Angie Ambers

Autosomal STR typing results for 140-year-old skeletal remains from Deadwood, Dakota using the Illumina® ForenSeq™ DNA Signature Prep Kit and MiSeq® MPS platform (E1 = elution #1; E2 = elution #2). (XLSX 12 kb)

Massive parallel sequencing

Identification

Forensic Genetics

Forensic identification

10.6084/m9.figshare.c.3604163_d6

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Comparison of standard capillary electrophoresis based genotyping method and ForenSeq DNA Signature Prep kit (Illumina) on a set of challenging samples

Forensic science international. Genetics supplement series (2017)

Jitka Votrubová Angie Ambers Bruce Budowle Daniel Vaněk

genomic DNA

10.1016/j.fsigss.2017.09.027

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Citations (4)

DNA extraction methods for human skeletal remains

Elsevier eBooks (2023)

Angie Ambers

Geneticist

10.1016/b978-0-12-815766-4.00006-6

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Citations (1)

Missing persons and unidentified human remains: The world’s silent mass disaster

Elsevier eBooks (2023)

Angie Ambers

10.1016/b978-0-12-815766-4.00001-7

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Investigation of strategies for improving str typing of degraded and low copy DNA from human skeletal remains and bloodstains

Angie Ambers

Forensic STR analysis is limited by the quality and quantity of DNA. Significant damage or alteration to the molecular structure of DNA by depurination, crosslinking, base modification, and strand breakage can impact typing success. Two methods that could potentially improve STR typing of challenged samples were explored: an in vitro DNA repair assay (PreCR™ Repair Mix) and whole genome amplification. Results with the repair assay showed trends of improved performance of STR profiling of bleach-damaged DNA. However, the repair assay did not improve DNA profiles from environmentally-damaged bloodstains or bone, and in some cases resulted in lower RFU values for STR alleles. The extensive spectrum of DNA damage and myriad combinations of lesions that can be present in forensic samples appears to pose a challenge for the in vitro PreCR™ assay. The data suggest that the use of PreCR™ in casework should be considered with caution due to the assay’s varied results. As an alternative to repair, whole genome amplification (WGA) was pursued. The DOP-PCR method was selected for WGA because of initial primer design and greater efficacy for amplifying degraded samples. Several modifications of the original DOP-PCR primer were evaluated. These modifications allowed for an overall more robust amplification of damaged DNA from both contemporary and historical skeletal remains compared with that obtained by standard DNA typing and a previously described DOP-PCR method. These new DOP-PCR primers show promise for WGA of degraded DNA.

Primer (cosmetics)

Multiple displacement amplification

STR analysis

Low copy number

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Additional file 1: of More comprehensive forensic genetic marker analyses for accurate human remains identification using massively parallel DNA sequencing

Figshare (2016)

Angie Ambers Jennifer D. Churchill Jonathan L. King Monika Stoljarova Harrell Gill-King

Y-SNP data for three different bone powder fractions using the HID-Ion AmpliSeq™ Identity Panel and the Ion Torrent PGM® MPS platform (E1 = elution #1; E2 = elution #2). (XLSX 12 kb)

Massive parallel sequencing

Identification

10.6084/m9.figshare.c.3604163_d2

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Additional file 4: of More comprehensive forensic genetic marker analyses for accurate human remains identification using massively parallel DNA sequencing

Figshare (2016)

Angie Ambers Jennifer D. Churchill Jonathan L. King Monika Stoljarova Harrell Gill-King

Phenotype-informative SNP analysis results for 140-year-old skeletal remains from Deadwood, Dakota using the: A) Illumina® ForenSeq™ DNA Signature Prep Kit and B) HID-Ion AmpliSeq™ Externally Visible Characteristics (EVC) Prototype Panel (E1 = elution #1; E2 = elution #2). Concordant results were used to generate a composite profile (C). (XLSX 12 kb)

Massive parallel sequencing

Identification

10.6084/m9.figshare.c.3604163_d5

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Forensic genetic investigation of human skeletal remains recovered from the La Belle shipwreck

Forensic Science International (2019)

Angie Ambers Magdalena M. Buś Jonathan L. King Bradford M. Jones Jeffrey J. Durst

Forensic anthropology

10.1016/j.forsciint.2019.110050

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Citations (25)