Alexander I. Shevchenko

Institute of Cytology and Genetics

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Suren M. Zakian

Institute of Cytology and Genetics

ИС

И. С. Захарова

Institute of Cytology and Genetics

N. A. Mazurok

Institute of Cytology and Genetics

S. P. Medvedev

Institute of Cytology and Genetics

Eugeny A. Elisaphenko

Institute of Cytology and Genetics

ЭВ

Э. В. Григорьева

Institute of Chemical Biology and Fundamental Medicine

Elena Vladimirovna Dementyeva

Institute of Cytology and Genetics

Tatyana B. Nesterova

University of Oxford

Neil Brockdorff

University of Oxford

M. K. Zhiven

Institute of Cytology and Genetics

Cooperative Institutions

Institute of Cytology and Genetics

Russian Academy of Sciences

Siberian Branch of the Russian Academy of Sciences

Ministry of Health of the Russian Federation

Novosibirsk State University

Institute of Chemical Biology and Fundamental Medicine

Meshalkin National Medical Research Center

Novosibirsk Scientific Research Institute of Traumatology and Orthopedics. Ya.L. Tsivyan

State Research Center of the Russian Federation

Centre National de la Recherche Scientifique

Author Statistics

Papers

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Stem Cells Giving Rise to Extraembryonic Tissues

Bulletin of Experimental Biology and Medicine (2011)

Э. В. Григорьева Alexander I. Shevchenko Antonina I. Zhelezova Alexander G. Shilov N. A. Mazurok

Trophoblast

10.1007/s10517-011-1179-1

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Citations (2)

Stratification of risks of tuberculosis relapses during the pandemic of the novel coronavirus infection

Tuberculosis and lung diseases (2023)

С. В. Смердин N. N. Sheshelyakina Alexander I. Shevchenko М. А. Плеханова R. I. Ludanny

The objective : to assess risk factors for tuberculosis relapse during the COVID-19 pandemic. Results. During the retrospective study, medical documents of patients treated for tuberculosis in 2020-2022 were analyzed. 140 patients above 18 years old with confirmed tuberculosis were included: newly diagnosed tuberculosis – 50 patients, early relapses – 50 patients, and late relapses – 40 patients. At the first stage, significant factors for relapse development were identified (ANOVA): diabetes (F=19402,8; p=0,000000), substances abuse (F=547,6; p=0,000000), alcohol abuse (F=149,7; p=0,000000), MDR MTB (F=107,8; p=0,000000), HIV infection (F=72,4; p=0,000000), imprisonment (F=49,5; p=0,000000), chronic respiratory diseases (F=47,1; p=0,000000), smoking (F=29,0; p=0,000000), and social status (F=28,9; p= 0,000000). At the second stage (cluster analysis), factors implementing the risks of development of early or late relapses were identified. As well as before the COVID-19 pandemic, social, medical and biological risk factors play a significant role in the development of tuberculosis relapses; history of COVID-19 was not one of the risk factors; and stratification of the risk factors made it possible to identify factors that contribute to development of both early and late relapses and to determine preventive measures.

Pandemic

Alcohol abuse

10.58838/2075-1230-2023-101-2-87-93

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Citations (2)

Monoallelic gene expression in mammals

Chromosoma (2009)

И. С. Захарова Alexander I. Shevchenko Suren M. Zakian

Genomic Imprinting

Imprinting (psychology)

Dosage compensation

Developmental Biology

10.1007/s00412-009-0206-8

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Citations (41)

Poluchenie geneticheski-modifitsirovannoy linii plyuripotentnykh stvolovykh kletok cheloveka, ekspressiruyushchey HIF-faktor, indutsiruemyy gipoksiey

Genes and Cells (2017)

И. С. Захарова Ànna Smirnova M. K. Zhiven Alexander I. Shevchenko Э. В. Григорьева

10.23868/gc120906

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Citations (0)

Extraembryonic endoderm stem cell lines from common voles of the genus Microtus

Russian Journal of Genetics (2008)

Alexander I. Shevchenko V. V. Demina N. A. Mazurok Antonina I. Zhelezova Yaroslav R. Efremov

XIST

10.1134/s1022795408110057

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Citations (9)

Human Induced Pluripotent Stem Cells Derived from Fetal Neural Stem Cells Successfully Undergo Directed Differentiation into Cartilage

Stem Cells and Development (2010)

S. P. Medvedev Э. В. Григорьева Alexander I. Shevchenko Anastasia A. Malakhova Elena Vladimirovna Dementyeva

Induced pluripotent stem (iPS) cells can be derived from a wide range of somatic cells via overexpression of a set of specific genes. With respect to their properties, iPS cells closely resemble embryonic stem cells. Because of their main property, pluripotency, iPS cells have excellent prospects for use in substitutive cell therapy; however, the methods of directed differentiation of iPS cells have not been yet sufficiently elaborated. In this work, we derived human iPS cells from fetal neural stem (FNS) cells by transfection with a polycistronic plasmid vector carrying the mouse Oct4, Sox2, Klf4, and c-Myc genes or a plasmid expressing the human OCT4 gene. We have shown that human FNS cells can be effectively reprogrammed despite a low transfection level (10%–15%) and that the use of 2-propylvaleric (valproic) acid and BIX-01294 increases the yield of iPS cell clones to ∼7-fold. Further, transient expression of OCT4 alone is sufficient for reprogramming. The iPS cells obtained express all the major markers of embryonic stem cells and are able to differentiate in vitro into ectodermal, mesodermal, and endodermal derivatives. In addition, we have found that the human iPS cells derived from FNS cells can be successfully subjected to in vitro directed chondrogenic differentiation to form functional cartilaginous tissue.

Reprogramming

KOSR

KLF4

Embryoid body

Directed differentiation

Induced stem cells

10.1089/scd.2010.0249

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Citations (86)

Difference between random and imprinted X inactivation in common voles

Chromosoma (2010)

Elena Vladimirovna Dementyeva Alexander I. Shevchenko O. V. Anopriyenko N. A. Mazurok Eugeny A. Elisaphenko

Genomic Imprinting

XIST

Skewed X-inactivation

Imprinting (psychology)

10.1007/s00412-010-0277-6

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Citations (12)

Induced pluripotent stem cell line ICGi037-A, obtained by reprogramming peripheral blood mononuclear cells from a patient with familial hypercholesterolemia due to heterozygous p.Trp443Arg mutations in LDLR

Stem Cell Research (2022)

И. С. Захарова Alexander I. Shevchenko Narek A. Tmoyan Eugeny A. Elisaphenko Alexander Kalinin

Familial hypercholesterolemia (FH) is an autosomal dominant disorder increasing premature cardiovascular diseases risk due to atherosclerosis. Pathogenic mutations in the LDLR gene cause most FH cases. Available treatments are effective not for all LDLR mutations. Testing drugs on FH cell models help develop new efficient treatments. We obtained an iPSC line from peripheral blood mononuclear cells of the patient with heterozygous p.Trp443Arg LDLR mutation. The iPSCs with confirmed patient-specific mutations express pluripotency markers, spontaneously differentiate into three germ layers and demonstrate normal karyotype. Patient-specific iPSCs-derived hepatocyte-like and endothelial cells are promising to develop new targeted therapies for FH.

Reprogramming

Germ layer

Genetic disorder

10.1016/j.scr.2022.102703

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Citations (4)

Alternative dominance of the parental genomes in hybrid cells generated through the fusion of mouse embryonic stem cells with fibroblasts

Scientific Reports (2017)

N. M. Matveeva Veniamin Fishman И. С. Захарова Alexander I. Shevchenko Inna E. Pristyazhnyuk

For the first time, two types of hybrid cells with embryonic stem (ES) cell-like and fibroblast-like phenotypes were produced through the fusion of mouse ES cells with fibroblasts. Transcriptome analysis of 2,848 genes differentially expressed in the parental cells demonstrated that 34-43% of these genes are expressed in hybrid cells, consistent with their phenotypes; 25-29% of these genes display intermediate levels of expression, and 12-16% of these genes maintained expression at the parental cell level, inconsistent with the phenotype of the hybrid cell. Approximately 20% of the analyzed genes displayed unexpected expression patterns that differ from both parents. An unusual phenomenon was observed, namely, the illegitimate activation of Xist expression and the inactivation of one of two X-chromosomes in the near-tetraploid fibroblast-like hybrid cells, whereas both Xs were active before and after in vitro differentiation of the ES cell-like hybrid cells. These results and previous data obtained on heterokaryons suggest that the appearance of hybrid cells with a fibroblast-like phenotype reflects the reprogramming, rather than the induced differentiation, of the ES cell genome under the influence of a somatic partner.

Reprogramming

Cell fusion

XIST

Cell type

Somatic fusion

10.1038/s41598-017-18352-4

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Citations (8)

Diverse developmental strategies of X chromosome dosage compensation in eutherian mammals

The International Journal of Developmental Biology (2019)

Alexander I. Shevchenko Elena Vladimirovna Dementyeva И. С. Захарова Suren M. Zakian

In eutherian mammals, dosage compensation arose to balance X-linked gene expression between sexes and relatively to autosomal gene expression in the evolution of sex chromosomes. Dosage compensation occurs in early mammalian development and comprises X chromosome upregulation and inactivation that are tightly coordinated epigenetic processes. Despite a uniform principle of dosage compensation, mechanisms of X chromosome inactivation and upregulation demonstrate a significant variability depending on sex, developmental stage, cell type, individual, and mammalian species. The review focuses on relationships between X chromosome inactivation and upregulation in mammalian early development.

Dosage compensation

Gene dosage

Skewed X-inactivation

XIST

10.1387/ijdb.180376as

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Citations (9)