Nilce Maria Martinez-Rossi

Universidade de São Paulo

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i-10 index

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Document Type

Co-Authors

Antônio Rossi

Universidade de São Paulo

122

Pablo Rodrigo Sanches

Universidade de São Paulo

Nalu T. A. Peres

Universidade Federal de Minas Gerais

Tamires A. Bitencourt

St Anna Children's Hospital

Maíra Pompeu Martins

Universidade Federal do Pará

Ana Lúcia Fachin

Universidade de Ribeirão Preto

Gabriela Félix Persinoti

Brazilian Center for Research in Energy and Materials

Diana E. Gras

Instituto de Agrobiotecnología del Litoral

Elza A. S. Lang

Universidade de São Paulo

Mônica Stropa Ferreira-Nozawa

Universidade de Ribeirão Preto

Cooperative Institutions

Universidade de São Paulo

278

Universidade de Ribeirão Preto

128

Universidade Estadual Paulista (Unesp)

Universidade Estadual de Campinas (UNICAMP)

Universidade Federal de São Paulo

Clinics Hospital of Ribeirão Preto

Universidade Brasil

Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo

Universidade Federal do Rio de Janeiro

Instituto Butantan

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Metabarcoding approach to identify bacterial community profiling related to nosocomial infection and bacterial trafficking-routes in hospital environments

Figshare (2023)

Bárbara Gimenes de Castro Bruno Mari Fredi Rafael dos Santos Bezerra Queren Apuque Alcantara Carlos Eduardo Milani Neme

Nosocomial infections (NIs) appear in patients under medical care in the hospital. The surveillance of the bacterial communities employing high-resolution 16S rRNA profiling, known as metabarcoding, represents a reliable method to establish factors that may influence the composition of the bacterial population during NIs. The present study aimed to utilize high-resolution 16S rRNA profiling to identify high bacterial diversity by analyzing 11 inside and 10 outside environments from the General Hospital of Ribeirão Preto Medical School, Brazil. Our results identified a high bacterial diversity, and among these, the most abundant bacterial genera linked to NIs were Cutibacterium, Streptococcus, Staphylococcus, and Corynebacterium. A Acinetobacter was detected in cafeterias, bus stops, and adult and pediatric intensive care units (ICUs). Data suggest an association between transport and alimentation areas proximal to the hospital ICU environment. Interestingly, the correlation and clusterization analysis showed the potential of the external areas to directly influence the ICU pediatric department microbial community, including the outpatient’s clinic, visitor halls, patient reception, and the closest cafeterias. Our results demonstrate that high-resolution 16S rRNA profiling is a robust and reliable tool for bacterial genomic surveillance. In addition, the metabarcoding approach might help elaborate decontamination policies, and consequently reduce NIs.

Profiling (computer programming)

DNA profiling

10.6084/m9.figshare.23931855

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HacA Governs Virulence Traits and Adaptive Stress Responses in Trichophyton rubrum

Frontiers in Microbiology (2020)

Tamires A. Bitencourt Elza A. S. Lang Pablo Rodrigo Sanches Nalu T. A. Peres Vanderci Massaro de Oliveira

The ability of fungi to sense environmental stressors and appropriately respond is linked to secretory system functions. The dermatophyte infection process depends on an orchestrated signaling regulation that triggers the transcription of genes responsible for adherence and penetration of the pathogen into host-tissue. A high secretion system is activated to support the host-pathogen interaction and assures maintenance of the dermatophyte infection. The gateway of secretion machinery is the endoplasmic reticulum (ER), which is the primary site for protein folding and transport. Current studies have shown that ER stress that affects adaptive responses is primarily regulated by UPR and supports fungal pathogenicity; this has been assessed for yeasts and Aspergillus fumigatus, in regard to how these fungi cope with host environmental stressors. Fungal UPR consists of a transmembrane kinase sensor (Ire1/IreA) and a downstream target Hac1/HacA. The active form of Hac is achieved via non-spliceosomal intron removal promoted by endonuclease activity of Ire1/IreA. Here, we assessed features of HacA and its involvement in virulence and susceptibility in Trichophyton rubrum. Our results showed that exposure to antifungals and ER-stressing agents initiated the activation of HacA from T. rubrum. Interestingly, the activation occurs when a 20 nt fragment is removed from part of the exon-2 and part of intron-2, which in turn promotes the arisen of the DNA binding site motif and a dimer interface domain. Further, we found changes in the cell wall and cellular membrane composition in the ΔhacA mutant as well as an increase in susceptibility toward azole and cell wall disturbing agents. Moreover, the ΔhacA mutant presented significant defects in important virulence traits like thermotolerance and growth on keratin substrates. For instance, the development of the ΔhacA mutant was impaired in co-culture with keratinocytes or human nail fragments. Changes in the pro-inflammatory cytokine release were verified for the ΔhacA mutant during the co-culture assay, which might be related to differences in pathogen-associated molecular patterns (PAMPs) in the cell wall. Together, these results suggested that HacA is an integral part of T. rubrum physiology and virulence, implying that it is an important molecular target for antidermatophytic therapy.

10.3389/fmicb.2020.00193

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Citations (18)

Cloning of a gene pacC homologue from Neurospora crassa

Programa e Resumos (1999)

Shunsuke Nozawa W. Macheroni M. S. Ferreira Nilce Maria Martinez-Rossi Antônio Rossi

Cloning (programming)

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Análise de genes diferencialmente expressos no dermatófito Trichophyton rubrum em condição de estresse com cloranfenicol utilizado DDRT-PCR

Programa e Resumos (2003)

Henrique C.S. Silveira Sérgio Ricardo Nozawa Nilce Maria Martinez-Rossi

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The gene that determines resistance to tioconazole and to acridine derivatives in Aspergillus nidulans may have a corresponding gene in Trichophyton rubrum.

Mycopathologia (1998)

Marcelo Pereira Ana Lúcia Fachin Nilce Maria Martinez-Rossi

Acriflavine

Aspergillus nidulans

Ethidium bromide

Acridine

10.1023/a:1006919009621

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Citations (9)

Comparative metabolism of cellulose, sophorose and glucose in Trichoderma reeseiusing high-throughput genomic and proteomic analyses

Biotechnology for Biofuels (2014)

Lílian dos Santos Castro Wellington Ramos Pedersoli Amanda Cristina Campos Antoniêto Andrei Stecca Steindorff Rafael Silva‐Rocha

The filamentous fungus Trichoderma reesei is a major producer of lignocellulolytic enzymes utilized by bioethanol industries. However, to achieve low cost second generation bioethanol production on an industrial scale an efficient mix of hydrolytic enzymes is required for the deconstruction of plant biomass. In this study, we investigated the molecular basis for lignocellulose-degrading enzyme production T. reesei during growth in cellulose, sophorose, and glucose. We examined and compared the transcriptome and differential secretome (2D-DIGE) of T. reesei grown in cellulose, sophorose, or glucose as the sole carbon sources. By applying a stringent cut-off threshold 2,060 genes were identified as being differentially expressed in at least one of the respective carbon source comparisons. Hierarchical clustering of the differentially expressed genes identified three possible regulons, representing 123 genes controlled by cellulose, 154 genes controlled by sophorose and 402 genes controlled by glucose. Gene regulatory network analyses of the 692 genes differentially expressed between cellulose and sophorose, identified only 75 and 107 genes as being specific to growth in sophorose and cellulose, respectively. 2D-DIGE analyses identified 30 proteins exclusive to sophorose and 37 exclusive to cellulose. A correlation of 70.17% was obtained between transcription and secreted protein profiles. Our data revealed new players in cellulose degradation such as accessory proteins with non-catalytic functions secreted in different carbon sources, transporters, transcription factors, and CAZymes, that specifically respond in response to either cellulose or sophorose.

10.1186/1754-6834-7-41

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Citations (140)

Effect of sub-MICs of antimycotics on expression of intracellular esterase ofTrichophyton rubrum

Medical Mycology (2001)

AL Fachin Eucléia Primo Betioli Contel Nilce Maria Martinez-Rossi

The electrophoretic pattern of the intracellular esterase of the dermatophyte Trichophyton rubrum was altered when this fungus was grown in the presence of subinhibitory concentrations of the antimycotics tioconazole or griseofulvin. All strains (original isolate and antimycotic resistant mutants) presented five clearly visible bands when cultivated on medium containing below-minimum inhibitory concentrations (sub-MICs) of tioconazole or griseofulvin, and only two clearly visible bands when cultivated in medium without antimycotics. No extra bands were detected in the electrophoretic patterns of the extracellular esterase of these fungi (mutants or the original isolate) when cultivated with or without tioconazole or griseofulvin (sub-MIC values). These results suggest that additional forms of esterase are produced inside the cell and may be a nonspecific response to cellular stress, or may participate in cellular detoxification processes in the presence of these antimycotics.

Griseofulvin

Esterase

10.1080/mmy.39.1.129.133

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Citations (21)

A regulação pelo pH no fungo Neurospora crassa apresenta similaridades àquela exercida pelo fungo Aspergillus nidulans

K. C. Nogueira André Justino Shunsuke Nozawa Antônio Rossi Nilce Maria Martinez-Rossi

Aspergillus nidulans

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Evolution of hepatitis C virus infection under host factor influence in an ethnically complex population

Liver International (2007)

Jeny R. Cursino‐Santos Eduardo Antônio Donadi Ana L.C. Martinelli Paulo Louzada‐Júnior Nilce Maria Martinez-Rossi

Abstract Background: The ethnic influence makes it difficult to reach a consensual definition of host‐dependent genetic factors controlling the hepatitis C virus (HCV) disease course. Aims: To investigate, in an ethnically complex Brazilian population, whether human leucocyte antigen (HLA) molecules are associated with susceptibility to HCV infection, self‐limiting viral clearance and predisposition to chronic disease. Methods: One hundred and four HCV‐antibody‐positive patients (stratified into groups with spontaneous viral clearance and chronic HCV infection) and 166 healthy controls were submitted to HLA genotyping. Results: Two strong associations were observed between the susceptibility to HCV infection and DRB3 [odds ratio (OR), 4.03; 95% confidence interval (CI), 2.40–6.77; P c =0.0000041] and DQB1 * 02 (OR, 1.72; 95% CI, 1.05–2.84; P =0.041), and between the spontaneous viral clearance and DRB1 * 01 (OR, 4.59; 95% CI, 1.70–12.41; P =0.003) and DQB1 * 03 (OR, 2.83; 95% CI, 1.14–7.02; P =0.029). No evidence was observed regarding the epidemiology or viral genotype influence on the disease course. Conclusion: We could confirm with a highly admixed population the association of viral clearance with two allele groups ( DRB1 * 01 and DQB1 * 03 ) previously reported in homogeneous populations. The identification of DRB1 * 01 and DQB1 * 03 involved with self‐limiting hepatitis in different ethnic groups is a very important finding that will contribute to the current knowledge about HCV–host interaction and the development of therapeutic vaccines.

Hepatitis C

Liver disease

10.1111/j.1478-3231.2007.01600.x

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Citations (12)

A splice variant of theNeurospora crassa hex‐1transcript, which encodes the major protein of the Woronin body, is modulated by extracellular phosphate and pH changes

FEBS Letters (2008)

Juliana Leal Fábio M. Squina Janaína S. Freitas Emiliana M. Silva Carlos J. Ono

The Woronin body, a septal pore‐associated organelle specific to filamentous ascomycetes, is crucial for preventing cytoplasmic bleeding after hyphal injury. In this study, we show that T1 hex‐1 transcript and a variant splicing T2 hex‐1 transcript are up‐regulated at alkaline pH. We also show that both hex‐1 transcripts are overexpressed in the preg c , nuc‐1 RIP , and pacC ko mutant strains of Neurospora crassa grown under conditions of phosphate shortage at alkaline pH, suggesting that hex‐1 transcription may be coregulated by these genes. In addition, we present evidence that N. crassa PacC also has metabolic functions at acidic pH.

Crassa

10.1016/j.febslet.2008.11.050

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Citations (35)