Marta Lemme

Nanion (Germany)

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Arne Hansen

German Centre for Cardiovascular Research

Thomas Eschenhagen

German Centre for Cardiovascular Research

Michael George

Nanion (Germany)

Niels Fertig

Nanion (Germany)

Dorit Schuster

University Hospital Schleswig-Holstein

Ole Ammerpohl

Universität Ulm

Thomas Pickardt

German Centre for Cardiovascular Research

Elena Dragicevic

Nanion (Germany)

Bärbel Ulmer

Universität Hamburg

Kerstin Runde

University Hospital Schleswig-Holstein

Cooperative Institutions

German Centre for Cardiovascular Research

University Medical Center Hamburg-Eppendorf

Universität Hamburg

Nanion (Germany)

University Hospital Schleswig-Holstein

University of Lübeck

Kiel University

Institute of Experimental Pharmacology and Toxicology of the Slovak Academy of Sciences

Cardiovascular Research Center

Ludwig-Maximilians-Universität München

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Additional file 17: of DNA methylation profiling allows for characterization of atrial and ventricular cardiac tissues and hiPSC-CMs

Figshare (2019)

Kirstin Hoff Marta Lemme Anne‐Karin Kahlert Kerstin Runde Enrique Audain

Table S4. Primers for qPCR. (XLSX 13 kb)

Profiling (computer programming)

10.6084/m9.figshare.8261021

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Beating heart on a chip: a novel microfluidic platform to generate functional 3D cardiac microtissues

Lab on a Chip (2015)

Anna Marsano Chiara Conficconi Marta Lemme Paola Occhetta Emanuele Gaudiello

The beating heart-on-a-chip (i) generates 3D cardiac constructs with well-defined geometries from cell-laden hydrogel prepolymers, (ii) provides uniaxial cyclic mechanical stimulation, (iii) allows efficient delivery of drugs and chemicals.

Microfluidic chip

Cardiac cell

10.1039/c5lc01356a

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Citations (376)

Cell monitoring using multi-frequency impedance recordings for label-free and time-resolved cell response analysis

Journal of Pharmacological and Toxicological Methods (2021)

Sonja Stoelzle‐Feix Krisztina Juhász Michael Skiba Joachim Wegener Ronald J. Knox

10.1016/j.vascn.2021.106969

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Cell Banking of hiPSCs: A Practical Guide to Cryopreservation and Quality Control in Basic Research

Current Protocols in Stem Cell Biology (2020)

Aya Shibamiya E. Schulze Dana Krauß Christa Augustin Marina Reinsch

Abstract The reproducibility of stem cell research relies on the constant availability of quality‐controlled cells. As the quality of human induced pluripotent stem cells (hiPSCs) can deteriorate in the course of a few passages, cell banking is key to achieve consistent results and low batch‐to‐batch variation. Here, we provide a cost‐efficient route to generate master and working cell banks for basic research projects. In addition, we describe minimal protocols for quality assurance including tests for sterility, viability, pluripotency, and genetic integrity. © 2020 The Authors. Basic Protocol 1 : Expansion of hiPSCs Basic Protocol 2 : Cell banking of hiPSCs Support Protocol 1 : Pluripotency assessment by flow cytometry Support Protocol 2 : Thawing control: Viability and sterility Support Protocol 3 : Potency, viral clearance, and pluripotency: Spontaneous differentiation and qRT‐PCR Support Protocol 4 : Identity: Short tandem repeat analysis

Viability assay

10.1002/cpsc.127

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Citations (15)

Tackling chronic compound responses of hiPSC-CMs for preclinical cardiac risk evaluation: defined serum-free medium and long-term culture on the FLEXcyte 96

Biophysical Journal (2022)

Matthias Goßmann Bettina Lickiss Marta Lemme Elena Dragicevic Ravi Vaidyanathan

Medium term

10.1016/j.bpj.2021.11.1568

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Design of a microfluidic device for cyclic mechanical stimulation of three dimensional cardiac cell constructs

Marta Lemme Chiara Conficconi

Cardiac cell

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Micro-scale engineered tissues as in vitro cardiac scar-like model

Paola Occhetta Giuseppe Isu Giulia Cerino Chiara Conficconi Marta Lemme

Source

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Drug Effects On Human Na(+)/Ca(2+)Exchanger and Implications for Drug Development

Biophysical Journal (2021)

Maria Barthmes Andre Bazzone Marta Lemme Sonja Stoelzle‐Feix Andrea Brüggemann

Sodium-calcium exchanger

HEK 293 cells

Homeostasis

Safety pharmacology

10.1016/j.bpj.2020.11.666

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Atrial-like Engineered Heart Tissue: An In Vitro Model of the Human Atrium

Stem Cell Reports (2018)

Marta Lemme Bärbel Ulmer Marc D. Lemoine Antonia T.L. Zech Frederik Flenner

Cardiomyocytes (CMs) generated from human induced pluripotent stem cells (hiPSCs) are under investigation for their suitability as human models in preclinical drug development. Antiarrhythmic drug development focuses on atrial biology for the treatment of atrial fibrillation. Here we used recent retinoic acid-based protocols to generate atrial CMs from hiPSCs and establish right atrial engineered heart tissue (RA-EHT) as a 3D model of human atrium. EHT from standard protocol-derived hiPSC-CMs (Ctrl-EHT) and intact human muscle strips served as comparators. RA-EHT exhibited higher mRNA and protein concentrations of atrial-selective markers, faster contraction kinetics, lower force generation, shorter action potential duration, and higher repolarization fraction than Ctrl-EHTs. In addition, RA-EHTs but not Ctrl-EHTs responded to pharmacological manipulation of atrial-selective potassium currents. RA- and Ctrl-EHTs' behavior reflected differences between human atrial and ventricular muscle preparations. Taken together, RA-EHT is a model of human atrium that may be useful in preclinical drug screening.

Atrium (architecture)

Atrial action potential

10.1016/j.stemcr.2018.10.008

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Citations (130)

Política Comercial

Elsevier eBooks (2013)

Jorge Chami Batista Marta Lemme

10.1016/b978-85-352-6368-8.00025-6

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