Seung-Ju Cha

University of Ulsan

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Jeong‐Woo Park

Korea Institute of Radiological and Medical Sciences

Jeong-Wan Do

National Fisheries Research and Development Institute

Chang-Hoon Mun

Wha-Ja Cho

University of Ulsan

Jong-Deuk Bang

Ho‐Sung Kang

Pusan National University

Jeongyun Choi

Colorado State University

Sang-Gyu Sohn

National Fisheries Research and Development Institute

Eun‐Hee Lee

Pusan National University

Wha-Ja Cho

Cooperative Institutions

University of Ulsan

Pusan National University

Seoul National University

Kyungpook National University

Korea Advanced Institute of Science and Technology

National Fisheries Research and Development Institute

Chonnam National University

Dong-A University

Gyeongsang National University

Jeonbuk National University

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Molecular cloning of a novel GTP‐binding protein induced in fish cells by rhabdovirus infection

FEBS Letters (1998)

Eun‐Hee Lee Hyun Ju Kim Jeong-Jae Park Jeongyun Choi Wha-Ja Cho

We have cloned and sequenced a cDNA encoding GTP-binding protein from a fish cell, CHSE-214. The clone was 1493 bp long and contained an open reading frame encoding 364 amino acids. It has the five sequence motifs G1-G5 that are conserved in all GTP-binding proteins. Its amino acid sequences are strikingly different from those of the well-characterized G-proteins. However, sequences closely related to this protein are found in various kinds of species including human, Arabidopsis, Drosophila and archaebacteria, suggesting a novel subfamily within the superfamily of the GTP-binding proteins. Northern analysis indicates that this gene is constitutively expressed at a low level in normal cells but is induced by fish rhabdovirus infection at about 24 h post infection and disappears thereafter. Based on these observations, we propose that this protein represents an evolutionarily conserved novel subfamily of GTP-binding proteins which may play an important role in fish rhabdovirus infection.

Cloning (programming)

10.1016/s0014-5793(98)00641-3

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Rapid Diagnosis of Iridovirus Infection by Polymerase Chain Reaction

Journal of fish pathology (1998)

Seung-Ju Cha Jeong-Wan Do Hyun-Ju Kim Wha-Ja Cho Chang-Hoon Mun

For rapid detection of iridovirus infection, a PCR-based diagnostic method was developed. The genomic DNA from mortality-associated iridovirus was cloned into pUC19 vector. The nucleotide sequences of these clones were compared with sequences of other genes from EMBL/GenBank databank. Based on the nucleotide sequences, PCR primers were prepared and used for PCR. The DNA amplification did not occur from the normal fish cells. In contrast, DNA was amplified from the iridovirus-infected fish cells and purified iridovirus. These results suggest that mortality-associated iridovirus can be detected from virus-infected cells within short time and this PCR-based diagnostic system provides a simple and accurate method for detecting the presence of iridovirus infection.

Iridovirus

Primer (cosmetics)

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A Novel 90-kDa Stress Protein Induced in Fish Cells by Fish Rhabdovirus Infection

Biochemical and Biophysical Research Communications (1997)

Wha-Ja Cho Seung-Ju Cha Jeong-Wan Do Jeongyun Choi Joo-Yang Lee

Molecular mass

10.1006/bbrc.1997.6387

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Comparisons of Immunological Characteristics of Iridoviruses Isolated from Cultured Flounder in Korea

Journal of fish pathology (1998)

Jeong-Wan Do Seung-Ju Cha Hyunju Kim Wha-Ja Cho Chang-Hoon Mun

In order to determine whether the tumor-inducing iridoviruses and the mortality-associated iridoviruses from cultured marine fish in Korea belong to same type, we compared the immunological characteristics of these viruses. The electrophoretical pattern of structural proteins of the tumor-inducing was different from that of the mortality-associated iridoviruses. The antigenicity of structural proteins of these viruses were identified by Western blotting using two monoclonal antibodies against tumor-inducing iridovirus. Two monoclonal antibodies recognized a 150 kDa structural protein of tumor-inducing iridoviruses showed. However, the structural proteins of the mortality-associated iridoviruses did not react with these monoclonal antibodies. These results demonstrate that the antigenicity of the structural proteins of tumor-inducing iridovirus is different from that of mortality-associated iridovirus, indicating that these two iridoviruses belong to different types.

Iridovirus

Antigenicity

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Determination of subcellular localization of Betanodavirus B2

Proceedings of the Korean Aquaculture Society Conference (2006)

Yeongmi Kim Seung-Ju Cha Chang-Hun Mun Jeong-Wan Do Jeong-U Park

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