Ultrasound targeted microbubble destruction (UTMD) was introduced as a promising method to improve anti-tumor therapeutic efficacy, while minimizing side effects to healthy tissues. Nevertheless, the acoustical phenomenon behind the UTMD as well as the exact mechanisms of autophagy action involved in the increased anti-cancer response are still not fully understood. Therefore, we examined the drug resistance-reversing effects of low-intensity focused ultrasound with microbubble (LIFU+MB) in paclitaxel (PTX)-resistant ovarian cancer cells. Cell viability was evaluated using CCK8 (Cell Counting Kit-8), apoptosis was detected by flow cytometry, quantitative real-time PCR and Western blot were used to detect the expressions of mRNA and protein, and autophagy was observed by transmission electron microscopy (TEM). We revealed that the level of autophagy was increased (p < 0.05) in PTX-resistant ovarian cancer cells. Treatment of LIFU+MB combined with PTX can notably inhibit proliferation as well as increase apoptosis (p < 0.01) in drug-resistant cells. We proposed that LIFU+MB might affect the sensitivity of ovarian cancer cells to PTX by modulating autophagy. To verify the hypothesis, we analyzed the autophagy level of drug-resistant cells after the treatment of LIFU+MB and found that autophagy was significantly inhibited. Altogether, our findings demonstrated that LIFU+MB could reverse PTX resistance in ovarian cancer via inhibiting autophagy, which provides a novel strategy to improve chemosensitivity in ovarian cancer.
Background: Glioma is a common type of tumor in the central nervous system characterized by high morbidity and mortality. Autophagy plays vital roles in the development and progression of glioma, and is involved in both normal physiological and various pathophysiological progresses. Patients and Methods: A total of 531 autophagy-related genes (ARGs) were obtained and 1738 glioma patients were collected from three public databases. We performed least absolute shrinkage and selection operator regression to identify the optimal prognosis-related genes and constructed an autophagy-related risk signature. The performance of the signature was validated by receiver operating characteristic analysis, survival analysis, clinic correlation analysis, and Cox regression. A nomogram model was established by using multivariate Cox regression analysis. Schoenfeld's global and individual test were used to estimate time-varying covariance for the assumption of the Cox proportional hazard regression analysis. The R programming language was used as the main data analysis and visualizing tool. Results: An overall survival-related risk signature consisting of 15 ARGs was constructed and significantly stratified glioma patients into high- and low-risk groups ( P < 0.0001). The area under the ROC curve of 1-, 3-, 5-year survival was 0.890, 0.923, and 0.889, respectively. Univariate and multivariate Cox analyses indicated that the risk signature was a satisfactory independent prognostic factor. Moreover, a nomogram model integrating risk signature with clinical information for predicting survival rates of patients with glioma was constructed (C-index=0.861± 0.024). Conclusion: This study constructed a novel and reliable ARG-related risk signature, which was verified as a satisfactory prognostic marker. The nomogram model could provide a reference for individually predicting the prognosis for each patient with glioma and promoting the selection of optimal treatment. Keywords: glioma, autophagy, risk signature, prognosis, nomogram
Abstract Objective To construct a predictive model for AMH levels in women of reproductive age using multiple linear regression analysis. Methods A retrospective study was conducted involving 492 patients who underwent ovarian reserve function tests at Hangzhou Traditional Chinese Medicine Hospital. The patients were randomly divided into a training set (n = 420) and a validation set (n = 72). All subjects underwent transvaginal 3D ultrasound to measure AFC, OV, PSV, and RI. Additionally, fasting venous blood samples were collected on days 2–5 of the menstrual cycle for AMH level testing. Pearson correlation analysis was used to assess the relationship between age, AFC, OV, PSV, RI values, and AMH. Multiple linear regression analysis was employed to construct both a unified regression model and a subgroup regression model for prediction. Model performance was evaluated by comparing RMSE and MAE. Results AMH showed a negative correlation with age and positive correlations with OV, AFC, and PSV. The RMSE and MAE of the subgroup regression prediction model were lower than those of the unified regression prediction model. Conclusion The subgroup regression AMH prediction model developed through multiple linear regression analysis performs well and can provide accurate individualized predictions.
Large fetal head and neck tumors are being increasingly identified during prenatal examination and tend to have a poor prognosis. Nevertheless, appropriate intrauterine interventions at suitable periods can improve pregnancy outcome. Ultrasound-guided puncture biopsy of the solid fetal head and neck mass and radiofrequency ablation of a portion of the tissue can clarify the tumor pathology and reduce the tumor size, respectively. These treatment methods are reproducible and associated with reduced trauma and complications.
With force transducer and displacement transducer automatically measuring the test load and the depth of diamond pyramid pressed mark respectively, the data are displayed to control the test processing and assure effectiveness of the testing. As soon as unloaded, the depth of the remainder pressed mark is measured on line by the displacement transducer automatically. Furthermore, the calculation of Vickers hardness number and its output are carried out automatically so as to ensure the testing results are obtained on real_time. This is the most concise and effective way for Vickers hardness tester. Thus, not only it will be universally used in routine inspection of metal's hardness easily and quickly, but also be used to solve the technical problem that put the unitary scale of hardness into practice.
To investigate the application and the related influencing factors of ultrafast pulse wave velocity (ufPWV) in the evaluation of carotid artery elasticity in patients with Immunoglobulin A nephropathy (IgAN).
The author,through an exposition of the operation status of SINOPEC,points out that SINOPEC has possessed the power to join international competition.He further analyses the foreign corporations' contending for oil product market in China,pointing out the necessity for SINOPEC to promote internationalized operation.While giving an introduction of oil product market in Europe and America,approximate estimation of profit and analysis of the tariff on oversea oil product market,the author points out the feasibility of the construction of oversea gas stations.
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