

Sichuan University

University of Chinese Academy of Sciences

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Comparative study on effect of different promoters on expression of cry1Ac in Bacillus thuringiensis chromosome

Journal of Applied Microbiology (2007)

Chaoyin Yue Wei Shen Ming Sun Lin Li Faju Chen

The aim of this work was to investigate the effect of cry3A promoter on the expression of cry1Ac in Bacillus thuringiensis chromosome and stably enhance the production of different cry genes under the control of cry3A promoter.The cry1Ac gene, which is specific to Lepidopteran larvae, was integrated into the chromosome of a B. thuringiensis plasmid-free and acrystalliferous strain BMB171, under the control of cry3A promoter and cry1Ac promoter, respectively. The expression of cry1Ac genes in the chromosome of host strain was investigated. The results from sodium dodecyl sulfate-polyacrymide gel electrophoresis, crystal observation and bioassay showed that either integrated with cry3A promoter (cry3Apro-cry1Ac) or with its native promoter (cry1Acpro-cry1Ac), cry1Ac gene could efficiently and stably express in the chromosome. The production of cry3Apro-cry1Ac gene was higher than that of cry1Acpro-cry1Ac gene.The cry3A promoter enhanced the expression of cry1Ac gene efficiently either on the chromosome or on the plasmid in B. thuringiensis strain.So far, the comparative studies on cry3A promoter and other cry promoters were carried on B. thuringiensis plasmids. This system offers an additional method for potentially improving the efficacy of B. thuringiensis insecticidal proteins efficiently, stably and safely.

Cry1Ac

Bacillus thuringiensis

10.1111/j.1365-2672.2006.03269.x

Cite

Citations (10)

Hydrogen Sulfide and/or Ammonia Reduces Spermatozoa Motility through AMPK/AKT Related Pathways

Scientific Reports (2016)

Yong Zhao Weidong Zhang Xinqi Liu Pengfei Zhang Yanan Hao

Abstract A number of emerging studies suggest that air pollutants such as hydrogen sulfide (H 2 S) and ammonia (NH 3 ) may cause a decline in spermatozoa motility. The impact and underlying mechanisms are currently unknown. Boar spermatozoa ( in vitro ) and peripubertal male mice ( in vivo ) were exposed to H 2 S and/or NH 3 to evaluate the impact on spermatozoa motility. Na 2 S and/or NH 4 Cl reduced the motility of boar spermatozoa in vitro. Na 2 S and/or NH 4 Cl disrupted multiple signaling pathways including decreasing Na + /K + ATPase activity and protein kinase B (AKT) levels, activating Adenosine 5′-monophosphate (AMP)-activated protein kinase (AMPK) and phosphatase and tensin homolog deleted on chromosome ten (PTEN), and increasing reactive oxygen species (ROS) to diminish boar spermatozoa motility. The increase in ROS might have activated PTEN, which in turn diminished AKT activation. The ATP deficiency (indicated by reduction in Na + /K + ATPase activity), transforming growth factor (TGF β ) activated kinase-1 (TAK1) activation, and AKT deactivation stimulated AMPK, which caused a decline in boar spermatozoa motility. Simultaneously, the deactivation of AKT might play some role in the reduction of boar spermatozoa motility. Furthermore, Na 2 S and/or NH 4 Cl declined the motility of mouse spermatozoa without affecting mouse body weight gain in vivo. Findings of the present study suggest that H 2 S and/or NH 3 are adversely associated with spermatozoa motility.

10.1038/srep37884

Cite

Citations (56)

Cyanidin-3-O-glucoside Mitigates the Ovarian Defect Induced by Zearalenone via p53-GADD45a Signaling during Primordial Follicle Assembly

Journal of Agricultural and Food Chemistry (2023)

Jingya Wang Fa‐Li Zhang Xiu‐Xiu Li Ke‐Xin Zhu Ning Zuo

Zearalenone (ZEN) is well known as a kind of endocrine disruptor whose exposure is capable of causing reproductive toxicity in animals. Cyanidin-3-O-glucoside (C3G) is a derivative of cyanidin and owns multiple biofunctions, and prior efforts have suggested that C3G has therapeutic actions for reproductive diseases. In this article, a ZEN exposure model during primordial follicle assembly was constructed using the in vitro culture platform of neonatal mouse ovaries. We investigated the protective effect of C3G on ZEN-induced ovarian toxicity during primordial follicle assembly in mice, as well as its potential mechanism. Interestingly, we observed that C3G could effectively protect the ovary from ZEN damage, mainly by restoring primordial follicle assembly, which upregulated the expression of LHX8 and SOHLH1 proteins and relieved ZEN-induced DNA damage. Next, to explore the mechanism by which C3G rescued ZEN-induced injury, we performed RNA sequencing (RNA-seq). The bioinformatic analysis illustrated that the rescue pathway of C3G was associated with p53-Gadd45a signaling and cell cycle. Then, western blotting and flow cytometry results revealed that C3G restored the expression levels of cyclin-dependent kinase 6 (CDK6) and cyclin D2 (CCND2) and regulated the ovarian cell cycle to normal. In conclusion, our findings manifested that C3G could alleviate ZEN-induced primordial follicle assembly impairment by restoring the cell cycle involved in p53-GADD45a signaling.

Cyclin B1

10.1021/acs.jafc.3c03315

Cite

Citations (2)

The root of reduced fertility in aged women and possible therapentic options: Current status and future perspects

Molecular Aspects of Medicine (2013)

Jie Qiao Zhen‐Bo Wang Huai-Liang Feng Yi‐Liang Miao Qiang Wang

Ovarian Reserve

Unexplained infertility

10.1016/j.mam.2013.06.001

Cite

Citations (137)

Epigallocatechin gallate improves the quality of diabetic oocytes

Biomedicine & Pharmacotherapy (2023)

Shuo Chao Lijun Li Jun Lu Shu‐Xian Zhao Minghui Zhao

Maternal diabetes compromises the quality and developmental potential of oocytes. Therefore, it is important to study how to ameliorate the adverse effects of diabetes on oocyte quality. Epigallocatechin gallate (EGCG) has a variety of physiological activities, including anti-inflammatory, antioxidant, and anti-diabetes. In the present study, we evaluated the effect of EGCG on the maturation of diabetic oocytes in vitro.

SOD2

MFN2

10.1016/j.biopha.2023.114267

Cite

Citations (6)

Melatonin improves meiosis maturation against diazinon exposure in mouse oocytes

Life Sciences (2022)

Yang Gai Manyu Zhang Peng-Yuan Ji You Rongjing Zhao‐Jia Ge

In vitro maturation

10.1016/j.lfs.2022.120611

Cite

Citations (8)

Additional file 3 of Gut microbiota-testis axis: FMT improves systemic and testicular micro-environment to increase semen quality in type 1 diabetes

Figshare (2022)

Yanan Hao Yanni Feng Xiaowei Yan Liang Chen Ruqing Zhong

Additional file 3: Data Set 1. Blood metabolites raw data.

Semen quality

10.6084/m9.figshare.19656894

Cite

Citations (0)

DNA Double-Strand Breaks Induce the Nuclear Actin Filaments Formation in Cumulus-Enclosed Oocytes but Not in Denuded Oocytes

PLoS ONE (2017)

Ming‐Hong Sun Mo Yang Feng-Yun Xie Wei Wang Lili Zhang

As a gamete, oocyte needs to maintain its genomic integrity and passes this haploid genome to the next generation. However, fully-grown mouse oocyte cannot respond to DNA double-strand breaks (DSBs) effectively and it is also unable to repair them before the meiosis resumption. To compensate for this disadvantage and control the DNA repair events, oocyte needs the cooperation with its surrounding cumulus cells. Recently, evidences have shown that nuclear actin filament formation plays roles in cellular DNA DSB repair. To explore whether these nuclear actin filaments are formed in the DNA-damaged oocytes, here, we labeled the filament actins in denuded oocytes (DOs) and cumulus-enclosed oocytes (CEOs). We observed that the nuclear actin filaments were formed only in the DNA-damaged CEOs, but not in DOs. Formation of actin filaments in the nucleus was an event downstream to the DNA damage response. Our data also showed that the removal of cumulus cells led to a reduction in the nuclear actin filaments in oocytes. Knocking down of the Adcy1 gene in cumulus cells did not affect the formation of nuclear actin filaments in oocytes. Notably, we also observed that the nuclear actin filaments in CEOs could be induced by inhibition of gap junctions. From our results, it was confirmed that DNA DSBs induce the nuclear actin filament formation in oocyte and which is controlled by the cumulus cells.

MDia1

10.1371/journal.pone.0170308

Cite

Citations (20)

Toxic effects and possible mechanisms following malathion exposure in porcine granulosa cells

Environmental Toxicology and Pharmacology (2018)

Wei Wang Leilei Yang Shi‐Ming Luo Jun‐Yu Ma Yong Zhao

Toxicant

Granulosa cell

10.1016/j.etap.2018.11.002

Cite

Citations (23)

Cytotoxicity and DNA Damage Caused from Diazinon Exposure by Inhibiting the PI3K-AKT Pathway in Porcine Ovarian Granulosa Cells

Journal of Agricultural and Food Chemistry (2018)

Wei Wang Shi‐Ming Luo Jun‐Yu Ma Wei Shen Shen Yin

Organophosphorus insecticide diazinon (DZN) is diffusely used in agriculture, home gardening, and crop peats. Much work so far has focused on the link between DZN exposure and the occurrence of neurological diseases, while little is known on the reproductive toxicological assessment on DZN exposure. This research aimed to investigate the underlying mechanisms of toxic hazards for DZN exposure on cultured porcine ovarian granulosa cells. We analyzed the oxidative stress, energy metabolism, DNA damage, apoptosis, and autophagy by using high-throughput RNA-seq, immunofluorescence, Western blotting, and real-time PCR. The combined data demonstrated that DZN exposure could cause excessive ROS and DNA damage, which induced apoptosis and autophagy by inhibiting the PI3K-AKT pathway. The down-regulated CYP19A1 protein and granulosa cell deaths increase the risk for developing premature ovarian failure and follicular atresia. In conclusion, DZN exposure has obvious reproductive toxicity by induction of granulosa cell death through pathways connected to DNA damage and oxidative stress by inhibiting the PI3K-AKT pathway.

Follicular atresia

10.1021/acs.jafc.8b05194

Cite

Citations (53)