Sadi Fadhil Al-Samarrai

The University of Tokyo

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Koji Uchizono

The University of Tokyo

Hiroshi Nishiye

Juntendo University

Hideya Ikemoto

Stanford University

K Sakakibara

The University of Tokyo

A. Takeuchi

University of Hyogo

Yukihiko Shimada

Kansai Rosai Hospital

Tsuyoshi Ozaki

Mitsubishi Electric (Japan)

Kazuaki Ohsawa

The University of Tokyo

Hisako Endo

Asahikawa Medical University

K. Nagashima

Hokkaido University

Cooperative Institutions

The University of Tokyo

National Institute for Physiological Sciences

Juntendo University

Czech Academy of Sciences, Institute of Physiology

Tohoku University

Osaka University

Nagoya University

Osaka Medical and Pharmaceutical University

National Institutes of Natural Sciences

Tokyo Metropolitan Institute of Gerontology

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A new method for scanning and transmission electron microscopy of synaptic vesicles isolated from the cerebral cortex.

Archivum histologicum japonicum (1985)

Sadi Fadhil Al-Samarrai Tsuyoshi Ozaki Hiroshi Nishiye Koji Uchizono

Spheroid and flattened synaptic vesicles were isolated from the brain homogenate of guinea pigs by a modified purification method. For scanning and transmission electron microscopy, a simple dipping method of preparation was developed and used. The purest and richest fraction of synaptic vesicles was obtained from a 0.1M sucrose fraction of density gradients. The pellets of synaptic vesicles were easily resuspended without aggregate after ultracentrifugation at 40, 000rpm for 5min. The isolated synaptic vesicles were dispersed as a monolayer on the surface of a copper grid covered with Formvar membrane. Adequate contrast was obtained by metal impregnation of specimens and gold coating at magnifications as high as 100, 000 times using an accelaration voltage of 25 to 40kV. The specimens were fixed in 0.75% glutaraldehyde (0.1M phosphate buffer, pH 7.3) and then postfixed in 1% osmium tetroxide. After dipping for 1 to 2min each in tannic acid, phosphotungstic acid, lead citrate and uranyl acetate, they were dehydrated with graded ethanol and coated with gold by ion sputtering at 400 to 560 volts for 4min. The preparation method is reported on and technical problems are discussed.

Uranyl acetate

Osmium tetroxide

Phosphotungstic acid

Glutaraldehyde

10.1679/aohc.48.109

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Electron microscopic study on the response of the normal canine brain to boron-neutron capture therapy.

PubMed (1975)

Sadi Fadhil Al-Samarrai A. Takeuchi Hiroshi Hatanaka

The response of normal cerebral tissue of dogs to boron-neutron capture therapy by the currently available improved method was studied by electron microscopy. Peripheral blood capillaries of the neutron-irradiated area in the left cerebral hemisphere were compared with their counterparts in the shielded and non-irradiated right hemisphere. No ultrastructural changes, as those noted in classical method of boron-neutron irradiation by the improved technique of boron-neutron capture therapy. There was no swelling of endothelial cells, disappearance of cristae of mitochondria, increased pinocytosis, disappearance of ribosomes, enlargement of Golgi apparatus, or increased appearance of endoplasmic reticulum. Basement membrane was not disrupted and was uniform. Pericytes, synaptosomes, and other glial elements remained intact. In contrast to the old clinical trials up to 1961, the renewed boron-neutron capture therapy is regarded not to cause serious damage to the central nervous system.

Nuclear membrane

Neutron capture

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Morphological and biochemical properties of synaptic vesicles isolated from guinea pig brain

Neuroscience (1979)

Koji Uchizono Kazuaki Ohsawa Hideya Ikemoto Sadi Fadhil Al-Samarrai

10.1016/0306-4522(79)90136-2

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Citations (9)

Scanning electron microscopical studies on human erythrocytes

Sadi Fadhil Al-Samarrai

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A NEW TYPE OF MUCOLIPIDOSIS ASSOCIATED WITH HEREDITARY THROMBOCYTOPATHY AND COLOR BLINDNESS

Acta Pathologica Japonica (1977)

Hisako Endo Sadi Fadhil Al-Samarrai K Sakakibara K. Nagashima Yukihiko Shimada

Autopsy findings of a 22‐year‐old Japanese male who showed the symptoms of both mucopolysaccharidosis and sphingolipidosis are reported. The patient had a gargoyle‐like face, bone change with cherry‐red spot and absence of mucopolysacchariduria, and moreover accompanied by hereditary thrombocytopathy and color blindness. Autopsy Andings were almost the same as those of mucopolysaccharidosis, histochemically and electron microscopically. Unique findings were, however, present; In the hepatocytes, another inclusion containing dense Ane granuloreticular structures was found electron microscopically. Some foamy cells in the lymph nodes, liver including sinusoidal cells, bone marrow and spleen contained intracytoplasmic sudanophillc substance in the form of moderate electron dense globules by electron microscopy. The outstanding finding of the enzymatic activity was the decrease of β‐galactosidase in the liver and brain.

Mucopolysaccharidosis

Inclusion bodies

Mucolipidosis

Organomegaly

10.1111/j.1440-1827.1977.tb00165.x

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Citations (11)

Studies on Preparation Technique of Human Erythrocytes for Clinical Scanning Electron Microscopy

Archivum histologicum japonicum (1975)

Sadi Fadhil Al-Samarrai

Various methods of preparation were tested and modified in order to establish a proper method of preparing normal and pathological human erythrocytes for scanning electron microscopy. Morphological changes after various preparation techniques, such as echinocytosis, spherocytosis, elliptocytosis and knizocytosis were studied and evaluated statistically. The best method, so far obtained to preserve 99% of normal erythrocytes as biconcave discocytes, was that of venous blood without anticoagulants or acid-citrate-dextrose, washed with physiological saline at 37°C, fixed in 0.75% glutaraldehyde in 0.1M phosphate buffer, pH 7.3 (318 mosmol), postfixed in 1% osmium tetroxide, dehydrated with graded ethanol and amyl acetate, dried with critical point drying method, and coated with carbon and gold.

Osmium tetroxide

Glutaraldehyde

Spherocytosis

Phosphate buffered saline

Echinocyte

10.1679/aohc1950.38.321

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Citations (3)