Fatemeh Eini

Hormozgan University of Medical Sciences

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Marefat Ghaffari Novin

Shahid Beheshti University of Medical Sciences

M. Azizi

Hormozgan University of Medical Sciences

Hamid Nazarian

Shahid Beheshti University of Medical Sciences

Arash Bidadkosh

Children's Hospital at Westmead

Maryam Hosseinzadeh Shirzeyli

Shahid Beheshti University of Medical Sciences

Abbas Piryaei

Shahid Beheshti University of Medical Sciences

Khojasteh Joharchi

Shahid Beheshti University of Medical Sciences

Ensieh Salehi

Hormozgan University of Medical Sciences

Keivan Majidzadeh‐A

Motamed Cancer Institute

Tahereh Foroutan

Kharazmi University

Cooperative Institutions

Shahid Beheshti University of Medical Sciences

Hormozgan University of Medical Sciences

Tehran University of Medical Sciences

University of Tehran

Tarbiat Modares University

Isfahan University of Medical Sciences

Shahid Beheshti University

Shiraz University of Medical Sciences

Academic Center for Education, Culture and Research

Iran University of Medical Sciences

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Improvement in the epigenetic modification and development competence in PCOS mice oocytes by hydro-alcoholic extract of Nigella sativa during in-vitro maturation: An experimental study

International Journal of Reproductive BioMedicine (IJRM) (2020)

Fatemeh Eini Khojasteh Joharchi M. Azizi Pegah Mousavi

Nigella Sativa (NS) and its active component, thymoquinone, have beneficial protective effects on experimental animal models of polycystic ovary syndrome (PCOS) and different human diseases.The present study aimed to investigate the effects of NS hydro-alcoholic extract (NSE) on the oocyte quality of PCOS mice during in vitro maturation.For induction of PCOS, 40 prepubertal 21-days old female B6D2F1 mice (18-22 g body weight) received subcutaneous dehydroepiandrosterone daily. After validation of the model, germinal vesicle-stage oocytes of superovulated mice were collected and placed in the culture medium containing different concentrations (0, 1, 50, and 100 μg/ml) of NSE. For the measurement of developmental competency, some mature oocytes were fertilized with epididymal spermatozoa. Other mature oocytes were assessed for oxidative stress. Also, some mRNA expression levels involved in oocyte maturation and epigenetic modification were evaluated.The 50 μg/ml NSE treated group showed significantly higher r ates o f maturation, f ertilization, and blastocyst formation in comparison with both control and PCOS groups. A high level of glutathione concentration and glutathione peroxidase mRNA expression, besides a low level of reactive oxygen species content all, were observed in oocytes treated with 50 μg/ml NSE, indicating the modification of oxidative statue. Furthermore, the oocytes in the 50 μg/ml-treated group showed an upregulation of mRNA expression in epigenetic-related genes (Dnmt1 and Hdac1) and maternally derived genes (Mapk and Cdk1), correspondingly downregulation of cyclooxygenase2 mRNA expression, in comparison to other groups.The results of this study indicated that 50 μg/ml NSE improves oocyte maturation, oxidative statues and epigenetic modifications. These may be the all reasons for the developmental competency in the control and PCOS mice oocytes.

Germinal vesicle

In vitro maturation

10.18502/ijrm.v13i9.7668

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Citations (13)

Effect of bacterial infection on sperm quality and DNA fragmentation in subfertile men with Leukocytospermia

BMC Molecular and Cell Biology (2021)

Fatemeh Eini M. Azizi F Zareei Zeinolabedin Sharifian Dastjerdi Maryam Hosseinzadeh Shirzeyli

Although bacterial infections have been recognized as a possible cause of male infertility, the effect of bacterial infections on sperm quality and sperm DNA fragmentation remains controversial. The current study aimed to investigate the prevalence rate of bacterial infection in subfertile men and its effect on semen quality. Seminal fluid was collected from 172 male members of infertile couples attending the andrology infertility center and a group of 35 fertile subjects as a control. Sperm parameters and DNA fragmentation were evaluated based on the type of bacteria in all ejaculates.From the 172 patients investigated for infertility, 60 (34.88%) patients had a positive culture for pathogenic bacteria of different species. Leukocytospermia was significantly higher in infected samples in comparison with non-infected samples (p < 0.05). Sperm concentration and motility and morphology were significantly lower in infected than non-infected samples. Moreover, sperm DNA fragmentation was significantly higher in infected than non-infected samples. Besides, our results showed that sperm DNA fragmentation was correlated significantly with leukocytospermia (R: 0.22, p < 0.01).The present study suggested that bacterial infection significantly correlated with leukocytospermia could impair male fertility potential through decreasing sperm motility, morphology, and DNA integrity.

Semen quality

10.1186/s12860-021-00380-8

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The Association of Hyperlipidemia at Late Pregnancy With Maternal and Neonatal Outcomes in Women With Gestational Diabetes

Disease and Diagnosis (2024)

M. Azizi Firoozeh Shakeri Fatemeh Eini Nahid Shakeri Ensieh Salehi

Background: There is evidence that hyperlipidemia during early pregnancy is linked to adverse consequences for expecting mothers and newborns. This study assessed how hyperlipidemia in the third trimester affected outcomes in pregnant women with gestational diabetes mellitus (GDM). Materials and Methods: This study was conducted on 116 pregnant women with GDM.Maternal serum lipids were measured in the first and third trimesters of gestation. The participants were split into two groups: pregnant women with GDM and hyperlipidemia as the hyperlipidemia group (n=58) and pregnant women with only GDM as the non-hyperlipidemia group (n=58). The association between dyslipidemia and maternal and neonatal outcomes was evaluated. Results: Significant differences were observed between the two groups regarding maternal serum lipids at late gestation, body mass index (BMI), mean neonatal weight, preeclampsia, fetal macrosomia, and cesarean section. Maternal dyslipidemia was significantly related to increased odds of cesarean section (OR=2.622, CI:1.170-5.876, P=0.019) and preeclampsia (OR=4.452, CI:1.719-11.530, P=0.002). Newborns in the hyperlipidemia group had lower 1-minute Apgar scores than those in the non-hyperlipidemia group (P<0.001). Moreover, the risk of fetal macrosomia was 5.833 times higher in the hyperlipidemia group than in the non-hyperlipidemia group (OR=5.833, CI: 1.576-21.586, P=0.008). Conclusion: Hyperlipidemia at late gestation is related to complications and unfavorable maternal and neonatal outcomes. The evaluation of lipid profiles before and during pregnancy is needed to diagnose and manage maternal and neonatal complications, especially in high-risk populations like women with GDM.

Hyperlipidemia

Dyslipidemia

Gestational hypertension

10.34172/ddj.552

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Thymoquinone reduces intracytoplasmic oxidative stress and improves epigenetic modification in polycystic ovary syndrome mice oocytes, during in‐vitro maturation

Molecular Reproduction and Development (2019)

Fatemeh Eini Arash Bidadkosh Hamid Nazarian Abbas Piryaei Marefat Ghaffari Novin

Abstract Although in‐vitro maturation (IVM) of oocytes has been presented as an alternative treatment to traditional stimulated in‐vitro fertilization, the culture condition can be improved by natural antioxidants. Thus, we investigated the protective effect of Thymoquinone (TQ) during IVM in the polycystic ovary syndrome (PCOS) mice model. The induction of PCOS was made by dehydroepiandrosterone via subcutaneous injection, in prepubertal female B6D2F1‐mice. After 21 days later, germinal vesicle (GV)‐stage‐oocytes were extracted and incubated in IVM media containing 0, 1.0, 10.0, and 100.0 μM of TQ. To assess fertilization and blastulation rates, after 22–24 hr, the treated oocytes were fertilized in‐vitro with epididymal spermatozoa. Some other oocytes were evaluated for maturation, epigenetic, and oxidative stress markers. Similarly, the mRNA expression of epigenetic enzymes genes (Dnmt1 and Hdac1), three maternally derived genes (Mapk, CyclinB, and Cdk1) and apoptosis‐related genes (Bax and Bcl2) were assessed. Our results showed that the maturation, fertilization, and blastulation rates were significantly higher in the 10.0 μM TQ‐treated group compared with the untreated group and likewise with in‐vivo matured oocytes. The Bax expression was reduced in 10.0 μM TQ matured oocytes, but Bcl2, Dnmt1, Hdac1, Cdk1, and Mapk were upregulated in this group compared to other groups. Furthermore, dimethylation of histone‐3 at lysine‐9 (H3K9m2) and DNA methylation were significantly increased whereas H4K12 acetylation (H4K12ac) was decreased in the 10.0 μM TQ‐treated group in comparison with control and in‐vivo matured oocytes. Therefore, our results are suggesting that 10.0 μM TQ may enhance the developmental competence of PCOS oocytes via the modulation of oxidative stress and epigenetic alterations.

Germinal vesicle

In vitro maturation

10.1002/mrd.23222

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Design of improved polymerase chain reaction (PCR) method containing internal positive control (IPC) for molecular detection of Yersinia pestis

International Journal of Infectious Diseases (2010)

Fatemeh Eini M. Fallah Raoufi Milad Soleimani F. Azarifard E. Jamshidiyan

Background: Plague is one of the three epidemic diseases still subject to the international health regulation and notifiable to the WHO. Yersinia pestis, the causative agent of plague in natural foci transmitted between rodent and human via wild rodent fleas. So set up of suitable and sure to surveillance and prevention of the disease is necessary. The molecular methods such as polymerase chaine reaction (PCR) has allowed improvement of detection methods currently used in laboratories, although not all of these methods include an Internal Positive Control (IPC) to monitor for false negative results. Therefore we improved a uniplex and multiplex PCR method with lower of limit of detection. Methods: PCR reactions performed with primers which targeted of the caf1 and pla genes located on the pFra and pPst plasmids and the irp2 chromosomal gene located on the 'pathogenicity island. For acquired different size of these genes aditional primers were designed. In each gene two parts required were ligated.The limit of detection determined by performing PCR reactions on serial dilutions of plasmids containing the coresponding inserts.for evaluating the specificity, PCR reactions were done for negative control bacteria. Results: Assays were performed with genome of Y. pestis which produced three DNA fragments of the expected size 300, 400 and 520 base pairs (bp) corresponding to irp2, caf1 and pla genes respectively. also, for IPC by the same primers the different fragment of the expected size 150, 500 and 300 base pairs (bp) corresponding to irp2, caf1 and pla genes respectively were acquired. With The lower limit of detection was 370 copy numbers for caf1 gene and 21 for pla gene. In PCR reactions for negative control bacteria detectable fragments were not observed. Conclusion: Our method clearly discriminated Y. pestis DNA bacteria which were tested. The rapidity, specificity and sensitivity of this procedure with the use of IPC to monitor for false negative results can make this method suitable for diagnosis and suggest that it can serve as a useful alternative method for inoculation of laboratory animals or the use of specific culture media for routine plaque surveillance and outbreak investigations. Abstracts for SupplementInternational Journal of Infectious DiseasesVol. 14Preview Full-Text PDF Open Archive

Yersinia pestis

Primer (cosmetics)

plague

10.1016/j.ijid.2010.02.427

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Cover Image, Volume 86, Issue 8, August 2019

Molecular Reproduction and Development (2019)

Fatemeh Eini Arash Bidadkosh Hamid Nazarian Abbas Piryaei Marefat Ghaffari Novin

Cover Caption: The cover image is based on the Research Article Thymoquinone reduces intracytoplasmic oxidative stress and improves epigenetic modification in polycystic ovary syndrome mice oocytes, during in-vitro maturation by Fatemeh Eini et al., https://doi.org/10.1002/mrd.23222. Credit: Cover image © Dr. Fatemeh Eini Images.

10.1002/mrd.23023

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Comparison of the efficacy of bone morphogenetic protein-4 on in vitro differentiation of murine adipose and bone marrow mesenchymal stem cells into primordial germ cells

Research in Pharmaceutical Sciences (2022)

Maryam Hosseinzadeh Shirzeyli Ali Tayyebiazar Fereshteh Aliakbari Fahimeh Ghasemi Fatemeh Eini

In vitro development of functional gametes from pluripotent stem cells is a promising prospect to treat infertility. Mesenchymal stem cells with a high degree of plasticity and less tumorigenicity are a reliable source of stem cells for the generation of gametes. The present study aimed to compare the differentiation potential in the mesenchymal stem cells that are derived from bone marrow (BMDMSCs) and adipose tissue derived mesenchymal stem cells (AD-MSCs) into germ cells in a culture medium containing bone morphogenic protein-4 (BMP-4).In this study, MSCs were isolated from both bone marrow and adipose tissue of murine samples. To further verify the nature of the harvested stem cells, their multipotency and surface marker were examined. The identified stem cells were cultured in a medium supplemented with 0 and 25 ng/mL of BMP-4 for 4 days. Flow cytometry analysis, immunofluorescence staining, and real RT-PCR were used to assess the expression levels in germ cell-specific biomarkers (Mvh, Dazl, Stra8, and Scp3).CD44+, CD45-, CD31-, BMD-MSCs, and AD-MSCs showed to be capable of differentiating to osteo-adipogenic lineages. The flow cytometry, immunofluorescence, and RT-PCR results indicated that early germ cell markers (Mvh and Dazl) were expressed in both types of cells but they were significantly higher in BMD-MSCs than AD-MSCs.Based on our results, the addition of exogenous BMP4 to the culture medium could differentiate BMD-MSCs and AD-MSCs into primordial germ cells, but it is inadequate to further develop into late germ cells in vitro. Moreover, the results revealed that, although AD-MSCs were easier to collect and had faster growth and proliferation rates than BMD-MSCs, the BMD-MSCs were better capable of differentiation into primordial germ cells. They may serve to be considered a more suitable source of MSC for in vitro generation of gametes than AD-MSCs.

Amniotic stem cells

10.4103/1735-5362.335171

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Citations (1)

Intracytoplasmic oxidative stress reverses epigenetic modifications in polycystic ovary syndrome

Reproduction Fertility and Development (2017)

Fatemeh Eini Marefat Ghaffari Novin Khojasteh Joharchi Ahmad Hosseini Hamid Nazarian

In polycystic ovary syndrome (PCOS), substantial genetic and environmental alterations, along with hyperandrogenism, affect the quality of oocytes and decrease ovulation rates. To determine the mechanisms underlying these alterations caused specifically by an increase in plasma androgens, the present study was performed in experimentally-induced PCOS mice. As the study model, female B6D2F1 mice were treated with dehydroepiandrosterone (DHEA, 6mg per 100g bodyweight). After 20 days, oocytes at the germinal vesicle and metaphase II stages were retrieved from isolated ovaries and subsequent analyses of oocyte quality were performed for each mouse. DHEA treatment resulted in excessive abnormal morphology and decreased polar body extrusion rates in oocytes, and was associated with an increase in oxidative stress. Analysis of fluorescence intensity revealed a significant reduction of DNA methylation and dimethylation of histone H3 at lysine 9 (H3K9) in DHEA-treated oocytes, which was associated with increased acetylation of H4K12. Similarly, mRNA expression of DNA methyltransferase-1 and histone deacetylase-1 was significantly decreased in DHEA-treated mice. There was a significant correlation between excessive reactive oxygen species (ROS) production and increased histone acetylation, which is a novel finding and may provide new insights into the mechanism causing PCOS. The results of the present study indicate that epigenetic modifications of oocytes possibly affect the quality of maturation and ovulation rates in PCOS, and that the likely mechanism may be augmentation of intracytoplasmic ROS.

Germinal vesicle

10.1071/rd16428

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The effects of freeze/thawing process on cryopreserved equine umbilical cord blood-derived mesenchymal stem cells

Comparative Clinical Pathology (2011)

Fatemeh Eini Tahereh Foroutan Arash Bidadkosh Abbas Barin Mohammad Mehdi Dehghan

Cord lining

Trypan blue

Cord blood

Viability assay

10.1007/s00580-011-1355-8

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Citations (8)

The effects of myo-inositol vs. metformin on the ovarian function in the polycystic ovary syndrome: a systematic review and meta-analysis.

DOAJ (DOAJ: Directory of Open Access Journals) (2021)

M. Azizi Saeed Hosseini Teshnizi Pearl Ghaemmaghami Fatemeh Eini Nasibeh Roozbeh

Recent studies have revealed that myo-inositol could be more influential in patients with polycystic ovary syndrome (PCOS). This study was aimed to determine and compare the effects of myo-inositol and metformin on hormonal and metabolic profiles and fertility outcomes.A comprehensive search was carried out among the English-language databases, including PubMed, Scopus, Cochrane Library, Google Scholar, and Web of Science, and the articles published from April 2010 to February 2019 were tracked down. The fixed and random-effects meta-analysis was used to estimate the pooled effect size. The meta-analysis was performed in Stata Version 14.0.Nine studies with 331 patients treated with metformin and 307 patients treated with myo-inositol groups were included in the analysis. The research groups did not diverge significantly in terms of the basic characteristics, such as age and Body Mass Index (BMI). In the myo-inositol group, the levels of Luteinizing Hormone (LH) [12.55% (95% I: 11.41-13.68%)], S. testosterone [44.38% (95% CI: 38.09-50.67%)] and prolactin [7.97% (95% CI: 6.58- 9.37%)] were significantly higher than those recorded, i.e., LH [7.97% (95% CI: 6.58- 9.37%)], S. testosterone [8.48% (95% CI: 3.14-13.83%)] and prolactin [7.14% (95% CI: 1.50-14.79%)] for the metformin group (p<0.001).Due to the dearth of related research and the high heterogeneity of the Randomized Clinical Trials (RCTs) included in other studies, the present systematic review could not establish any differences between metformin and myo-inositol concerning the hormonal profile and the ovarian function. However, the findings indicated that myo-inositol could improve fertility outcomes by modulating hyperandrogenism. Randomized trials are required to understand the mechanistic actions of myo-inositol in comparison with those of metformin regarding oocyte and embryo quality, fertilization, pregnancy, and live birth rates.

10.26355/eurrev_202104_25565

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Citations (16)