Evehe Bebandoue Marie-Solange

Université de Yaoundé I

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Kayla Laserson

Division of Parasitic Diseases and Malaria

Patrice N. Mimche

University of Utah

Dyann F. Wirth

Broad Institute

Johanna P. Daily

Albert Einstein College of Medicine

Akindeh M. Nji

Université de Yaoundé I

Palmer Masumbe Netongo

Université de Yaoundé I

Songmbe Michael Yong

University of Buea

Immaculate Amunom

Sekisui XenoTech (United States)

Peter Enyong

University of Buea

Vincent P.K. Titanji

University of Buea

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Université de Yaoundé I

University of Buea

Centers for Disease Control and Prevention

University of Utah

Harvard University

Harvard University Press

Fobang Foundation

New York Blood Center

Institut de Recherches Médicales et d’Etudes des Plantes Médicinales

Division of Parasitic Diseases and Malaria

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Limited variation of the 5'cis-control region of the transmission blocking vaccine candidate Pfs25 amid great genetic diversity of Plasmodium falciparum in Cameroon

Wilfred Fon Mbacham Patrice N. Mimche Palmer Masumbe Netongo Evehe Bebandoue Marie-Solange Akindeh M. Nji

Genetic recombination during sexual reproduction within Plasmodium sp. contributes to parasite diversity and altered gene expression of certain surface markers. The pfs25 gene involved in the upset of gametocytogenesis is a candidate antigen in transmission blocking vaccine. This study investigated the polymorphism of Pfs25 within its 5’cis-control region in field isolates from different ecotypes in Cameroon. Symptomatic patients and asymptomatic healthy school children with a positive smear and from different ecozones were included. Parasite DNA was extracted and polymorphisms within pfs25, cg2-, msp-1, msp-2 and glurp genes were investigated by PCR-RFLP and DNA sequencing. Putative control elements of the 5’cis control regions of Pfs25 were identified by PCGENE software and enzymes were selected whose sequences produced or abolished restriction sites by mutations. Malaria infection was mainly caused by Plasmodium falciparum with sporadic occurrence of Plasmodium malariae and Plasmodium ovale. Analysis of the Pfs25 5’ cis-control region identified only one polymorphism (0.002%) that abolished an RsaI restriction site as part of the sequence TTTCTGTAC, located 40 bp downstream of the promoter and found at – 478 bp of the ATG. Analysis of the 5’ ciscontrol sequence of Pfs25 revealed minimal variation of the promoter region amid great zonal differences in parasite population. Altitudinal differences in parasite populations were not easily discernable.

10.5897/ajb07.715

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