Vera V. Morozova

Siberian Branch of the Russian Academy of Sciences

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Papers

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i-10 index

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Co-Authors

Nina V. Tikunova

Siberian Branch of the Russian Academy of Sciences

Artem Tikunov

Institute of Chemical Biology and Fundamental Medicine

Igor V. Babkin

Institute of Chemical Biology and Fundamental Medicine

Alevtina Bardashevа

Siberian Branch of the Russian Academy of Sciences

Yuliya Kozlova

Siberian Branch of the Russian Academy of Sciences

Ivan K. Baykov

Siberian Branch of the Russian Academy of Sciences

Elena V. Zhirakovskaya

Siberian Branch of the Russian Academy of Sciences

E. I. Ryabchikova

Institute of Chemical Biology and Fundamental Medicine

Yana A. Khlusevich

Siberian Branch of the Russian Academy of Sciences

ЕВ

Е. В. Жираковская

Siberian Branch of the Russian Academy of Sciences

Cooperative Institutions

Siberian Branch of the Russian Academy of Sciences

105

Russian Academy of Sciences

Institute of Chemical Biology and Fundamental Medicine

Novosibirsk State University

State Research Center of Virology and Biotechnology VECTOR

Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing

Institute of Bioorganic Chemistry

Institute of Cytology and Genetics

University College Cork

APC Microbiome Institute

Author Statistics

Papers

Citation

H-Index

i-10 index

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Phage vB_KlebPS_265 Active Against Resistant/MDR and Hypermucoid K2 Strains of Klebsiella pneumoniae

Viruses (2025)

Vyacheslav I. Yakubovskij Vera V. Morozova Yuliya Kozlova Artem Tikunov Valeria A. Fedorets

Klebsiella pneumoniae is an important opportunistic pathogen often resistant to antibiotics. Specific phages can be useful in eliminating infection caused by K. pneumoniae. Klebsiella phage vB_KlebPS_265 (KlebP_265) and its host strain were isolated from the sputum of a patient with Klebsiella infection. KlebP_265 was specific mainly to K. pneumoniae-type K2 strains including hypermucoid strains. Most of the hypermucoid KlebP_265-susceptible strains were antibiotic-resistant. This siphophage demonstrated good lytic activity and stability. The KlebP_265 genome was 46,962 bp and contained 88 putative genes; functions were predicted for 37 of them. No genes encoding integrases, toxins, or antibiotic resistance were found in the genome. So, KlebP_265 could potentially be a therapeutic phage. Comparative analysis indicated that KlebP_265 with the most relative Klebsiella phage DP01 formed the putative Dipiunovirus genus. Genome analysis revealed a large monophyletic group of phages related to KlebP_265 and DP01. This group is divided into two monophyletic clusters of phages forming new putative subfamilies Skatevirinae and Roufvirinae. Phylogenetic analysis showed extensive gene exchange between phages from the putative subfamilies. Horizontal transfer even involved conservative genes and led to clear genomic mosaicism, indicating multiple recombination events in the ancestral phages during evolution.

Lytic cycle

Klebsiella

Horizontal Gene Transfer

Phage therapy

10.3390/v17010083

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A Novel Podophage StenR_269 Suggests a New Family in the Class Caudoviricetes

Viruses (2023)

Vyacheslav I. Yakubovskij Vera V. Morozova Yuliya Kozlova Artem Tikunov Igor V. Babkin

Stenotrophomonas rhizophila was first discovered in soil; it is associated with the rhizosphere and capable of both protecting roots and stimulating plant growth. Therefore, it has a great potential to be used in biocontrol. The study of S. rhizophila phages is important for a further evaluation of their effect on the fitness and properties of host bacteria. A novel phage StenR_269 and its bacterial host S. rhizophila were isolated from a soil sample in the remediation area of a coal mine. Electron microscopy revealed a large capsid (~Ø80 nm) connected with a short tail, which corresponds to the podovirus morphotype. The length of the genomic sequence of the StenR_269 was 66,322 bp and it contained 103 putative genes; 40 of them encoded proteins with predicted functions, 3 corresponded to tRNAs, and the remaining 60 were identified as hypothetical ones. Comparative analysis indicated that the StenR_269 phage had a similar genome organization to that of the unclassified Xanthomonas phage DES1, despite their low protein similarity. In addition, the signature proteins of StenR_269 and DES1 had low similarity and these proteins clustered far from the corresponding proteins of classified phages. Thus, the StenR_269 genome is orphan and the analyzed data suggest a new family in the class Caudoviricetes.

Stenotrophomonas

10.3390/v15122437

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New human single chain anti-idiotypic antibody against benzo[a]pyrene

Central European Journal of Immunology (2017)

Artem E. Studennikov Valentin A. Ustinov Vera V. Morozova Nina V. Tikunova А. Н. Глушков

ENWEndNote BIBJabRef, Mendeley RISPapers, Reference Manager, RefWorks, Zotero AMA Studennikov A, Ustinov V, Morozova V, Tikunova N, Glushkov A. New human single chain anti-idiotypic antibody against benzo[a]pyrene. Central European Journal of Immunology. 2017;42(2):123-130. doi:10.5114/ceji.2017.69353. APA Studennikov, A., Ustinov, V., Morozova, V., Tikunova, N., & Glushkov, A. (2017). New human single chain anti-idiotypic antibody against benzo[a]pyrene. Central European Journal of Immunology, 42(2), 123-130. https://doi.org/10.5114/ceji.2017.69353 Chicago Studennikov, Artem E., Valentin A. Ustinov, Vera V. Morozova, Nina V. Tikunova, and Andrey N. Glushkov. 2017. "New human single chain anti-idiotypic antibody against benzo[a]pyrene". Central European Journal of Immunology 42 (2): 123-130. doi:10.5114/ceji.2017.69353. Harvard Studennikov, A., Ustinov, V., Morozova, V., Tikunova, N., and Glushkov, A. (2017). New human single chain anti-idiotypic antibody against benzo[a]pyrene. Central European Journal of Immunology, 42(2), pp.123-130. https://doi.org/10.5114/ceji.2017.69353 MLA Studennikov, Artem E. et al. "New human single chain anti-idiotypic antibody against benzo[a]pyrene." Central European Journal of Immunology, vol. 42, no. 2, 2017, pp. 123-130. doi:10.5114/ceji.2017.69353. Vancouver Studennikov A, Ustinov V, Morozova V, Tikunova N, Glushkov A. New human single chain anti-idiotypic antibody against benzo[a]pyrene. Central European Journal of Immunology. 2017;42(2):123-130. doi:10.5114/ceji.2017.69353.

clone (Java method)

10.5114/ceji.2017.69353

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Citations (2)

Genome Analysis of Epsilon CrAss-like Phages

Viruses (2024)

Igor V. Babkin Artem Tikunov Ivan K. Baykov Vera V. Morozova Nina V. Tikunova

CrAss-like phages play an important role in maintaining ecological balance in the human intestinal microbiome. However, their genetic diversity and lifestyle are still insufficiently studied. In this study, a novel CrAssE-Sib phage genome belonging to the epsilon crAss-like phage genomes was found. Comparative analysis indicated that epsilon crAss-like phages are divided into two putative genera, which were proposed to be named Epsilonunovirus and Epsilonduovirus; CrAssE-Sib belongs to the former. The crAssE-Sib genome contains a diversity-generating retroelement (DGR) cassette with all essential elements, including the reverse transcriptase (RT) and receptor binding protein (RBP) genes. However, this RT contains the GxxxSP motif in its fourth domain instead of the usual GxxxSQ motif found in all known phage and bacterial DGRs. RBP encoded by CrAssE-Sib and other Epsilonunoviruses has an unusual structure, and no similar phage proteins were found. In addition, crAssE-Sib and other Epsilonunoviruses encode conserved prophage repressor and anti-repressors that could be involved in lysogenic-to-lytic cycle switches. Notably, DNA primase sequences of epsilon crAss-like phages are not included in the monophyletic group formed by the DNA primases of all other crAss-like phages. Therefore, epsilon crAss-like phage substantially differ from other crAss-like phages, indicating the need to classify these phages into a separate family.

Lysogenic cycle

Prophage

Lytic cycle

Temperateness

Bacterial genome size

10.3390/v16040513

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Citations (2)

Oncolytic enteroviruses

Molecular Biology (2012)

Peter M. Chumakov Vera V. Morozova Igor V. Babkin И. К. Байков Netesov Sv

Coxsackievirus

Cancer Therapy

Enterovirus Infections

10.1134/s0026893312050032

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Citations (24)

IDENTIFICATION AND DETERMINATION OF RESISTANCE TO ANTIMYCOTIC FACTORS IN YEAST CLINICAL ISOLATES DURING RESPIRATORY DISEASES

Russian Journal of Infection and Immunity (2024)

И. С. Андреева Vera V. Morozova А. С. Кабанов

The prevalence of yeast infections has increased significantly over the past few decades, with resistance of isolated pathogens to antimicrobial agents becoming progressively more pressing. Commonly occurring pathogenic yeasts include genus Candida, with C. albicans species being the dominant in both superficial and invasive infectious processes. This report presents data on the identification and determination of antimycotic drug resistance for yeast isolates obtained from clinical material of a patient with chronic bronchitis and a patient with generalized fungal infection. As a result of genomic identification, the strain causing the generalized infection was identified as Candida utilis, confirming the literature data on its increasing prevalence, considered a rare pathogen of invasive processes, as a new dangerous pathogen. Antimycotic susceptibility testing revealed resistance of C. utilis strain to commonly used antifungal drugs such as ketoconazole, itraconazole and fluconazole, weak sensitivity to clotrimazole, nystatin and amphotericin B. The strain from a patient with chronic bronchitis was identified as C. africana, considered to be a C. albicans species. Excepting itraconazole, the strain showed varying sensitivity to the five antimycotic drugs used in the experiment. Both isolated yeast strains actively grew at elevated temperature, were able to form a capsule, hyphal sprouts and biofilms, features which distinguish potentially pathogenic Candida from saprotrophic strains. Combined antifungal therapy includes the use of probiotic preparations based on microorganisms that exhibit antagonism against fungi. The present work shows an effective inhibitory effect from secreted water-soluble metabolites of spore-forming bacteria Bacillus subtilis and Bacillus lichenformis, antiseptics “Octenisept” and “Chlorhexidine”, as well as rosemary and sandalwood oil extracts on growth activity of the studied pathogenic yeast strains and a typical control strain of C. albicans Y-583.

Nystatin

Chronic bronchitis

10.15789/2220-7619-iad-17630

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Influence of Caudovirales Phages on Humoral Immunity in Mice

Viruses (2021)

А. В. Чечушков Yuliya Kozlova Ivan K. Baykov Vera V. Morozova Bogdana Kravchuk

Bacteriophages are promising antibacterial agents. Although they have been recognized as bacterial viruses and are considered to be non-interacting with eukaryotic cells, there is growing evidence that phages may have a significant impact on the immune system via interactions with macrophages, neutrophils, and T-cell polarization. In this study, the influence of phages of podovirus, siphovirus, and myovirus morphotypes on humoral immunity of CD-1 mice was investigated. In addition, tissue distribution of the phages was tested in these mice. No common patterns were found either in the distribution of phages in mice or in changes in the levels of cytokines in the sera of mice once injected with phages. Importantly, pre-existing IgM-class antibodies directed against capsid proteins of phages with myovirus and siphovirus morphotypes were identified in mice before immunization. After triple immunization of CD1-mice with phages without any adjuvant, levels of anti-phage serum polyclonal IgG antibodies increased. Immunogenic phage proteins recognized by IgM and/or IgG antibodies were identified using Western blot analysis and mass spectrometry. In addition, mice serum collected after immunization demonstrated neutralizing properties, leading to a substantial decrease in infectivity of investigated phages with myovirus and siphovirus morphotypes. Moreover, serum samples collected before administration of these phages exhibited some ability to reduce the phage infectivity. Furthermore, Proteus phage PM16 with podovirus morphotype did not elicit IgM or IgG antibodies in immunized mice, and no neutralizing activities against PM16 were revealed in mouse serum samples before and after immunization.

Infectivity

Humoral immunity

Polyclonal antibodies

10.3390/v13071241

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Citations (5)

Potential for Phages in the Treatment of Bacterial Sexually Transmitted Infections

Antibiotics (2021)

Kathryn Cater Ryszard Międzybrodzki Vera V. Morozova Sławomir Letkiewicz Marzanna Łusiak-Szelachowska

Bacterial sexually transmitted infections (BSTIs) are becoming increasingly significant with the approach of a post-antibiotic era. While treatment options dwindle, the transmission of many notable BSTIs, including Neisseria gonorrhoeae, Chlamydia trachomatis, and Treponema pallidum, continues to increase. Bacteriophage therapy has been utilized in Poland, Russia and Georgia in the treatment of bacterial illnesses, but not in the treatment of bacterial sexually transmitted infections. With the ever-increasing likelihood of antibiotic resistance prevailing and the continuous transmission of BSTIs, alternative treatments must be explored. This paper discusses the potentiality and practicality of phage therapy to treat BSTIs, including Neisseria gonorrhoeae, Chlamydia trachomatis, Treponema pallidum, Streptococcus agalactiae, Haemophilus ducreyi, Calymmatobacterium granulomatis, Mycoplasma genitalium, Ureaplasma parvum, Ureaplasma urealyticum, Shigella flexneri and Shigella sonnei. The challenges associated with the potential for phage in treatments vary for each bacterial sexually transmitted infection. Phage availability, bacterial structure and bacterial growth may impact the potential success of future phage treatments. Additional research is needed before BSTIs can be successfully clinically treated with phage therapy or phage-derived enzymes.

Mycoplasma genitalium

Neisseria gonorrhoeae

Phage therapy

Shigella sonnei

Ureaplasma urealyticum

Bacillary dysentery

10.3390/antibiotics10091030

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Citations (10)

Fecal microbiome change in patients with ulcerative colitis after fecal microbiota transplantation

А. Ю. Тикунов В. В. Морозов Аlexander N. Shvalov Alevtina Bardashevа E. Shrainer

Intestinal human microbiota is a dynamic system that is under the pressures of its host organism and external factors. Microbiota disruption caused by these factors can lead to severe diseases including inflammatory and oncological diseases of the gastrointestinal tract. One of the possible approaches in managing the intestinal microbiota is fecal microbiota transplantation (FT) – transfer of the microbiota from the stool of a healthy donor to the intestinal tract of a recipient patient. Currently, this procedure is recognized as an efficacious method to normalize the intestinal microbiota mainly in inflammatory diseases of the gastrointestinal tract. In Russia, pilot studies of the effectiveness of FT in patients with ulcerative colitis have been conducted for several years, and these studies were started in Novosibirsk. The aim of this study was to assess the change of intestinal microbiome in 20 patients with ulcerative colitis after a single FT procedure. The main method is a comparative analysis of 16S ribosomal RNA sequence libraries constructed using fecal samples obtained from patients with ulcerative colitis before and after FT and sequenced on the Illumina MiSeq platform. The obtained results showed that FT led to an increase in average biodiversity in samples after FT compared to samples before FT; however, the difference was not significant. In the samples studied, the proportion of Firmicutes sequences, the major gastrointestinal microbiota of healthy people, was decreased (~32 % vs. >70 %), while the proportion of Proteobacteria sequences was increased (>9 % vs. <5 %). In some samples collected before FT, sequences of pathogenic Firmicutes and Proteobacteria were detected, including Acinetobacter spp., Enterococcusspp., Klebsiella pneumoniae, Proteus mirabilis, Staphylococcus aureus, Stenotrophomonas maltophylia, Streptococcusspp. In most cases, the proportion of such sequences after FT substantially decreased in appropriate samples. The exception was the Clostridiumdifficilesequences, which accounted for <0.5 % of the sequences in samples from almost half of the patients and after FT, the share of such C. difficilesequences was significantly reduced only in samples from three patients. It should be noted that the proportion of Lactobacillusspp. increased ten-fold and their species composition significantly expanded. According to the obtained results, a preliminary conclusion can be made that even a single FT procedure can lead to an increase in the biodiversity of the gastrointestinal microbiota in patients and to the optimization of the taxonomic composition of the microbiota.

Fecal bacteriotherapy

Human gastrointestinal tract

Source

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Antibiotic Resistance and Pathogenomics of Staphylococci Circulating in Novosibirsk, Russia

Microorganisms (2021)

Alevtina Bardashevа Artem Tikunov Yuliya Kozlova Е. В. Жираковская Valeriya Fedorets

A total of 394 strains of staphylococci found in humans and pets in Novosibirsk, Siberian Russia, were characterized in terms of antibiotic resistance and corresponding genes. Two coagulase-positive and 17 coagulase-negative species were identified. The majority of isolates, with the exception of S. haemolyticus and hospital S. epidermidis isolates, were sensitive to most of the tested antibiotics, and isolates from pets displayed the lowest level of resistance. Nevertheless, methicillin-resistant (MRS) and/or multidrug-resistant (MDR) isolates were found in all prevailed species, including coagulase-negative. A set of genes corresponding to the detected resistance was identified: mecA (beta-lactam resistance), aac(6')-Ie-aph(2″)-Ia, aph(3')-IIIa, ant(4')-Ia (aminoglycoside-modifying enzymes), ermA/ermC, and msrA (macrolide resistance). Complete genome analysis for ten MDR S. epidermidis and five MDR S. haemolyticus isolates revealed additional antibiotic resistance genes mphC, qacA/qacB, norA, dfrC/dfrG, lnuA, BseSR, and fosB. NorA, dfrC, and fosB were present in all S. epidermidis genomes, whereas mphC and msrA were identified in all S. haemolyticus ones. All investigated MDR S. epidermidis and four of five S. haemolyticus strains were moderate or strong biofilm producers, whereas multiple genes responsible for this function and for virulence and pathogenicity were identified mostly in S. epidermidis, but were less frequently represented in S. haemolyticus.

Staphylococcus haemolyticus

Coagulase

SCCmec

10.3390/microorganisms9122487

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Citations (9)