Smilax glabra Roxb (SGR) as a novel anti-tumor agent has been paid attention in several types of cancer cells. However, the effect of SGR on SGC7901 cells has not been investigated.We investigate the effect and potential mechanisms of SGR on SGC7901 cells in this study.Three kinds of gastric cancer cell lines (BGC823, SGC7901 and MKN45) and one kind of human embryonic kidney cell line (HEK293) were exposed to varying concentrations of SRG. Then, we observed the effect of SRG on these cell lines and the changes on proliferation, invasion and apoptosis. Finally, we detected the signaling pathway in which SGR may involve.SGR effectively suppressed the proliferation of SGC7901 cell lines by inhibiting the phosphorylation of Akt (Thr308). Moreover, we found SGR could significantly induce SGC7901 cell lines apoptosis by inhibiting Akt(p-Thr308)/Bad pathway and inhibit its migration and invasion partly by inhibiting Akt(p-Thr308)/MMPs pathway.SGR could effectively suppress the proliferation and invasion of SGC7901 cell lines by inhibiting the phosphorylation of Akt (Thr308) and its downstream relative pathways.SGR could effectively suppress the phosphorylation of Akt (Thr308) and then inhibit the proliferation and invasion of SGC7901 cell and enhance its apoptosis through Akt-mediated signaling pathways.
Objective: To analyze the characteristics of pneumoconiosis patients and the basic status of medical treatment. Methods: Research objects were chosen by stratified sampling method and typical survey method from existing pneumoconiosis patients in China. The survey was carried out from March 2017 to January 2018 in nine provinces including provinces from east, medium and western region in China. Source of pneumoconiosis cases were inpatient cases, outpatient or physical-examined cases and household-investigation cases. The survey mainly included demographic and sociological characteristics, economic status, occupational history and dust exposure history, disease status, work-related injury insurance and social security status and related indicators of pneumoconiosis treatment. Results: Investigated 1037 pneumoconiosis cases which included 186 (19.9%) household-investigation cases, 212 (20.4%) outpatient or physical-examined cases and 639 (61.7%) inpatient cases. Demographic and sociological characteristics, individual monthly income, economic source, occupational history and work-related injury insurance were statistically significant among different source of pneumoconiosis patients (P<0.05) . Among all of the household-investigation cases, there were 74 cases (40.2%) had no income, 117 cases (62.9%) used to work in private enterprises, 36 cases (19.4%) had work-related injuries insurance, 95 cases (51.1%) were at three phase of pneumoconiosis, 108 cases (59.0%) haven't had any drugs for pneumoconiosis. 65 cases (39.4%) haven't went to the clinic, 53 cases (28.5%) hadn't seek medical advice although they needed medical treatment very much. Among all of the outpatient or physical-examined cases, there were 95 cases (46.1%) had no income, 36 cases (17.0%) had work-related injuries Insurance, 139 cases (65.6%) went to the clinic for treatment of pneumoconiosis, 81 cases (38.2%) went to the clinic for more than ten times. Among all the inpatient cases, 310 cases' (49.3%) personal monthly income was above 2000 yuan, 352 cases (55.1%) had work-related injuries Insurance, 588 cases (92.2%) were taking drugs for treatment of pneumoconiosis, 153 canses (24.2%) had hospitalization for than ten times. Conclusion: Household-investigation cases have lower economic conditions, lower rates of Insurance coverage for work-related injuries, severer pneumoconiosis and higher clinical service utilization. Clinical or physical-examined cases have lower economic conditions, lower rates of Insurance coverage for work-related injuries and higher clinical service utilization. Hospitalized cases have better economic conditions, higher rates of insurance coverage for work-related injuries and higher hospitalization service utilization.目的： 调查我国尘肺病患者的基本特征及就医现状。 方法： 于2017年3月至2018年1月，在浙江省、江苏省、山东省等9个省采取分层抽样调查和典型调查相结合的方法，开展问卷调查，尘肺病例来源分别为入户调查病例、门诊或体检筛查病例和住院病例，主要调查尘肺病患者的人口社会学特征、经济状况、职业史、接尘史、患病状况、工伤保险和社会保障情况等内容。 结果： 共调查1 037例尘肺病患者，其中入户调查病例186例（17.9%），门诊或体检筛查病例212例（20.4%），住院病例639例（61.7%）。不同方式调查尘肺病患者的人口社会学特征、个人月收入、经济来源、职业史及工伤保险等分布差异均有统计学意义（P<0.05）。入户调查病例中无收入患者74例（40.2%），在私营企业工作117例（62.9%），工伤保险参保36例（19.4%），叁期尘肺患者95例（51.1%），调查时未使用任何尘肺病治疗药物108例（59.0%），未去过门诊就医65例（39.4%），应住院未住院53例（28.5%）。门诊或体检筛查病例中无收入患者95例（46.1%），工伤保险参保36例（17.0%），通过门诊就医治疗尘肺病139例（65.6%），门诊次数≥10次81例（38.2%）。住院病例个人月收入≥2 000元310例（49.3%），工伤保险参保352例（55.1%），调查时正在使用尘肺病治疗药物588例（92.2%），住院>10次153例（24.2%）。 结论： 尘肺病入户调查病例经济状况差，工伤保险参保比例低，尘肺病病情较严重，医疗服务利用不足；门诊或体检筛查病例经济状况差，工伤保险参保比例低，门诊服务利用率高；住院病例整体经济状况较好，工伤保险参保比例高，住院服务利用率较高。.
MicroRNAs can regulate gene expression at the posttranscriptional level through translational repression or target degradation. Our previous investigations examined the differential expression levels of chi-miR-3031 in caprine mammary gland tissues in colostrum and common milk stages.The present study detected the role of chi-miR-3031 in the lactation mechanisms of GMECs. High-throughput sequencing was used to analyze transcriptomic landscapes of GMECs transfected with chi-miR-3031 mimics (MC) and a mimic negative control (NC). In the MC and NC groups, we acquired 39,793,503 and 36,531,517 uniquely mapped reads, respectively, accounting for 85.85 and 81.66% of total reads. In the MC group, 180 differentially expressed unigenes were downregulated, whereas 157 unigenes were upregulated. KEGG pathway analyses showed that the prolactin, TNF and ErbB signaling pathways, including TGFα, PIK3R3, IGF2, ELF5, IGFBP5 and LHβ genes, played important roles in mammary development and milk secretion. Results from transcriptome sequencing, real-time PCR and western blotting showed that chi-miR-3031 suppressed the expression of IGFBP5 mRNA and protein. The expression levels of β-casein significantly increased in the MC and siRNA-IGFBP5 groups. We observed that the down-regulation of IGFBP5 activated mTOR at the Ser2448 site in GMECs transfected with MC and siRNA-IGFBP5. Previous findings and our results showed that chi-miR-3031 activated the PI3K-AKT-mTOR pathway and increased β-casein expression by down-regulating IGFBP5.These findings will afford valuable information for improving milk quality and contribute the development of potential methods for amending lactation performance.
Abstract To explore the pathogenesis of high myopia (HM) using quantitative proteomics. The aqueous humor of patients with simple nuclear cataract and nuclear cataract complicated with HM (hereinafter referred to as “C” and “HM” groups, respectively) were collected. The isobaric tags for relative and absolute quantitation (iTRAQ)-based liquid chromatography–tandem mass spectrometry (LC–MS/MS) proteomics approach was employed to explore differentially expressed proteins (DEPs). Bioinformatics was used to interpret the proteomic results. Furthermore, the plasminogen (PLG) protein was confirmed by enzyme-linked immunosorbent assay (ELISA) as the candidate biomarker for HM through a receiver operating characteristic curve analysis. The study showed 32 upregulated and 26 downregulated proteins. The gene ontology analysis demonstrated that 58 DEPs corresponded to 325 biological processes, 33 cell components, and 45 molecular functional annotations. The Kyoto Encyclopedia of Genes and Genomes analysis showed that the upregulated DEPs were highly enriched in the coagulation and complement cascades, consistent with the gene set enrichment analysis. Our data suggested that some DEPs might be hallmarks of the development of HM. ELISA confirmed that the PLG expression levels were significantly upregulated in HM. This was a new study investigating alterations in protein levels and affected pathways in HM using iTRAQ-based quantitative proteomics. Our study provided a comprehensive dataset on overall protein changes and shed light on its potential molecular mechanism in human HM.
Background: The COVID-19 pandemic poses a heightened risk of infection and adverse outcomes for cancer patients. Although COVID-19 vaccines are recommended for patients with cancer, their safety and potential impact on Epstein-Barr virus (EBV) reactivation in nasopharyngeal carcinoma (NPC) patients remain uncertain. This study aims to analyze real-world data to investigate any changes in EBV DNA copy numbers associated with COVID-19 vaccination in this patient population. Materials and Methods: From January 1, 2020 to September 1, 2022, 3658 NPC patients vaccinated prior to treatment and 3658 NPC patients unvaccinated matched by the propensity score matching method were defined as vaccinated group and unvaccinated group respectively. We compared the EBV DNA copy numbers and EBV clearance between the two treatment groups. Additionally, we performed a longitudinal analysis to assess the changes in EBV levels before and after vaccination.Results: The median EBV-DNA copy number in the vaccinated group was significantly lower than that in the unvaccinated group (340 vs. 480 copies/mL, p<0.001). In the vaccinated group, 17.7% (647/3658) had a pretreatment EBV DNA copy number ≥4000, compared to 22.1% (810/3658) in the unvaccinated group (p<0.001). At 6 months, the clearance rates were 93.9% in the vaccinated group and 92.9% in the unvaccinated group (p<0.05). Among 1968 post-treatment patients with EBV DNA undetectable before vaccination, 1964 individuals maintained undetectable after vaccination.Conclusions: COVID-19 vaccination in NPC patients does not induce EBV reactivation or result in an increase in plasma EBV copy numbers. Furthermore, there is a potential reduction in plasma EBV DNA copy numbers following vaccination.
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