Sandra Kim Tiam

Centre National de la Recherche Scientifique

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Soizic Morin

Centre d'Études Scientifiques et Techniques d'Aquitaine

Nicolás Mazzella

Centre d'Études Scientifiques et Techniques d'Aquitaine

Patrice Gonzalez

Université de Bordeaux

Stéphane Pesce

Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

Benjamin Marie

Molécules de Communication et Adaptation des Micro-organismes

Cécile Bernard

Muséum national d'Histoire naturelle

A. Feurtet Mazel

Environnements et Paléoenvironnements Océaniques et Continentaux

Claude Fortin

Institut National de la Recherche Scientifique

Isabelle Lavoie

Institut National de la Recherche Scientifique

François Delmas

Centre d'Études Scientifiques et Techniques d'Aquitaine

Cooperative Institutions

Centre National de la Recherche Scientifique

Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

Stress Environnementaux et Biosurveillance des Milieux Aquatiques

Muséum national d'Histoire naturelle

Environnements et Paléoenvironnements Océaniques et Continentaux

Molécules de Communication et Adaptation des Micro-organismes

Sorbonne Université

Centre d'Études Scientifiques et Techniques d'Aquitaine

Université de Bordeaux

Commissariat à l'Énergie Atomique et aux Énergies Alternatives

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Diatom Deformities and Tolerance to Cadmium Contamination in Four Species

Environments (2019)

Sandra Kim Tiam Isabelle Lavoie Fengjie Liu Paul B. Hamilton Claude Fortin

The relative tolerance of four diatoms (Nitzschia palea, Pinnularia mesolepta, Mayamaea atomus, and Gomphonema truncatum) to Cd was evaluated, including their proneness to deformities, and the severity of the abnormalities in relation to Cd concentration. The indirect effect of Cd on photosynthetic capacities was assessed during a short time exposure experiment using a dose-response approach to evaluate the relative tolerance of the four diatom species. The EC25 were 9 (3, 23), 606 (348, 926), 1179 (1015, 1349) and 2394 (1890, 2896) µg/L for P. mesolepta, G. truncatum, N. palea, and M. atomus respectively. P. mesolepta was by far the most Cd sensitive species while M. atomus was the most tolerant. In addition, diatoms were exposed to a single concentration of Cd comparable to a heavily contaminated environment for a longer duration to evaluate the effect of Cd on growth kinetics and the deformities induced. N. palea, P. mesolepta, and M. atomus were able to grow when cultivated with Cd while G. truncatum was not. Cadmium strongly affected the effective quantum yield in G. truncatum (4.8 ± 5.9% of the control) and P. mesolepta cultures (29.2 ± 6.9% of the control). The effects were moderate for N. palea (88.3 ± 0.7% of the control) and no impact was observed for M. atomus. The results from the two approaches were in accordance since they identified N. palea and M. atomus as the two most tolerant species to Cd, while P. mesolepta and G. truncatum were the most sensitive. The microscopy analyses revealed that P. mesolepta was more impacted by Cd than N. palea and M. atomus considering both the quantity of abnormal cells and the severity of the deformities. Overall, this research shows that not all deformities can be considered equal for a water quality bio-assessment. The work highlights a need to take into account metal-tolerance/sensitivity of the species and the severity of the deformities.

10.3390/environments6090102

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Development of q-PCR approaches to assess water quality: Effects of direct cadmium exposure on gene expression of the diatom Eolimna minima

HAL (Le Centre pour la Communication Scientifique Directe) (2011)

Sandra Kim Tiam A. Feurtet Mazel François Delmas Nicolás Mazzella Soizic Morin

Maxima and minima

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Genomic Insights of Alnus-Infective Frankia Strains Reveal Unique Genetic Features and New Evidence on Their Host-Restricted Lifestyle

Genes (2023)

Sandra Kim Tiam Hasna Boubakri Lorine Béthencourt Danis Abrouk Pascale Fournier

The present study aimed to use comparative genomics to explore the relationships between Frankia and actinorhizal plants using a data set made of 33 Frankia genomes. The determinants of host specificity were first explored for "Alnus-infective strains" (i.e., Frankia strains belonging to Cluster Ia). Several genes were specifically found in these strains, including an agmatine deiminase which could possibly be involved in various functions as access to nitrogen sources, nodule organogenesis or plant defense. Within "Alnus-infective strains", Sp+ Frankia genomes were compared to Sp- genomes in order to elucidate the narrower host specificity of Sp+ strains (i.e., Sp+ strains being capable of in planta sporulation, unlike Sp- strains). A total of 88 protein families were lost in the Sp+ genomes. The lost genes were related to saprophytic life (transcriptional factors, transmembrane and secreted proteins), reinforcing the proposed status of Sp+ as obligatory symbiont. The Sp+ genomes were also characterized by a loss of genetic and functional paralogs, highlighting a reduction in functional redundancy (e.g., hup genes) or a possible loss of function related to a saprophytic lifestyle (e.g., genes involved in gas vesicle formation or recycling of nutrients).

Frankia

10.3390/genes14020530

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Effects of environmental realistic mixture of pesticides on natural biofilms communities with different exposure history

HAL (Le Centre pour la Communication Scientifique Directe) (2013)

Sandra Kim Tiam Stéphane Pesce A. Feurtet Mazel Nicolás Mazzella Soizic Morin

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Light stress in green and red Planktothrix strains: The orange carotenoid protein and its related photoprotective mechanism

Biochimica et Biophysica Acta (BBA) - Bioenergetics (2019)

Chakib Djédiat Kathleen Feilke Arthur Brochard Lucie Caramelle Sandra Kim Tiam

10.1016/j.bbabio.2019.06.009

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The success of the bloom-forming cyanobacteria Planktothrix: Genotypes variability supports variable responses to light and temperature stress

Harmful Algae (2022)

Sandra Kim Tiam Katia Comte Caroline Dalle Marine Delagrange Chakib Djédiat

Cyanobacterial blooms can modify the dynamic of aquatic ecosystems and have harmful consequences for human activities. Moreover, cyanobacteria can produce a variety of cyanotoxins, including microcystins, but little is known about the role of environmental factors on the prevalence of microcystin producers in the cyanobacterial bloom dynamics. This study aimed to better understand the success of Planktothrix in various environments by unveiling the variety of strategies governing cell responses to sudden changes in light intensity and temperature. The cellular responses (photosynthesis, photoprotection, heat shock response and metabolites synthesis) of four Planktothrix strains to high-light or high-temperature were studied, focusing on how distinct ecotypes (red- or green-pigmented) and microcystin production capability affect cyanobacteria's ability to cope with such abiotic stimuli. Our results showed that high-light and high-temperature impact different cellular processes and that Planktothrix responses are heterogeneous, specific to each strain and thus, to genotype. The ability of cyanobacteria to cope with sudden increase in light intensity and temperature was not related to red- or green-pigmented ecotype or microcystin production capability. According to our results, microcystin producers do not cope better to high-light or high-temperature and microcystin content does not increase in response to such stresses.

Ecotype

Light intensity

Bloom

10.1016/j.hal.2022.102285

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qPCR detection versus microscopy observations for assessing presence–absence of Didymosphenia geminata in Quebec rivers (Canada)

Water Quality Research Journal (2015)

Sandra Kim Tiam Vincent Laderrière Carole‐Anne Gillis Claude Fortin Isabelle Lavoie

Microscopic versus qPCR (quantitative polymerase chain reaction) analyses were compared for the detection of Didymosphenia geminata in biofilms and water filtrates from seven Gaspésie rivers (Canada). For the qPCR approach, two DNA extraction kits (QIAamp DNA Micro Kit, Qiagen and PowerSoil DNA Isolation Kit, Mo Bio Laboratories) and two pairs of primers were considered. The pair of primers D602F/D753Rext did not amplify D. geminata DNA whereas the pair of primers D602F/D753R was specific for D. geminata. Presence-absence diagnosis based on qPCR and microscopic analyses were consistent: D. geminata was detected in six of the seven rivers, both in the biofilm and filtrate samples. However, technical replications were needed at certain sites to observe the presence of D. geminata cells by microscopy. This underscores the necessity of replicate analyses, which is cost-effective to achieve when using qPCR due to the capacity to process tens of samples in a single PCR run in the context of a large scale assessment.

Replicate

Environmental DNA

10.2166/wqrj.2017.030

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Citations (1)

Effects of cadmium on the gene expression of the species Eolimna minima

HAL (Le Centre pour la Communication Scientifique Directe) (2012)

Sandra Kim Tiam A. Feurtet Mazel François Delmas Nicolás Mazzella Soizic Morin

Maxima and minima

Expression (computer science)

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Dynamics of the Metabolome of Aliinostoc sp. PMC 882.14 in Response to Light and Temperature Variations

Metabolites (2021)

Damien Le Moigne Justine Demay Anita Reinhardt Cécile Bernard Sandra Kim Tiam

Cyanobacteria are microorganisms able to adapt to a wide variety of environmental conditions and abiotic stresses. They produce a large number of metabolites that can participate in the dynamic adaptation of cyanobacteria to a range of different light, temperature, and nutrient conditions. Studying the metabolite profile is one way to understand how the physiological status of cells is related to their adaptive response. In this study, we sought to understand how the diversity and dynamics of the whole metabolome depended on the growth phase and various abiotic factors such as light intensity and temperature. The cyanobacterium, Aliinostoc sp. PMC 882.14, was selected for its large number of biosynthetic gene clusters. One group of cells was grown under normal conditions as a control, while other groups were grown under higher light or temperature. Metabolomes were analyzed by mass spectrometry (qTOF-MS/MS) combined with untargeted analysis to investigate metabolite dynamics, and significant variation was found between exponential and stationary phases, regardless of culture conditions. In the higher light group, the synthesis of several metabolites, including shinorine, was induced while other metabolites, such as microviridins, were synthesized under higher temperature conditions. Among highly regulated metabolites, we observed the presence of mycosporine-like amino acids (MAAs) and variants of somamides, microginins, and microviridins. This study demonstrated the importance of considering the physiological state of cyanobacteria for comparative global metabolomics and studies of the regulatory processes involved in production of specific metabolites. Our results also open up new perspectives on the use of organisms such as cyanobacteria for the targeted production of bioactive metabolites.

Metabolome

Dynamics

10.3390/metabo11110745

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Citations (12)

Genetic and physiological responses of three freshwater diatoms to realistic diuron exposures

Environmental Science and Pollution Research (2014)

Sophie Moisset Sandra Kim Tiam Agnès Feurtet‐Mazel Soizic Morin F. Delmas

10.1007/s11356-014-3523-2

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Citations (39)