Jianguo Sun

China Pharmaceutical University

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Guangji Wang

China Pharmaceutical University

103

Ying Peng

State Key Laboratory of Natural Medicine

Jiye Aa

China Pharmaceutical University

Haiping Hao

China Pharmaceutical University

Bei Yan

Hunan Agricultural University

Haitang Xie

Wannan Medical College

Fang Zhou

China Pharmaceutical University

Jingwei Zhang

Shanghai Ninth People's Hospital

Yi Gu

University of Electronic Science and Technology of China

Junrong Zhu

Fuzhou University

Cooperative Institutions

China Pharmaceutical University

199

Nanjing Medical University

Chinese Academy of Sciences

Zhejiang University

Chinese Academy of Medical Sciences & Peking Union Medical College

State Key Laboratory of Natural Medicine

Sun Yat-sen University

Sichuan University

Peking University

Shanghai Jiao Tong University

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Quantitative determination of metformin, saxagliptin and 5-hydroxy saxagliptin simultaneously by hydrophilic interaction liquid chromatography - electrospray ionization mass spectrometry and its application to a bioequivalence study with a single-pill combination in human

Journal of Chromatography B (2018)

Ying Peng Qingqing Chang Na Yang Shiyin Gu Yi Zhou

Saxagliptin

Bioequivalence

10.1016/j.jchromb.2018.02.007

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Citations (17)

Progress in pharmacodynamics and pharmacokinetics of ginsenoside Rg1

Zhongguo yaoke daxue xuebao (2007)

Jianguo Sun

Panax ginseng is a highly valuable herb in Asia,containing many constituents,of which the majority are ginsenosides.For the purpose of providing thoughts and methods on the study of ginsenosides,the research progress of ginsenoside Rg1,a representative,in the pharmacology of central nervous system,cardiovascular system,endocrine system and immune system are reviewed,and the possible mechanism and application are discussed in this paper.Besides,the advances in pharmacokinetics of Rg1 such as absorption and metabolism are also summarized.On this foundation,the pharmacological characteristics and pharmacokinetic behaviors are compared and correlated for further in-depth research.

Ginsenoside Rg1

Pharmacodynamics

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Citations (7)

Pharmacokinetic characterization of ginsenoside Rh2, an anticancer nutrient from ginseng, in rats and dogs

Food and Chemical Toxicology (2009)

Yi Gu Guangji Wang Jianguo Sun Yuanwei Jia Wei Wang

Membrane permeability

Intestinal Permeability

10.1016/j.fct.2009.06.013

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Citations (115)

Imperatorin induces Mcl-1 degradation to cooperatively trigger Bax translocation and Bak activation to suppress drug-resistant human hepatoma

Cancer Letters (2014)

Xia Li Xueli Zeng Jianguo Sun Hua Li Ping Wu

Imperatorin

10.1016/j.canlet.2014.03.017

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Citations (48)

HPLC Assay of Levamisole and Abamectin in Sheep Plasma for Application to Pharmacokinetic Studies

Journal of Liquid Chromatography &amp Related Technologies (2006)

Peyami Sari Jianguo Sun Majid Razzak Ian G. Tucker

Abstract High performance liquid chromatography (HPLC) techniques were developed to quantify levamisole and abamectin in sheep plasma. UV detection (225 nm) for levamisole and fluorescence detection (excitation at 365 nm and emission at 470 nm) for abamectin were used. Separation was achieved on a C18 Prodigy ODS column with a mobile phase of phosphate (NaH2PO4 and Na2HPO4)‐acetonitrile (60∶40, v/v) (pH 7.5; 0.01M) for levamisole and methanol‐acetonitrile‐water (95∶3∶2, v/v/v) for abamectin. The retention times were 5.7 minutes for levamisole phosphate, 4.9 minutes for abamectin, 7.4 minutes internal standard (ivermectin). Calibration curves for levamisole phosphate and abamectin were linear over the range 0.05–10 µg/mL for levamisole phosphate and 0.25–20 ng/mL for abamectin, with the correlation coefficients for both drugs exceeding >0.999. The LOQ was achieved as the lowest point on the standard curve, 0.05 µg/mL for levamisole phosphate with the RSD 18.2% and 0.25 ng/mL for abamectin with the RSD 19.6%. The maximum intra‐day and inter‐day coefficients of variation were 9.1% and 15.0%, respectively, at 0.1 µg/mL (lowest concentration) for levamisole phosphate, and 11.9% and 19.3%, respectively, at 0.5 ng/mL (lowest concentration) for abamectin. Accuracies were 107.5% and 91.9% for levamisole and abamectin, respectively, at lowest concentrations of 0.1 µg/mL for levamisole phosphate and 1 ng/mL for abamectin. The recoveries from frozen and thawed plasma samples were 86.3% at 0.1 µg/mL for levamisole phosphate, and 105.8% at 2 ng/mL for abamectin. Both methods were successfully applied for analysis of levamisole and abamectin in plasma after subcutaneous injection to sheep of a formulation of medium chain mono‐ and diglyceride‐propylene glycol‐glycerol formal containing both levamisole phosphate and abamectin.

Abamectin

Levamisole

10.1080/10826070600832954

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Citations (22)

[Pharmacokinetics of oxymatrine and its metabolite in beagle dogs by LC-MS].

PubMed (2005)

Sujun Wang Guangji Wang Xiaotian Li Ren-ling Ma Jianguo Sun

To establish LC-MS method in the determination of oxymatrine and its metabolite in plasma and investigate their pharmacokinetics in beagle dogs.Lichrospher C18 column (4.6 mm x 250 mm, 5 microm) was used as the analytical column maintained at 25 degrees C. The mobile phase consisted of 10 mmol x L(-1) CH3COONH4 and CH3OH (25:75). Flow rate was 1 mL x min(-1). Electrospray ionization (ESI) was carried out. The ESI ion source was set in positive ion polasity mode. The selective ion monitoring (SIM) was set at m/z 265.1 and 249.2.The linearity ranged from 2 to 5000 ng x mL(-1) (r = 0.9991). The detection of oxymatrine and its metabolite were 0.6 and 0.3 ng x mL(-1). The RSD(%) within day and between day was less than 4.7%. The recovery of this method was more than 96.5%. The disposition was conformed to a two-compartment model. The T(1/2), Tmax, Cmax, MRT, AUC(0-->24 h) of oxymatrine were (5.5+/-1.58) h, (1.0+/-0.30) h, (2418.3 +/-970.78) ng x mL(-1), (3.2+/-0.64) h, (5797.4+/-908.16) ng x mL(-1) x h accordingly. The corresponding T(1/2), Tmax, Cmax, MRT, AUC(0-->24 h) of matrine were (9.8+/-2.77) h, (1.9+/-1.09) h, (1532.4+/-494.86) ng x mL(-1), (4.4+/-1.97) h, (5530.5+/-1042.65) ng x mL(-1) x h.This assay was highly sensitive, rapid, simple and specific enough for determining concentrations of oxymatrine and its metabolite matrine in plasma of beagle dog.

Beagle

Oxymatrine

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Citations (3)

Intestinal absorption mechanisms of ginsenoside Rh2: stereoselectivity and involvement of ABC transporters

Xenobiotica (2010)

Yi Gu G.-J. Wang Xiujie Wu Y.-T. Zheng J.-W. Zhang

This study investigated the absorption mechanism of ginsenoside Rh2 to clarify the reasons for its poor absorption. Transepithelial transport across Caco-2 cell monolayers, cellular uptake, and in situ rat intestinal perfusion were examined. Cellular uptake of Rh2 was linear from 1 to 50 μM at 4°C, whereas it was saturated when the concentration exceeded 10 μM at 37°C. At 37°C, the uptake at 10 μM was linear in 60 min. Intracellular exposure in 240 min was 2173.70 and 979.38 ng·min/μg for S and R isomers, respectively. Transepithelial permeability of Rh2 was about 10⁻⁸ to 10⁻⁷ cm/s. Efflux ratios were above 1.5. Sodium dodecyl sulfate, sodium citrate, and sodium deoxycholate had no effect on Rh2 permeability. After intestinal perfusion for 3 h, 9.1% of 20(R)-Rh2 and 15.7% of 20(S)-Rh2 were absorbed. Cyclosporine, quercetin, and probenecid could improve the cellular uptake, absorptive permeability, and intestinal absorption. Carrier-mediated transport was the major absorption mechanism. Rh2 was a substrate of ABC transporters. The ABC-transporter-mediated efflux and the poor permeability were the major reasons for Rh2 poor absorption. The stereoselective absorption was significant. R isomer exhibited lower absorption profiles in all the experiments, possibly due to more potent efflux.

Efflux

Caco-2

Probenecid

Intestinal Permeability

10.3109/00498254.2010.500744

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Citations (39)

Pregnancy-induced hypertension during cesarean section application propofol general anesthesia of remifentsnil

Guoji yiyao weisheng daobao (2012)

Li Boquan Ji-en Chen Lixia Yan Hong Liu Shanwen Li

Objective To investigate the feasibility and the impact of propofol remifentanil atracurium general anesthesia application to the pregnancy-induced hypertension in patients with cesarean section and its influence to mothers and infants.Methods 38 cases of pregnancy-induced hypertension cesarean section cases,the application of propofol remifentanil atracurium general anesthesia,the newborn was Apgar scored 1 minute after delivery,umbilical artery (U A) obtained within 2 minutes with 2 ml of blood samples for blood gas analysis.Results All patients had immediate tracheal intubation,skin incision,extubation hemodynamic stability at each time point,and within the normal range of fluctuation,no occurrence of intraoperative awareness found.Neonatal Apgar score was 8-10 minute after delivery,with all results of UA blood gas analysis pH value,PaO2(kpa),PaCO2(kpa),BE and HCO3- within the normal range.Conclusion Remifentanil propofol atracurium general anesthesia has good effect for patients with cesarean section,without adverse effects to the newborn. Key words: Pregnancy-induced hypertension; Cesarean section; Propofol remifentanil atracurium; General anesthesia

Apgar score

10.3760/cma.j.issn.1007-1245.2012.04.011

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Citations (0)

Metabonomic characterization of early atherosclerosis in hamsters with induced cholesterol

Biomarkers (2009)

Weibin Zha Jiye Aa Guangji Wang Bei Yan Shenghua Gu

Atherosclerosis is a complicated and multifactorial disease, induced not only by genotype, but also, even more importantly, by environmental factors. Study on the metabolic perturbation of endogenous compounds may offer deeper insight into development of atherosclerosis. Gas chromatography/mass spectrometry (GC/MS)-based metabonomics was used to profile a metabolic fingerprint of serum obtained from hamsters with induced cholesterol. The deconvoluted GC/MS data were processed by multivariate statistical analysis tools, such as principal component analysis (PCA) and partial least squares projection to latent structure and discriminant analysis (PLS-DA). For the first time we showed a time-dependent development of the model animal from normal to hypercholesterolaemia, and further to early atherosclerosis. Twenty-one compounds were identified as markers involved in the development to atherosclerosis. Identification of the compounds suggests that amino acid metabolism and fatty acid oxidation are significantly perturbed following cholesterol overloading. The data provide novel information to approach the pathophysiological processes of the hypercholesterolaemia and atherosclerosis disease continuum.

10.1080/13547500903026401

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Citations (31)

Quantitative determination of intracellular Asulacrine in MCF‐7 breast cancer cells by liquid chromatography–mass spectrometry and its application to cellular pharmacokinetic studies of P188 modified liposomes

Biomedical Chromatography (2016)

Xiaojie Zang Jingwei Zhang Yaqian Zhou Qianying Chen Ying Peng

Asulacrine (ASL), an analogue of amsacrine, has shown higher anti-breast and anti-lung cancer activity. Hereby, a new sensitive and selective liquid chromatography-mass spectrometry (LC/MS) method was developed to determine intracellular asulacrine. The chromatographic separation was performed on an Agilent Zorbax Extend-C18 column (2.1 mm i.d. × 50 mm, 5 μm) using gradient elution with water (2 mmol/L ammonium acetate and 0.1% acetic acid) and acetonitrile as the mobile phase. The detection was achieved with selected ion monitoring mode using electrospray ionization in positive mode with target ions at m/z 465.3 and m/z 326.1 for asulacrine and midazolam, respectively. The standard curve showed a good linearity with the lower limit of quantification of 1 ng/mL, as a result of which, the trace concentration of ASL in cell suspension could be quantified. The intra- and inter-day accuracy ranged from -5.28 to 6.5% and from -6.32 to 1.05%, and the intra- and inter-day precisions were no more than 7.65% and 11.71%, respectively. Additionally, no degradation of asulacrine was observed during stability evaluation. The method was proved to be powerful and practical to determine and compare the intracellular distribution and kinetics of ASL under different formulations in MCF-7 breast cancer cells.

MCF-7

10.1002/bmc.3762

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Citations (3)