Felipe F. Aceituno

Pontificia Universidad Católica de Chile

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Rodrigo A. Gutiérrez

Millennium Institute for Integrative Biology

Francisco Melo

Pontificia Universidad Católica de Chile

Alex W. Slater

Millennium Institute on Immunology and Immunotherapy

Eduardo Agosín

Pontificia Universidad Católica de Chile

Marcelo Orellana

Hospital Posadas

Jorge Torres

Pontificia Universidad Católica de Chile

Dennis Shasha

Courant Institute of Mathematical Sciences

Juan M. Cabello

Pontificia Universidad Católica de Chile

Arthur P. Goldberg

Karolinska Institutet

Rebecca S. Davidson

Courant Institute of Mathematical Sciences

Cooperative Institutions

Pontificia Universidad Católica de Chile

Courant Institute of Mathematical Sciences

Millennium Institute for Integrative Biology

New York University

Centre National de la Recherche Scientifique

Université de Montpellier

University of Chile

Millennium Science Initiative

Agencia Nacional de Investigación y Desarrollo

Helmholtz Zentrum München

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Oxygen Response of the Wine Yeast Saccharomyces cerevisiae EC1118 Grown under Carbon-Sufficient, Nitrogen-Limited Enological Conditions

Applied and Environmental Microbiology (2012)

Felipe F. Aceituno Marcelo Orellana Jorge Torres Sebastián N. Mendoza Alex W. Slater

Discrete additions of oxygen play a critical role in alcoholic fermentation. However, few studies have quantitated the fate of dissolved oxygen and its impact on wine yeast cell physiology under enological conditions. We simulated the range of dissolved oxygen concentrations that occur after a pump-over during the winemaking process by sparging nitrogen-limited continuous cultures with oxygen-nitrogen gaseous mixtures. When the dissolved oxygen concentration increased from 1.2 to 2.7 μM, yeast cells changed from a fully fermentative to a mixed respirofermentative metabolism. This transition is characterized by a switch in the operation of the tricarboxylic acid cycle (TCA) and an activation of NADH shuttling from the cytosol to mitochondria. Nevertheless, fermentative ethanol production remained the major cytosolic NADH sink under all oxygen conditions, suggesting that the limitation of mitochondrial NADH reoxidation is the major cause of the Crabtree effect. This is reinforced by the induction of several key respiratory genes by oxygen, despite the high sugar concentration, indicating that oxygen overrides glucose repression. Genes associated with other processes, such as proline uptake, cell wall remodeling, and oxidative stress, were also significantly affected by oxygen. The results of this study indicate that respiration is responsible for a substantial part of the oxygen response in yeast cells during alcoholic fermentation. This information will facilitate the development of temporal oxygen addition strategies to optimize yeast performance in industrial fermentations.

Winemaking

Mixed acid fermentation

Ethanol Fermentation

Cellular respiration

Wine fault

10.1128/aem.02305-12

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Metabolic and transcriptomic response of the wine yeastSaccharomyces cerevisiaestrain EC1118 after an oxygen impulse under carbon-sufficient, nitrogen-limited fermentative conditions

FEMS Yeast Research (2014)

Marcelo Orellana Felipe F. Aceituno Alex W. Slater Leonardo I. Almonacid Francisco Melo

During alcoholic fermentation, Saccharomyces cerevisiae is exposed to continuously changing environmental conditions, such as decreasing sugar and increasing ethanol concentrations. Oxygen, a critical nutrient to avoid stuck and sluggish fermentations, is only discretely available throughout the process after pump-over operation. In this work, we studied the physiological response of the wine yeast S. cerevisiae strain EC1118 to a sudden increase in dissolved oxygen, simulating pump-over operation. With this aim, an impulse of dissolved oxygen was added to carbon-sufficient, nitrogen-limited anaerobic continuous cultures. Results showed that genes related to mitochondrial respiration, ergosterol biosynthesis, and oxidative stress, among other metabolic pathways, were induced after the oxygen impulse. On the other hand, mannoprotein coding genes were repressed. The changes in the expression of these genes are coordinated responses that share common elements at the level of transcriptional regulation. Beneficial and detrimental effects of these physiological processes on wine quality highlight the dual role of oxygen in 'making or breaking wines'. These findings will facilitate the development of oxygen addition strategies to optimize yeast performance in industrial fermentations.

Strain (injury)

10.1111/1567-1364.12135

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VirtualPlant: A Software Platform to Support Systems

Manpreet S. Katari David Nowicki Felipe F. Aceituno Damion Nero Jonathan Kelfer

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Systems approach identifies TGA1 and TGA4 transcription factors as important regulatory components of the nitrate response of Arabidopsis thaliana roots

The Plant Journal (2014)

José M. Álvarez Eleodoro Riveras Elena A. Vidal Diana E. Gras Orlando Contreras‐López

Summary Nitrate acts as a potent signal to control global gene expression in A rabidopsis. Using an integrative bioinformatics approach we identified TGA 1 and TGA 4 as putative regulatory factors that mediate nitrate responses in A rabidopsis roots. We showed that both TGA 1 and TGA 4 m RNA s accumulate strongly after nitrate treatments in roots. Global gene expression analysis revealed 97% of the genes with altered expression in tga1 tga4 double mutant plants respond to nitrate treatments, indicating that these transcription factors have a specific role in nitrate responses in Arabidopsis root organs. We found TGA 1 and TGA 4 regulate the expression of nitrate transporter genes NRT 2.1 and NRT 2.2 . Specific binding of TGA 1 to its cognate DNA sequence on NRT 2.1 and NRT 2.2 promoters was confirmed by chromatin immunoprecipitation assays. The tga1 tga4 double mutant plants exhibit nitrate‐dependent lateral and primary root phenotypes. Lateral root initiation is affected in both tga1 tga4 and nrt1.2 nrt2.2 double mutants, suggesting TGA 1 and TGA 4 regulate lateral root development at least partly via NRT 2.1 and NRT 2.2. Additional root phenotypes of tga1 tga4 double mutants indicate that these transcription factors play an important role in root developmental responses to nitrate. These results identify TGA 1 and TGA 4 as important regulatory factors of the nitrate response in A rabidopsis roots.

Chromatin immunoprecipitation

Lateral root

Transcription

10.1111/tpj.12618

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Citations (271)

VirtualPlant: A Software Platform to Support Systems Biology Research

PLANT PHYSIOLOGY (2009)

Manpreet S. Katari Steve D. Nowicki Felipe F. Aceituno Damion Nero Jonathan Kelfer

Abstract Data generation is no longer the limiting factor in advancing biological research. In addition, data integration, analysis, and interpretation have become key bottlenecks and challenges that biologists conducting genomic research face daily. To enable biologists to derive testable hypotheses from the increasing amount of genomic data, we have developed the VirtualPlant software platform. VirtualPlant enables scientists to visualize, integrate, and analyze genomic data from a systems biology perspective. VirtualPlant integrates genome-wide data concerning the known and predicted relationships among genes, proteins, and molecules, as well as genome-scale experimental measurements. VirtualPlant also provides visualization techniques that render multivariate information in visual formats that facilitate the extraction of biological concepts. Importantly, VirtualPlant helps biologists who are not trained in computer science to mine lists of genes, microarray experiments, and gene networks to address questions in plant biology, such as: What are the molecular mechanisms by which internal or external perturbations affect processes controlling growth and development? We illustrate the use of VirtualPlant with three case studies, ranging from querying a gene of interest to the identification of gene networks and regulatory hubs that control seed development. Whereas the VirtualPlant software was developed to mine Arabidopsis (Arabidopsis thaliana) genomic data, its data structures, algorithms, and visualization tools are designed in a species-independent way. VirtualPlant is freely available at www.virtualplant.org.

Identification

Biological data

10.1104/pp.109.147025

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3-Chlorobenzoate is taken up by a chromosomally encoded transport system in Cupriavidus necator JMP134

Microbiology (2009)

Thomas Ledger Felipe F. Aceituno Bernardo González

Cupriavidus necator JMP134(pJP4) is able to grow on 3-chlorobenzoate (3-CB), a model chloroaromatic pollutant. Catabolism of 3-CB is achieved via the expression of the chromosomally encoded benABCD genes and the tfd genes from plasmid pJP4. Since passive diffusion of benzoic acid derivatives at physiological pH is negligible, the uptake of this compound should be facilitated by a transport system. However, no transporter has so far been described to perform this function, and identification of chloroaromatic compound transporters has been limited. In this work, uptake experiments using 3-[ring-UL-(14)C]CB showed an inducible transport system in strain JMP134, whose expression is activated by 3-CB and benzoate. A similar level of 3-CB uptake was found for a mutant strain of JMP134, defective in chlorobenzoate degradation, indicating that metabolic drag is not an important component of the measured uptake rate. Competitive inhibitor assays showed that uptake of 3-CB was inhibited by benzoate and, to a lesser degree, by 3-CB and 3,5-dichlorobenzoate, but not by any of 12 other substituted benzoates tested. The expression of several gene candidates for this transport function was analysed by RT-PCR, including both permease-type and ABC-type ATP-dependent transporters. Induction of a chromosomally encoded putative permease transporter (benP gene) was found specifically in the presence of 3-CB or benzoate. A benP knockout mutant of strain JMP134 displayed an almost complete loss of 3-CB transport activity. This is to our knowledge the first report of a 3-CB transporter.

Cupriavidus necator

Benzoates

10.1099/mic.0.029207-0

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A composite transcriptional signature differentiates responses towards closely related herbicides in Arabidopsis thaliana and Brassica napus

Plant Molecular Biology (2009)

Malay Das Jay R. Reichman Georg Haberer Gerhard Welzl Felipe F. Aceituno

In this study, genome-wide expression profiling based on Affymetrix ATH1 arrays was used to identify discriminating responses of Arabidopsis thaliana to five herbicides, which contain active ingredients targeting two different branches of amino acid biosynthesis. One herbicide contained glyphosate, which targets 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), while the other four herbicides contain different acetolactate synthase (ALS) inhibiting compounds. In contrast to the herbicide containing glyphosate, which affected only a few transcripts, many effects of the ALS inhibiting herbicides were revealed based on transcriptional changes related to ribosome biogenesis and translation, secondary metabolism, cell wall modification and growth. The expression pattern of a set of 101 genes provided a specific, composite signature that was distinct from other major stress responses and differentiated among herbicides targeting the same enzyme (ALS) or containing the same chemical class of active ingredient (sulfonylurea). A set of homologous genes could be identified in Brassica napus that exhibited a similar expression pattern and correctly distinguished exposure to the five herbicides. Our results show the ability of a limited number of genes to classify and differentiate responses to closely related herbicides in A. thaliana and B. napus and the transferability of a complex transcriptional signature across species.

Acetolactate synthase

Secondary metabolism

10.1007/s11103-009-9590-y

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Citations (65)

The rules of gene expression in plants: Organ identity and gene body methylation are key factors for regulation of gene expression in Arabidopsis thaliana

BMC Genomics (2008)

Felipe F. Aceituno Nick Moseyko Seung Y. Rhee Rodrigo A. Gutiérrez

Microarray technology is a widely used approach for monitoring genome-wide gene expression. For Arabidopsis, there are over 1,800 microarray hybridizations representing many different experimental conditions on Affymetrix ATH1 gene chips alone. This huge amount of data offers a unique opportunity to infer the principles that govern the regulation of gene expression in plants.We used bioinformatics methods to analyze publicly available data obtained using the ATH1 chip from Affymetrix. A total of 1887 ATH1 hybridizations were normalized and filtered to eliminate low-quality hybridizations. We classified and compared control and treatment hybridizations and determined differential gene expression. The largest differences in gene expression were observed when comparing samples obtained from different organs. On average, ten-fold more genes were differentially expressed between organs as compared to any other experimental variable. We defined "gene responsiveness" as the number of comparisons in which a gene changed its expression significantly. We defined genes with the highest and lowest responsiveness levels as hypervariable and housekeeping genes, respectively. Remarkably, housekeeping genes were best distinguished from hypervariable genes by differences in methylation status in their transcribed regions. Moreover, methylation in the transcribed region was inversely correlated (R2 = 0.8) with gene responsiveness on a genome-wide scale. We provide an example of this negative relationship using genes encoding TCA cycle enzymes, by contrasting their regulatory responsiveness to nitrate and methylation status in their transcribed regions.Our results indicate that the Arabidopsis transcriptome is largely established during development and is comparatively stable when faced with external perturbations. We suggest a novel functional role for DNA methylation in the transcribed region as a key determinant capable of restraining the capacity of a gene to respond to internal/external cues. Our findings suggest a prominent role for epigenetic mechanisms in the regulation of gene expression in plants.

Housekeeping gene

10.1186/1471-2164-9-438

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Citations (115)

Running Title: Impact of oxygen on wine yeast physiology. 3

Felipe F. Aceituno Marcelo Orellana Jorge Torres Sebastián N. Mendoza Alex W. Slater

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