Two methods were used to prepare the mouse embryonic fibroblast(MEF) feeder layers,and the MEF feeder layers were used to isolate and culture the embryonic stem cells.It was carried out observation to the effect of the various methods on the MEF feeder layers,the effects of the various MEF feeder layers on the development of embryos,the growth of the ICM and the isolation of the embryonic stem(ES)cells,and the optimal passage of the MEF feeder layers were filtered out for preparation of the feeder layers in this experiment.It was find in this experiment that the method for preparation of the MEF feeder layers could simplify the preparation for the MEF feeder layers,but there had no impacts on the isolation the ES cells.
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