Ting Wu

Jiangsu University

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Nanfang Li

People's Hospital of Xinjiang Uygur Autonomous Region

Qing Zhu

People's Hospital of Xinjiang Uygur Autonomous Region

Zhonghai Yu

Shanghai Sixth People's Hospital

Jun Xiang

Eye & ENT Hospital of Fudan University

Mulalibieke Heizhati

People's Hospital of Xinjiang Uygur Autonomous Region

Wen Wang

Nanjing Medical University

Xiangting Li

Shanghai Municipal Center For Disease Control Prevention

Qingguo Meng

Nanjing Normal University

Shasha Liu

People's Hospital of Xinjiang Uygur Autonomous Region

Delian Zhang

People's Hospital of Xinjiang Uygur Autonomous Region

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Chinese Academy of Sciences

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Fudan University

Sun Yat-sen University

People's Hospital of Xinjiang Uygur Autonomous Region

Shanghai Jiao Tong University

University of Chinese Academy of Sciences

Broad Institute

Chinese Academy of Medical Sciences & Peking Union Medical College

Sichuan University

Zhejiang University

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ヒト気管支上皮細胞のTNF-α誘発アポトーシスの阻害におけるAspergillus fumigatus分生子の研究【Powered by NICT】

Jiefangjun Yixue Zazhi (2014)

Sun He Quan Wang Ting Wu Xiaodong Wu Xin Su

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Zinc-doped Prussian blue nanoparticles for mutp53-carrying tumor ion interference and photothermal therapy

Asian Journal of Pharmaceutical Sciences (2022)

Quanyi Jin Wenbao Zuo Qian Lin Ting Wu Cong Liu

Prussian blue

10.1016/j.ajps.2022.07.003

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Axial gradient excitation accelerates volumetric imaging of two-photon microscopy

Photonics Research (2022)

Yufeng Gao Xianyuan Xia Lina Liu Ting Wu Tingai Chen

Two-photon excitation fluorescence microscopy (TPM), owing to its capacity for subcellular resolution, intrinsic optical sectioning, and superior penetration depth in turbid samples, has revolutionized biomedical research. However, its layer-by-layer scanning to form a three-dimensional image inherently limits the volumetric imaging speed and increases phototoxicity significantly. In this study, we develop a gradient excitation technique to accelerate TPM volumetric imaging. The axial positions of the fluorophores can be decoded from the intensity ratio of the paired images obtained by sequentially exciting the specimen with two axially elongated two-photon beams of complementary gradient intensities. We achieved a 0.63 μm axial localization precision and demonstrate the flexibility of the gradient TPM on various sparsely labeled samples, including bead phantoms, mouse brain tissues, and live macrophages. Compared with traditional TPM, our technique improves volumetric imaging speed (by at least sixfold), decreases photobleaching (i.e., less than 2.07 ± 2.89 % in 25 min), and minimizes photodamages.

Photobleaching

Two-photon excitation microscopy

10.1364/prj.441778

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A Case of Follicular Mucinosis

The Chinese Journal of Dermatovenereology (2010)

Ting Wu

A 13-year-old boy presented with infiltrated plaque and alopecia of the scalp for 6 months.Physical examination shows:a 1.5cm×1.5cm red circular plaque with tiny scaling and shedding hair on the post-occipital scalp.Histopathological lesions of the formation of hair follicles mouth bolt,the dermal sebaceous glands within the hair follicle cells in a large number of light blue material deposition,ranging from the amount of perivascular inflammatory cell infiltration;Assi blue stain-positive.Diagnosis of follicular mucinosis was made based on histopathological findings.

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Histopathological examination

Infiltration (HVAC)

Vellus hair

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Study on the method of bacterial endotoxin test of Puerarin for Injection

China Medical Herald (2010)

Ting Wu

Objective:To establish a method for bacterial endotoxin test of Puerarin for Injection.Methods:According to the method of bacterial endotoxin test approved by Chinese Pharmacopoeia 2005 edition.Results:The interference on bacterial endotoxin test can be excluded when Puerarin for Injection was diluted below 1.5 mg/ml.Conclusion:It is feasi-ble to detect the bacterial endotoxin of Puerarin for Injection by the method of bacterial endotoxin test and the method may replace pyrogen test.

Puerarin

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Supplementary Data from Inhibition of AKT Sensitizes Cancer Cells to Antineoplastic Drugs by Downregulating Flap Endonuclease 1

Hong Zhu Congye Wu Ting Wu Wen Xia Shusheng Ci

<p>Figure S1-S5</p>

10.1158/1535-7163.22510342.v1

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Computational correction of copy number effect improves specificity of CRISPR–Cas9 essentiality screens in cancer cells

Nature Genetics (2017)

Robin M. Meyers Jordan Bryan James M. McFarland Barbara A. Weir Ann E. Sizemore

Subgenomic mRNA

10.1038/ng.3984

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Propofol Inhibits Biological Function of Hepatocellular Carcinoma Cells through LINC00475-Mediated Sonic Hedgehog Pathway

Pharmacology (2022)

Jiane Wu Fucheng Zhou Shangshu Lai Wenhua Wang Ting Wu

Hepatocellular carcinoma (HCC) is the most deadly cancer. Many signal pathways are implicated in HCC development, including sonic hedgehog (SHH). Propofol is an anesthetic commonly used in surgery. Recent studies have reported that propofol inhibits tumorigenesis and the development of HCC in a dose-dependent manner. The study aimed to identify the mechanism of how the propofol-mediated SHH-signaling molecule works in HCC.Cell proliferation, apoptosis, and invasion were examined, respectively, through colony formation, TUNEL, caspase-3 activity, and transwell assays. Protein levels of SHH, Ptch1, Smo, and Gli1 were determined via Western blot.Propofol could inhibit cell proliferation, migration, and invasion and induce apoptosis via suppression on SHH to inactivate the SHH pathway. By mechanistic assays, miR-340-5p was identified to target SHH and negatively regulate SHH. Long intergenic non-protein coding RNA 475 (LINC00475) was the endogenous sponge of miR-340-5p to upregulate SHH. Finally, the rescue assays were implemented. The activator of the SHH pathway completely rescued the effects of LINC00475 and SHH in propofol-induced HCC cells.Propofol inhibits HCC cell malignant behaviors via repressing LINC00475 to suppress SHH, thus inactivating the SHH pathway. These new findings might contribute to the understanding and application of propofol in HCC.

GLI1

10.1159/000527200

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GW25-e2301 Using three-dimensional speckle-tracking echocardiography to assess the left ventricular function of varying degrees aortic stenosis

Journal of the American College of Cardiology (2014)

Ting Wu Bai Wenjuan Hong Tang Li Rao

Ventricular Function

Speckle tracking echocardiography

10.1016/j.jacc.2014.06.1082

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