The subcellular distribution of Dp71 isoforms alternatively spliced for exon 71 and/or 78 was examined. The cDNA sequence of each variant was fused to the C‐terminus of the green fluorescent protein and the constructs were transfected transiently in the cell lines HeLa, C2C12 and N1E‐115. The subcellular distribution of the fused proteins was determined by confocal microscope analysis. The Dp71 isoform lacking the amino acids encoded by exons 71 and 78 was found exclusively in the cytoplasm whereas the variants containing the amino acids encoded by exon 71 and/or exon 78 show a predominant nuclear localization. The nuclear localization of Dp71 provides a new clue towards the establishment of its cellular function.

10.1016/s0014-5793(00)02044-5

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Citations (37)

Properties of theβ-glucosidase fromCellulomonas flavigena and fromEscherichia coli harboring the recombinant plasmid pJS3

Journal of Industrial Microbiology (1992)

Jesus Silva Ignacio Magaña V. Barajas Cecilia Montáñez

Beta-glucosidase

10.1007/bf01569764

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Citations (0)

Pattern of deletions of the dystrophin gene in Mexican Duchenne/Becker muscular dystrophy patients: The use of new designed primers for the analysis of the major deletion “hot spot” region

American Journal of Medical Genetics (1997)

Ramón Mauricio Coral‐Vázquez Diego Arenas Bulmaro Cisneros Laura Peñaloza F Salamanca

10.1002/(sici)1096-8628(19970613)70:3<240::aid-ajmg5>3.3.co;2-y

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[Restoration of transcription antitermination of N- bacteriophages in Escherichia coli with a mutant RNA polymerase].

PubMed (1994)

Bulmaro Cisneros Sergio Vaca Luis Sosa Cecilia Montáñez

The N protein of bacteriophage lambda modifies Escherichia coli RNA polymerase in such a way that it transcribes through termination signals, in a process called antitermination. In general N-mutants are not able to perform transcription antitermination. In this paper we report the suppression of N7 and Nmar3 mutations by Escherichia coli ron-lon strain. The lon mutation causes the N protein half-life to raise, suggesting that excess of N7 fragment or Nmar3 protein overcome the defect in antitermination. Under these conditions the lambda N-phages produced a titer similar to lambda wild type, although the plaques were smaller. These observations highlight the relevance of N half life in the regulation of transcription antitermination.

Antitermination

Transcription

Lambda phage

Source

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Embryonic neural stem/progenitor cells as model to characterize dystrophin and dystrophin-associated proteins expression during neuronal or astrocytic differentiation

MethodsX (2021)

José Romo‐Yáñez Griselda Rodríguez‐Martínez Jorge Aragón Lourdes Siqueiros‐Márquez Alma Herrera‐Salazar

Neural stem/progenitor cells (NSPC) are multipotent cells that renew themselves and could differentiate into neurons and macro glia (astrocytes and oligodendrocytes) of the nervous system during embryonic development. Duchenne muscular dystrophy is a severe type of muscular dystrophy caused by mutations in the dmd gene, and one-third of patients cursed with neuro-cognitive impairments. In this data article, we take advantage of the differentiation capacity of NSPC as a model to increase our knowledge in the neuronal and/or astrocytic differentiation and to evaluate the expression of dystrophins and dystrophin-associated proteins. We showed the characterization of undifferentiated and neuron and/or astrocyte differentiated NSPC. In addition, we evaluated the expression and subcellular localization of dystrophins and β-dystroglycan in undifferentiated NSPC and differentiated to neurons and astrocytes.•Primary culture of NSPC was characterized by the expression of multipotent markers nestin and Sox2.•Neuronal or astrocytic differentiation of NSPC was performed by basic fibroblast growth factor (FGF2) withdrawal, histamine or ciliary neurotrophic factor (CNTF) treatment, and expression of βIII-tubulin or glial fibrillary acidic protein (GFAP) as differentiation markers for neurons or astrocytes was evaluated.•This study will contribute to the understanding of dystrophins and dystrophin-associated proteins expression and function during neuronal or astrocytic differentiation of NSPC

Ciliary neurotrophic factor

Neuroepithelial cell

10.1016/j.mex.2021.101325

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Citations (2)

EF-hand domains are involved in the differential cellular distribution of dystrophin Dp40

Neuroscience Letters (2015)

Jorge Aragón Alejandro Martínez-Herrera Rosa María Bermúdez‐Cruz Ma. Luisa Bazán Gabriela Soid‐Raggi

Cajal body

Immunofluorescence

10.1016/j.neulet.2015.05.038

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Citations (13)