Martin Juraščík

Eindhoven University of Technology

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Jozef Markoš

Slovak University of Technology in Bratislava

Jaroslav Klein

Universidade Federal do Paraná

J. A. Teixeira

University of Minho

I. Sikula

Slovak University of Technology in Bratislava

Lucı́lia Domingues

University of Minho

J. Annus

Slovak University of Technology in Bratislava

K.J. Ptasiński

Eindhoven University of Technology

Anna Sues

Eindhoven University of Technology

A. A. Vicente

University of Minho

Pedro M. R. Guimarães

Universidade Estadual de Campinas (UNICAMP)

Cooperative Institutions

Slovak University of Technology in Bratislava

University of Minho

Eindhoven University of Technology

Institute for Biotechnology and Bioengineering

Polytechnic Institute of Bragança

Lucideon (United Kingdom)

Science and Technology Facilities Council

Central Laser Facility

Universidade Estadual Paulista (Unesp)

Universitat Politècnica de Catalunya

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Exergetic evaluation and improvement of biomass-to-synthetic natural gas conversion

Energy & Environmental Science (2009)

Martin Juraščík Anna Sues K.J. Ptasiński

Synthetic natural gas (SNG) is suggested as an important future energy carrier. The conventional route for SNG production is based on gasification of biomass to synthetic gas and the subsequent methanation of synthetic gas to SNG. This study is aimed to analyze the process units using the concept of exergy. Exergy analysis is a promising method, based on the 2nd law of thermodynamics, to analyze and improve chemical processes. In this work a detailed exergy analysis is performed for the SNG process based on woody biomass gasification. The main elements of the system are gasifier, gas cleaning, synthetic gas compression, methanation and final SNG condition. The above-mentioned process was simulated with a computer model using the flow-sheeting program Aspen Plus. Optimal values of the process conditions, particularly for the methanation reactors, are found. The internal exergy losses of different system units are evaluated. The largest internal exergy losses take place in the gasifier, methanation section and CO2 capture unit. The highest overall exergetic efficiency of 72.6% was found applying the following operating conditions: gasifier 700 °C and 1 bar; 1st methanation reactor 580 °C and 2nd methanation reactor 405 °C.

Methanation

Substitute natural gas

Wood gas generator

Exergy efficiency

10.1039/b820824g

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Citations (36)

Influence of biomass on hydrodynamics of an internal loop airlift reactor

Chemical Papers (2006)

Martin Juraščík Martin Hucík I. Sikula J. Annus Jozef Markoš

Airlift

Gluconic acid

Aspergillus niger

10.2478/s11696-006-0080-2

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Citations (8)

Exergetic efficiency of synthetic natural gas (SNG) production from biomass

KJ Krzysztof Ptasinski Martin Juraščík Anna Sues

Substitute natural gas

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Exergy analysis of synthetic natural gas production method from biomass

Energy (2009)

Martin Juraščík Anna Sues K.J. Ptasiński

Substitute natural gas

Wood gas generator

Exergy efficiency

Producer gas

10.1016/j.energy.2009.07.031

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Citations (86)

Kinetics of lactose fermentation using a recombinant Saccharomyces cerevisiae strain

Biotechnology and Bioengineering (2006)

Martin Juraščík Pedro M. R. Guimarães Jaroslav Klein Lucı́lia Domingues J. A. Teixeira

Abstract This work presents a multi‐route, non‐structural kinetic model for interpretation of ethanol fermentation of lactose using a recombinant flocculent Saccharomyces cerevisiae strain expressing both the LAC4 (coding for β ‐galactosidase) and LAC12 (coding for lactose permease) genes of Kluyveromyces lactis. In this model, the values of different metabolic pathways are calculated applying a modified Monod equation rate in which the growth rate is proportional to the concentration of a key enzyme controlling the single metabolic pathway. In this study, three main metabolic routes for S. cerevisiae are considered: oxidation of lactose, reduction of lactose (producing ethanol), and oxidation of ethanol. The main bioprocess variables determined experimentally were lactose, ethanol, biomass, and dissolved oxygen concentrations. Parameters of the proposed kinetic model were established by fitting the experimental data obtained in a small lab‐scale fermentor with the initial lactose concentrations ranging from 5 g/dm 3 to 50 g/dm 3 . A very good agreement between experimental data and simulated profiles of the main variables (lactose, ethanol, biomass, and dissolved oxygen concentrations) was achieved. © 2006 Wiley Periodicals, Inc.

Lactose permease

Kluyveromyces lactis

Ethanol Fermentation

10.1002/bit.20941

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Continuous flocculation airlift bioreactor with high cell loading : hydrodynamic and rheological aspects

Jaroslav Klein João M. Maia Martin Juraščík A. A. Vicente Lucı́lia Domingues

Centro de Engenharia Biologica, Department of Biological Engineering, University of Minho, Campus of Gualtar, 4710-057 Braga, Portugal Department of Polymer Engineering, University of Minho, Campus de Azurem, 4800 058 Guimaraes, Portugal Dept. of Chemical & Biochemical Engineering, Faculty of Chemical & Food Technology Slovak University of Technology, Radlinskeho 9, 812 37 Bratislava, Slovakia e-mail: jateixeira@deb.uminho.pt

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KINETICS OF LACTOSE FERMENTATION USING RECOMBINANT Saccharomyces cerevisiae

Martin Juraščík Pedro M. R. Guimarães Jaroslav Klein Lucı́lia Domingues J. A. Teixeira

This work presents a multi-route, nonstructural kinetic model for determination of ethanol fermentation on lactose using a recombinant S. cerevisiae flocculent strain expressing both the LAC4 (coding for β-galactosidase) and LAC12 (coding for lactose permease) genes of Kluyveromyces lactis. In this kind of modelling, the values of different metabolic pathways are calculated applying a modified Monod equation rate in which the growth rate is proportional to the concentration of a key enzyme controlling the single metabolic pathway. As a result, this type of model has a large number of parameters. In this study, three main metabolic routes for S. cerevisiae are considered: fermentation (producing ethanol), oxidation of lactose and oxidation of ethanol. The main bioprocess variables determined experimentally were lactose, ethanol and biomass concentrations, dissolved oxygen and the composition of outgoing gas.

Kluyveromyces lactis

Lactose permease

Ethanol Fermentation

Metabolic Engineering

Metabolic pathway

Bioprocess engineering

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Modeling of fermentation in an internal loop airlift bioreactor

Chemical Engineering Science (2007)

I. Sikula Martin Juraščík Jozef Markoš

Airlift

Gluconic acid

Scale-up

Draft tube

Mass transfer coefficient

10.1016/j.ces.2007.01.050

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Citations (27)

Hydrodynamic analysis of a continuous airlift bioreactor with flocculating high cell density system

Jaroslav Klein A. A. Vicente Lucı́lia Domingues Martin Juraščík J. A. Teixeira

Marie Curie Fellowship of the European Community - Programme Improving Human Research Potential, Contract number HPMF-CT-2002-01643.

Airlift

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Measurement of Mass Transfer Coefficients in Airlift Reactors with Internal Loop Using the Glucose Oxidase Method

Chemical and Biochemical Engineering Quarterly (2007)

Martin Juraščík I. Sikula Michal Rosenberg Jozef Markoš

This study deals with the measurement of volumetric mass transfer coefficients (k L a) in the internal loop airlift reactors (ILALRs) of different volumes (12 dm 3 , 40 dm 3 and 200 dm 3 ) using the glucose oxidase method. The experimental results of this work were compared with those k L a obtained for the same ILALRs by applying different other methods. Results of this work showed a good agreement with the published results obtained by applying other methods on the non-coalescent medium. The initial glucose mass concentration in the non-coalescent medium was 150 g dm -3 . Based on this work, the values of the empirical parameter in the correlation of k L a vs. the total gas hold-up, proposed by Bello et al., 1 were established.

Coalescent theory

Airlift

Glucose oxidase

Mass transfer coefficient

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