Marı́a Angélica Santana

Universidad Autónoma del Estado de Morelos

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Gustavo Romay

Texas A&M University

Oscar Ramírez-Pliego

Universidad Autónoma del Estado de Morelos

Annika Gillis

UCLouvain

Denis Thieffry

École Normale Supérieure

Juan Matehus

Instituto de Estudios Avanzados

Dorys T. Chirinos

Universidad Técnica de Manabí

Margarita Rodríguez

University of Miami

Fernando Esquivel‐Guadarrama

Universidad Autónoma del Estado de Morelos

Otoniel Rodríguez-Jorge

Universidad Autónoma del Estado de Morelos

Morgane Thomas‐Chollier

Institut de Biologie de l'École Normale Supérieure

Cooperative Institutions

Universidad Autónoma del Estado de Morelos

Universidade de São Paulo

Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo

Universidad Nacional Autónoma de México

Clinics Hospital of Ribeirão Preto

Instituto do Coração

Universidade Federal da Bahia

Inserm

Centre National de la Recherche Scientifique

Universidade Estadual de Campinas (UNICAMP)

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Rotavirus Infection Activates Dendritic Cells from Peyer's Patches in Adult Mice

Journal of Virology (2009)

Delia Vanesa López-Guerrero Selene Meza‐Perez Oscar Ramírez-Pliego Marı́a Angélica Santana Gerardo Pável Espino-Solís

This study used an in vivo mouse model to analyze the response of dendritic cells (DCs) in Peyer's patches (PPs) within the first 48 h of infection with the wild-type murine rotavirus EDIM (EDIMwt). After the infection, the absolute number of DCs was increased by 2-fold in the PPs without a modification of their relative percentage of the total cell number. Also, the DCs from PPs of infected mice showed a time-dependent migration to the subepithelial dome (SED) and an increase of the surface activation markers CD40, CD80, and CD86. This response was more evident at 48 h postinfection (p.i.) and depended on viral replication, since DCs from PPs of mice inoculated with UV-treated virus did not show this phenotype. As a result of the activation, the DCs showed an increase in the expression of mRNA for the proinflammatory cytokines interleukin-12/23p40 (IL-12/23p40), tumor necrosis factor alpha (TNF-α), and beta interferon (IFN-β), as well as for the regulatory cytokine IL-10. These results suggest that, a short time after rotavirus infection, the DCs from PPs play a critical role in controlling the infection and, at the same time, avoiding an excessive inflammatory immune response.

CD80

Proinflammatory cytokine

CD86

10.1128/jvi.02640-08

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Citations (39)

Dornase alfa improves the health‐related quality of life among Brazilian patients with cystic fibrosis—A one‐year prospective study

Pediatric Pulmonology (2010)

Tatiana Rozov Viviane Ziebell de Oliveira Marı́a Angélica Santana Fabíola Villac Adde Rita Mendes

Health-related quality of life (HRQOL) measurements provide valuable information about the psychological and social impact of treatment on patients with cystic fibrosis (CF). This study evaluated the HRQOL of Brazilian patients with CF and assessed the changes in HRQOL domains over 1 year after dornase alfa (Pulmozyme) introduction.One hundred fifty-six stable patients with CF and 89 caregivers answered the Portuguese-validated version of the Cystic Fibrosis Questionnaire-Revised (CFQ-R) at baseline (T(0)), and at 3 (T(1)), 6 (T(2)), 9 (T(3)), and 12 (T(4)) months of follow-up. Eighteen patients were excluded because they did not fulfill the inclusion criteria. The patients were analyzed in two groups: those aged 6-11 years and those aged 14 years and older. ANOVA for observed repeated results and the last observation carried forward (LOCF) method for missing data were used for the statistical analysis.After 1 year of follow-up, there was significant improvement in respiratory symptoms (T(4) - T(0) = 8.1; 95% confidence interval (95% CI) = [2.1;14.0]; effect size (ES) = 0.35; P < 0.001), Emotional Functioning (T(4) - T(0) = 5.6; 95% CI = [1.1;10.1]; ES = 0.31; P < 0.05), Social Functioning (T(4) - T(0) = 6.0; 95% CI = [1.3;11.7]; ES = 0.31; P < 0.05), Body Image (T(4) - T(0) = 11.9; 95% CI = [4.1;19.7]; ES = 0.42; P < 0.05), and Treatment Burden (T(4) - T(0) = 5.3; 95% CI = [0.3;10.3]; ES = 0.24; P < 0.05) domains in the younger group. A significant improvement in Role Functioning (T(4) - T(0) = 6.1; 95% CI = [1.1;11.1]; ES = 0.40; P < 0.05), Body Image (T(4) - T(0) = 12.6; 95% CI = [3.5;21.7]; ES = 0.46; P < 0.05), and Weight (T(4) - T(0) = 11.7; 95% CI = [1.8;21.6]; ES = 0.40; P < 0.05) was obtained in the older group. The caregivers' CFQ-R showed improvements in the Digestive Symptoms (T(4) - T(0) = 5.5; 95% CI = [1.5;9.4]; ES = 0.30; P < 0.05), Respiratory Symptoms (T(4) - T(0) = 7.6; 95% CI = [3.9;11.4]; ES = 0.48; P < 0.05), and Weight (T(4) - T(0) = 10.1; 95% CI = [1.6;18.6]; ES = 0.26; P < 0.05) domains.The introduction of dornase alfa improved the HRQL of the patients with CF during the first year of treatment.

10.1002/ppul.21267

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Citations (21)

Efecto de tres herbicidas pre-emergentes en el establecimiento en campo de plantas in vitro de yuca (Manihot esculenta Crantz)

Gustavo Romay Marı́a Angélica Santana Juan Matehus

Entre los principales problemas para el desarrollo del cultivo de la yuca (Manihot esculenta Crantz) en Venezuela esta la carencia de buen material de siembra. Tradicionalmente, la yuca se propaga por estacas, siendo esto una fuente importante en la diseminacion de plagas y enfermedades. El uso de plantas in vitro permite obtener material de siembra sano y vigoroso; sin embargo, su adaptacion en campo requiere mayor atencion en el control de malezas o arvenses. El presente trabajo evaluo el efecto de 3 herbicidas, fluometuron, S-metolacloro, pendimetalin, en comparacion con el desyerbe manual, durante el establecimiento en campo de plantas in vitro de yuca. Los ensayos se realizaron en San Pablo, Edo. Yaracuy. Se uso un diseno de bloques al azar con cuatro repeticiones. Aunque los resultados no mostraron diferencias significativas en el porcentaje de supervivencia de las plantulas, su crecimiento fue significativamente superior (p <0,05) en parcelas tratadas con fluometuron y S-metolacloro con respecto al control manual. No se encontro diferencias entre tratamientos en el control general de arvenses, pero el S-metolacloro mostro mejor control de especies de hoja ancha. Se recomienda usar S-metolacloro y fluometuron el mismo dia del transplante para mejorar la competitividad de las plantulas.

Manihot esculenta

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Flagellin is a Th1 polarizing factor for human CD4+ T cells and induces protection in a murine neonatal vaccination model of rotavirus infection

Vaccine (2018)

Rosario Guadalupe Labastida-Conde Oscar Ramírez-Pliego Mercedes Peleteiro Delia Vanessa López-Guerrero O. Badillo

Flagellin

TLR5

10.1016/j.vaccine.2018.06.005

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Characterization of a murine model of endothelial dysfunction induced by chronic intraperitoneal administration of angiotensin II

Scientific Reports (2021)

Celeste Trejo-Moreno Enrique Jiménez‐Ferrer Gabriela Castro-Martínez Marisol Méndez-Martínez Marı́a Angélica Santana

Abstract Endothelial dysfunction (ED) is a key factor for the development of cardiovascular diseases. Due to its chronic, life-threatening nature, ED only can be studied experimentally in animal models. Therefore, this work was aimed to characterize a murine model of ED induced by a daily intraperitoneal administration of angiotensin II (AGII) for 10 weeks. Oxidative stress, inflammation, vascular remodeling, hypertension, and damage to various target organs were evaluated in treated animals. The results indicated that a chronic intraperitoneal administration of AGII increases the production of systemic soluble VCAM, ROS and ICAM-1 expression, and the production of TNFα, IL1β, IL17A, IL4, TGFβ, and IL10 in the kidney, as well as blood pressure levels; it also promotes vascular remodeling and induces non-alcoholic fatty liver disease, glomerulosclerosis, and proliferative retinopathy. Therefore, the model herein proposed can be a representative model for ED; additionally, it is easy to implement, safe, rapid, and inexpensive.

Glomerulosclerosis

Intraperitoneal injection

Endothelial Dysfunction

10.1038/s41598-021-00676-x

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A first-year dornase alfa treatment impact on clinical parameters of patients with cystic fibrosis: the Brazilian cystic fibrosis multicenter study

Revista Paulista de Pediatria (2013)

Tatiana Rozov Fernando Antônio A. e Silva Marı́a Angélica Santana Fabíola Villac Adde Rita Mendes

OBJECTIVE: To describe the clinical impact of the first year treatment with dornase alfa, according to age groups, in a cohort of Brazilian Cystic Fibrosis (CF) patients. METHODS: The data on 152 eligible patients, from 16 CF reference centers, that answered the medical questionnaires and performed laboratory tests at baseline (T0), and at six (T2) and 12 (T4) months after dornase alfa initiation, were analyzed. Three age groups were assessed: six to 11, 12 to 13, and >14 years. Pulmonary tests, airway microbiology, emergency room visits, hospitalizations, emergency and routine treatments were evaluated. Student's t-test, chi-square test and analysis of variance were used when appropriated. RESULTS: Routine treatments were based on respiratory physical therapy, regular exercises, pancreatic enzymes, vitamins, bronchodilators, corticosteroids, and antibiotics. In the six months prior the study (T0 phase), hospitalizations for pulmonary exacerbations occurred in 38.0, 10.0 and 61.4% in the three age groups, respectively. After one year of intervention, there was a significant reduction in the number of emergency room visits in the six to 11 years group. There were no significant changes in forced expiratory volume in one second (VEF1), in forced vital capacity (FVC), in oxygen saturation (SpO2), and in Tiffenau index for all age groups. A significant improvement in Shwachman-Kulczychi score was observed in the older group. In the last six months of therapy, chronic or intermittent colonization by P. aeruginosa was detected in 75.0, 71.4 and 62.5% of the studied groups, respectively, while S. aureus colonization was identified in 68.6, 66.6 and 41.9% of the cases. CONCLUSIONS: The treatment with dornase alfa promoted the maintenance of pulmonary function parameters and was associated with a significant reduction of emergency room visits due to pulmonary exacerbations in the six to 11 years age group, with better clinical scores in the >14 age group, one year after the intervention.

10.1590/s0103-05822013000400002

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Citations (4)

Metabolic alterations impair differentiation and effector functions of CD8+ T cells

Frontiers in Immunology (2022)

Antonio Bensussen Marı́a Angélica Santana Otoniel Rodríguez-Jorge

CD8+ T lymphocytes are one of the main effector cells of the immune system, they protect the organism against intracellular threats such as viruses and bacteria, as well as neoplasms. It is currently well established that CD8+ T cells have distinct immune responses, given by their phenotypes Tc1, Tc2, Tc17, and TcReg. The cellular plasticity of such phenotypes depends on the presence of different combinations of cytokines in the extracellular medium. It is known that metabolic imbalances play an important role in immune response, but the precise role of metabolic disturbances on the differentiation and function of CD8+ T cells, however, has not been explored. In this work, we used a computational model to explore the potential effect of metabolic alterations such as hyperglycemia, high alcohol consumption, dyslipidemia, and diabetes on CD8+ T cell differentiation. Our model predicts that metabolic alterations preclude the effector function of all CD8+ T cell phenotypes except for TcReg cells. It also suggests that such inhibition originates from the increase of reactive oxygen species in response to metabolic stressors. Finally, we simulated the outcome of treating metabolic-inhibited CD8+ T cells with drugs targeting key molecules such as mTORC1, mTORC2, Akt, and others. We found that overstimulation of mTORC2 may restore cell differentiation and functions of all effector phenotypes, even in diabetic patients. These findings highlight the importance of our predictive model to find potential targets to strengthen immunosuppressed patients in chronic diseases, like diabetes.

10.3389/fimmu.2022.945980

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What it takes to become an effector T cell: The process, the cells involved, and the mechanisms

Journal of Cellular Physiology (2003)

Marı́a Angélica Santana Yvonne Rosenstein

Abstract When activated, CD4 + T cells differentiate into two major sub‐populations differing in their profiles of secreted cytokines. Type One, or TH1, cells secrete IL‐2, IFNγ, and TNFβ and mediate a cellular immune response. Type Two, or TH2, cells secrete IL‐4, IL‐5, IL‐6, IL‐10, and IL‐13 and potentiate a humoral response. The nature of any specific immune response depends on the interaction of antigen‐presenting cells and T cells. The role of antigen‐presenting cells is to respond to the nature of the immune challenge and signal differentiation of CD4 + T cells. A number of factors are involved in the effector phenotype of T cells—nature and affinity of antigen, co‐receptors signals, and cytokine environment. T‐cell differentiation is a complex process comprising four defined developmental stages: activation of particular cytokine genes, commitment of the cells, silencing of the opposing cytokine genes, and stabilization of the phenotype. In each of these stages, the cells respond to the products of many signaling cascades from many membrane‐bound receptors. The stages in development are mediated by different molecular mechanisms, involving control of gene expression and chromatin remodeling. This review centers on the factors, cellular interactions, and molecular mechanisms involved in the maturation of naïve CD4 + T lymphocytes into fully effector cells. © 2003 Wiley‐Liss, Inc.

10.1002/jcp.10258

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Citations (95)

Multiplicación in vitro de ocumo y taro

Agronomía Tropical (2006)

Juan Matehus Gustavo Romay Marı́a Angélica Santana

espanolEl ocumo, Xanthosoma sagittifolium (L.) Schott, y el taro, Colocasia esculenta (L.) Schott son plantas herbaceas de la familia de las Araceas. Son plantas perennes que crecen en zonas tropicales, y cuya importancia radica en el consumo del cormo o tallo subterraneo modificado como organo de reserva. El metodo de multiplicacion comunmente utilizado es por fracciones vegetativas, lo que conlleva a una baja tasa de multiplicacion y a la dispersion de enfermedades. La importancia del establecimiento y multiplicacion in vitro de plantas de ambas especies con el objeto de producir semilla libre de patogenos. El objetivo del trabajo fue estudiar las condiciones optimas para la multiplicacion in vitro de plantas de ocumo y taro. Cormos de ambas especies fueron sometidos a desinfeccion superficial con detergentes liquidos, alcohol y cloro comercial (2,5%). Para el establecimiento inicial de las especies, los meristemas fueron colocados en medio MS-2% sacarosa y concentraciones hormonales de 0,1 mg l-1 bencil aminopurina (BAP) y 0,01 mg l-1 acido naftalenacetico (ANA). En cuanto a la proliferacion de brotes, se disenaron medios de cultivo con 1 mg l-1 BAP, 2 mg l-1 BAP, 1 mg l-1 BAP–3 mg l-1 AG3, 1 mg l-1 BAP–0,05 mg l-1 AG3–0,02 mg l-1 ANA y 1 mg l-1 BAP–0,5 mg l-1 AG3–0,02 mg l-1 ANA. Los resultados mostraron que la mejor tasa de proliferacion se logra con el medio MS-2% sacarosa-1 mg l-1 BAP con valores similares de las tasas de multiplicacion en ambas especies (4,6-4,7 plantulas/explante/4 semanas). Los medios y las condiciones de cultivo senaladas han permitido la multiplicacion rutinaria de las especies por un periodo mayor a los 4 anos. EnglishCocoyam, Xanthosoma sagittifolium (L.) Schott and taro, Colocasia esculenta (L.) Schott are edible herbaceous plants of the Aracea family. They grow in the tropics and their modified stems or corms, are consumed as a staple due to their high starch content. The most common reproduction method for these species is by vegetative cuttings, with low propagation rates and disease dissemination, requiring the procurement of pathogen free plants of both species. The aim of this work was to study optimal conditions for in vitro micropropagation of cocoyam and taro plants. In order to reach the aim, corms of both species were surface sterilized with dish liquid detergent, alcohol and bleach (2,5%). To establish the species in vitro, meristems were cultured in MS-2% sucrose and 0,1 mg l-1 BA - 0,01 mg l-1 NAA. Shoot proliferation was induced in MS-2% sucrose media supplemented with 1 mg l-1 BA, 2 mg l-1 BA, 1 mg l-1 BA–3 mg l1 AG3, 1 mg l-1 BA–0,05 mg l-1 GA3–0,02 mg l-1 NAA y 1 mg l-1 BA–0,5 mg l1 GA3–0,02 mg l-1 NAA. Results showed that the best proliferation rates were obtained with MS-2% sucrose -1 mg l-1 BA. Similar multiplication rates were obtained for both species (4,6-4,7 plants/explant/4 weeks). The methodology presented in this paper allowed the maintenance of cocoyam and taro for more than four years.

Colocasia esculenta

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Acetone fraction from Sechium edule (Jacq.) S.w. edible roots exhibits anti-endothelial dysfunction activity

Journal of Ethnopharmacology (2018)

Celeste Trejo-Moreno Gabriela Castro-Martínez Marisol Méndez-Martínez Enrique Jiménez‐Ferrer José Pedraza‐Chaverrí

Endothelial Dysfunction

Proinflammatory cytokine

10.1016/j.jep.2018.02.036

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Citations (14)