Aline Gonçalves Lio Copola

Universidade Federal de Minas Gerais

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Co-Authors

Gerluza Aparecida Borges Silva

Universidade Federal de Minas Gerais

ÉC

Érika Cristina Jorge

Universidade Federal de Minas Gerais

Júlia Meireles Nogueira

Universidade Federal de Minas Gerais

ÍG

Íria Gabriela Dias dos Santos

Universidade Federal de Minas Gerais

Erika Cristina Jorge

Universidade Federal de Minas Gerais

Alinne C. Costa

University Alto Vale do Rio do Peixe

João Paulo Ferreira Santos

Instituto Superior de Educação e Ciências

Luiz Lehmann Coutinho

Universidade de São Paulo

Luciana de Oliveira Andrade

Universidade Federal de Minas Gerais

Ana Elisa Antunes dos Santos

Universidade Federal de Minas Gerais

Cooperative Institutions

Universidade Federal de Minas Gerais

Federal Center for Technological Education of Minas Gerais

Universidade de São Paulo

Centro Universitário de Belo Horizonte

Universidade Federal de São Carlos

Brazilian Agricultural Research Corporation

Iowa State University

Universidade Federal de Lavras

Technische Universität Berlin

Universidade Estadual de Campinas (UNICAMP)

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SAP30 Gene Is a Probable Regulator of Muscle Hypertrophy in Chickens

Frontiers in Genetics (2021)

Bruna Petry Gabriel Costa Monteiro Moreira Aline Gonçalves Lio Copola Marcela Maria de Souza Fernanda Cristina da Veiga

Animals with muscle hypertrophy phenotype are targeted by the broiler industry to increase the meat production and the quality of the final product. Studies characterizing the molecular machinery involved with these processes, such as quantitative trait loci studies, have been carried out identifying several candidate genes related to this trait; however, validation studies of these candidate genes in cell culture is scarce. The aim of this study was to evaluate SAP30 as a candidate gene for muscle development and to validate its function in cell culture in vitro. The SAP30 gene was downregulated in C2C12 muscle cell culture using siRNA technology to evaluate its impact on morphometric traits and gene expression by RNA-seq analysis. Modulation of SAP30 expression increased C2C12 myotube area, indicating a role in muscle hypertrophy. RNA-seq analysis identified several upregulated genes annotated in muscle development in treated cells (SAP30-knockdown), corroborating the role of SAP30 gene in muscle development regulation. Here, we provide experimental evidence of the involvement of SAP30 gene as a regulator of muscle cell hypertrophy.

Candidate gene

C2C12

10.3389/fgene.2021.709937

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Citations (2)

Morphological characteristics of the neonate of the white-eared opossum (Didelphis albiventris Lund, 1840) (Metatheria: Didelphidae)

Brazilian Journal of Biological Sciences (2015)

B. M. Bertasoli A. C. Santos Thaís Borges Lessa Íria Gabriela Dias dos Santos Aline Gonçalves Lio Copola

Didelphis

Bandicoot

White (mutation)

Source

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Citations (3)

Investigation of bioactive nanofiber-based scaffolds for cultivated meat.

Authorea (Authorea) (2022)

Ana Elisa Antunes dos Santos Tiago Cotta João Paulo Ferreira Santos Juliana Sofia Fonseca Camargos Ana Carolina Correia do Carmo

Synthetic polymers scaffolds often need to be coated with extracellular matrix (ECM) proteins to improve cell adhesion. For cultivated meat applications, coating should be avoided since it is necessary to eliminate expensive and animal-derived components. As cellulose acetate nanofibers is a low-cost cellulose-derived material, that induces cell adhesion and proliferation, we investigated its use associated with a bioactive annatto extract, a food-dye and potential meat preservative, as scaffolds for cultivated meat. Here, the bioactive electrospun nanofibers were evaluated through morphological, mechanical and biological characterizations. The results revealed that the scaffolds were porous with no specific alignment and average fiber diameter of 420±212 nm. Molecular analyzes revealed that in contrast to cellulose acetate scaffold, annatto-loaded cellulose acetate scaffold favor a proliferative state of C2C12 mouse skeletal myoblasts. SEM microscopy images suggests that the nanofiber substrates can sustain long-term culture of the cells, up to 28 days. These results suggest that the combination of cellulose acetate fibers loaded with annatto extract may be an interesting economical alternative for support long-term muscle cells culture with potential application as a scaffold for cultivated meat and muscle tissue engineering.

Cellulose acetate

C2C12

10.22541/au.166062979.98859184/v1

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Citations (2)

Additional file 2 of Transcriptomic characterization of the molecular mechanisms induced by RGMa during skeletal muscle nuclei accretion and hypertrophy

Figshare (2022)

Aline Gonçalves Lio Copola Íria Gabriela Dias dos Santos Luiz Lehmann Coutinho Luiz‐Eduardo Del‐Bem Paulo Henrique Almeida Campos-Júnior

Additional file 2: Supplementary file 2. Differentially expressed genes modulated by RGMa treatment during the differentiation of C2C12 cell.

Characterization

10.6084/m9.figshare.19321199

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Citations (0)

Random cellulose acetate nanofibers: a breakthrough for cultivated meat production

Frontiers in Nutrition (2024)

Ana Elisa Antunes dos Santos Jorge Luís Guadalupe Juliano Douglas Silva Albergaria Itallo Augusto Almeida Amanda Maria Siqueira Moreira

Overcoming the challenge of creating thick, tissue-resembling muscle constructs is paramount in the field of cultivated meat production. This study investigates the remarkable potential of random cellulose acetate nanofibers (CAN) as a transformative scaffold for muscle tissue engineering (MTE), specifically in the context of cultivated meat applications. Through a comparative analysis between random and aligned CAN, utilizing C2C12 and H9c2 myoblasts, we unveil the unparalleled capabilities of random CAN in facilitating muscle differentiation, independent of differentiation media, by exploiting the YAP/TAZ-related mechanotransduction pathway. In addition, we have successfully developed a novel process for stacking cell-loaded CAN sheets, enabling the production of a three-dimensional meat product. C2C12 and H9c2 loaded CAN sheets were stacked (up to four layers) to form a ~300–400 μm thick tissue 2 cm in length, organized in a mesh of uniaxial aligned cells. To further demonstrate the effectiveness of this methodology for cultivated meat purposes, we have generated thick and viable constructs using chicken muscle satellite cells (cSCs) and random CAN. This groundbreaking discovery offers a cost-effective and biomimetic solution for cultivating and differentiating muscle cells, forging a crucial link between tissue engineering and the pursuit of sustainable and affordable cultivated meat production.

C2C12

10.3389/fnut.2023.1297926

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Citations (5)

Additional file 1 of Transcriptomic characterization of the molecular mechanisms induced by RGMa during skeletal muscle nuclei accretion and hypertrophy

Figshare (2022)

Aline Gonçalves Lio Copola Íria Gabriela Dias dos Santos Luiz Lehmann Coutinho Luiz‐Eduardo Del‐Bem Paulo Henrique Almeida Campos-Júnior

Additional file1: Supplementary file 1. Differentially expressed transcripts modulated by RGMa treatment during the differentiation of C2C12 cells.

Characterization

10.6084/m9.figshare.19321196

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Citations (0)

Transcriptomic characterization of the molecular mechanisms induced by RGMa during skeletal muscle nuclei accretion and hypertrophy

BMC Genomics (2022)

Aline Gonçalves Lio Copola Íria Gabriela Dias dos Santos Luiz Lehmann Coutinho Luiz‐Eduardo Del‐Bem Paulo Henrique Almeida Campos-Júnior

The repulsive guidance molecule a (RGMa) is a GPI-anchor axon guidance molecule first found to play important roles during neuronal development. RGMa expression patterns and signaling pathways via Neogenin and/or as BMP coreceptors indicated that this axon guidance molecule could also be working in other processes and diseases, including during myogenesis. Previous works from our research group have consistently shown that RGMa is expressed in skeletal muscle cells and that its overexpression induces both nuclei accretion and hypertrophy in muscle cell lineages. However, the cellular components and molecular mechanisms induced by RGMa during the differentiation of skeletal muscle cells are poorly understood. In this work, the global transcription expression profile of RGMa-treated C2C12 myoblasts during the differentiation stage, obtained by RNA-seq, were reported.RGMa treatment could modulate the expression pattern of 2,195 transcripts in C2C12 skeletal muscle, with 943 upregulated and 1,252 downregulated. Among them, RGMa interfered with the expression of several RNA types, including categories related to the regulation of RNA splicing and degradation. The data also suggested that nuclei accretion induced by RGMa could be due to their capacity to induce the expression of transcripts related to 'adherens junsctions' and 'extracellular-cell adhesion', while RGMa effects on muscle hypertrophy might be due to (i) the activation of the mTOR-Akt independent axis and (ii) the regulation of the expression of transcripts related to atrophy. Finally, RGMa induced the expression of transcripts that encode skeletal muscle structural proteins, especially from sarcolemma and also those associated with striated muscle cell differentiation.These results provide comprehensive knowledge of skeletal muscle transcript changes and pathways in response to RGMa.

C2C12

10.1186/s12864-022-08396-w

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Citations (5)

Bioactive cellulose acetate nanofiber loaded with annatto support skeletal muscle cell attachment and proliferation

Frontiers in Bioengineering and Biotechnology (2023)

Ana Elisa Antunes dos Santos Tiago Cotta João Paulo Ferreira Santos Juliana Sofia Fonseca Camargos Ana Carolina Correia do Carmo

Electrospinning emerged as a promising technique to produce scaffolds for cultivated meat in function of its simplicity, versatility, cost-effectiveness, and scalability. Cellulose acetate (CA) is a biocompatible and low-cost material that support cell adhesion and proliferation. Here we investigated CA nanofibers, associated or not with a bioactive annatto extract (CA@A), a food-dye, as potential scaffolds for cultivated meat and muscle tissue engineering. The obtained CA nanofibers were evaluated concerning its physicochemical, morphological, mechanical and biological traits. UV-vis spectroscopy and contact angle measurements confirmed the annatto extract incorporation into the CA nanofibers and the surface wettability of both scaffolds, respectively. SEM images revealed that the scaffolds are porous, containing fibers with no specific alignment. Compared with the pure CA nanofibers, CA@A nanofibers showed increased fiber diameter (420 ± 212 nm vs. 284 ± 130 nm). Mechanical properties revealed that the annatto extract induces a reduction of the stiffness of the scaffold. Molecular analyses revealed that while CA scaffold favored C2C12 myoblast differentiation, the annatto-loaded CA scaffold favored a proliferative state of these cells. These results suggest that the combination of cellulose acetate fibers loaded with annatto extract may be an interesting economical alternative for support long-term muscle cells culture with potential application as scaffold for cultivated meat and muscle tissue engineering.

Cellulose acetate

Electrospinning

C2C12

10.3389/fbioe.2023.1116917

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Citations (17)

Acetate cellulose fibrous scaffold is suitable for cultivated fat production

Current Research in Food Science (2024)

Amanda Maria Siqueira Moreira Júlia Meireles Nogueira Jade Carceroni Jorge Luís Guadalupe Ana Elisa Antunes dos Santos

Cellulose acetate

10.1016/j.crfs.2024.100903

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