Fathia A. Soudy

Benha University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Baber Ali

Western Sydney University

Doaa Bahaa Eldin Darwish

Mansoura University

Mohammed Ali

Desert Research Center

Dikhnah Alshehri

University of Tabuk

Amany H. A. Abeed

Assiut University

Jian Zhao

Chinese Academy of Fishery Sciences

Rania M. Makki

King Abdulaziz University

Aqsa Hafeez

Quaid-i-Azam University

Mohamed Refaat

Benha University

Muhammad Nauman Khan

Islamia College University

Cooperative Institutions

Benha University

Huazhong Agricultural University

University of the Punjab

King Saud University

Government College University, Faisalabad

Ministry of Agriculture and Rural Affairs

University of Tabuk

King Abdulaziz University

Islamia University of Bahawalpur

Qassim University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Molecular characterization of genes involved in tolerance of cadmium in Triticum aestivum (L.) under Cd stress

Journal of Hazardous Materials (2023)

Asima Rasheed Arwa Abdulkreem AL‐Huqail Baber Ali Suliman Mohammed Suliman Alghanem Asad Ali Shah

10.1016/j.jhazmat.2023.132955

Cite

Citations (10)

Molecular Identification and Characterization of Salmonella Sp. In Raw and Heat-Treated Milks from Local Market

Deleted Journal (2023)

Neven Rashad Mohamed Refaat Elsayed A. Ismail Fathia A. Soudy

Milk is a valuable nutritious food that has a short shelf life and requires careful handling. Therefore, milk is rapidly perishable as it is a suitable medium for the growth of microorganisms particularly; bacterial pathogens that can cause milk spoilage and diseases in consumers. This study aimed to determine the prevalence of Salmonella sp. in raw and heat-treated milk, characterize its virulence-associated genes, and assess its antimicrobial profile. One hundred and thirty samples (50 raw cow's milk, 50 raw buffalo's milk, 10 Heat-treated milk (pasteurized), 20 Heat-treated milk (UHT)) were used for assessing the prevalence of Salmonella sp. Four Salmonella isolates were recovered from Raw cow's milk (4/9; 8%) and five from Raw buffalo's milk (5/9; 10%) while no isolates of salmonella sp. were detected from heat-treated milk. Moreover, the antimicrobial susceptibility testing indicated that all isolates were resistant to Amoxicillin/clavulanic acid (AMC 30) 20/10μg, Erythromycin (E15) 15μg and Cephalexin (CL30) 30μg whilst they are all sensitive to Chloramphenicol (C30) 30μg. The PCR results revealed that the six virulence genes (invA, orgA, sifA, spvA, iroN and spiA) were detected significantly in all nine isolates. Further, the results of ARDRA technique revealed that all isolates belonging to salmonella enterica and was validated using the real time- PCR. Our results indicate that insufficient hygienic measures adopted during handling. Therefore, the strict hygienic approaches are recommended during milking and processing.

Identification

Characterization

10.21608/assjm.2024.253296.1259

Cite

Citations (0)

Changes in Metabolite Profiling and Expression Levels of Key Genes Involved in the Terpenoid Biosynthesis Pathway in Garden Sage (Salvia officinalis) under the Effect of Hydrazine Hydrate

Metabolites (2023)

Mohammed Ali Aisha M. Abdelkawy Doaa Bahaa Eldin Darwish Hanan Ali Alatawi Dikhnah Alshehri

Mutagenesis is a highly efficient tool for establishing genetic variation and is widely used for genetic enhancement in various plants. The key benefit of mutation breeding is the prospect of enhancing one or several characteristics of a variety without altering the genetic background. In this study, we exposed the seeds of Salvia officinalis to four concentrations of hydrazine hydrate (HZ), i.e., (0%, 0.1%, 0.2%, and 0.3%) for 6 h. The contents of terpenoid compounds in the S. officinalis plantlets driven from the HZ-treated seeds were determined by GC-MS, which resulted in the identification of a total of 340 phytochemical compounds; 163 (87.48%), 145 (84.49%), 65 (97.45%), and 62 (98.32%), from the four concentrations of HZ (0%, 0.1%, 0.2%, and 0.3%), respectively. Furthermore, we used the qRT-PCR system to disclose the "transcriptional control" for twelve TPS genes related to terpenoid and terpene biosynthesis, namely, SoGPS, SoMYRS, SoNEOD, SoCINS, SoSABS, SoLINS, SoFPPS, SoHUMS, SoTPS6, SoSQUS, SoGGPS, and SoGA2. Altogether, results are likely to ensure some positive relationship between the concentrations of the chemical mutagen HZ used for treating the seeds, the type and amount of the produced terpenes, and the expression of their corresponding genes.

Salvia officinalis

Terpene

Phytochemical

Melissa Officinalis

Hydrazine (antidepressant)

10.3390/metabo13070807

Cite

Citations (8)

Efficacy of Naphthyl Acetic Acid Foliar Spray in Moderating Drought Effects on the Morphological and Physiological Traits of Maize Plants (Zea maysL.)

ACS Omega (2023)

Sami Ullah Muhammad Ishaq Khan Muhammad Nauman Khan Usman Ali Baber Ali

The threat of varying global climates has greatly driven the attention of scientists, as climate change increases the odds of worsening drought in many parts of Pakistan and the world in the decades ahead. Keeping in view the forthcoming climate change, the present study aimed to evaluate the influence of varying levels of induced drought stress on the physiological mechanism of drought resistance in selected maize cultivars. The sandy loam rhizospheric soil with moisture content 0.43-0.5 g g-1, organic matter (OM) 0.43-0.55 g/kg, N 0.022-0.027 g/kg, P 0.028-0.058 g/kg, and K 0.017-0.042 g/kg was used in the present experiment. The findings showed that a significant drop in the leaf water status, chlorophyll content, and carotenoid content was linked to an increase in sugar, proline, and antioxidant enzyme accumulation at p < 0.05 under induced drought stress, along with an increase in protein content as a dominant response for both cultivars. SVI-I & II, RSR, LAI, LAR, TB, CA, CB, CC, peroxidase (POD), and superoxide dismutase (SOD) content under drought stress were studied for variance analysis in terms of interactions between drought and NAA treatment and were found significant at p < 0.05 after 15 days. It has been found that the exogenous application of NAA alleviated the inhibitory effect of only short-term water stress, but yield loss due to long-term osmotic stress will not be faced employing growth regulators. Climate-smart agriculture is the only approach to reduce the detrimental impact of global fluctuations, such as drought stress, on crop adaptability before they have a significant influence on world crop production.

10.1021/acsomega.3c00753

Cite

Citations (18)

Diversity Analysis for Wheat Genotypes Against Rust Resistant Genes

Polish Journal of Environmental Studies (2024)

Sahir Hameed Khattak Sajid Ali Khan Bangash Imdad Kaleem Sania Begum Saima Noor

The study was designed to evaluate the genetic background of indigenous germplasm by using simple sequence repeats (SSRs) gene markers for the selection of elite lines with multiple gene combinations that can be used in breeding programs for wheat improvement against rust disease.The principal component analysis (PCA) was constructed to estimate the genetic difference between the accessions and revealed an 85.3% variation.A high level of association was found between markers XWMC170 /XGWM608, XGWM44 a /XGWM44 b , XGDM111 a /XGWM129, XWMC765 a /XWMC765 b , and GWM148 a /XWMC765 b while low level of correlation was observed between the markers CSLV34b and CSLV34 a i.e -0.688 followed by GWM148 a and XGWM129 (-0.633).Cluster analysis revealed three

Germ plasm

Rust (programming language)

10.15244/pjoes/173096

Cite

Citations (0)

Genome wide analysis of carotenoid cleavage oxygenases (CCO) gene family in Arachis hypogaea (peanut) under biotic stress

BMC Genomics (2024)

Arsalan Ahmad Adnan Sami Umer Habib Muhammad Ali Muhammad Shafiq

Carotenoid cleavage oxygenases (CCOs) enzymes play a vital role in plant growth and development through the synthesis of apocarotenoids and their derivative. These chemicals are necessary for flower and fruit coloration, as well as the manufacture of plant hormones such as abscisic acid (ABA) and strigolactones, which control a variety of physiological processes. The CCOs gene family has not been characterized in Arachis hypogaea. Genome mining of A. hypogaea identifies 24 AhCCO gene members. The AhCCO gene family was divided into two subgroups based on the recent study of the Arabidopsis thaliana CCO gene family classification system. Twenty-three AhCCO genes, constituting 95.8% of the total, were regulated by 29 miRNAs, underscoring the significance of microRNAs (miRNAs) in governing gene expression in peanuts. AhCCD19 is the only gene that lacks a miRNA target site. The physicochemical characteristics of CCO genes and their molecular weights and isoelectric points were studied further. The genes were then characterized regarding chromosomal distribution, structure, and promoter cis-elements. Light, stress development, drought stress, and hormone responsiveness were discovered to be associated with AhCCO genes, which can be utilized in developing more resilient crops. The investigation also showed the cellular location of the encoded proteins and discovered that the peanut carotenoid oxygenase gene family's expansion was most likely the result of tandem, segmental, and whole-genome duplication events. The localization expresses the abundance of genes mostly in the cytoplasm and chloroplast. Expression analysis shows that AhCCD7 and AhCCD14 genes show the maximum expression in the apical meristem, lateral leaf, and pentafoliate leaf development, while AhNCED9 and AhNCED13 express in response to Aspergillus flavus resistance. This knowledge throws light on the evolutionary history of the AhCCO gene family and may help researchers better understand the molecular processes behind gene duplication occurrences in plants. An integrated synteny study was used to find orthologous carotenoid oxygenase genes in A. hypogaea, whereas Arabidopsis thaliana and Beta vulgaris were used as references for the functional characterization of peanut CCO genes. These studies provide a foundation for future research on the regulation and functions of this gene family. This information provides valuable insights into the genetic regulation of AhCCO genes. This technology could create molecular markers for breeding programs to develop new peanut lines.

Arachis hypogaea

Arachis

Biotic stress

10.1186/s12864-024-10351-w

Cite

Citations (0)

Effective citric acid and EDTA treatments in cadmium stress tolerance in pepper (Capsicum annuum L.) seedlings by regulating specific gene expression

South African Journal of Botany (2023)

Rana M. Alshegaihi Manar Fawzi Bani Mfarrej Muhammad Hamzah Saleem Abida Parveen Khawaja Shafique Ahmad

APX

Ethylenediaminetetraacetic acid

Malondialdehyde

10.1016/j.sajb.2023.06.024

Cite

Citations (41)

Overexpression of Terpenoid Biosynthesis Genes Modifies Root Growth and Nodulation in Soybean (Glycine max)

Cells (2022)

Mohammed Ali Long Miao Fathia A. Soudy Doaa Bahaa Eldin Darwish Salma Saleh Alrdahe

Root nodule formation in many leguminous plants is known to be affected by endogen ous and exogenous factors that affect formation, development, and longevity of nodules in roots. Therefore, it is important to understand the role of the genes which are involved in the regulation of the nodulation signaling pathway. This study aimed to investigate the effect of terpenoids and terpene biosynthesis genes on root nodule formation in Glycine max. The study aimed to clarify not only the impact of over-expressing five terpene synthesis genes isolated from G. max and Salvia guaranitica on soybean nodulation signaling pathway, but also on the strigolactones pathway. The obtained results revealed that the over expression of GmFDPS, GmGGPPS, SgGPS, SgFPPS, and SgLINS genes enhanced the root nodule numbers, fresh weight of nodules, root, and root length. Moreover, the terpene content in the transgenic G. max hairy roots was estimated. The results explored that the monoterpenes, sesquiterpenes and diterpenes were significantly increased in transgenic soybean hairy roots in comparison with the control. Our results indicate the potential effects of terpenoids and terpene synthesis genes on soybean root growth and nodulation. The study provides novel insights for understanding the epistatic relationship between terpenoids, root development, and nodulation in soybean.

Terpene

Root hair

10.3390/cells11172622

Cite

Citations (9)

Molecular Cloning and Expression Analysis of Geranyllinalool Synthase Gene (SgGES) from Salvia guaranitica Plants

Horticulturae (2024)

Ahmed Ali Abdelhameed Mohamed A. Eissa Ragab I. EL-kholy Doaa Bahaa Eldin Darwish Amany H. A. Abeed

Salvia guaranitica is considered one of the most significant medicinal and aromatic herbs in terms of nutritional and medical benefits due to its wealth of important active components. Among these compounds, terpenoids are the most prominent and abundant, particularly monoterpenes (C10), sesquiterpenes (C15), and diterpenes (C20). They are biologically advantageous to plants and perform a multitude of functions. The current study aimed to clone the S. guaranitica gene that encodes for geranyllinalool synthases (SgGES, EC: 4.2.3.144), with consideration for these features. The open reading frame of the 867-amino-acid protein encoded by SgGES consists of 2.721 base pairs. In addition, the SgGES protein has five domains that belong to the terpene synthase family, which are related to the terpene and terpenoid synthase domains. We manipulated and overexpressed the SgGES gene in Nicotiana tabacum to explore its function. When compared to the GUS control, the transgenic N. tabacum plants displayed an increase in leaf production and diameter when compared with the wild-type plants. Finally, analysis of transgenic plants using gas chromatography/mass spectrometry (GC-MS) showed that SgGES is responsible for producing various terpene species, especially diterpenes.

Cloning (programming)

Salvia

10.3390/horticulturae10070668

Cite

Citations (1)

Variability in fecal egg count of Haemonchus contortus infection to native goat breeds of China and Bangladesh under natural grazing condition

Progressive Agriculture (2017)

AI Omar MBB Alam MO Faruque M. Motahar-Hussain Mondal MS Raihan

Haemonchus contortus is a major gastrointestinal nematode affecting goat in China and Bangladesh like many countries in the world. The aim of this study was to evaluate the susceptibility of Haemonchus contortus infection in different goat breeds/populations in China and Bangladesh under the natural grazing condition. Fecal Egg of Haemonchus contortus was counted from 430 goats in both countries using McMasters technique. Body weight, Pack Cell Volume and Hemoglobin value were measured for Bangladeshi goats. Statistical analysis was done after transforming data into log10 (n+1), where n is the number of egg per gram feces and analysis of variance was done by using Generalized Linear Model procedures of computer package of SAS. Prevalence of parasite and Fecal Egg Count varied significantly (P<0.01) in all six goat populations. The highest prevalence (88.89%) of Haemonchus contortus infection was found in Enshi Black and the highest parasitic load (527.74 ±78.13 epg) was found in Chinese hybrid goat population. Most of the individuals, except Chinese hybrid goats, had Fecal Egg Count less than 300 epg. Fecal Egg Count between sexes within breed of goat did not differ significantly (P>0.05). Black Bengal goat in the hilly region of Bangladesh was the least susceptible to Haemonchus contortus infection in term of Fecal Egg Count. Our study suggests that Chinese goat breeds were more susceptible to Haemonchus contortus infection as compared to Black Bengal goat of Bangladesh under natural grazing condition. Further studies on immune-genetics aspects of goats might be helpful to find out the actual causes of such differences and to develop disease resistant breed of goat.Progressive Agriculture 27 (4): 473-481, 2016

Eggs per gram

10.3329/pa.v27i4.32132

Cite

Citations (6)