Multiple chromosome 17 loci may be involved in ovarian carcinogenesis. Fifty-seven sporadic ovarian epithelial tumors were examined for loss of heterozygosity at 15 loci on chromosomes 17p. Eighty % (39 of 49) of informative tumors had allelic loss in 17p13.3 at D17S30, D17S28, or both loci within this region, including 3 of 7 tumors of low malignant potential and 4 of 5 nonmetastatic carcinomas. The smallest region of overlapping deletions extends from D17S28 to D17S30, a distance of 15 kb. Furthermore, several tumors have breakpoints within the region detected by the D17S30 probe. Chromosome 17p13.3 genes with potential tumor suppressor function include HIC-1, DPH2L (N. J. Phillips et al. Isolation of a human diphthamide biosynthesis gene on chromosome 17p13.3, submitted for publication)/OVCA1, PEDF, and CRK. The HIC-1 coding sequence lies i kb centromeric to the D17S28-S17S30 region of deletion (M. Makos Wales et al., Nat. Med., 1:570-577, 1995) but remains a candidate because 5'-regulatory elements may lie within the critical region. Portions of the DPH2L/OVCA1 coding sequence lie within the D17S28-D17S30 interval. Somatic cell hybrid analysis places PEDF in an interval including D17S28, D17S30, and D17S54, whereas CRK is excluded from this interval. Chromosome 17p13.3 loss precedes TP53 and BRCA1 region deletions because the latter changes are see only in high-stage carcinomas. Microsatellite instability plays only a minor role in sporadic ovarian carcinogenesis because only 1 of 57 tumors showed this finding.
Journal Article A new RFLP locus D4S185 maps to human chromosome 4q Get access T.C. Lairmore, T.C. Lairmore 1Departments of Surgery, Washington University Medical SchoolBox 8232, 660 S. Euclid, St Louis, MO 63110, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar A.Vocero Villeta, A.Vocero Villeta 2Departments of Genetics, Washington University Medical SchoolBox 8232, 660 S. Euclid, St Louis, MO 63110, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar S. Dou, S. Dou 2Departments of Genetics, Washington University Medical SchoolBox 8232, 660 S. Euclid, St Louis, MO 63110, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar T. Steinbrueck, T. Steinbrueck 2Departments of Genetics, Washington University Medical SchoolBox 8232, 660 S. Euclid, St Louis, MO 63110, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar H. Donis-Keller H. Donis-Keller 2Departments of Genetics, Washington University Medical SchoolBox 8232, 660 S. Euclid, St Louis, MO 63110, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 19, Issue 9, 11 May 1991, Page 2518, https://doi.org/10.1093/nar/19.9.2518 Published: 11 May 1991
Journal Article Tetranucleotide repeat polymorphism at the human thyroid peroxidase (hTPO) locus Get access R. Anker, R. Anker Department of Genetics, Washington University School of MedicineSt Louis, MO 63110, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar T. Steinbrueck, T. Steinbrueck Department of Genetics, Washington University School of MedicineSt Louis, MO 63110, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar H. Donis-Keller H. Donis-Keller * Department of Genetics, Washington University School of MedicineSt Louis, MO 63110, USA *To whom correspondence should be addressed Search for other works by this author on: Oxford Academic PubMed Google Scholar Human Molecular Genetics, Volume 1, Issue 2, May 1992, Page 137, https://doi.org/10.1093/hmg/1.2.137 Published: 01 May 1992
Journal Article A new RFLP marker D5S348 maps to 5p14.3-15.2, between D5S60 (CRrR535) and HPRTP2 Get access J.R. Howe, J.R. Howe Search for other works by this author on: Oxford Academic PubMed Google Scholar T.C. Lairmore, T.C. Lairmore Search for other works by this author on: Oxford Academic PubMed Google Scholar S. Dou, S. Dou 1Genetics, St. LouisMO 63110, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar R. Veile, R. Veile 1Genetics, St. LouisMO 63110, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar T. Steinbrueck, T. Steinbrueck 1Genetics, St. LouisMO 63110, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar S.A. Wells, Jr, S.A. Wells, Jr Search for other works by this author on: Oxford Academic PubMed Google Scholar H. Donis-Keller H. Donis-Keller * 1Genetics, St. LouisMO 63110, USA * To whom correspondence should be addressed Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 20, Issue 5, 11 March 1992, Page 1168, https://doi.org/10.1093/nar/20.5.1168 Published: 11 March 1992
Journal Article Two chromosome 7 dinucleotide repeat polymorphisms at gene loci epidermal growth factor receptor (EGFR) and proα2 (1) collagen (COL1A2) Get access D.D. Chi, D.D. Chi Search for other works by this author on: Oxford Academic PubMed Google Scholar A.V. Hing, A.V. Hing 1Departments of Genetics, Washington University School of MedicineSt Louis, MO 63110, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar C. Helms, C. Helms 1Departments of Genetics, Washington University School of MedicineSt Louis, MO 63110, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar T. Steinbrueck, T. Steinbrueck 1Departments of Genetics, Washington University School of MedicineSt Louis, MO 63110, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Santosh K. Mishra, Santosh K. Mishra 1Departments of Genetics, Washington University School of MedicineSt Louis, MO 63110, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Helen Donis-Keller Helen Donis-Keller * 1Departments of Genetics, Washington University School of MedicineSt Louis, MO 63110, USA *To whom correspondence should be addressed Search for other works by this author on: Oxford Academic PubMed Google Scholar Human Molecular Genetics, Volume 1, Issue 2, May 1992, Page 135, https://doi.org/10.1093/hmg/1.2.135 Published: 01 May 1992
Multiple tumor suppressor genes are implicated in the oncogenesis and progression of invasive carcinoma of the breast. To investigate the chronology of genetic changes we studied loss of heterozygosity on chromosome 17 in ductal carcinoma in situ, a preinvasive breast cancer. A microdissection technique was used to separate tumor from normal stromal cells prior to DNA extraction and loss of heterozygosity was assayed mainly using simple sequence repeat polymorphism markers and the polymerase chain reaction. Loss of heterozygosity on 17p was observed in 8 of 28 tumors (29%) when compared with normal control DNA, whereas no loss was seen on 17q, suggesting that at least one locus on 17p is involved early in the development of breast cancer.
