Hekmat Osman Abdel-Aziz

Sohag University

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Yoshihiro Murai

Tohoku Medical and Pharmaceutical University

Koichi Tsuneyama

Tokushima University

Yasuo Takano

Kochi Rehabilitation Institute

Kazuhiro Nomoto

Kouseiren Takaoka Hospital

Hiroyuki Takahashi

Kanagawa Cancer Center

Hoda Elsayed

Sohag University

Ghada Mohammed Ahmed

Sohag University

Yoshiaki Tabuchi

University of Toyama

Kouichiro Shinoda

University of Toyama

Masashi Kobayashi

Obayashi (Japan)

Cooperative Institutions

University of Toyama

Tohoku Medical and Pharmaceutical University

Sohag University

Assiut University

Tohoku University

Kanagawa Prefectural Hospital Organization

South Valley University

Al-Azhar University

Kanagawa Cancer Center

University of California, Davis

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Targeted disruption of the galectin-3 gene results in decreased susceptibility to NNK-induced lung tumorigenesis: an oligonucleotide microarray study

Journal of Cancer Research and Clinical Oncology (2008)

Hekmat Osman Abdel-Aziz Yoshihiro Murai Ichiro Takasaki Yoshiaki Tabuchi Hua-chuan Zheng

Galectin-3

10.1007/s00432-007-0345-3

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Citations (34)

Ultrastructural study of the effect of carbon tetrachloride on the pars distalis of the anterior pituitary gland of mice and the possible protective role of ginger

The Egyptian Journal of Histology (2012)

Hekmat Osman Abdel-Aziz Nesreen G.A. El Haliem

Background Free radicals and oxidative stress can be induced by a variety of factors, such as carbon tetrachloride (CCl4). Zingiber officinale Roscoe (Ginger) has antioxidant, antitumoral, and antihepatotoxic activities. Aim of the work The present study was carried out to assess the possible role of ginger on CCl4-induced toxic effects on the pars distalis. Materials and methods Thirty adult male mice were used and divided into three groups (10 animals each). The first group (group I) was the control group. The second group (group II) received CCl4 dissolved in an equal amount of paraffin oil at a dose of 0.2 ml/kg body weight by subcutaneous injection twice weekly for 4 weeks. The third group (group III) received CCl4 in the same previous dose by subcutaneous injection concomitant with ginger at a dose of 1 ml of final aqueous extract of ginger (24 mg/ml) once a day by intragastric intubation for 4 weeks. The pituitary gland was dissected out and processed for examination by light and transmission electron microscopy. Results In CCl4-treated mice, most cells of the pars distalis showed many histological changes. Somatotrophs, thyrotrophs, and corticotrophs exhibited irregular nuclei, numerous vacuoles, and disrupted organelles. Binucleated somatotrophs were observed. The gonadotroph cells had dilated rough endoplasmic reticulum and a few secretory granules. These cellular changes were found to be diminished by ginger. Conclusion It was concluded that exposure to CCl4 induced a toxic effect on the pars distalis. Concomitant administration of ginger decreased the toxicity of CCl4.

Thyrotropic cell

CCL4

Zingiber officinale

Subcutaneous injection

10.1097/01.ehx.0000418458.34353.62

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Interleukin-10 inhibits RANKL-mediated expression of NFATc1 in part via suppression of c-Fos and c-Jun in RAW264.7 cells and mouse bone marrow cells

Bone (2007)

Saad Gad-Kamel Mohamed Eiji Sugiyama Kouichiro Shinoda Hirofumi Taki Hiroyuki Hounoki

c-jun

10.1016/j.bone.2007.05.016

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Citations (111)

Periostin expression and characters of human adipose tissue-derived mesenchymal stromal cells were aberrantly affected by in vitro cultivation

Stem Cell Investigation (2019)

Heba M. Saad Eldien Hekmat Osman Abdel-Aziz Douaa Sayed W. Mubarak Hemmat H. G. Hareedy

Human adipose tissue-derived mesenchymal stromal cells (AD-MSCs) have been under focus in regenerative medicine since their discovery as a suitable source of MSCs. AD-MSCs are heterogeneous cells and exhibit variations in population doubling time, morphology and proliferative capacity. This study investigated if human AD-MSCs are developing, during in vitro long-term cultivation, in an unwanted or aberrant way.This study monitored AD-MSCs during their in vitro culture till the tenth passage investigating proliferation kinetics, DNA index and surface markers expression. Also, periostin gene expression was examined.The proliferation capacity and colony forming unit were decreased after passage 6 and the population doubling time was increased. Flow cytometric analysis revealed that newly cultivated population strongly expressed MSCs markers, furthermore, reduction of CD105 expression appeared in passage 5 onwards, the later was associated with significant increase in expression of CD34 (a hematopoietic cell marker). Also, reduction of CD73 and CD90 expression was observed from passage 8. Furthermore, during the first six passages, periostin expression was significantly unchanged, with significant upregulation in late passages.Long-term cultivation of human AD-MSCs changed their characters in an aberrant way and the first four passages might be the most appropriate passages for therapy. More investigation and understanding of these variations are needed to help in standardizing the expansion of MSCs-based therapies.

Periostin

CD90

10.21037/sci.2019.08.09

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Citations (6)

Analysis using high-density oligonucleotide microarrays on genetic alteration of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) induced lung tumorigenesis in galectin-3 knockout and wild mice

Hekmat Osman Abdel-Aziz

Knockout mouse

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Activation of peroxisome proliferator-activated receptor γ inhibits TNF-α-mediated osteoclast differentiation in human peripheral monocytes in part via suppression of monocyte chemoattractant protein-1 expression

Bone (2008)

Hiroyuki Hounoki Eiji Sugiyama Saad Gad-Kamel Mohamed Kouichiro Shinoda Hirofumi Taki

Monocyte

10.1016/j.bone.2007.11.016

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Citations (71)

High JC virus load in tongue carcinomas may be a risk factor for tongue tumorigenesis

Virchows Archiv (2008)

Tomohiko Kutsuna Hua‐chuan Zheng Hekmat Osman Abdel-Aziz Yoshihiro Murai Koichi Tsuneyama

The John Cunningham virus (JCV) asymptomatically infects a large proportion (approximately 90%) of the population worldwide but may be activated in immunodeficient patients, resulting in progressive multifocal leukoencephalopathy. Recent reports demonstrated its oncogenic role in malignancies. In this paper, the presence of JCV-targeting T antigen was investigated in tongue carcinoma (TC, n = 39), dysplastic tongue epithelium (DTE, n = 15) and glossitis (n = 15) using real-time polymerase chain reaction (PCR) and in situ PCR and immunohistochemistry, and JCV copies were analyzed with the clinicopathological parameters of TCs. The results demonstrated that glossitis and DTEs had significantly lower copies of JCV (410.5 +/- 44.3 and 658.3 +/- 53.3 copies/mug DNA respectively) than TCs (981.5 +/- 14.0, p < 0.05). When they were divided into three groups with 0-200 copies/mug DNA (low), 201-1,000 (moderate) and more than 1001 (high), TCs showed 3 (7.6%) in the low group, 21 (53.8%) in the moderate group and 15 (38.4%) in the high group and glossitis showed 11 (73.3%) in the low group, 0 (0%) in the moderate group and 4 (26.6%) in the high group. The DTEs occupied an intermediate position between them (p < 0.001). In situ PCR demonstrated that the nuclei of TC and DTE cells are sporadically T-antigen positive but not in nasal turbinate epithelial cells. Immunohistochemistry for T-antigen protein revealed four positive cases only in TCs. The existence of JCV T-antigen DNA was not associated with the clinicopathological variables of TCs. In conclusion, the presence of JCV may be a risk factor of tongue carcinogenesis.

JC Virus

Glossitis

Tongue Neoplasm

10.1007/s00428-007-0534-0

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Citations (30)

The possible ameliorative effect of Echinacea, ginger, and their combination on experimentally induced diabetic nephropathy in a rat model: Histological and immunohistochemical study

Journal of Microscopy and Ultrastructure (2023)

Hoda Elsayed Hekmat Osman Abdel-Aziz Ghada Mohammed Ahmed Mohamed Arafa Adly Sherine Ahmed Mohammed

Rat model

10.4103/jmau.jmau_62_22

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Citations (1)

Effect of Nicotine on STAT1 Pathway and Oxidative Stress in Rat Lungs

Reports of Biochemistry and Molecular Biology (2021)

Aida A. Mahmoud Hekmat Osman Abdel-Aziz Mohamed M. Elbadr Hala M. ElBadre

Tobacco use is responsible for millions of preventable deaths due to cancer. Nicotine, an alkaloid chemical found in tobacco was proved to cause chronic inflammation and oxidative stress. The transcription factor STAT1 induces the expression of many proinflammatory genes and has been suggested to be a target for anti-inflammatory therapeutics. The following study investigated the effect of Nicotine on STAT1 pathway and oxidative stress in rat lung tissue.Thirty rats were divided into 3 groups; group I considered as control, group II; its rats were daily injected with Nicotine at a dose of 0.4 mg/100 gm body for 8 successive weeks and group III; its rats were daily injected with Nicotine as group II, but the injection was stopped for another 4 weeks. STAT1α protein was assessed by immunohistochemistry, COX-2 and iNOS genes expression were evaluated by real time PCR and thiobarbituric acid reactive substances (TBARS) and total thiols were measured using spectrophotometric methods in the lung tissues of the rats.The results of the study revealed that group II rats had the highest expression of STAT1α protein and COX-2 and iNOS genes and oxidative stress in their lung tissues. Nicotine cessation for 4 weeks caused a marked reduction in the expression of STAT1α protein, COX-2 and iNOS genes and oxidative stress.Induction of STAT1 pathway and the increase in oxidative stress may be the mechanisms through which Nicotine may induce its harmful effects.

TBARS

STAT1

Proinflammatory cytokine

10.52547/rbmb.10.3.429

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Citations (1)

The Possible Protective Role of Echinacea and Ginger and both of them on the Lung of Diabetic Male Rats: Histological and Immunohistochemical Study

The Egyptian Journal of Histology (2021)

Ghada H. Ahmed Hekmat Osman Abdel-Aziz Hoda Elsayed Mohammed Adly

Introduction: Diabetes is a systemic disease characterized by a state of hyperglycemia. Several changes in the respiratory functions had been detected in diabetic patients.Aim of the Work: The current study estimated the efficiency of two herbal extract (ginger and echinacea) on the changes in the lung of diabetic animal model.Materials and Methods: 50 adult male albino rats were used. They were divided into five groups. Control group (group I) received regular diet. Group II: diabetic group (diabetes induced by a single intraperitoneal injection of streptozotocin 60 mg/kg). Group III: diabetic rats treated with 100mg/kg echinacea orally for 30 days. Group IV: diabetic rats treated with 400mg/kg ginger orally for 30 days. Group V: diabetic rats treated with both 100mg/kg echinacea and 400mg/kg ginger orally for 30 days. Paraffin blocks were prepared for histological and immunohistochemical examinations. Morphometric study and statistical analysis were done for the thickness of the interalveolar septum, the area percentage of collagen fibers and number of positive caspase -3 cells and CD68 positive macrophages. Ultrathin sections were prepared for electron microscopic examination.Results: Diabetes caused a significant increase in the thickness of the interalveolar septa, the area percentage of collagen fibers, the number of caspase-3 positive cells as well as the number of CD68 positive alveolar macrophages compared to the control group. Ultrastructurally, pneumocyte type II appeared with denser nucleus, numerous vacuoles and disorganized lamellar bodies.Conclusion: Groups treated with echinacea or ginger or both showed a significant decrease in the thickness of the interalveolar septa, the area percentage of collagen fibers, the number of caspase-3 positive cells as well as the number of CD68 positive alveolar macrophages compared to the diabetic group. These groups also showed that pneumocyte type II restored their normal appearance.

Echinacea

10.21608/ejh.2021.71195.1457

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Citations (1)