Yozo Nakazawa

Shinshu University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Impact of infliximab administration before plasma exchange therapy on patients with Kawasaki disease

Therapeutic Apheresis and Dialysis (2020)

Shun Shimada Daisuke Matsuoka Tsubasa Murase Akira Hachiya Noriko Motoki

Abstract Plasma exchange is a therapeutic option in refractory Kawasaki disease (KD). However, the effects of other immunosuppressive treatments on plasma exchange therapy have not been studied. We investigated the effect of infliximab on plasma exchange in KD as well as on the outcome in patients with KD. We studied 16 patients with intravenous immunoglobulin‐resistant KD who finally underwent plasma exchange. The patients were divided into two groups: patients who received infliximab before plasma exchange (infliximab group) and patients who did not (non‐infliximab group). The infliximab group showed a lesser median number of required total plasma exchange sessions ( P = .002) and higher change and reduction rates in C‐reactive protein before and after the first plasma exchange (both P = .027) than that of the non‐infliximab group. Infliximab administered before plasma exchange reduced the number of total plasma exchange sessions and improved the plasma exchange efficacy.

Therapeutic plasma exchange

Refractory (planetary science)

10.1111/1744-9987.13486

Cite

Citations (5)

Significance of chemokine receptor expression in aggressive NK cell leukemia

Leukemia (2005)

Hideki Makishima Toshiro Ito Naoki Asano Hideyuki Nakazawa Shigetaka Shimodaira

Lymphocytosis

CC chemokine receptors

10.1038/sj.leu.2403732

Cite

Citations (34)

PiggyBac-mediated Cancer Immunotherapy Using EBV-specific Cytotoxic T-cells Expressing HER2-specific Chimeric Antigen Receptor

Molecular Therapy (2011)

Yozo Nakazawa Leslie E. Huye Vita S. Salsman Ann M. Leen Nabil Ahmed

Epstein-Barr virus (EBV)-specific cytotoxic T lymphocytes (CTLs) can be modified to function as heterologous tumor directed effector cells that survive longer in vivo than tumor directed T cells without virus specificity, due to chronic stimulation by viral antigens expressed during persistent infection in seropositive individuals. We evaluated the nonviral piggyBac (PB) transposon system as a platform for modifying EBV-CTLs to express a functional human epidermal growth factor receptor 2-specific chimeric antigen receptor (HER2-CAR) thereby directing virus-specific, gene modified CTLs towards HER2-positive cancer cells. Peripheral blood mononuclear cells (PBMCs) were nucleofected with transposons encoding a HER2-CAR and a truncated CD19 molecule for selection followed by specific activation and expansion of EBV-CTLs. HER2-CAR was expressed in ~40% of T cells after CD19 selection with retention of immunophenotype, polyclonality, and function. HER2-CAR-modified EBV-CTLs (HER2-CTLs) killed HER2-positive brain tumor cell lines in vitro, exhibited transient and reversible increases in HER2-CAR expression following antigen-specific stimulation, and stably expressed HER2-CAR beyond 120 days. Adoptive transfer of PB-modified HER2-CTLs resulted in tumor regression in a murine xenograft model. Our results demonstrate that PB can be used to redirect virus-specific CTLs to tumor targets, which should prolong tumor-specific T cell survival in vivo producing more efficacious immunotherapy. Epstein-Barr virus (EBV)-specific cytotoxic T lymphocytes (CTLs) can be modified to function as heterologous tumor directed effector cells that survive longer in vivo than tumor directed T cells without virus specificity, due to chronic stimulation by viral antigens expressed during persistent infection in seropositive individuals. We evaluated the nonviral piggyBac (PB) transposon system as a platform for modifying EBV-CTLs to express a functional human epidermal growth factor receptor 2-specific chimeric antigen receptor (HER2-CAR) thereby directing virus-specific, gene modified CTLs towards HER2-positive cancer cells. Peripheral blood mononuclear cells (PBMCs) were nucleofected with transposons encoding a HER2-CAR and a truncated CD19 molecule for selection followed by specific activation and expansion of EBV-CTLs. HER2-CAR was expressed in ~40% of T cells after CD19 selection with retention of immunophenotype, polyclonality, and function. HER2-CAR-modified EBV-CTLs (HER2-CTLs) killed HER2-positive brain tumor cell lines in vitro, exhibited transient and reversible increases in HER2-CAR expression following antigen-specific stimulation, and stably expressed HER2-CAR beyond 120 days. Adoptive transfer of PB-modified HER2-CTLs resulted in tumor regression in a murine xenograft model. Our results demonstrate that PB can be used to redirect virus-specific CTLs to tumor targets, which should prolong tumor-specific T cell survival in vivo producing more efficacious immunotherapy.

Cancer Immunotherapy

Adoptive Cell Transfer

10.1038/mt.2011.131

Cite

Citations (123)

Delayed methotrexate clearance and acute kidney injury after high‐dose methotrexate chemotherapy concurrent with dasatinib in a patient with relapsed Philadelphia chromosome‐positive acute lymphoblastic leukemia: A case report

Pediatric Blood & Cancer (2020)

Haruka Morota Shoji Saito Eriko Uchida Eri Okura Daisuke Matsuoka

The authors declare that there is no conflict of interest. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.

Philadelphia chromosome

10.1002/pbc.28173

Cite

Citations (2)

Bacteremia due to Moraxella osloensis : a case report and literature review

The Brazilian Journal of Infectious Diseases (2018)

Y. Maruyama Tomonari Shigemura Koki Aoyama Noriyuki Nagano Yozo Nakazawa

Herein we report the case of a 10-year-old boy with an autosomal mosaic mutation who developed bacteremia. The causative agent was identified as Moraxella osloensis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and 16S rRNA gene sequencing. In the pediatric population, there have been 13 case reports of infection attributed to M. osloensis and this is the fifth reported case of pediatric bacteremia due to M. osloensis. After Moraxella species infection was confirmed, the patient recovered with appropriate antimicrobial therapy. It is important to consider that M. osloensis can cause serious infections, such as bacteremia, in otherwise healthy children.

Bacteremia

Moraxella (Branhamella) catarrhalis

10.1016/j.bjid.2017.10.008

Cite

Citations (10)

Electronic Nose and Chemical Analyses of Commercial Fish Sauce Products Show Country-Specific Preferences

food preservation science (2018)

Mitsutoshi Nakano Yoshimasa Sagane Ryosuke Koizumi Kiyoharu Ikehama Koichi YOSHIDA

魚醤は,その独特な風味から特に東南アジア諸国では広く利用される調味料である。熟成させることで魚肉中のタンパク質が分解され,呈味性成分が増し,さらに耐塩性微生物の作用により,その香りが形成される。本研究では,市販されている日本,タイ,ベトナム,中国,フィリピンおよびイタリア産の46種類の魚醤についてその化学的特性と香りの分析を行った。その結果,魚醤は,塩濃度,タンパク質分解産物,酸成分,そして香りに顕著な多様性がみられた。これらの多様性は,それぞれの国での魚醤の製造法の違いによるもの,そしてさらには,それぞれの国の消費者に特有な味や香りへの嗜好性や許容性を反映したものであると考えられた。

Electronic Nose

10.5891/jafps.44.125

Cite

Citations (0)

Extraction of Phospholipids from the Unused Resources of Squid

food preservation science (2018)

Yozo Nakazawa Ayano FUKUI Masao Yamazaki Hiroaki Sato

An efficient method to extract phospholipids (PLs) from the residues of squid processing was studied. Considering its application to various foods, PL extraction with hexane-ethanol (HE) was tested and shown to achieve 75%-85% extraction efficiency compared to that with chloroform-methanol. Analysis of the lipid composition using thin-layer chromatography (TLC) revealed that 51.3% of lipids in the internal organs of squids (goro) were triacylglycerols (TGs), whereas PLs accounted for only 19.2%. In contrast, the lipid composition in squid arms (geso), mantle hemlines (suso), and fins (mimi) was similar. In squid arms, the TG content was extremely low at 3.3% and the PL content was as high as 50.9%. The HE extract from geso was subjected to acetone fractionation and preparative TLC to obtain highly purified PLs. The purified PLs were analyzed by reversed-phase high-performance liquid chromatography to determine the fatty acid composition and found to contain 10.8% icosapentaenoic acid and 19.0% docosahexaenoic acid, which are highly unsaturated fatty acids. When converted into the extracted amount per 1.0 kg fresh Japanese common squid, we found that 6.7 and 8.0g PLs could be extracted from goro and geso, respectively.

10.5891/jafps.44.9

Cite

Citations (1)

Clinical Feature and Genetic Alterations in Myeloid/Natural Killer (NK) Cell Precursor Acute Leukemia and Myeloid/NK Cell Acute Leukemia

Blood (2018)

Akira Nishimura Kazuaki Yokoyama Chika Yamagishi Takuya Naruto Tomohiro Morio

Hematology

10.1182/blood-2018-99-118627

Cite

Citations (0)

Impact of AKI on OS after allo-HSCT_dataset_20210123

Figshare (2021)

Koichi Hirabayashi Yozo Nakazawa Shoji Saito Daisuke Matsuoka

Dataset for "Impact of acute kidney injury on overall survival in children and young adults undergoing allogeneic hematopoietic stem cell transplantation".

10.6084/m9.figshare.13632425

Cite

Citations (0)

Domestic CAR T cells for myeloid neoplasms

The Japanese Society of Hematology (2018)

Yozo Nakazawa

Source

Cite

Citations (0)