K. Nobuto

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Takuma HANAKI

Ministry of Agriculture, Forestry and Fisheries

Usaburo SATO

National Center For Child Health and Development

Kotaro Suzuki

Kobe University

Susumu Ishii

Waseda University

Akio Kobayashi

Kumamoto University

Yukinori Tsunematsu

The University of Tokyo

Kiyoshi Imaizumi

Kanagawa Children's Medical Center

K Kamei

Teikyo Heisei University

Shunsuke Ishii

National Hospital Organization

Yutaka Nishimura

Saiseikai Suita Hospital

Cooperative Institutions

The University of Tokyo

Osaka University

National Institute of Infectious Diseases

National Institute of Health

Kumamoto University

Tokyo University of Science

National Institute of Animal Health

Teikyo University

Oita University

Hokkaido University

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Attenuation of hog cholera virus by means of continuous cell-virus propagation (CCVP) method

Archives of Virology (1964)

Usaburo SATO Yutaka Nishimura Takuma HANAKI K. Nobuto

Cholera

Medical microbiology

10.1007/bf01555832

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Citations (16)

Studies on toxoplasmosis in domestic animals. Serological response of cattle, goats and pigs to experimental infection arid successful application of the complement fixation inhibition test to detect infected herds.

K. Nobuto Kotaro Suzuki Mamoru Omuro Shunsuke Ishii

Complement fixation test

Toxoplasmosis

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Citations (1)

PREPARATION OF THE CONCENTRATED SKIN-TEST ANTIGEN (TSC) FOR SWINE TOXOPLASMOSIS

The Japanese Journal of Veterinary Science (1962)

K. Nobuto Usaburo SATO Takuma HANAKI

Toxoplasmosis

10.1292/jvms1939.24.297

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Studies on Toxoplasmosis in Domestic Animals : I. Distribution of Antibodies among Domestic Animals in Japan (Proceedings of the 45th Meeting of the Japanese Society of Veterinary Science)

The Japanese Journal of Veterinary Science (1958)

Mamoru Omura Kotaro Suzuki K. Nobuto T. Omori Shunsuke Ishii

Toxoplasmosis

Domestic animal

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RELIABILITY OF THE DYE AND MODIFIED HEMAGGLUTINATION TESTS FOR THE LATENT INFECTION OF TOXOPLASMA

The Japanese Journal of Veterinary Science (1972)

Yasuji KATSUBE Toshikatsu Hagiwara Kiyoshi Imaizumi Takuma HANAKI K. Nobuto

トキソプラズマ不顕性感染に対するSabin-Fcldmanの色素試験(DT)および花木ら(1964,1969)2~3)の血球凝集反応(HAtest)の信頼性を明らかにする目的で,人,犬,猫および豚について両血清反応を行ない,トキソプラズマの分離成績と比較検討した.これらの血清反応のうち,DTは原虫分離成績と良く一致し,不顕性感染の診断に最も信頼性が高く,ついでHAtcst-2(血清>,HAtest-1(枦紙法>の順であった.花木らは′256倍陽性をHAtcst-1における陽性限界と定めている.しかしながら,得られた成績よりすると,不顕性感染例をもれなく摘発するためには,HAtest-1,HAtcst一2ともに,64倍陽性を意義ある抗体価とみなすべきものと考えられた.動物種との関係をみると,人および犬では,DT陰性のものから本原虫が検出されることはなかったが,陽性のものからの検出率は低く,人で12例中2例,犬で24例中11例から,本原虫が分離されるとどまった.これに対し,DT陽性豚の全例(4/4)および陽性猫のほぼ全例(25/27)より本原虫が分離された.また,本原虫の分離された猫および豚の各1例は,DT陰性であった.HAtestは猫および豚の不顕性感染診断に実用性を有するものと認められ,HAtest陽性猫のほぼ全例,豚の57(HAtest-1)~30%(HAtcst-2)から本原虫が分離された.人および犬についてのHAtestは,さらに検討を要するもの考えられた.

Hemagglutination tests

10.1292/jvms1939.34.123

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Citations (9)

Relationship between Virus Content and Immunogenicity for Ferrets of Chick-Embryo-Adapted Living Distemper Vaccine

Journal of the Japan Veterinary Medical Association (1961)

Noboru FUJIE K. Kurata M. Sawada K. Nobuto

Commercial specimens of desiccated chick-embryo-adapted living distemper vaccine were examined for virus content and divided into three classes, A, B and C. The LD50 values were 2.27 to 2.43 in class A vaccine, 1.25 to 1.58 in class Bvaccine, and less than zero in class C vaccine. These samples were injected into ferrets.As a result, class A vaccine conferred power of defense against infection to the injected animals. Of six animals inoculated with class B vaccine, one died from infection. No protective power was given to animals injected with class C vaccine.The power of producing neutralizing antibodies conferred to inoculated animals was in proportion to the virus content of the vaccine inoculated. Two specimens of vaccine prepared by different commercial laboratories had different power of producing such antibodies. No difference was observed, however, in the power of neutralizing different strains of virus among the vaccine specimens studied.

Duck embryo vaccine

Canine distemper

Attenuated vaccine

10.12935/jvma1951.14.196

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COMPARISON OF THE DYE TEST AND HEMAGGLUTINATION TESTS BY THREE DIFFERENT TECHNIQUES IN THE SEROLOGIC DIAGNOSIS OF TOXOPLASMOSIS:RESULTS ON SERA FROM HUMANS, DOGS AND CATS

Japanese Journal of Infectious Diseases (1971)

Akio Kobayashi M. Kumada Yukinori Tsunematsu K Kamei K. Nobuto

Toxoplasmosis

Hemagglutination tests

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Determination of Minimum Infectious Doses of Toxoplasma in Swine by Intranasal and Intraperitoneal Routes and Hematological Findings of Infected Swine

Journal of the Japan Veterinary Medical Association (1961)

Usaburo SATO Takuma HANAKI Y. Nishimura K. Nobuto

Intranasal inoculation with 15-100 cells of the proliferative form of toxoplasma (Tp) caused no clinical infection in piglets about two months old. In inoculated piglets, no rise in dye-test titer was observed. No positive T.S.C. skin test was given by any one of them. Infection occurred when more than 1, 000 cells of Tp had been inoculated. These results seemed to indicate that the intranasal route was useful for infection.After intraperitoneal inoculation with 200 to 2, 000 cells of Tp, a rise in dye-test titer was observed and a positive T.S.C. skin test was revealed, although no inoculated piglets showed any clinical signs. It was considered that these piglets were suffering from the subclinical infection. Those piglets which had been given more than 20, 000 cells of Tp were infected successfully with clinical symptoms, giving positive dye tests and T. S.C. skin tests.In swine artificially infected, leukocytes increased in number, neutrophils increased in percentage with shift to the left of nuclei, and decreased in number but no change was seen in the number of reticulocytes. These hematological findings were considered to have a very important significance for the differential diagnosis between hog cholera and acute toxoplasmosis of swine.

Subclinical infection

Toxoplasmosis

10.12935/jvma1951.14.518

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STUDlES ON THE TISSUE CULTURE OF HOG CHOLERA VIRUS : II. NEUTRALIZATION TEST BY MEANS OF THE INFLUENCE OF HOG CHOLERA VIRUS INFECTION ON NEWCASTLE DISEASE VIRUS INFECTION (HEIC METHOD)

The Japanese Journal of Veterinary Science (1964)

Yutaka Nishimura Usaburo SATO Takuma HANAKI K. Nobuto

第I報において, われわれは, 豚腎臓組織培養細胞上で豚コレラウイルス(HCV)を培養し, ニ次ウイルスとしてニューカッスルウイルス(NDV)佐藤株を接種すると, HCV の存在において NDV による細胞変性作用(CPE)が抑制されるが, その反面, NDV の HA 価が増強する現象を利用して, HCV の証明ならびに中和抗体の測定を行なった. 今回は, さらにこの方法の一部を改良することによって, 組織培養法による HCV の証明および中和抗体の測定を一層再現性の高いものとすることができたので, この方法を HEIC 法と命名した. 1) HCV 培養期間を9日に延長し, 佐藤株培養液から牛血清を除くことによって, 旧法より HCV 証明感度が著しく上昇した. 2) 中和試験の感作時問を, 1時間(旧法)から24時間(新法)に延長することによって, クリスタル・バイオレット予防液(CVV) 5ml注射豚の中和指数を常に測定することができた(ウイルス希釈法で0.7~>1.2). また CVV 力価試験耐過豚では, 中和指数は, 血清希釈法で3.0~>5.0の値を得た. この中和試験法は, 繰り返し実験した結果, 再現性に富むもので, その値は END 法とおおむね同様であることがわかった. 3) CVV 力価試験において, 攻撃時の中和指数と攻撃反応との関係を39例について調べた. その結果, 概して中和指数が高いものは, 攻撃後軽反応で耐過し, 中和指数が低いものは, 中, 重反応または斃死を示した. ワクチン注射豚の攻撃前における中和指数は, ワクチン力価の判定の参考とすることができるものと思われる. 4) HCV 弱毒株の生毒注射によって, 中和抗体は著しく上昇し, その値は, 注射量とおおむね平行することが認められた.

Newcastle Disease

Cholera

10.1292/jvms1939.26.133

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Citations (14)

The role of pig Ascaris in the transmission of toxoplasmosis in pigs.

Kiseichūgaku zasshi (1969)

Ichiro Nakayama Tomohiro Aoki Takuma HANAKI K. Nobuto

Toxoplasmosis

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Citations (1)