Brenda Cardoso

Universidade de São Paulo

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Nilton Lincopán

Universidade de São Paulo

Fernanda Esposito

Universidade de São Paulo

Herrison Fontana

Universidade de São Paulo

Bruna Fuga

Universidade de São Paulo

Fábio P. Sellera

Universidade de São Paulo

Elder Sano

Universidade de São Paulo

Quézia Moura

Instituto Federal do Espírito Santo

Danny Fuentes‐Castillo

University of Concepción

Louise Cerdeira

Liverpool School of Tropical Medicine

Adriana Cardenas-Arias

Universidade de São Paulo

Cooperative Institutions

Universidade de São Paulo

Hospital Universitário da Universidade de São Paulo

Centro Universitário de Patos de Minas

Universidade Federal de São Paulo

Universidade Brasil

Universidade Federal de Uberlândia

Hospital São Paulo

Universidade Estadual de Campinas (UNICAMP)

Instituto Butantan

Universidade da Região de Joinville

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Colistin-Resistant Enterobacter kobei carrying mcr-9.1 and blaCTX-M-15 infecting a critically endangered Franciscana dolphin (Pontoporia blainvillei), Brazil

Authorea (Authorea) (2020)

Danny Fuentes‐Castillo F bio Sellera Daphne Wrobel Goldberg Herrison Fontana Fernanda Esposito

The emergence of mobile mcr genes mediating resistance to colistin is a critical public health issue that has hindered the treatment of serious infections caused by multidrug-resistant pathogens in humans and other animals. We report the emergence of the mcr-9.1 gene in a polymyxin-resistant extended-spectrum β-lactamase (ESBL)-producing Enterobacter kobei infecting a free-living Franciscana dolphin (Pontoporia blainvillei), threatened with extinction in South America. Genomic analysis confirmed a wide resistome with additional presence of genes conferring resistance to clinically relevant β-lactam [blaCTX-M-15, blaACT-9, blaOXA-1 and blaTEM-1B], aminoglycoside [aac(3)-IIa, aadA1, aph(3’‘)-Ib and aph(6)-Id], trimethoprim [dfrA14], tetracycline [tetA], quinolone [aac(6’)-Ib-cr and qnrB1], fosfomycin [fosA], sulphonamide [sul2], and phenicol [catA1 and catB3] antibiotics. The identification of mcr-9.1 in a CTX-M-15-producing pathogen infecting a critically endangered animal is worryingly, due to the restricted therapeutic options, and should be interpreted as a sign of further spread of critical-priority pathogens and their resistance genes in threatened ecosystems.

Colistin

Resistome

Tigecycline

Fosfomycin

Polymyxin

10.22541/au.159819284.45187631

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Citations (3)

Successful expansion of hospital-associated clone of vanA-positive vancomycin-resistant Enterococcus faecalis ST9 to an anthropogenically polluted mangrove in Brazil

Marine Pollution Bulletin (2023)

Andrey G. Sacramento Bruna Fuga Herrison Fontana Brenda Cardoso Fernanda Esposito

Enterococcus faecalis

Vancomycin-resistant Enterococcus

Multilocus sequence typing

Mangrove ecosystem

10.1016/j.marpolbul.2023.115844

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Genomic features of a polymyxin-resistant Klebsiella pneumoniae ST491 isolate co-harbouring blaCTX-M-8 and qnrE1 genes from a hospitalised cat in São Paulo, Brazil

Journal of Global Antimicrobial Resistance (2020)

Luciana Sartori Fábio P. Sellera Quézia Moura Brenda Cardoso Herrison Fontana

Klebsiella pneumoniae has emerged as one of the major pathogens of humans and companion animals. Moreover, polymyxin resistance in K. pneumoniae is increasingly reported worldwide, mainly among extended-spectrum β-lactamase (ESBL)- and/or carbapenemase-producing isolates. The aim of this study was to report the draft genome sequence of a polymyxin-resistant, ESBL-producing K. pneumoniae isolate (14CSI) from a hospitalised domestic cat in Brazil.Whole-genome sequencing of strain 14CSI was performed on an Illumina NextSeq platform and the genome was de novo assembled using Velvet v.1.2.10. Data analysis was performed using bioinformatics tools available from the Center for Genomic Epidemiology and the Institut Pasteur database.The genome size of strain 14CSI was calculated at 5 260 459 bp, with a GC content of 57.3% and comprising 5294 total genes, 28 tRNAs, 7 rRNAs, 8 ncRNAs and 237 pseudogenes. Klebsiella pneumoniae strain 14CSI belongs to sequence type 491 (ST491), presents a mutation (A14S) in the mgrB gene and co-harbours blaCTX-M-8 and qnrE1 genes. Genes conferring resistance to heavy metals were further identified.This draft genome could be used as a reference sequence for comparative analysis of polymyxin-resistant and/or CTX-M-8-producing K. pneumoniae strains circulating at the human-animal interface.

Pseudogene

GC-content

Polymyxin

Strain (injury)

10.1016/j.jgar.2020.03.006

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One health clones of multidrug-resistant Escherichia coli carried by synanthropic animals in Brazil

One Health (2022)

Elder Sano Fernanda Esposito Herrison Fontana Bruna Fuga Adriana Cardenas-Arias

WHO priority pathogens have disseminated beyond hospital settings and are now being detected in urban and wild animals worldwide. In this regard, synanthropic animals such as urban pigeons (Columba livia) and rodents (Rattus rattus, Rattus norvegicus and Mus musculus) are of interest to public health due to their role as reservoirs of pathogens that can cause severe diseases. These animals usually live in highly contaminated environments and have frequent interactions with humans, domestic animals, and food chain, becoming sentinels of anthropogenic activities. In this study, we report genomic data of Escherichia coli strains selected for ceftriaxone and ciprofloxacin resistance, isolated from pigeons and black rats. Genomic analysis revealed the occurrence of international clones belonging to ST10, ST155, ST224 and ST457, carrying a broad resistome to beta-lactams, aminoglycosides, trimethoprim/sulfamethoxazole, fluoroquinolones, tetracyclines and/or phenicols. SNP-based phylogenomic investigation confirmed clonal relatedness with high-risk lineages circulating at the human-animal-environmental interface globally. Our results confirm the dissemination of WHO priority CTX-M-positive E. coli in urban rodents and pigeons in Brazil, highlighting potential of these animals as infection sources and hotspot for dissemination of clinically relevant pathogens and their resistance genes, which is a critical issue within a One Health perspective.

Resistome

Disease reservoir

10.1016/j.onehlt.2022.100476

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Citations (11)

Human pandemic K27-ST392 CTX-M-15 extended-spectrum β-lactamase-positive Klebsiella pneumoniae: A one health clone threatening companion animals

One Health (2022)

Luciano C.B.A. da Silva Brenda Cardoso Herrison Fontana Fernanda Esposito Srg Cortopassi

Extended spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae is a medically important pathogen that commonly causes human nosocomial infections. Since veterinary emergency and critical care services have also significantly progressed over the last decades, there are increasing reports of ESBL-producing K. pneumoniae causing hospital-associated infections in companion animals. We present microbiological and genomic analysis of a multidrug-resistant ESBL-positive K. pneumoniae (LCKp01) isolated from a fatal infection in a dog admitted to a veterinary intensive care unit. LCKp01 strain belonged to the sequence type ST392 and displays a KL27 (wzi-187) and O-locus 4 (O4). A broad resistome and presence of the blaCTX-M-15 ESBL gene were predicted. SNP-based phylogenomic analysis, using an international genome database, clustered LCKp01 (60-80 SNPs differences) with K. pneumoniae ST392 from human and animal infections, isolated at 4-year interval, whereas phylogeographical analysis confirmed successful expansion of ST392 as a global clone of One Health concern.

Pandemic

Resistome

clone (Java method)

10.1016/j.onehlt.2022.100414

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Genomic Analysis of a Highly Virulent NDM-1-Producing Escherichia coli ST162 Infecting a Pygmy Sperm Whale (Kogia breviceps) in South America

Frontiers in Microbiology (2022)

Fábio P. Sellera Brenda Cardoso Danny Fuentes‐Castillo Fernanda Esposito Elder Sano

Carbapenemase-producing Enterobacterales are rapidly spreading and adapting to different environments beyond hospital settings. During COVID-19 lockdown, a carbapenem-resistant NDM-1-positive Escherichia coli isolate (BA01 strain) was recovered from a pygmy sperm whale (Kogia breviceps), which was found stranded on the southern coast of Brazil. BA01 strain belonged to the global sequence type (ST) 162 and carried the blaNDM-1, besides other medically important antimicrobial resistance genes. Additionally, genes associated with resistance to heavy metals, biocides, and glyphosate were also detected. Halophilic behavior (tolerance to > 10% NaCl) of BA01 strain was confirmed by tolerance tests of NaCl minimal inhibitory concentration, whereas halotolerance associated genes katE and nhaA, which encodes for catalase and Na+/H+ antiporter cytoplasmic membrane, respectively, were in silico confirmed. Phylogenomics clustered BA01 with poultry- and human-associated ST162 lineages circulating in European and Asian countries. Important virulence genes, including the astA (a gene encoding an enterotoxin associated with human and animal infections) were detected, whereas in vivo experiments using the Galleria mellonella infection model confirmed the virulent behavior of the BA01 strain. WHO critical priority carbapenemase-producing pathogens in coastal water are an emerging threat that deserves the urgent need to assess the role of the aquatic environment in its global epidemiology.

Galleria mellonella

10.3389/fmicb.2022.915375

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Citations (11)

WHO Critical Priority Escherichia coli as One Health Challenge for a Post-Pandemic Scenario: Genomic Surveillance and Analysis of Current Trends in Brazil

Microbiology Spectrum (2022)

Bruna Fuga Fábio P. Sellera Louise Cerdeira Fernanda Esposito Brenda Cardoso

A One Health approach for antimicrobial resistance must integrate whole-genome sequencing surveillance data of critical priority pathogens from human, animal and environmental sources to track hot spots and routes of transmission and developing effective prevention and control strategies. As part of the Grand Challenges Explorations: New Approaches to Characterize the Global Burden of Antimicrobial Resistance Program, we present genomic data of WHO critical priority carbapenemase-resistant, ESBL-producing, and/or colistin-resistant Escherichia coli strains isolated from humans and nonhuman sources in Brazil, a country with continental proportions and high levels of antimicrobial resistance.

Resistome

Colistin

10.1128/spectrum.01256-21

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Citations (62)

AS CONSEQUÊNCIAS DA INFECÇÃO DO COVID-19 PARA PACIENTES CARDIOPATAS

Thaís Allemagne Carvalho Vilarinho Gabriela Luiza Nogueira Camargos Stefeny Beatriz Bonfim Duarte Camila Mendes Silva Alanna Simão Gomes

10.22533/at.ed.1252305107

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Genomic characterization of multidrug-resistant Salmonella Heidelberg E2 strain isolated from chicken carcass in southern Brazil

International Journal of Food Microbiology (2022)