Aim:The given RP-HPLC technique was found to be clear-cut, specific, exact, and economical for the estimation of Velpatasvir and Sofosbuvir in bulk and tablet dosage form.Methodology: Stationary phase Hypersil C18 column (4.6×150, 5µm) was utilized as chromatographic conditions.Water: Acetonitrile in the ratio of 30:70 was used as a fluid phase.The flow rate was maintained at 1.0ml/min Results: The wavelength of detection was 230 nm.Velpatasvir linearity was found to be 10-50 ppm and Sofosbuvir linearity was found to be 20-100 ppm with r 2 =0.999.Assessment of the formulated market was carried out by using the above method and the percentage of Velpatasvir and Sofosbuvir was found to be 99.6 % and 99.8% respectively. Conclusion:Hence the method can be successfully applied for routine analysis of Velpatasvir and Sofosbuvir in bulk and tablet formulation.
Tolvaptan in bulk and formulation in spiking human plasma were estimated using a brand-new, straightforward, speedy, precise, and accurate bioanalytical method that was developed and validated using UV-visible spectrophotometer. The acetonitrile-based solvent chosen for the tolvaptan UV study was scanned over the UV spectrum from 200 to 400nm using a solution containing 10g/ml. At 267nm, Tolvaptan exhibits its greatest absorption. The accuracy investigations were conducted at three distinct levels, i.e., 80%, 100%, and 120%, and recovery was found to be in the range of 99.4%. The tolvaptan showed linearity over the range of 5-160g/mL with correlation coefficientnt (r2) of 0.999. The thresholds for detection and quantification were 0.471 and 1.435 g/ml, respectively. In accordance with ICH requirements, all the parameters were validated.
The estimation of sitagliptin in tablet and bulk dose forms has been created using a straight forward UV approach.In all experiments, water was utilized as the solvent.It was discovered that water had an absorption maximum of 267 nm.A linearity range of between 10-300 µg/ml was obtained after performing all the validation parameters.%Recovery of the proposed method was found to be between 97.12 and 99.46, and its %RSD was discovered to be within acceptable bounds, or less than 2. The thresholds for detection and quantitation were determined to be 3.397 and 10.295 µg/ml, respectively.Stress studies were also performed for the drug substance.In addition to checking the absorbance of the deteriorated product, stress investigations such as acid and alkali hydrolysis, oxidation, photolytic, and thermal were conducted.
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