Juan Du

Capital Medical University

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Zengqiang Zhang

Northwest A&F University

Ronghua Li

Northwest A&F University

Di Guo

Chinese Academy of Medical Sciences & Peking Union Medical College

Jianhua Xiong

Jiangxi Agricultural University

Jingwen Chen

Dalian University of Technology

Ran Xiao

Southwest University

Amjad Ali

China University of Geosciences

Hongxia Zhao

Dalian University of Technology

Aibing Chen

Hebei University of Science and Technology

Huaijun Xie

Dalian University of Technology

Cooperative Institutions

Chinese Academy of Sciences

173

University of Chinese Academy of Sciences

Xiaomi (China)

Ross School

Zhejiang University

Sichuan University

Jiangxi Agricultural University

Northwest A&F University

Chinese Academy of Medical Sciences & Peking Union Medical College

Ministry of Agriculture and Rural Affairs

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Phosphine-Catalyzed Tandem Annulation of Allenylic Alcohols with 1,1-Dicyanoalkenes

Organic Letters (2023)

Zeqing Duan Min Liu Bing Zheng Yi Tang Juan Du

The phosphine-catalyzed tandem annulation of allenylic alcohols with 1,1-dicyanoalkenes has been developed, giving various bicyclic tetrahydrocyclopentafuran derivatives in 40-89% yields with moderate to excellent diastereoselectivities. The fused ring was furnished through a sequential (3 + 2) annulation/nucleophilic addition reaction. The unusual nucleophilic addition of an alkoxide ion to a cyano group led to a formation of tetrahydrofuran ring having an imino substituent.

Annulation

Tandem

Tetrahydrofuran

Alkoxide

Nucleophilic Addition

10.1021/acs.orglett.3c01066

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Citations (13)

Elicitation of Lonicera japonica Thunb suspension cell for enhancement of secondary metabolites and antioxidant activity

Industrial Crops and Products (2020)

Lianduo Du Dongmei Li Jianjun Zhang Juan Du Qiushui Luo

ABTS

Phytochemical

10.1016/j.indcrop.2020.112877

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Citations (26)

HPLC-MS法による少腹逐Yu丸及び原料薬に不法添加された7種の黄色色素を検出した。【JST・京大機械翻訳】

Zhongcaoyao (2016)

Yunlan Lian Juan Du Qiao Yufeng

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Aptamer-mediated Colorimetric Detection of Florfenicol Utilizing Peroxidase-like Activity of Gold Nanoparticles (利用金纳米颗粒的过氧化物酶样活性的磷酰胺介导的氟苯尼考比色检测)

Danfeng Zhang Yue-Yue Feng Ting-Rui Fu Zhou Zhi-e Xiang Geng

To rapidly detect florfenicol (FF) in eggs, a method based on the aptamer-enhanced peroxidase-like activity of gold nanoparticles (AuNPs) was established. TMB-H2O2 was used as the substrates and the aptamers modified on AuNPs were used to promote the generation of hydroxyl radicals through electrostatic attraction between TMB and AuNPs, causing the peroxidase-like activity of AuNPs enhanced. With the addition of FF which aptamers priority combined to, the active sites of AuNPs were covered by aptamers with FF leading to the peroxidase-like activity reduced. Based on this, a method for detecting FF was developed, and the method had good specificity and anti-interference ability. The detection of FF in real samples was validated, and the recovery rate was from 89.22% to 110.23% with an RSD of 6.79%. The use of aptamers can enhance the peroxidase-like activity of AuNPs and quantitatively detect FF, which provides a novel strategy for the simple colorimetric detection of FF in eggs.

Aptamer

Florfenicol

10.2139/ssrn.4458381

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Effect of pyrolysis temperature on chemical form, behavior and environmental risk of Zn, Pb and Cd in biochar produced from phytoremediation residue

Bioresource Technology (2017)

Hui Huang Wenlin Yao Ronghua Li Amjad Ali Juan Du

Ecotoxicity

Residue (chemistry)

10.1016/j.biortech.2017.10.020

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Citations (158)

Evaluation of antibacterial and antifungal properties of Lonicera japonica Thunb . Leaves mediated silver nanoparticles and mechanism investigation

Journal of Food Processing and Preservation (2022)

Dan‐Feng Zhang Zhou Zhi-e Juan Du Xiaoning Liao Gao‐Ding Xu

Lonicera japonica Thunb. leaves were used to biosynthesize silver nanoparticles (AgNPs), while its antibacterial and antifungal properties were investigated. Reaction conditions of AgNPs which characterized were optimized. The maximum absorption wavelength of the AgNPs was located about 400 nm, with a spherical shape and a particle size of 14.87±4.07 nm. Produced AgNPs showed effective antibacterial activities against tested bacterial strains. Minimal inhibitory concentrations and minimum bactericidal concentrations of the AgNPs were 0.25 and 0.5 mg/mL for Escherichia coli (E. coli), and 0.5 and 1.0 mg/mL for Staphylococcus aureus, respectively. According to the mechanism study, AgNPs generated morphological changes in E. coli and disrupted membrane integrity. Furthermore, AgNPs which showed effective dose-dependent inhibitory effects against Penicillium citrinum could suppress the growth of fungal mycelium on citrus peels. Conclusively, AgNPs generated by this facile and green method showed effective antimicrobial activity, which is promising in food processing and preservation.

Penicillium citrinum

Silver nanoparticle

10.1111/jfpp.16896

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Citations (4)

Influent salinity affects substrate selection in surface flow constructed wetlands

Environmental Science and Pollution Research (2021)

Shenyan Zhou Ran Zhao Qiming Li Juan Du Chen Chen

10.1007/s11356-021-15036-5

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Citations (3)

Author Index

Journal of Biological Chemistry (2015)

Natalia Abuladze A. Silvia L Mario Krishna Arudra Isabelle Aubry

10.1016/s0021-9258(19)46803-8

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RH3 enhances antiviral defense by facilitating small RNA loading into Argonaute 2 at endoplasmic reticulum–chloroplast membrane contact sites

Nature Communications (2025)

Juan Huang Juan Du Yan Liu Lu Lu Yanzhuo Xu

While RNA silencing is crucial for plant resistance against viruses, the cellular connections between RNA silencing and antiviral responses in plants remain poorly understood. In this study, we aim to investigate this relationship by examining the subcellular localization of small RNA loading and viral replication in Arabidopsis. Our findings reveal that Argonaute 2 (AGO2), a key component of RNA silencing, loads small RNAs at the endoplasmic reticulum (ER)–chloroplast membrane contact sites (MCSs). We identify a chloroplast-localized protein, RNA helicase 3 (RH3), which interacts with AGO2 and facilitates the loading of small RNAs into AGO2 at these MCSs. Furthermore, we discover that MCSs serve as replication sites for certain plant viruses. RH3 also promotes the loading of viral-derived small RNAs into AGO2, thereby enhancing plant antiviral resistance. Overall, our study sheds light on the roles of RH3 in RNA silencing and plant antiviral defenses, providing valuable insights into the cytobiological connections between RNA silencing, viral replication, and antiviral immunity. This study reveals that RNA silencing component Argonaute 2 (AGO2) loads small RNAs at endoplasmic reticulum–chloroplast membrane contact sites with the help of RNA helicase 3 (RH3), which also enhances the loading of viral-derived small RNAs. These findings highlight the connections between RNA silencing, viral replication, and plant antiviral resistance.

Argonaute

10.1038/s41467-025-57296-6

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Oxidation treatment of acrylonitrile-containing wastewater by Fenton reagent

Hecheng xiangjiao gongye (2013)

Xing Li-Shu Cong Zhang Yun Fa Juan Du Liang Song

The homemade acrylonitrile(AN)-containing wastewater(100 mg/L) was treated by Fenton reagent oxidation process,the composition of the treated wastewater products was analyzed by gas chromatography,ion chromatography and elemental analysis,and the oxidation reaction mechanism was proposed.The results showed that 200 mL of wastewater was treated by addition of 1-4 mL of H2O2 and 600 mg/L of FeSO4·7 H2O under the conditions of the initial pH value of 1.3-7.0,AN was oxidized to products with different states,including gaseous products with the largest percentage,and some liquid products(including a certain amount of oxalic acid and small amount of acetic acid,glycolic acid,nitrate and ammonium ions) and solid products(including C and N-containing compounds).The AN conversion was over 95%.

Oxalic Acid

Glycolic acid

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Citations (2)