Elise Bordet

Virologie et Immunologie Moléculaires

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Nicolas Bertho

Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

Edwige Bouguyon

Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

Céline Urien

Virologie et Immunologie Moléculaires

Isabelle Schwartz-Cornil

Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

Céline Barc

Centre Val de Loire

Fany Blanc

Université Paris-Saclay

Olivier Boulesteix

Infectiologie Animale et Santé Publique

Virginie Jakob

Institut de Recherche Vaccinale

Cindy Bernelin‐Cottet

NeuroDiderot

Elisa Crisci

North Carolina State University

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Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

Université Paris-Saclay

Virologie et Immunologie Moléculaires

Inserm

Institut National de la Recherche Agronomique

Commissariat à l'Énergie Atomique et aux Énergies Alternatives

Centre National de la Recherche Scientifique

Université de Versailles Saint-Quentin-en-Yvelines

AgroParisTech

Génétique Animale et Biologie Intégrative

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Distinctive Cellular and Metabolic Reprogramming in Porcine Lung Mononuclear Phagocytes Infected With Type 1 PRRSV Strains

Frontiers in Immunology (2020)

Elisa Crisci Marco Moroldo Thien‐Phong Vu Manh Ammara Mohammad Laurent Jourdren

Porcine reproductive and respiratory syndrome (PRRS) has an extensive impact on pig production. The causative virus (PRRSV) is divided into two species, PRRSV-1 (European origin) and PRRSV-2 (North American origin). Within PRRSV-1, PRRSV-1.3 strains, such as Lena, are more pathogenic than PRRSV-1.1 strains, such as Flanders 13 (FL13). To date, the molecular interactions of PRRSV with primary lung mononuclear phagocyte (MNP) subtypes, including conventional dendritic cells types 1 (cDC1) and 2 (cDC2), monocyte-derived DCs (moDC), and pulmonary intravascular macrophages (PIM), have not been thoroughly investigated. Here, we analyze the transcriptome profiles of in vivo FL13-infected parenchymal MNP subpopulations and of in vitro FL13- and Lena-infected parenchymal MNP. The cell-specific expression profiles of in vivo sorted cells correlated with their murine counterparts (AM, cDC1, cDC2, moDC) with the exception of PIM. Both in vivo and in vitro , FL13 infection altered the expression of a low number of host genes, and in vitro infection with Lena confirmed the higher ability of this strain to modulate host response. Machine learning (ML) and gene set enrichment analysis (GSEA) unraveled additional relevant genes and pathways modulated by FL13 infection that were not identified by conventional analyses. GSEA increased the cellular pathways enriched in the FL13 data set, but ML allowed a more complete comprehension of functional profiles during FL13 in vitro infection. Data indicates that cellular reprogramming differs upon Lena and FL13 infection and that the latter might keep antiviral and inflammatory macrophage/DC functions silent. Although the slow replication kinetics of FL13 likely contribute to differences in cellular gene expression, the data suggest distinct mechanisms of interaction of the two viruses with the innate immune system during early infection.

Reprogramming

10.3389/fimmu.2020.588411

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Citations (8)

Innate and adaptive immune responses against Porcine Reproductive and Respiratory Syndrome virus (PRRSV)

HAL (Le Centre pour la Communication Scientifique Directe) (2018)

Elise Bordet

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Immunogenicity of DNA vaccines delivered by patches and electroporation in pig skin

HAL (Le Centre pour la Communication Scientifique Directe) (2018)

Cindy Bernelin‐Cottet Céline Urien Fany Blanc Jean Jacques J. J. Leplat Joanne McCaffrey

Pig skin

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