Ines Mihaljević

Agricultural Institute Osijek

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Vesna Tomaš

Agricultural Institute Osijek

Krunoslav Dugalić

Agricultural Institute Osijek

Marija Viljevac Vuletić

Agricultural Institute Osijek

Dominik Vuković

Agricultural Institute Osijek

Zorica Jurković

Agricultural Institute Osijek

Rezica Sudar

Agricultural Institute Osijek

Hrvoje Lepeduš

University of Osijek

DŠ

Domagoj Šimić

Agricultural Institute Osijek

Daniela Horvat

Agricultural Institute Osijek

Zvonimir Zdunić

Agricultural Institute Osijek

Cooperative Institutions

Agricultural Institute Osijek

University of Osijek

University of Zagreb

Croatian Personal Data Protection Agency

Croatian Veterinary Institute

Agricultural Institute

Institute of Agriculture and Tourism

United States Department of Agriculture

Agricultural Research Service

Maize Research Institute Zemun Polje

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Physiological Response to Short-Term Heat Stress in the Leaves of Traditional and Modern Plum (Prunus domestica L.) Cultivars

Horticulturae (2022)

Marija Viljevac Vuletić Ines Mihaljević Vesna Tomaš Daniela Horvat Zvonimir Zdunić

The aim of this study was to evaluate physiological responses to short-term heat stress in the leaves of traditional (Bistrica) and modern (Toptaste) plum cultivars. In this study, detached plum leaves were incubated at 25 °C (control) and 40 °C (stress). After 1 h of exposure to heat (40 °C), chlorophyll a fluorescence transients were measured, and several biochemical parameters were analyzed. Elevated temperature caused heat stress in both plum cultivars, seen as a decrease in water content (WT), but in the leaves of the cultivar Bistrica, an accumulation of proline and phenols, as well as an accumulation of photosynthetic pigments, suggest the activation of a significant response to unfavorable conditions. Conversely, in the leaves of Toptaste, a significant accumulation of malondialdehyde (MDA) and an activation of guaiacol peroxidase (GPOD), all together with a decreased soluble proteins content, indicate an inadequate response to maintaining homeostasis in the leaf metabolism. The impact of an elevated temperature on photosynthesis was significant in both plum cultivars as reflected in the decrease in performance indexes (PIABS and PItotal) and the maximum quantum yield of PSII (Fv/Fm), with significantly pronounced changes found in Toptaste. Unlike the traditional plum cultivar, Bistrica, in the modern cultivar, Toptaste, short-term heat stress increased the minimal fluorescence (F0) and absorption (ABS/RC), as well as Chl b in total chlorophylls. Additionally, the inactivation of RCs (RC/ABS) suggests that excitation energy was not trapped efficiently in the electron chain transport, which resulted in stronger dissipation (DI0/RC) and the formation of ROSs. Considering all presented results, it can be presumed that the traditional cultivar Bistrica has better tolerance to heat stress than the modern cultivar Toptaste. The cultivar, Bistrica, can be used as a basis in further plum breeding programs, as a source of tolerance for high temperature stress.

Malondialdehyde

10.3390/horticulturae8010072

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Antioksidacijski sustav u listovima jabuke u ekološkom i integriranom sustavu zaštite

Marija Viljevac Vuletić Vesna Tomaš Ines Mihaljević Zorica Jurković Krunoslav Dugalić

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Propagation of three Blue Honeysuckles (Lonicera caerulea L) cultivars in in vitro culture

Ines Mihaljević Vesna Tomaš Dominik Vuković Krunoslav Dugalić

Caerulea

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Chlorophylls content and photosynthetic efficiency in two sour cherry (Prunus cerasus L.) genotypes under drought stress

Acta Botanica Croatica (2013)

Marija Viljevac Vuletić Krunoslav Dugalić Ines Mihaljević Domagoj Šimić Rezica Sudar

The drought is limiting factor in fruit production today. Identification of sour cherry genotypes tolerant to drought will enable the sustainability of fruit production. The aim of our study was to select sour cherry genotypes according to their genetic background as well as drought tolerance and investigate possible mechanisms of drought tolerance through the changes in photosynthetic apparatus (i.e. photosynthetic pigments content) and photosynthesis process assessed through chlorophyll fluorescence transient. All together with molecular markers (SSRS and AFLPs), relative water content (RWC), as indicator of plant water status, sort out two genotypes (Kelleris 16 and OS) opposite in regards to drought tolerance. Down-regulation of photosynthesis in drought treated Kelleris 16 plants was seen as changes in antenna complexes of PSII (decreased total chlorophylls content ( a+b ) and chlorophylls ratio ( a/b )). Despite unchanged maximum quantum yield of PSII in drought treated leaves of genotype OS, overall photosynthetic performance expressed as PI ABS was down-regulated in both investigated genotypes. However, decrement of PI ABS were much pronounced in genotype Kelleris 16 mainly because of changes in a certain fraction of RCs which become dissipative centres, seen as increase in ABS/RC and DI 0 /RC, in order to avoid photooxidative damage of photosynthetic apparatus. Also, electron transport, seen as decrease in ET 0 /(TR 0 -ET 0 ) and ET 0 /RC, was impaired which lead to poored CO 2 fixation and photosynthesis. Described changes in functioning of photosynthetic apparatus in drought treated plants of Kelleris 16 are main distinction between two investigated genotypes regarding to drought adaptation mechanisms.

Drought Tolerance

Photosynthetic capacity

Photosynthetic pigment

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Maximum quantum yield of photosystem II and chlorophyll content in leaves of sour cherry cultivars challenged by drought

Marija Viljevac Vuletić Hrvoje Lepeduš Krunoslav Dugalić Ines Mihaljević Boris Puškar

Photosynthesis is the basic process influencing crop productivity. It can be inhibited by drought. Seven sour cherry genotypes were challenged by drought to assess their photosynthetic efficiency in such conditions. All together with relative water content (RWC) as indicator of plant water status, maximum quantum yield of photosystem II (Fv/Fm) and chlorophyll content (Chl a+b) differentiated investigated cultivars in their susceptibility to drought stress. Results suggest that type OS of sour cherry cv. Oblacinska is the most tolerant of all investigated genotypes. Also, chlorophyll a fluorescence monitoring appeared to be a tool of choice to diagnose drought stress tolerance of sour cherry genotypes.

Drought Tolerance

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Photochemical efficiency of photosystem II in two apple cultivars affected by elevated temperature and excess light in vivo

South African Journal of Botany (2020)

Ines Mihaljević Hrvoje Lepeduš Domagoj Šimić Marija Viljevac Vuletić Vesna Tomaš

In orchards, high temperature and high light stress are the most common abiotic stresses, which negatively affect fruit yield and quality. In this study, the physiological response of apple (Malus × domestica Borkh) cultivars to combination of increased temperature and excess light, which causes disorder of photosynthesis, was investigated. The PSII functionality and regulatory mechanisms in leaves of two apple cultivars in the field, under elevated temperature and excess light at midday, were evaluated. Chlorophyll a (Chl a) fluorescence transient was recorded using Plant efficiency analyzer (PEA), analyzed according to JIP test (analysis of O-J-I-P fluorescence transient) and several parameters were derived to explain the PSII behavior and photosynthetic efficiency. Tested cultivars showed different responses to investigated conditions and PCA analysis identified the cultivar Gold Rush as more tolerant with better thermostability of PSII. The lower efficiency of PSII antenna complexes in the Gala Galaxy cultivar caused higher increase in the heat dissipation value of excess absorbed light per active reaction center (DI0/RC) and a decrease in the captured photon flow rate and energy dissipation (TR0/DI0), which significantly contributes to its lower photosynthetic efficiency of performance index (PIABS) and total photosynthetic performance (PItotal). Cultivar Gold Rush had higher connectivity of light harvesting complexes to its reaction centers, less damage to OEC activity and better stability of the PS II system. The results obtained in this study may be useful in apple breeding programs, particularly for the selection of apple cultivars more tolerant and adjustable to higher temperature and irradiation.

Malus

Photoinhibition

Light intensity

10.1016/j.sajb.2020.01.017

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ANALIZA PONOVLJENIH MJERENJA ZA PROMJENU FOTOSINTETSKE UČINKOVITOSTI KOD VIŠNJE TIJEKOM VODNOGA DEFICITA

Marija Viljevac Vuletić Krunoslav Dugalić Hrvoje Lepeduš Ines Mihaljević Zorica Jurković

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Učinkovitost bioloških preparata u suzbijanju jabučnog savijača (Cydia pomonella L.) u nasadu jabuka

Vesna Tomaš Mirjana Brmež Krunoslav Dugalić Domagoj Šimić Ines Mihaljević

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Chlorophyll content, photosynthetic efficiency and genetic markers in two sour cherry (Prunus cerasus L.) genotypes under drought stress

Acta Botanica Croatica (2013)

Marija Viljevac Vuletić Krunoslav Dugalić Ines Mihaljević Domagoj Šimić Rezica Sudar

Abstract - Drought is a limiting factor in fruit production today. Identification of sour cherry genotypes tolerant to drought will enable the sustainability of fruit production. The aim of our study was to select sour cherry genotypes according to their genetic background as well as drought tolerance and investigate possible mechanisms of drought tolerance through the changes in photosynthetic apparatus (i.e. photosynthetic pigment content) and photosynthesis process assessed through the chlorophyll fluorescence transient. All of them together with molecular markers (SSRs and AFLPs), relative water content (RWC) as indicator of plant water status distinguish two genotypes (Kelleris 16 and OS), which are the opposite in regards to drought tolerance. Down-regulation of photosynthesis in drought-treated Kelleris 16 plants was seen as changes in antenna complexes of PSII (decreased total chlorophylls content (a+b) and chlorophylls ratio (a/b)). Despite unchanged maximum quantum yield of PSII in drought-treated leaves of genotype OS, overall photosynthetic performance expressed as PI ABS was down-regulated in both investigated genotypes. However, decrement of PI ABS was much pronounced in genotype Kelleris 16, mainly because of changes in a certain fraction of RCs, which become dissipative centres, seen as increase in ABS/RC and DI 0 /RC, in order to avoid photooxidative damage of photosynthetic apparatus. Also, electron transport, seen as decrease in ET 0 /(TR 0 -ET 0 ) and ET 0 /RC, was impaired which lead to impaired CO 2 fixation and photosynthesis. The described changes in the functioning of photosynthetic apparatus in drought-treated plants of Kelleris 16 constitute the main distinction between the two investigated genotypes regarding drought adaptation mechanisms.

Drought Tolerance

Photosynthetic capacity

Photosynthetic pigment

10.2478/botcro-2013-0003

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Relation between polyphenols content and skin colour in sour cherry fruits

Journal of Agricultural Sciences Belgrade (2012)

Marija Viljevac Vuletić Krunoslav Dugalić Vlatka Jurković Ines Mihaljević Vesna Tomaš

Fruit skin colour plays a major role in quality assessment of food, significantly determining consumer`s choice. Colour of sour cherries depends on anthocyanins which are phenolic compounds (flavonoids) present in high amounts in fruits. The aim of this study was to determine a possible relation between polyphenols (total phenolics and anthocyanins) and colour parameters of fruit skin of sour cherries. The plant material used in this study was twenty two sour cherry genotypes from an orchard of Agricultural Institute Osijek. Total phenolics and anthocyanins contents as well as colour parameters (L*, a*, b*, h and C) of fruit skin were determined. Variability between sour cherry genotypes in total phenolics and anthocyanins as well as in colour parameters was revealed. Total polyphenols content varied from 462.7 to 1049.0 mg GAE/100 g of fresh weight, while total anthocyanins ranged from 160.1 to 495.6 mg CGE/100 g of fresh weight. A significant positive correlation was found between polyphenols and anthocyanins content. Total phenolics content had a significant negative correlation with colour parameters b* and h, while anthocyanins content negatively correlated with colour parameters L*, b* and h. According to the obtained results, genotypes Maraska, Heimanns Konservenweichsel and Rexelle are the richest genotypes in polyphenols and anthocyanins content.

Orchard

10.2298/jas1202057v

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Citations (17)